• 한국정보기술학회 영문논문지
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• 제10권1호
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• pp.25-36
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• 2020

Motivation: Fibers as the extracellular filamentous structures determine the shape of the cytoskeletal structures. Their characterization and reconstruction from a 3D cellular image represent very useful quantitative information at the cellular level. In this paper, we presented a novel automatic method to extract fiber diameter distribution through a pipeline to reconstruct fibers from 3D fluorescence confocal images. The pipeline is composed of four steps: segmentation, skeletonization, template fitting and fiber tracking. Segmentation of fiber is achieved by defining an energy based on tensor voting framework. After skeletonizing segmented fibers, we fit a template for each seed point. Then, the fiber tracking step reconstructs fibers by finding the best match of the next fiber segment from the previous template. Thus, we define a fiber as a set of templates, based on which we calculate a diameter distribution of fibers.

• Steel and Composite Structures
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• 제46권5호
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• pp.709-718
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• 2023

The absence of an important portion of the web plate in steel beams with multiple circular perforations, cellular beams, causes the web plate to undergo distortions prior to and during lateral torsional buckling (LTB). The conventional LTB equations in the codes and literature underestimate the buckling moments of cellular beams due to web distortions. The present study is an attempt to develop analytical methods for estimating the elastic buckling moments of cellular beams. The proposed methods rely on the reductions in the torsional and warping rigidities of the beams due to web distortions and the reductions in the weak-axis bending and torsional rigidities due to the presence of web openings. To test the accuracy of the analytical estimates from proposed solutions, a total of 114 finite element analyses were conducted for six different standard IPEO sections and varying unbraced lengths within the elastic limits. These analyses clearly indicated that the LTB solutions in the AISC 360-16 and AS4100:2020 codes overestimate the buckling loads of cellular beams within elastic limits, particularly at shorter span lengths. The LDB solutions in the literature and the Eurocode 3 LTB solution, on the other hand, provided conservative buckling moment estimates along the entire range of elastic buckling.

• 대한기계학회논문집A
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• 제31권11호
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• pp.1124-1130
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• 2007

This paper presents a new way for fabricating sandwich plates with tetrahedral truss cores. The tetrahedral truss cores are manufactured through metal expanding and bending process and then brazed with solid face sheets. The properties of sandwich plates with the tetrahedral truss cores composed of a wrought steel SS41 under compression and shear loading have been investigated. Good agreement is observed between the measured and predicted peak strengths. Comparisons with normalized compressive strength for other cellular metals have indicated that the tetrahedral truss structures outperform aluminum open cell forms and woven core sandwich plates.

• BMB Reports
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• 제42권3호
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• pp.131-135
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• 2009

MicroRNAs control gene expression by inhibiting translation or promoting degradation of their target mRNAs. Since the discovery of the first microRNAs, lin-4 and let-7, in C. elegans, hundreds of microRNAs have been identified as key regulators of cell fate determination, lifespan, and cancer in species ranging from plants to humans. However, while microRNAs have been shown to be particularly abundant in the brain, their role in the development and activity of the nervous system is still largely unknown. In this review, we describe recent advances in our understanding of microRNA function at synapses, the specialized structures required for communication between neurons and their targets. We also propose how these advances might inform the molecular model of memory.

• 대한전기학회:학술대회논문집
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• 대한전기학회 1998년도 하계학술대회 논문집 G
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• pp.2193-2196
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• 1998

Cellular automata are dynamical systems in which space and time are discrete, where each cell has a finite number of states and updates its states by interactive rules among the cell-neighborhood. From the characteristics of self-reproduction and self- organization, it is possible to create a neural network which has the specific patterns or structures dynamically. CAM-Brain is a kind of such neural network system which evolves its structure by adopting evolutionary computations like genetic algorithms (GA). In this paper, we suggest the evolution strategies for the structure of neural networks based on cellular automata.

