• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.197-203
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• 2001

A fusion protein, consisting of human epidermal growth factor as a recognition domain and human angiogenin as a toxin domain, can be used as a targeted therapeutic against breast cancer cells among others. The fusion protein was expressed as inclusion body in recombinant E. coli, and when the conventional, solution-phase refolding process was used the refolding yield was very low due to severe aggregation, probably due to the opposite surface charge due to vastly different pI values of each domain. Solid-phase refolding process exploiting ionic interactions between the solid matrix and the protein was tried, but the ionic binding yield was very low regardless of the resins and pH conditions used. To provide higher affinity toward the solid matrix, six lysine residues were tagged to the N -terminus of the hEGF domain When the cation exchange resins such as heparin- or CM-Sepharose were used as the matrix, the adsorption capacity increased 2.5-3 times and the subsequent refolding yield increased nearly IS times compared to the conventional process.

• Journal of Institute of Control, Robotics and Systems
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• v.21 no.6
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• pp.518-523
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• 2015

This paper proposes a gait phase classifier using a Recurrent Neural Network (RNN). Walking is a type of dynamic system, and as such it seems that the classifier made by using a general feed forward neural network structure is not appropriate. It is known that an RNN is suitable to model a dynamic system. Because the proposed RNN is simple, we use a back propagation algorithm to train the weights of the network. The input data of the RNN is the lower body's joint angles and angular velocities which are acquired by using the lower limb exoskeleton robot, ROBIN-H1. The classifier categorizes a gait cycle as two phases, swing and stance. In the experiment for performance verification, we compared the proposed method and general feed forward neural network based method and showed that the proposed method is superior.

• The Research Journal of the Costume Culture
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• v.14 no.5
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• pp.864-875
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• 2006

The characteristics of the demographics in Korea as it gets older are the increase of Elderly Women and continuous progress in urbanization. In this study, body shapes are classified as standard, obese, and tiny according to the previous studies based on the body shape characteristics and the body measurement of the Elderly Women. Based on the classification, we developed prototype of the panty for the Elderly Women to provide basic materials for the quality enhancement of the clothing of the increasing Elderly Women. The followings are the result of the study. 1. To categorize the body shapes of the Elderly Women focusing on the lower half, we grouped the target subjects using the nested approach by the average standard deviation and the factor analysis minimal diffusion method. Accordingly, type 1 and 2 had 36 members respectively and type 3 had 43 members. In this study, two Elderly Women subjects with standard body shape falling under the type 1 were selected as the subjects. 2. In the second trial evaluation for the prototype panty for the Elderly Women 32 items for appearance test and 3 items for functional test were evaluated. The scores in leg, sideline and hip were shown high and the balance between the parts was maintained very well. In the functional test, the panty used to be too tight for the leg curve but in the second trial it was improved, too. In each item, the second trial test showed better score than the first trial test. Conclusively, the most optimal panty prototype for the Elderly Women was proposed according to the trial test result.

• Toxicological Research
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• v.31 no.4
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• pp.371-392
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• 2015

TS-DP2 is a recombinant human granulocyte colony stimulating factor (rhG-CSF) manufactured by TS Corporation. We conducted a four-week study of TS-DP2 (test article) in repeated intravenous doses in male and female Sprague-Dawley (SD) rats. Lenograstim was used as a reference article and was administered intravenously at a dose of $1000{\mu}g/kg/day$. Rats received TS-DP2 intravenously at doses of 250, 500, and $1000{\mu}g/kg/day$ once daily for 4 weeks, and evaluated following a 2-week recovery period. Edema in the hind limbs and loss of mean body weight and body weight gain were observed in both the highest dose group of TS-DP2 and the lenograstim group in male rats. Fibro-osseous lesions were observed in the lenograstim group in both sexes, and at all groups of TS-DP2 in males, and at doses of TS-DP2 $500{\mu}g/kg/day$ and higher in females. The lesion was considered a toxicological change. Therefore, bone is the primary toxicological target of TS-DP2. The lowest observed adverse effect level (LOAEL) in males was $250{\mu}g/kg/day$, and no observed adverse effect level (NOAEL) in females was $250{\mu}g/kg/day$ in this study. In the toxicokinetic study, the serum concentrations of G-CSF were maintained until 8 hr after administration. The systemic exposures ($AUC_{0-24h}$ and $C_0$) were not markedly different between male and female rats, between the administration periods, or between TS-DP2 and lenograstim. In conclusion, TS-DP2 shows toxicological similarity to lenograstim over 4-weeks of repeated doses in rats.

