• 제목/요약/키워드: cellular function

검색결과 1,363건 처리시간 0.027초

Investigation of Possible Horizontal Gene Transfer from the Leaf Tissue of Transgenic Potato to Soil Bacteria

  • KIM YOUNG TAE;KIM SUNG EUN;PARK KI DUK;KANG TAE HOON;LEE YUN MI;LEE SANG HAN;MOON JAE SUN;KIM SUNG UK
    • Journal of Microbiology and Biotechnology
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    • 제15권5호
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    • pp.1130-1134
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    • 2005
  • To monitor the possibility of horizontal gene transfer between transgenic potato and bacteria in the environment, the gene flow from glufosinate-tolerant potato to bacteria in soils was investigated. The soil samples treated with the leaf tissue of either glufosinate-tolerant or glufosinate-sensitive potato were subjected to PCR and Southern hybridization to determine possible occurrence of glufosinate-resistant soil bacteria and to detect the bar (phosphinothricin acetyltransferase) gene, conferring tolerance to glufosinate. The bar gene was not detected from genomic DNAs extracted at different time intervals from the soil samples, which had been treated with the leaf tissue of either transgenic or non-transgenic potato for 2 to 8 weeks. In addition, the level of glufosinate-resistant bacteria isolated from the soil samples treated with the leaf tissue of transgenic potato was similar to that of the samples treated with non-transgenic potato after 4 months of incubation at $25^{\circ}C$. The bar gene was not detected in the genomic DNAs extracted from colonies growing on the plate containing glufosinate, indicating that the bacteria could acquire the resistant phenotype to glufosinate by another mechanism without the uptake of the bar gene from glufosinate-tolerant potato.

Investigation of Possible Gene Transfer to Soil Microorganisms for Environmental Risk Assessment of Genetically Modified Organisms

  • Kim, Young-Tae;Park, Byoung-Keun;Hwang, Eui-Il;Yim, Nam-Hui;Kim, Na-Rae;Kang, Tae-Hoon;Lee, Sang-Han;Kim, Sung-Uk
    • Journal of Microbiology and Biotechnology
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    • 제14권3호
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    • pp.498-502
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    • 2004
  • The current study was conducted to monitor the possibility of the gene transfer among soil bacteria, including the effect of drift due to rain and surface water, in relation to the release of genetically modified organisms into the environment. Four types of bacteria, each with a distinct antibiotic marker, kanamycin-resistant P. fluorescens, rifampicin-resistant P. putida, chloramphenicol-resistant B. subtilis, and spectinomycin-resistant B. subtilis, were plated using a small-scale soil-core device designed to track drifting microorganisms. After three weeks of culture in the device, no Pseudomonas colonies resistant to both kanamycin and rifampicin were found. Likewise, no Bacillus colonies resistant to both chloramphenicol and spectinomycin were found. The gene transfer from glyphosate-tolerant soybeans to soil bacteria, including Rhizobium spp. as a symbiotic bacteria, was examined by hybridization using the DNA extracted from soil taken from pots, in which glyphosate-tolerant soybeans had been growing for 6 months. The results showed that 35S, T-nos, and EPSPS were observed in the positive control, but not in the DNA extracted from the soilborne microorganisms. In addition, no transgenes, such as the 35S promoter, T-nos, and EPSPS introduced into the GMO soybeans were detected in soilborne bacteria, Rhizobium leguminosarum, thereby strongly rejecting the possibility of gene transfer from the GMO soybeans to the bacterium.

이차원 셀룰라 오토마타에 기반하는 해쉬 함수 (A Hash Function Based on 2D Cellular Automata)

  • 김재겸
    • 한국멀티미디어학회논문지
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    • 제8권5호
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    • pp.670-678
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    • 2005
  • 셀룰라 오토마타(CA, Cellular Automata)란 동역 학계 (Dynamical Systems)를 해석하는 한 방법으로 공간과 시간을 이산적으로 다루고 셀룰라 공간(Cellular Space)의 기본 단위인 각 셀(Cell)이 취할 수 있는 상태를 유한하게 처리하며, 각 셀의 상태가 국소적인 상호작용(Local Interaction)에 의해서 동시에 갱신되는 시스템이다. CA는 그 본질적 인 특성 이 확산(Diffusion)과 국소적 인 상호 작용(Local Interaction)이므로 암호 시스템과 VLSI환경에 적합한 것으로 알려져 있다. 본 연구에서는 새로운 구조의 이차원 셀룰라 오토마타를 제안하고 제안된 이차원 셀룰라 오토마타에 기초한 새로운 구조의 해쉬 함수를 제안하고 제안된 해쉬 함수의 통계적 검증 결과를 밝힌다. 또한 제안된 해쉬 함수는 키를 사용하는 경우와 그렇지 않은 경우에 모두 적용 가능하도록 구성되었다.

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셀룰러 오토마타 변환을 이용한 새로운 워터마킹 (A Novel Watermarking using Cellular Automata Transform)

  • 박영일;김석태
    • 한국정보통신학회:학술대회논문집
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    • 한국해양정보통신학회 2008년도 춘계종합학술대회 A
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    • pp.155-158
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    • 2008
  • 본 논문에서는 2D CAT (Two dimensional Cellular Automata Transform)을 이용한 새로운 블라인드 워터마킹 방법을 제안한다. 먼저 GV (Gateway Values)로 기저함수를 만들고 그것을 이용하여 원 영상을 CA (Cellular Automata) 영역으로 변환한다. 그 후 변환 영역의 특정부분에 랜덤 시퀀스를 워터마크로 삽입한다. 제안방법에서 2D CAT 기저함수는 한 개의 GV에 의해 유일하게 하나가 존재하며 가능한 GV의 종류는 $2^{96}$개가 있기 때문에 뛰어난 보안성 (security)을 가진다. 워터마크 된 영상은 충실도가 기존의 주파수 영역의 방법보다 높을 뿐만 아니라 JPEG 압축, 필터링, 첨예화, 노이즈 등 공격에도 강인성 (robustness)을 가지고 있음을 증명한다.