• Steel and Composite Structures
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• 제18권2호
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• pp.325-344
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• 2015

Experimental tests on cellular beams with sinusoidal openings showed two main failure modes around the openings. They concern the formation of four plastic hinges and the local instability of the sinusoidal part of the opening. In parallel, numerical analysis of the sinusoidal part of the opening revealed the existence of an elastic rotational restraint between the intermediate web-post and the adjacent opening panel. The aim of the present study is to present an approach to quantify this rotational restraint. Through the response surface method, a mathematical model is proposed. It shows a great ability to predict the rotational restraint value as a function of the geometrical parameters of the opening. This model can be used to perform an extensive study with various geometrical configurations of beams with the aim to develop a reliable and realistic analytical model predicting the resistance of the sinusoidal openings.

• Steel and Composite Structures
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• 제44권5호
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• pp.685-690
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• 2022

In this paper, the equivalent material properties of cellular metamaterials with different types of perforations have been presented using finite element (FE) simulation of tensile test in Abaqus commercial software. To this end, a Representative Volume Element (RVE) has been considered for each type of cellular metamaterial with regular array of circular, square, oval and rectangular perforations. Furthermore, both straight and perpendicular patterns of oval and rectangular perforations have been studied. By applying Periodic Boundary conditions (PBC) on the RVE, the actual behavior of cellular material under uniaxial tension has been simulated. Finally, the effective Young's modulus, Poisson's ratio and mass density of various metamaterials have been presented as functions of relative density of the RVE

• BMB Reports
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• 제54권10호
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• pp.489-496
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• 2021

Chromatin has highly organized structures in the nucleus, and these higher-order structures are proposed to regulate gene activities and cellular processes. Sequencing-based techniques, such as Hi-C, and fluorescent in situ hybridization (FISH) have revealed a spatial segregation of active and inactive compartments of chromatin, as well as the non-random positioning of chromosomes in the nucleus, respectively. However, regardless of their efficiency in capturing target genomic sites, these techniques are limited to fixed cells. Since chromatin has dynamic structures, live cell imaging techniques are highlighted for their ability to detect conformational changes in chromatin at a specific time point, or to track various arrangements of chromatin through long-term imaging. Given that the imaging approaches to study live cells are dramatically advanced, we recapitulate methods that are widely used to visualize the dynamics of higher-order chromatin structures.

• Molecules and Cells
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• 제43권11호
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• pp.899-908
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• 2020

Intrinsically disordered proteins or regions (IDPs or IDRs) are widespread in the eukaryotic proteome. Although lacking stable three-dimensional structures in the free forms, IDRs perform critical functions in various cellular processes. Accordingly, mutations and altered expression of IDRs are associated with many pathological conditions. Hence, it is of great importance to understand at the molecular level how IDRs interact with their binding partners. In particular, discovering the unique interaction features of IDRs originating from their dynamic nature may reveal uncharted regulatory mechanisms of specific biological processes. Here we discuss the mechanisms of the macromolecular interactions mediated by IDRs and present the relevant cellular processes including transcription, cell cycle progression, signaling, and nucleocytoplasmic transport. Of special interest is the multivalent binding nature of IDRs driving assembly of multicomponent macromolecular complexes. Integrating the previous theoretical and experimental investigations, we suggest that such IDR-driven multiprotein complexes can function as versatile allosteric switches to process diverse cellular signals. Finally, we discuss the future challenges and potential medical applications of the IDR research.

• BMB Reports
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• 제53권7호
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• pp.357-366
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• 2020

Currently, most biological research relies on conventional experimental techniques that allow only static analyses at certain time points in vitro or ex vivo. However, if one could visualize cellular dynamics in living organisms, that would provide a unique opportunity to study key biological phenomena in vivo. Intravital microscopy (IVM) encompasses diverse optical systems for direct viewing of objects, including biological structures and individual cells in live animals. With the current development of devices and techniques, IVM addresses important questions in various fields of biological and biomedical sciences. In this mini-review, we provide a general introduction to IVM and examples of recent applications in the field of immunology, oncology, and vascular biology. We also introduce an advanced type of IVM, dubbed real-time IVM, equipped with video-rate resonant scanning. Since the realt-ime IVM can render cellular dynamics with high temporal resolution in vivo, it allows visualization and analysis of rapid biological processes.