• 대한의용생체공학회:의공학회지
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• 제28권5호
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• pp.628-635
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• 2007

We studied the effect of vibratory stimulations of different leg muscles, tibialis anterior(TA) and triceps surae(TS), and plantar zones in ten healthy subjects during 1) quiet standing, 2) forward lean of body, 3) backward lean of body, 4) right lean of body, and 5) left lean of body. The experiments were performed on the force platform. The effect of vibration were measured by monitoring the area of COP(Center of pressure) sway. The subjects wore a vibratory stimulation system on foot and ankles and were given the instruction not to resist against the applied perturbations. The results show that all vibratory stimulations to lower limb muscles and plantar zones reduced the COP sway area. This reduction of the COP sway area occurred also in partial vibratory stimulations during quiet standing. In forward lean of body, vibratory stimulations to TA reduced the COP sway area. During backward lean of body, vibratory stimulations to TS reduced the COP sway area. When the subject was tilted right, vibratory stimulations to left plantar zone reduced the COP sway area. During left lean of body, vibratory stimulations to right plantar zone reduced the COP sway area. Thus, the influence of vibratory stimulations to leg muscle and plantar zones differed significantly depending on the lean of body. We suggest that the vibration stimuli from leg muscles and plantar zones could be selectively used to help maintaining postural balance stable.

• 한국의상디자인학회지
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• 제14권1호
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• pp.81-96
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• 2012

The purpose of this study is to provide fundamental information for standardization of 3D body measurement. This research analyzes errors occurring in the process of extracting body size from 3D body scan data. First, as a result of analyzing basic state of the 3D body scanner's calibration, the point number of each section was almost the same, while the right and left as well as the front and back coordinates of the center of gravity are not, showing unstable data. Nevertheless, the latter does not influence on the size of cylinder such as width and circumference. Next, we analyzed point coordinates variations of scan data on a mannequin nude by life casting. The result was great deflection in case of complicated or horizontal sections including the reference point beyond proper distance from centers of four cameras. In case of the mannequin's size, accuracy proves comparatively high in that measurement errors in height, width, depth, and length dimension occurred all within allowable errors, only except chest depth, while there were a lot of measurement errors in a circumference dimension. Secondly, analysis of accuracy of automatic extraction identification program algorithm presented that a semi-automatic measurement program is better than an automatic measurement program. While both of them ate very acute in parts related to crotch, they are not in armpit related parts. Therefore, in extracting of human body size from 3D scan data, what really matters seems to parts related to armpits.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.999-1010
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• 2021

Polycyclic aromatic hydrocarbons (PAHs) are ubiquitous in the environment. They are highly toxigenic and carcinogenic. Probiotic bacteria isolated from fermented foods were tested to check their ability to degrade and/or detoxify PAHs. Five probiotic bacteria with distinct morphologies were isolated from a mixture of 26 fermented foods co-cultured with benzo(a)pyrene (BaP) containing Bushnell Haas minimal broth. Among them, B. velezensis (PMC10) significantly reduced the abundance of BaP in the broth. PMC10 completely degraded BaP presented at a lower concentration in broth culture. B. velezensis also showed a clear zone of degradation on a BaP-coated Bushnell Haas agar plate. Gene expression profiling showed significant increases of PAH ring-hydroxylating dioxygenases and 4-hydroxybenzoate 3-monooxygenase genes in B. velezensis in response to BaP treatment. In addtion, both live and heat-killed B. velezensis removed BaP and naphthalene (Nap) from phosphate buffer solution. Live B. velezensis did not show any cytotoxicity to macrophage or human dermal fibroblast cells. Live-cell and cell-free supernatant of B. velezensis showed potential anti-inflammatory effects. Cell-free supernatant and extract of B. velezensis also showed free radical scavenging effects. These results highlight the prospective ability of B. velezensis to biodegrade and remove toxic PAHs from the human body and suggest that the biodegradation of BaP might be regulated by ring-hydroxylating dioxygenase-initiated metabolic pathway.

