• 제어로봇시스템학회:학술대회논문집
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• 2001.10a
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• pp.138.6-138
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• 2001

In recent years, as the battery-powered portable systems such as cellular phone, personal digital assistant (PDA) are widely used, power consumption comes to be a top-priority design concerns. Because those embedded systems become more and more complex than ever and they are operated under severe power and energy constrains, long battery lifetime with a limited energy is very critical. Even though there are various levels of energy optimization techniques, system level techniques are mainly focused on, for their stronger impact on power consumption of the overall system than traditional techniques : circuit level, switch level, architecture level, etc. In this technique, operating system (OS) plays the most important role in the system because it controls ...

• Journal of the Korean Institute of Telematics and Electronics A
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• v.33A no.6
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• pp.220-229
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• 1996

We presetn sytem-level simulation methodology as well as environment setup established for CDMA digtial cellular mobile station in an effort to verify CDMA modem ASIC design. To make the system-level simulation feasible, behavioral modeling of a microcontroller was first carried out with VHDL. In addition, models written in C language were also developed to provide ASIC with realistic input data. Finally, the netlist of CDMA modem ASIC was loaded on the a hardware accelerator, which was interfaced with VHDL simulator, and ismulation was performed by excuting the actual CDMA call processing software. Simulation resutls thus obtained were confirmed by comparing them with the emulation resutls from the actual system constructed on hardware modeler. these methods were proved to be effective in both discovering in advance malfunctions when embedded in the system or design errors of ASIC and reducing simulation time by a factor of as much as 20 in case of simulation at gate-level.

• Journal of Genetic Medicine
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• v.3 no.1
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• pp.33-37
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• 1999

Recombinant adenovirus vector systems with strong promoters have been used to achieve high level production of recombinant protein. However, this overexpression system cause some problems such as disturbance of cell physiology and increment of cellular toxicity. Here, we showed a tetracycline-regulated adenovirus expression vector system. Our results showed that the expression level of transgene(p-53) was high and easily regulated by tetracycline. In addition, the maximal gene expression level of the tetracycline-controlled gene expression system was higher than that of the wild type CMV promoter system. Therefore, tetracycline-regulated adenoviral vector system could be applicable for regulatory high-level expression of toxic gene. Also, this system will be useful for functional studies and gene therapy.

• IMMUNE NETWORK
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• v.9 no.6
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• pp.265-273
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• 2009

Foot-and-mouth disease virus (FMDV) is a small single-stranded RNA virus which belongs to the family Picornaviridae, genus Apthovirus. It is a principal cause of FMD which is highly contagious in livestock. In a wild type virus infection, infected animals usually elicit antibodies against structural and non-structural protein of FMDV. A structural protein, VP1, is involved in neutralization of virus particle, and has both B and T cell epitopes. A RNA-dependent RNA polymerase, 3D, is highly conserved among other serotypes and strongly immunogenic, therefore, we selected VP1 and 3D as vaccine targets. VP1 and 3D genes were codon-optimized to enhance protein expression level and cloned into mammalian expression vector. To produce recombinant protein, VP1 and 3D genes were also cloned into pET vector. The VP1 and 3D DNA or proteins were co-immunized into 5 weeks old BALB/C mice. Antigen-specific serum antibody (Ab) responses were detected by Ab ELISA. Cellular immune response against VP1 and 3D was confirmed by ELISpot assay. The results showed that all DNA- and protein-immunized groups induced cellular immune responses, suggesting that both DNA and recombinant protein vaccine administration efficiently induced Ag-specific humoral and cellular immune responses.

• Animal cells and systems
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• v.13 no.3
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• pp.275-281
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• 2009

The effects of pentylenetetrazol (PTZ), a GABA receptor antagonist, were studied on passive avoidance learning and expression of heat shock protein 70 (hsp70), neuroglobin, and fatty acid binding protein-7 (fabp-7) genes. Zebrafish were trained to stay in a dark compartment to avoid a weight dropping in an acryl shuttle box with a central sliding door. In two training sessions of 2 h interval, each consisting of 3 trials, the crossing time was significantly increased from $43.2{\pm}14.4s$ to $149.3{\pm}38.5s$ in the first training session and remained $116.1{\pm}36.0s$ s in the first trial of the second training session in the control. In zebrafish treated with PTZ before the first training session, the crossing time was significantly increased neither in the first nor in the second training session. However, the increased crossing time was maintained in the second training session when 10 mM PTZ was treated three times for 10 min at 30 min intervals between the first and second training session. Quantitative real-time PCR showed that expression level of hsp70 mRNA increased two to eight fold over that of control in the brain at 0-24 h after termination of PTZ treatment. No change in expression of neuroglobin and fabp-7 mRNA was shown in PTZ-treated zebrafish. Our studies suggest that PTZ impairs learning ability in avoidance response and also modifies expression of genes related to the neuroprotection.

