• Korean Journal of Animal Reproduction
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• v.26 no.2
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• pp.87-94
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• 2002

This study was performed during the four seasons for the production of transgenic pigs containing the Cellulase Digest Gene. Purebred Landrace gilts and sows approximately 8∼15 months of age (n=126) were used for the collection of 1-cell zygotes for DNA microinjection and transfer. Retrospectively, estrus synchronization and superovulation schemes were evaluated to assess practicality fur zygote collection. Synchronization and superovulation procedures were used that cyclic gilts were synchronized with 20mg altrenogest (ALT) per day for 9 days after PG600 administration followed by superovulation with 1000 IU pregnant mares serum gonadotropin (PMSG) and 750IU human chorionic gonadotrophin (hCG). The cellulase digestion gene for microinjection is rat elasterase promoter (rEl) linked to CelD gene. After hormone treatment, 1,422 embryos were collected from 91 donors and 95.6% (1,359/1,422) embryos were in 1-cell stage which can be visualized the pronuclei for DNA microinjection. A total of 725 DNA microinjected embryos transferred into 35 recipients and produced 65 piglets from 13 litters. Pregnancy rate according to the number of transferred embryos to recipients was higher the group which received 21 to 24 embryos (50.0%) than other groups 20.0% in less and 33.3% in more. A tail tissue was collected from 65 piglets for biopsy. PCR screening was performed on each DNA sample using two separate sets of primers specific for the 5'- and 3'-flanking region of the rEl-CelD gene. Five of the 65 piglets (7.69%) were positive for the transgene. This study provide useful information regarding production of transgenic pig for bioreactor research.

• Journal of Ginseng Research
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• v.10 no.1
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• pp.1-10
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• 1986

To evaluate the possible utilization of ginseng by-products, chemical components of ginseng residue, reducing ability of DPPH, effect of residue extract on the yeast growth, amino acid contents of yeast cell, increase of residue extract yield by enzyme treatment were studied. Alcohol and water extract residue contained 43-46% total reducing sugar and 14-15% crude protein, while alcohol extract residue had 0.18% n-BuOH extract. Water extract of alcohol extract residue had about 45% reducing ability of DPPH in comparison with that of alcohol extract from ginseng roots. Essential nutrients for the yeast growth were found in extract when Saccharomyces cerevisiae was cultured in Czapeck medium, a compound medium, with the residue. The addition of residue extract to malt medium, a natural medium, enhanced 30-40% yeast growth. And content of each amino acid in yeast cell cultured on malt medium with ginseng residue extract was much more than that of the cell cultured without ginseng extract, but amino acid composition of yeast cell did not differ from one another. The treatment of alcohol extract residue with cellulase increased 250% yield of residue extract.

• Horticultural Science & Technology
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• v.19 no.4
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• pp.545-549
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• 2001

The relationship between ethylene action and flesh softening in post-storage persimmon fruits was investigated by treating the fruits with 1-MCP. The patterns of firmness change caused by various treatments with 1-MCP and ethylene were similar to those of changes in cell wall-degrading enzyme activities, including cellulase, PG, PME, and ${\beta}$-galactosidase. Moreover, the activities of these enzymes were inhibited by 1-MCP treatment. These results show that the cell wall-degrading enzymes influenced by ethylene account for the flesh softening process of post-storage persimmon fruit. The ethylene production of fruits, as measured by ACC content and ACC oxidase activity, nevertheless, was not influenced by 1-MCP treatments. It is suggested that the flesh softening phenomena in post-storage persimmon fruits is correlated to the ethylene responsiveness of tissue rather than the ethylene production rate per se.

