• Journal of the Korea Society for Simulation
• /
• v.17 no.4
• /
• pp.167-174
• /
• 2008

The introduction of confocal microscopy makes it possible to observe the structural change of live neuronal cell. Neuro-degenerative disease, such as Alzheimer;s and Parkinson’s diseases are especially related to the morphological change of dendrite spine. That’s the reason for the study of segmentation and extraction from confocal microscope image. The difficulty comes from uneven intensity distribution and blurred boundary. Therefore, the image processing technique which can overcome these problems and extract the structural information should be suggested. In this paper, we propose robust structural information extracting technique with confocal microscopy images of dendrite in brain neurons. First, we apply the nonlinear diffusion filtering that enhance the boundary recognition. Second, we segment region of interest using iterative threshold selection. Third, we perform skeletonization based on Fast Marching Method that extracts centerline and boundary for analysing segmented structure. The result of the proposed method has been less sensitive to noise and has not been affected by rough boundary condition. Using this method shows more accurate and objective results.

• Journal of Biomedical Engineering Research
• /
• v.24 no.2
• /
• pp.77-82
• /
• 2003

Virtual colonoscopy is a non-invasive computerized procedure to detect polyps by examining the colon from a CT data set. To fly through the inside of colons. the extraction of a suitable flight-path is necessary to Provide the viewpoint and view direction of a virtual camera. However. manual path extraction by Picking Points is a very time-consuming and difficult task due 1,c, the long and complex shape of colon. Also, existing automatic methods are computationally complex. and tend to generate an improper and/or discontinuous path for complicated regions. In this paper, we propose a fast flight-path generation algorithm using the distance and order maps. The order map Provides all Possible directions of a path. The distance map assigns the Euclidean distance value from each inside voxel to the nearest background voxel. By jointly using these two maps. we can obtain a proper centerline regardless of thickness and curvature of an object. Also, we Propose a simple smoothing technique that guarantees not to collide with the surface of an object. The phantom and real colon data are used for experiments. Experimental results show that for a set of human colon data, the proposed algorithm can provide a smoothened and connected flight-path within a minute on an 800MHz PC. And it is proved that the obtained flight-Path provides successive volume-rendered images satisfactory for virtual navigation.