• Asian-Australasian Journal of Animal Sciences
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• v.20 no.5
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• pp.802-810
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• 2007

Fibrobacter succinogenes, a Gram-negative, anaerobic ruminal bacterium is a major fibre digesting species in the rumen. It intensively degrades plant cell walls by an erosion type of mechanism, burrowing its way through the complex matrix of cellulose and hemicellulose with the release of digestible and undigested cell wall fragments. The enzymes involved in this process include a combination of glucanases, xylanases, arabinofuranosidase(s) and esterases. The genome of the bacterium has been sequenced and this has revealed in excess of 100 putative glycosyl hydrolase, pectate lyase and carbohydrate esterase genes, which is greater than the numbers reported present in other major cellulolytic organisms for which genomes have been sequenced. Modelling of the amino acid sequences of two glycanases, CedA and EGB, by reference to crystallized homologs has enabled prediction of the major features of their tertiary structures. Two dimensional gel electrophoresis in conjunction with mass spectroscopy has permitted the documentation of proteins over expressed in F. succinogenes grown on cellulose, and analysis of the cell surfaces of mutant strains unable to bind to cellulose has enabled the identification of candidate proteins with roles in adhesion to the plant cell wall substrate, the precursor to cellulose biodegradation.

• Journal of Korea Foundry Society
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• v.27 no.6
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• pp.237-242
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• 2007

To improve the quality of the product and the cost efficiency, the joining of A356 alloy to an Al-18wt%Si alloys has been performed by centrifugal casting. The influence of the mold preheating temperature, the pouring temperature and the rotational velocity of the mold on the microstructures of the shell in the centrifugal casting was investigated using the experimental and simulation methods. In the present study, the cellular automaton (CA) technique and the finite volume method (FVM) were adopted to simulate the evolution of the macro structures and to calculate the temperature profiles, respectively. The evolution of the microstructures was also simulated using a modified cellular automaton (MCA) model. The optimal rotational speed of the mold for obtaining the sound shape of the shell was estimated experimentally to be over 1200 rpm. For the uniform microstructure, the outer shell needs to be cast with higher preheated mold temperature and lower pouring temperature, and the melt was poured at lower temperature in the inner shell. In order to obtain the sound shape of the joining, the different materials were poured simultaneously.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.32 no.8C
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• pp.689-695
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• 2007

Wireline relay stations(RS's) are connected to cellular base stations(BS's) via radio-over-fiber(RoF) to enhance system capacity and to reduce shadow areas. Unlike wireless multi-hop systems, BS-to-RS signaling is transmitted out-of-band, thus reducing the effect of interference caused by frequency reuse. In this paper, antenna arrays used in addition to the wireline RS's are considered to evaluate the transmission capacity gain and performance variations according to the may structures. In particular, RS locations to maximize the gain, may distribution patterns for a given number of antenna elements, performance enhancement for a varying number of elements are experimentally determined to suggest a proper utilization of antenna ways in conjunction with wireline RS's.

• Journal of Welding and Joining
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• v.19 no.4
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• pp.429-436
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• 2001

The effect of welding condition on the microstructures of the weld metal in A7N01 welded by $CO_2$ laser was investigated. The number of ripples was increased with decreasing power and increasing welding speed. In the bead without ripple lines, the subgrain microstructures distribution from the fusion line toward the center of the bead were in the order of cellular, dendritic and equiaxed dendrite. However, in the bead with ripple lines, cellular and dendritic were formed between the fusion boundary and the ripple line. Inaddition, those structures were also observed between the ripple line. Equiaxed dendrites were formed only at the center line region. Cellular and dendritics formed near the ripple line were larger than those formed near the fusion boundary. The cooling rates estimated by the dendrite arm spacing were in the range of 200 to 1150oC/s. Cooling rate was increased with decreasing the power and increasing the welding speed. Mg and Zn segregated at the boundaries of cellulars and dendritics, Mg was segregated more than Zn. The segregation of Mg and Zn decreased with increasing cooling rate. Hardness of the weld metal was lower than that of the base metal in all welding conditions and increased as the cooling rate increased.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.3
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• pp.493-499
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• 2008

Cellular Automata(CA) has been used as modeling and computing paradigm for a long time. And CA has been used to model many physical systems. While studying the models of such systems, it is seen that as the complexity of the physical system increase, the CA based model becomes very complex and becomes to difficult to track analytically. Also such models fail to recognize the presence of inherent hierarchical nature of a physical system. In this paper we give the characterization of Hierarchical Cellular Automata(HCA). Especially we analyze transition rules, characteristic polynomials and cyclic structures of HCA.