• Journal of Animal Environmental Science
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• v.2 no.2
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• pp.135-145
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• 1996

This study was carried out to investigate changes of physico-chemical properties and microbial activity during the early stage of composting with pig and chicken manure. The results were as follows; 1. The temperature was rapidly increased from the 3rd to the 7th day, and especially the pig manure compost preparing with enzyme was maintained $56^{\circ}C{\sim}69^{\circ}C$. 2. The pH range was shown $7.7{\sim}9.3$, and the pH level increased from the 3rd day to 25th day. Also after the 25th day the pH level decreased gradually. 3. The C/N ratio in the pig manure compost decreased 16.8 at the 30th day, while the compost containing enzymes decreased 19.2 at the 30th day. Chicken manure compost showed similar results at the 28 of C/N ratio at the 30th day with enzyme treatment. 4. The total ammount of sugar in pig manure compost was $6,000{\sim}7,000mg/kg$, while the chicken manure compost was $2,000{\sim}4,000mg/kg$. However, there was no significant difference in view point of enzyme treatment. 5. Cellulase, phosphatase and xylanase activity were continually increased, however amylase and urease activity were not changed during composting.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.11
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• pp.1604-1609
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• 2008

A series of in vitro and in vivo experiments were conducted to investigate the effects of the mixture of Tween 80 and cellulolytic enzymes (xylanase and cellulase) on total tract nutrient digestibility and rumen cellulolytic bacterial adhesion rates in Holstein steers. Ground timothy hay sprayed with various levels of Tween 80 and cellulolytic enzymes was used as substrates in an in vitro experiment to find out the best combinations for DM degradation. The application level of 2.5% (v/w) Tween 80 and the combination of 5 U xylanase and 2.5 U cellulase per gram of ground timothy hay (DM basis) resulted in the highest in vitro dry matter degradation rate (p<0.05). Feeding the same timothy hay to Holstein steers also improved in vivo nutrient (DM, CP, CF, NDF and ADF) digesibilities compared to non-treated hay (p<0.05). Moreover, Tween 80 and enzyme combination treatment increased total ruminal VFA and concentrations of propionic acid and isovaleric acid with decreased acetate to propionate ratio (p<0.001). However, adhesion rates of Fibrobacter succinogenes and Ruminococcus flavefaciens determined by Real Time PCR were not influenced by the treatment while that of Ruminococcus albus was decreased (p<0.05). The present results indicate that a mixture of Tween 80 and cellulolytic enzymes can improve rumen environment and feed digestibility with variable influence on cellulolytic bacterial adhesion on feed.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.11
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• pp.1747-1755
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• 2018

Objective: Common reed (Phragmites communis Trin.) could potentially provide an alternative resource for silage; however, its silage quality is poor. The aim of this study was to investigate the factors in reed that contribute to poor quality and determine how the use of additives at ensiling could improve fermentation quality. Methods: In Experiment 1, we determined the chemical composition and the presence of indigenous lactic acid bacteria (LAB) in reed. We further examined fermentation quality of reed silage under conditions without additives (NA) and treated glucose (G), lactic acid bacteria (L), and their combination (G+L). In Experiment 2, silage of NA, and with an addition of cellulase and lactic acid bacteria (CL) were prepared from harvested reed. The harvested reeds were fertilized at nitrogen concentrations of 0, 4, 8, and $12g\;N/m^2$ and were harvested thrice within one year. Results: The indigenous LAB and fermentable carbohydrates are at extremely low concentrations in reed. Reed silage, to which we added G+L, provided the highest quality silage among treatments in Experiment 1. In Experiment 2, N fertilization had no negative effect on silage quality of reed. The harvest times decreased fermentable carbohydrate content in reed. The CL treatment provided a higher lactic acid content compared to the NA treatment. However, the quality of CL treated silage at the second and third harvests was significantly lower than at the first harvest, due to a reduction in carbohydrates caused by frequent harvesting. Conclusion: The causes of poor quality in reed silage are its lack of indigenous LAB and fermentable carbohydrates and its high moisture content. In addition, reed managed by frequent harvesting reduces carbohydrate content. Although the silage quality could be improved by adding CL, higher-quality silage could be prepared by adding fermentable carbohydrates, such as glucose (rather than adding cellulases).

• Microbiology and Biotechnology Letters
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• v.9 no.3
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• pp.129-137
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• 1981

The purpose of this study was to extract saponin efficiently from ginseng leaves and peelings by macerating them with microbial enzyme. To begin with, we selected G-211 strain having the highest macerating activity among several rotting molds of fresh ginseng. Crude macerating enzyme was prepared from this G-2l1 strain by ammonium sulfate precipitation, and was applied to macerating leaves and peelings of ginseng. The optimal pH of the enzyme for maceration was 5.0 in both leaves and peelings of ginsen g. The optimal pH for the extraction of soluble matters and saponins was 4.5 and 5.5 in ginseng leaves and ginseng peelings, respectively. When this enzyme was treated together with crude cellulase from Trichoderma viride (To4), the extract content of saponin was 3.45% for ginseng leaves and 3.90% for ginseng peelings. Their yields were 39.8 % and 39.3 % of total saponin amounts in ginseng leaves and ginseng peelings, respectively. The ginsenoside patterns of saponins extracted with the treatment of enzymes were also studied by HPLC technics.