• Journal of Acupuncture Research
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• v.26 no.4
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• pp.91-98
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• 2009

Objectives : The objective of this review is to introduce the basic concepts on the febrile disease in the Mongolian Traditional Medicine(MTM). Methods : We comprehensively reviewed related textbooks in Mongolian and articles searched from China National Knowledge Infrastructure(CNKI) on febrile disease about MTM and summarized the pathophysiologic basis of, and treatment principles in MTM. Results and Conclusions : 1. In MTM, there are basic concepts such as theory of Arga-Bilig, theory of five elements, theory of three elements(人體三素), theory of seven constitutions and three discharges(七元三濊). Most of all, theory of three elements, which is about the basic elements of human body in MTM, is important fundamental notions for explaining human physiology and pathology. In MTM, It is explained that the imbalance of three elements, namely khii, shar, bad-kan is the root of all disease. Especially, febrile disease is treated by suppressing activated shar and blood(血液), which have fire characters, and managing various additional symptoms by balancing three elements and eliminating miasma(邪氣), inappropriate substances such as bile water(shar yc, 黃水) or blood stasis(惡血) simultaneously. 2. In MTM, both of internal treatment modalities(various medicinal therapy) and external treatment modalities(physical therapy) have been used for febrile disease. Food therapy and life style modification also have been recognized as important treatment methods. These aspects correspond to wholism theory of Korean Traditional Medicine(KTM). Further studies about details of each treatment methods will discover new applicable treatment method and make broaden the external boundaries of the KTM.

• Journal of Korean Institute of Industrial Engineers
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• v.25 no.1
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• pp.56-66
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• 1999

Shoulder joint is the most movable joint in human body with, at least, three degrees of freedom, since there are at least three bones and five joints involved in shoulder movement. Due to the complexity of the shoulder joint and the lack of appropriate anatomical data, modeling of the shoulder joint has been known to be extremely difficult. In many biomechanical models being used, shoulder joint is considered as a fixed point and it is also assumed that the shoulder joint does not noticeably move during the shoulder movement. However, such an assumption is not valid in real applications and causes inaccuracy, especially, in the area of workspace evaluation. The reachable area generated by a human becomes somewhat different from that of current models for those models fail to appropriately reflect the movement of shoulder joint's center of rotation. In this study, the location of the shoulder joint's center of rotation was obtained in relation to the location of humerus, on which a new model for reach envelope generation was developed for workspace evaluation. From the experiments conducted for three subjects, the initial location of the center of rotation was determined for each subject and subsequent changes in the instantaneous center of rotation were drawn as a function of flexion and abduction of the shoulder. Based on the regression analysis, the study suggested a new method for the generation of reach envelope. Comparisons were also made among real reach envelopes obtained from the experiment, the ones from the model, and the ones from the new method suggested in the study. As a result, the prediction errors incurred from the new method were significantly reduced when compared to the ones from the current approach.

• Journal of Dairy Science and Biotechnology
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• v.35 no.3
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• pp.169-175
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• 2017

Recently, the health-promoting effects of functional foods have been shown to prevent nutrition-related diseases, improve physical and mental well-being, satisfy hunger, and provide necessary nutrients for the human body. Koumiss is a common type of fermented mare's milk that has been shown to have beneficial therapeutic effects on cardiovascular disease, tuberculosis, and diarrhea as it can nourish vessels, relieve ill mood, and improve digestion. Hence, in this study, we aimed to prepare health-promoting koumiss using different concentrations of flaxseed (Linum usitatissimmum L.), which has the potential to control heart disease, hypertension, inflammation, and lung function. The pH was decreased to around 4.42-4.43, whereas the total anthocyanin (TA) content was increased to around 0.78~0.82% after fermentation of the koumiss premix. There were no significant differences in pH and TA among the three groups, including the control group. In sensory profile analysis, the taste, flavor, color, texture, and overall acceptability decreased in proportion to the added amount of flaxseed. These findings provided the first data describing the effects of flaxseed on koumiss fermentation, establishing a basis for commercial-scale production of koumiss containing fermented flax seed and for improving the health-promoting activity of koumiss.