• Structural Engineering and Mechanics
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• v.71 no.3
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• pp.283-290
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• 2019

In global competition manufacturing companies have to produce modern, new constructions from advanced materials in order to increase competitiveness. The aim of my research was to develop a new composite cellular plate structure, which can be primarily used for structural elements of road, rail, water and air transport vehicles (e.g. vehicle bodies, ship floors). The new structure is novel and innovative, because all materials of the components of the newly developed structure are composites (laminated Carbon Fiber Reinforced Plastic (CFRP) deck plates with pultruded Glass Fiber Reinforced Plastic (GFRP) stiffeners), furthermore combines the characteristics of sandwich and cellular plate structures. The material of the structure is much more advantageous than traditional steel materials, due mainly to its low density, resulting in weight savings, causing lower fuel consumption and less environmental damage. In the study the optimal construction of a given geometry of a structural element of a road truck trailer body was defined by single- and multi-objective optimization (minimal cost and weight). During the single-objective optimization the Flexible Tolerance Optimization method, while during the multi-objective optimization the Particle Swarm Optimization method were used. Seven design constraints were considered: maximum deflection of the structure, buckling of the composite plates, buckling of the stiffeners, stress in the composite plates, stress in the stiffeners, eigenfrequency of the structure, size constraint for design variables. It was confirmed that the developed structure can be used principally as structural elements of transport vehicles and unit load devices (containers) and can be applied also in building construction.

• Proceedings of the Korean Vacuum Society Conference
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• 2011.02a
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• pp.7-7
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• 2011

Graphene and graphene derivatives have attracted enormous attention from various research fields for applications in electronic devices, transparent electrodes, biosensors, drug delivery system and surface coatings. In the viewpoint of chemist, the chemical structure of graphene derivatives seems intriguing but detailed structures are being revealed only recently while engineering approaches for various applications are being executed very actively. In addition, cytotoxicity and mammalian cellular responses to graphene have not thoroughly investigated yet in spite of the importance in bio-applications and environment. In this talk, I'll introduce recent studies which report cytotoxicity and behaviors of mammalian cells when the cells are exposed to graphene (as well as some bio-applications of graphene), especially to get closer to answers to these questions, "how we understand and how/why we use graphene in biotechnology".

• Journal of Microbiology and Biotechnology
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• v.20 no.12
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• pp.1647-1652
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• 2010

Autophagy refers to the bulk degradation of cellular proteins and organelles through an autophagosome and plays a pivotal role in the development, cellular differentiation, aging, and elimination of aberrant structures. A failure of autophagy has been implicated in a growing list of mammalian disease states, including cancer and cardiomyopathy. Two ubiquitin-like systems are highly involved in autophagy, especially in the formation of autophagosomes. Here, we purified and characterized Atg7 (an E1-like enzyme), and Atg3 and Atg10 (E2-like enzymes) in order to gain an insight into the role played by ubiquitin-like systems in the formation of autophagosomes. Interestingly, we observed that Atg7 forms a homodimer to construct an active conformation, unlike other E1-like enzymes. Although Atg3 was detected as a monomer under physiological conditions, Atg10 existed in an oligomeric form, indicating that the mechanism by which Atg10 functions may differ from that of Atg3.

• Molecules and Cells
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• v.41 no.7
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• pp.622-630
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• 2018

Leukocyte common antigen-related protein tyrosine phosphatases (LAR-RPTPs) are cellular receptors of heparan sulfate (HS) and chondroitin sulfate (CS) proteoglycans that regulate neurite outgrowth and neuronal regeneration. LAR-RPTPs have also received particular attention as the major presynaptic hubs for synapse organization through selective binding to numerous postsynaptic adhesion partners. Recent structural studies on LAR-RPTP-mediated trans-synaptic adhesion complexes have provided significant insight into the molecular basis of their specific interactions, the key codes for their selective binding, as well as the higher-order clustering of LAR-RPTPs necessary for synaptogenic activity. In this review, we summarize the structures of LAR-RPTPs in complex with various postsynaptic adhesion partners and discuss the molecular mechanisms underlying LAR-RPTP-mediated synaptogenesis.

• The Korean Journal of Nuclear Medicine
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• v.36 no.4
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• pp.209-223
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• 2002

By considering the biological properties of a tumor, it should be possible to realize better results in cancer therapy. PET imaging offers the opportunity to measure tumor growth non-invasively and repeatedly as an early assessment of response to cancer therapy. Measuring cellular growth instead of energy metabolism showed offer significant advantages in evaluating therapy. Thymidine and its derivative nucleoside compounds can be changed to mono, di- and tri- phosphate compounds by thymidine kinase and then be incorporated into DNA. Their bindings are increased in highly proliferating cells due to the high DNA synthesis rate. To evaluate cell proliferation, many kinds of thymidine and uridine derivatives have been labeled with positron emitter and radioactive iodine. Compared to radiopharmaceuticals which have radioisotope labeled base ring such as pyirmidine, the radiopharmacuticals which have radioisotope labeled sugar ring are more stable in vivo and have metabolic resistance. The biological properties such as DNA incorporation ratios are highly dependent on their chemical structures and metabolic processes. This overview describes synthesis of radiopharmaceuticals and their biological properties for imaging of tumor cell proliferation.