• Korean Journal of Acupuncture
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• 제36권4호
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• pp.292-299
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• 2019

Objectives : DongUiBoGam is one of the representative medical literatures in Korea. We used text mining methods and analyzed the characteristics of the indications of each meridian in the second chapter of DongUiBoGam, WaeHyeong, which addresses external body elements. We also visualized the relationships between the meridians and the disease sites. Methods : Using the term frequency-inverse document frequency (TF-IDF) method, we quantified values regarding the indications of each meridian according to the frequency of the occurrences of 14 meridians and 14 disease sites. The spatial patterns of the indications of each meridian were visualized on a human body template according to the TF-IDF values. Using hierarchical clustering methods, twelve meridians were clustered into four groups based on the TF-IDF distributions of each meridian. Results : TF-IDF values of each meridian showed different constellation patterns at different disease sites. The spatial patterns of the indications of each meridian were similar to the route of the corresponding meridian. Conclusions : The present study identified spatial patterns between meridians and disease sites. These findings suggest that the constellations of the indications of meridians are primarily associated with the lines of the meridian system. We strongly believe that these findings will further the current understanding of indications of acupoints and meridians.

• Journal of Microbiology and Biotechnology
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• 제32권2호
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• pp.238-247
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• 2022

Since the skin covers most surfaces of the body, it is susceptible to damage, which can be fatal depending on the degree of injury to the skin because it defends against external attack and protects internal structures. Various types of artificial skin are being studied for transplantation to repair damaged skin, and recently, the production of replaceable skin using three-dimensional (3D) bioprinting technology has also been investigated. In this study, skin tissue was produced using a 3D bioprinter with human skin cell lines and cells extracted from mouse skin, and the printing conditions were optimized. Gelatin was used as a bioink, and fibrinogen and alginate were used for tissue hardening after printing. Printed skin tissue maintained a survival rate of 90% or more when cultured for 14 days. Culture conditions were established using 8 mM calcium chloride treatment and the skin tissue was exposed to air to optimize epidermal cell differentiation. The skin tissue was cultured for 14 days after differentiation induction by this optimized culture method, and immunofluorescent staining was performed using epidermal cell differentiation markers to investigate whether the epidermal cells had differentiated. After differentiation, loricrin, which is normally found in terminally differentiated epidermal cells, was observed in the cells at the tip of the epidermal layer, and cytokeratin 14 was expressed in the lower cells of the epidermis layer. Collectively, this study may provide optimized conditions for bioprinting and keratinization for three-dimensional skin production.

• 대한간호학회지
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• 제29권6호
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• pp.1244-1253
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• 1999

The purpose of this study was to explore and describe the experience of Dan-Jeon breathing training and of Qi as a essential substance in forming human body. The sample consists of 7 participants who are Dan-Jeon Breathing training in a Training center, Pusan, Korea. They were asked open-ended questions in order for them to talk about their experiences. With permission of the subjects, the interviews were recorded and transcribed. The summarized results of this research are following. 1. The purpose of Dan-Jeon Breathing The interview data was organized by themes into 4 categories : hope for health recovery, a concern about Dan-Jeon Breathing, seeking meaning of life, change of lifestyle 2. The experience of Qi during Dan-Jeon Breathing training The interview data was organized by themes into 3 categories : an autonomic movement of body, spiritual experience, conviction of existence of Qi. 3. The change after Dan-Jeon Breathing training. The interview data was organized by themes into 7 categories : physical health promotion, emotional relaxation, promoting brain function, positive attitude about life, love to others, investigation for self, improvement on Qi feeling..

• 한국발생생물학회지:발생과생식
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• 제19권4호
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• pp.189-196
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• 2015

This study was examined the ovogenesis of Ussurian bullhead, Leiocassis ussuriensis and the morphological development of its larvae and juveniles and to use the results as basic information for the preservation of species and resource enhancement. For artificial egg collection, human chorionic gonadotropin (HCG) was injected at a rate of 10 IU per gram of fish weight. During breeding period, water temperature maintained at $24.5{\sim}26.5^{\circ}C$ (mean $25.0{\pm}0.05^{\circ}C$). The process of ovogenesis reached the two-cell stage in 50 minutes after fertilization. In 73 hours of fertilization the movement of the embryoid body became active state and the larvae began to hatch from the tail through the oolemma. Length of prelarvae were 6.33~6.50 mm long (mean $6.40{\pm}0.06mm$) just after hatching having yolk with their mouth not opened. After thirty eight days of hatching, juveniles were 30.6~32.5 mm long (mean $31.5{\pm}0.65mm$). The color was dark yellowish brown throughout the entire body, and the number of caudal fin rays developed to thirty six perfectly.