• Journal of The Korean Society of Grassland and Forage Science
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• v.8 no.3
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• pp.169-174
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• 1988

An experiment was conducted to determine the feeding value of Arundinella hirat silage making by different methods ie., added starch 3%, formic acid 1% (FA), anhydrous ammonia ($NH_3$) 3%, and dried poultry waste (DPW) 3%. The samples were analyzed organic acid, cell wall constituent (NDF), cell contents (NDS), lignincutin-silica (ADL), hemicellulose (H: CHO) and in vitro dry matter digestibility (DMD) by pepsin-cellulase technique. The feeding value of silages were compared with corn siiage. The results are summarized as follows; 1.Formic acid treated to silage was lower pH(4.0), but anhydrous ammonia (8.37) and DPW added silage (8.72) were higher than that of the other treatment silages. 2.Fermentative quality of corn silage, starch, DPW, $NH_3$, control and FA treated silage were marked 100,81, 77,63,62 and 58, respectively. 3.Silage with $NH_3$, (23.57%) and DPW (10.42%) of content of protein were higher than that of other treated silages. 4.Silage with $NH_3$, was significantly lower contents of NDF, but did not ADF of among the treated silages. 5.In the contents of ADL, starch added silage was significantly decreased (p < 0.05), but did not the other treatment. 6.DMD of in vitro by pepsin-cellulase of silage with starch was increased three folds as equal to corn silage but did not increased the other treated silages. 7.Correlationship of latic acid an total acid, and NDS were a positive but lactic acid and NDF was a negative correlation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.13 no.3
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• pp.170-176
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• 1993

The yield, viability and continuous culture of isolated Italian ryegrass protoplasts were investigated. The effects of cold treatment (4^{\circ}C.$) for 7 days and basic LS medium supplemented with 5mg/l $AgNO_3$ showed effectively on embryogenic callus induction and regeneration responses of immature and mature embryos or young inflorescences subcultured every 4 weeks on basic medium. The optimum combinations of growth regulator on the regeneration responses was 0.2mg/l BAP and 2mg/l 2, 4-D. Calli induced inflorescences were suspended in its liquid medium for 5 days before enzyme treatment. Maximum protoplast yield and viability were obtained after digestion in enzyme solution contained 4% cellulase R10. 2% macerozyme and 2% pectinase in 0.6M mannitol. Cell division and microcalli development were observed in isolated protoplasts cultured in agarose culture of KM8P medium.

• The Research Journal of the Costume Culture
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• v.12 no.5
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• pp.737-742
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• 2004

The purpose of this study is restoration for tearing strength of the durable press (DP) finished 100% cotton fabric by enzymatic treatment. Dimethylol Dihydroxy Ethylene Urea (DMDHEU) was used as a DP finish chemical. Enzymes (cellulase, pectinase, protease, lipolase) were selected based on their specific reaction activities. Ideal application of the enzymes for this work was to remove cross-links created by DMDHEU on the surface of the fibers to offer migration property between microstructures of cellulose, yet cross-links that exist inside of the fibers are still remained to impart effect of wrinkle resistance. Physical characteristics (tearing strength, wrinkle recovery, FT-IR) of enzyme treated samples were measured and compared. It was found out that, in case of enzyme treatment, most of enzymes didn't have a great effect on tearing strength, but, in case of Protease, tearing strength increased at DMDHEU 2% treatment. As a result of an experiment on wrinkle recovery of the textiles treated with enzyme making density of DMDHEU different whenever respective experiment was made, it was discovered that density of DMDHEU increased as wrinkle recovery increased and, in the relation to enzyme treatment especially in Lipase enzyme treatment, the lesser density of DMDHEU, the more wrinkle recovery increased.