• Clinical and Experimental Reproductive Medicine
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• v.23 no.1
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• pp.87-94
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• 1996

For evaluating the suitability of human follicular fluid(HFF) as protein supplement in ART, this preliminary study was performed to examine the maturation promoting activity of HFF on mouse follicular oocytes in vitro. Mouse follicular oocytes were collected from ovaries of 21-28 day old ICR mice by puncturing the antral follicles with fine needle at 48 hours after PMSG injection. The oocytes were rinsed and cultured in modified Whittingham's $T_6$ medium containing purines or dbcAMP to maintain meiotic arrest, and different concentrations of HFF were added into the culture medium to examine the effect of HFF on releasing the oocytes from the suppressive influence of the meiotic inhibitors. As a control for HFF, the maturation promoting activity of human fetal cord serum(HFCS) was investigated and compared with the activity of HFF. While HFF was separated, by molecular weight(M.W), into high M.W. fraction(M.W>30,000) and low M.W. fraction(M.W<30,000) and the effects of the fractions on meiotic resumption were investigated in the presence of the meiotic inhibitors. Also hormone analysis was performed to compare the content of hormones in HFF with that in HFCS. Same concentrations of HFF and HFCS induced similar germinal vesicle break down(GVBD) rates of the oocytes meiotic arrested by purines(4mM hypoxanthine+0.75mM adenosine), but the extrusion rate of 1st polar body(PB) of the oocytes cultured in HFF(65.0%, P<0.05) was significantly higher than that(51.6%) in HFCS. While, in the presence of 200 M dbcAMP, the maturation promoting activity of HFF (GVBD: 70.5%, $p<10^{-6}$; 1st PB extrusion: 67.1%, $p<10^{-3}$) was significantly greater than that of HFCS(GVBD: 35.2%; 1st PB extrusion: 41.1%). The oocytes cultured in the fraction of HFF containing high M.W. components showed higher meiotic maturation rates than the oocytes cultured in the low M.W. fraction of HFF. Gonadotropins and $E_2$ were known to improve the completion of maturation changes, and the levels of these hormones were higher in HFF than in HFCS. Therefore, HFF was more effective than HFCS to use for promoting meiotic resumption of mouse oocytes in vitro.

• Korean Institute of Interior Design Journal
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• no.27
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• pp.20-27
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• 2001

The tendency of formalization in Contemporary Architecture deeply relies on the thought of Western Philosophy, which emphasizes the art of image perceived visually but ignores the body and perception of a human beig who uses the architecture and lives in it. On the contrary, Merleau-Ponty asserted long time ago that the world and the body are inseparably related to each other. The phenomenology is important in Architecture, since the center of thought should be taken back to the human body if a artistic meaning can be obtained by Architecture. From this point of view, the meaning of Contemporary Architecture can be renewed by the phenomenological idea of Merleau-Ponty as a means of expanding thought that overcomes the limit of formalization in Contemporary Architecture. This research aims to ream from Steven Holl's work, and show the Architectural elements that are used for preceptual experience of phenomenon and the function of those element sin Architectural space of his works. The result of study on about the phenomenal light and water in Architectural space of Steven Holl is as follows; First, in perceptional experience of phenomenon, time is an important element, which is successive and make a field that cause perceptional experience. Second, light, as a phenomenological element, acted as a means of expressing the comparison and change of light and shadow in Architectural space and showing the change of color by the diversity of time in inner space. Third, water, as a phenomenal lens, not only acted functional but also functioned as an element of sensual experience in Architectural space. It acted as an image containing time, space, just like a mirror that reflect the environment.

• International Journal of Human Ecology
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• v.15 no.2
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• pp.33-41
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• 2014

The bra is one of the most essential pieces of women's underwear which gives support for the breasts and holds them in position to make better outer appearance of upper body. Despite being worn by most women of all ages, brassiere manufacturers often use brassiere sizes and shapes that are close to the body measurements of women in their 20s, which raises questions whether the bras are suitable for older women's physique. Also, many lingerie markets tend to center on designs and tastes of younger women, leaving those of older women are largely ignored in the manufacturing process despite the fact that their purchasing power and market shares are expected to grow in conjunction with the aging of the global population. Against this backdrop, this study attempts to provide information about bra preferences and breast perceptions of the middle-aged and older US women over the age of 40 years to help manufacturers better understand the bra purchasing priorities and bra elements of older women and prompt them to design bras with a high level of fit and comfort that are appreciated by the population groups as a means to ultimately acquire competitive edge in the fast growing aging market. An email survey and interviews were conducted with 301 US women who live in New York City. The findings of this study suggest that there are internal and external factors such as changes in body and breast shapes with age influence the level of fitness and effectiveness of bra components, and thus the responses to the questions on bra preferences and breast perceptions varied between the researched age groups.