• BMB Reports
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• v.33 no.5
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• pp.396-401
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• 2000

A differential display method is used to identify novel genes whose expression is affected by treatment with ferric ammonium citrate (FAC) or desferrioxamine (DFO), an iron chelating agent in the human hepatoblastoma cell line (HepG2). These chemicals are known to deplete or increase the intracellular concentration of iron, respectively. Initially, we isolated seventeen genes whose expressions are down- or up regulated by the treatment of the chemicals, as well as their four differentially expressed genes that are designated as clone-1, -2, -3, and -4. These are further characterized by cDNA sequencing and Northern blot analysis. Through the cDNA sequencing, as well as comparing them to genes published using the NCBI BLAST program, we identified the sequence of the clone-1 that is up-regulated by the treatment of DFO. It is identical to the human insulin-like growth factor binding protein-1 (IGFBP-1). This suggests that the IGFBP-1 gene in the HepG2 cell is up-regulated by an iron depletion condition. Also, the expression of the clone-3 and -4 is up-regulated by FAC treatment and their eDNA sequences are identical to the human ferritin-fight chain and human NADH-dehydrogenase, respectively. However, the sequence of the clone-2 has no significant homology to any other known gene. Therefore, we suggest that changes of the cellular iron level in the HepG2 cell affects the transcription of cellular genes. This includes human IGFBP-1, ferritin-fight chain, and NADH-dehydrogenase. Regulation of these gene expressions may have an important role in cellular functions that are related to cellular iron metabolism.

• KSII Transactions on Internet and Information Systems (TIIS)
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• v.10 no.9
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• pp.4145-4164
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• 2016

Energy harvesting is an increasingly attractive source of power for cellular networks, and can be a promising solution for green networks. In this paper, we consider a cellular network with power beacons powering multiple mobile terminals with microwave power transfer in energy beamforming. In this network, the power beacons are powered by grid and renewable energy jointly. We adopt a dual-level control architecture, in which controllers collect information for a core controller, and the core controller has a real-time global view of the network. By implementing the water filling optimized power allocation strategy, the core controller optimizes the energy allocation among mobile terminals within the same cluster. In the proposed green energy cooperation paradigm, power beacons dynamically share their renewable energy by locally injecting/drawing renewable energy into/from other power beacons via the core controller. Then, we propose a new water filling optimized green energy cooperation management strategy, which jointly exploits water filling optimized power allocation strategy and green energy cooperation in cellular networks. Finally, we validate our works by simulations and show that the proposed water filling optimized green energy cooperation management strategy can achieve about 10% gains of MT's average rate and about 20% reduction of on-grid energy consumption.

• Molecules and Cells
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• v.25 no.4
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• pp.479-486
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• 2008

Mitogen-activated protein kinases (MAPKs) play an indispensable role in activation of the myogenic program, which is responsive to mechanical stimulation. Although there is accumulating evidence of mechanical force-mediated cellular responses, the role of MAPK in regulating the myogenic process in myoblasts exposed to cyclic stretch is unclear. Cyclic stretch induced the proliferation of C2C12 myoblasts and inhibited their differentiation into myotubes. In particular, it induced persistent phosphorylation of p38 kinase, and decreased the level of phosphorylation of extracellular-signal regulated kinase (ERK). Partial inhibition of p38 phosphorylation increased cellular levels of MyoD and p-ERK in stretched C2C12 cells, along with increased myotube formation. Treatment with $10{\mu}M$ PD98059 prevented myogenin expression in response to a low dose of SB203580 ($3{\mu}M$) in the stretched cells, suggesting that adequate ERK activation is also needed to allow the cells to differentiate into myotubes. These results suggest that cyclic stretch inhibits the myogenic differentiation of C2C12 cells by activating p38-mediated signaling and inhibiting ERK phosphorylation. We conclude that p38 kinase, not ERK, is the upstream signal transducer regulating cellular responses to mechanical stretch in skeletal muscle cells.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.18 no.9
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• pp.1070-1076
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• 2007

In this paper, we study the characteristics of dual band microstrip antenna with the wedge-shaped radiation patch added the slot using air substrate. Wedge-shaped patch antenna with air substrate is avoided the large probe reactance associated with a thick substrate owing to the use of short probe and good impedance matching over a wide frequency range can be obtained. Slot on the antenna radiator varies the reactance component according to the employed frequency and affect the resonance freaqency of the antenna, therefore the antenna can resonate at the dual band(cellular and PCS band). The slot length and position have an effect on the bandwidth and input impedance of the antenna are optimized by simulation. From the experiment results for the fabricated antenna, -10 dB of $S_{11}$ is content with the allocated bandwidth of Cellular and PCS system and - 15 dB of cross polarization level. From the results of this paper, it has been confirmed that the proposed antenna can be used as the base station antenna fur Cellular and PCS band.

• The Journal of Korean Institute of Electromagnetic Engineering and Science
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• v.15 no.10 s.89
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• pp.1011-1017
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• 2004

Ultra Wideband(UWB) technologies have been developed to exploit a new spectrum resource in substances and to realize ultra-high-speed communication, high precision geolocation, and other applications. The energy of UWB signal is extremely spread from near DC to a few GHz. This means that the interference between conventional narrowband systems and UWB systems is inevitable. However, the interference effects had not previously been studied from UWB wireless systems to conventional mobile wireless systems sharing the frequency bands such as Korean Cellular CDMA and WCDMA. This paper experimentally evaluates the interference from two kinds of UWB sources, namely a direct-sequence spread-spectrum CDMA(DS-CDMA) UWB source and an impulse radio UWB source, to a Cellular CDMA and WCDMA digital transmission system. The average frame error rate degradation of each system are presented. From these experimental results, the interference effects of DS-CDMA UWB source is not severe compared to the Impulse UWB.