• International Journal of Stem Cells
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• 제16권2호
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• pp.145-155
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• 2023

Background and Objectives: Embryologically, mesodermal development is closely related to the development of various organs such as muscles, blood vessels, and hearts, which are the main organs that make up the body. However, treatment for mesoderm developmental disorders caused by congenital or acquired factors has so far relied on surgery and drug treatment for symptom relief, and more fundamentally, treatment for mesoderm developmental disorders is needed. Methods and Results: In our study, microRNA (miRNA), which plays an important role in the mesoderm development process, was identified and the developmental function was evaluated. miRNAs consist of small nucleotides, which act as transcription factors that bind to the 3' untranslated region and suppressed target gene expression. We constructed the human embryonic stem cell (hESC) knockout cell line and analyzed the function and characteristics of miR-5739, which plays an important role in mesoderm lineage. miR-5739 acts as a transcription factor targeting SMA, Brachyury T, Hand1, which controls muscle proliferation and differentiation, and KDR gene, which regulates vessel formation in vitro. In vivo results suggest a role in regulating muscle proliferation and differentiation. Gene ontology analysis confirmed that the miR-5739 is closely related to genes that regulate muscle and vessel proliferation and differentiation. Importantly, abnormal expression of miR-5739 was detected in somatic cells derived from patients with congenital muscle disease. Conclusions: Our study demonstrate that miR-5739 gene function significantly affects transcriptional circuits that regulate muscle and vascular differentiation during embryonic development.

• 한국소음진동공학회논문집
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• 제18권9호
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• pp.905-912
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• 2008

Notion sickness is well known to be caused by long time exposure to the very low frequency motion in the multiple axes of human body Since the vestibular system for the perception of low frequency motion is located in the head, accurate measurement of 6 degree of freedom head motion is of great importance. In this study, the measurement system consisting of a safety helmet and 9 translational accelerometers was constructed for the estimation of 3 translational and 3 rotational motions of human head. Since estimation errors of 3 rotational components can be significantly magnified even by small offset of the sensitivity axis from the geometric center of an accelerometer, accurate measurement of sensitivity axis must be preceded. The method for accurate estimation of the offset was proposed, and the effect of offset on the estimation of angular acceleration was investigated.

• Journal of Ginseng Research
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• 제44권2호
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• pp.229-237
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• 2020

Background: We investigated the tolerability and pharmacokinetic properties of various ginsenosides, including Rb1, Rb2, Rc, Rd, and compound K, after single or multiple administrations of red ginseng extract in human beings. Methods: Red ginseng extract (dried ginseng > 60%) was administered once and repeatedly for 15 days to 15 healthy Korean people. After single and repeated administration of red ginsengextract, blood sample collection, measurement of blood pressure and body temperature, and routine laboratory test were conducted over 48-h test periods. Results: Repeated administration of high-dose red ginseng for 15 days was well tolerated and did not produce significant changes in body temperature or blood pressure. The plasma concentrations of Rb1, Rb2, and Rc were stable and showed similar area under the plasma concentration-time curve (AUC) values after 15 days of repeated administration. Their AUC values after repeated administration of red ginseng extract for 15 days accumulated 4.5- to 6.7-fold compared with single-dose AUC. However, the plasma concentrations of Rd and compound K showed large interindividual variations but correlated well between AUC of Rd and compound K. Compound K did not accumulate after 15 days of repeated administration of red ginseng extract. Conclusion: A good correlation between the AUC values of Rd and compound K might be the result of intestinal biotransformation of Rb1, Rb2, and Rc to Rd and subsequently to compound K, rather than the intestinal permeability of these ginsenosides. A strategy to increase biotransformation or reduce metabolic intersubject variability may increase the plasma concentrations of Rd and compound K.