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세포교정영양요법(OCNT)을 이용한 신체의 항상성 개선 사례 연구 (A Case Study on Improving Body Homeostasis Using Ortho-cellular Nutrition Therapy (OCNT))

  • 홍은아
    • 셀메드
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    • 제14권4호
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    • pp.73.1-73.4
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    • 2024
  • Objective: Case study on improving body homeostasis by ortho-cellular nutrition therapy. Methods: A 48-year-old Korean man underwent OCNT due to symptoms of insomnia and decreased physical function due to extreme chronic stress. Results: After exposure to OCNT, fatigue, sleep quality, and brain fog symptoms improved, and overall physical performance improved, including liver function recovery. Conclusion: For people who suffer from symptoms of decreased physical function in various aspects due to extreme stress, applying OCNT can help alleviate symptoms.

D2D Power Control in the Cellular System: Iterative Algorithm and Convergence

  • Oh, Changyoon
    • 한국컴퓨터정보학회논문지
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    • 제22권9호
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    • pp.41-47
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    • 2017
  • In this paper, we consider the case where D2D users and the cellular user share the uplink of the cellular system. We propose an iterative power control algorithm that converges to the optimum power value. Each user iteratively updates its transmit power level according to the interference function. Finally, all D2D users and cellular user that participate in the transmission get the optimum transmit power level. We first investigate the interference structure and define the interference function. Then, we show that the considered interference function belongs to the standard interference function that converges the unique transmit power level. Through numerical examples, the convergence of the proposed power control algorithm is examined in the various transmission scenarios.

Myosin X and Cytoskeletal Reorganization

  • Ikebe, Mitsuo;Sato, Osamu;Sakai, Tsuyoshi
    • Applied Microscopy
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    • 제48권2호
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    • pp.33-42
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    • 2018
  • Myosin X is one of myosin superfamily members having unique cellular functions on cytoskeletal reorganization. One of the most important cellular functions of myosin X is to facilitate the formation of membrane protrusions. Since membrane protrusions are important factors for diverse cellular motile processes including cell migration, cell invasion, path-finding of the cells, intercellular communications and so on, it has been thought that myosin X plays an important role in various processes that involve cytoskeletal reorganization including cancer progression and development of neuronal diseases. Recent studies have revealed that the unique cellular function of myosin X is closely correlated with its unique structural characteristics and motor properties. Moreover, it is found that the molecular and cellular activities of myosin X are controlled by its specific binding partner. Since recent studies have revealed the presence of various specific binding partners of myosin X, it is anticipated that the structural, biochemical and cell biological understanding of the binding partner dependent regulation of myosin X function can uncover the role of myosin X in diverse cell biological processes and diseases.

Effect of electromagnetic field exposure on the reproductive system

  • Gye, Myung-Chan;Park, Chan-Jin
    • Clinical and Experimental Reproductive Medicine
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    • 제39권1호
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    • pp.1-9
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    • 2012
  • The safety of human exposure to an ever-increasing number and diversity of electromagnetic field (EMF) sources both at work and at home has become a public health issue. To date, many in vivo and in vitro studies have revealed that EMF exposure can alter cellular homeostasis, endocrine function, reproductive function, and fetal development in animal systems. Reproductive parameters reported to be altered by EMF exposure include male germ cell death, the estrous cycle, reproductive endocrine hormones, reproductive organ weights, sperm motility, early embryonic development, and pregnancy success. At the cellular level, an increase in free radicals and $[Ca^{2+}]i$ may mediate the effect of EMFs and lead to cell growth inhibition, protein misfolding, and DNA breaks. The effect of EMF exposure on reproductive function differs according to frequency and wave, strength (energy), and duration of exposure. In the present review, the effects of EMFs on reproductive function are summarized according to the types of EMF, wave type, strength, and duration of exposure at cellular and organism levels.

다 해상도 특성을 갖는 2D 셀룰러 오토마타 변환을 이용한 디지털 워터마킹 (Digital Watermarking using Multi-resolution Characteristic of 2D Cellular Automata Transform)

  • 박영일;김석태
    • 한국통신학회논문지
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    • 제34권1C호
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    • pp.105-112
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    • 2009
  • 본 논문에서는 다 해상도 특성을 갖는 2D 셀룰러 오토마타 변환 (2D Cellular Automata Transform)을 이용한 디지털 워터마킹 방법을 제안한다. 먼저 게이트웨이(gateway) 값들을 선택하여 기저함수를 만든 후 원 영상을 셀룰러 오토마타 영역으로 변환한다. 다음 변환 영역의 특정 부분에 랜덤 비트 옅을 워터마크로 삽입한다. 마지막으로 실험을 통하여 제안 방법의 충실도가 기존의 방법보다 높을 뿐만 아니라 JPEG 압축, 필터링, 첨예화, 노이즈 등 공격에도 강인성을 가지고 있음을 증명한다. 본 제안 방법에서 기저함수는 한 조의 게이트웨이 값들에 의해 유일하게 하나가 존재하며 가능한 게이트웨이 값들의 종류는 $2^{96}$개가 있기 때문에 뛰어난 안전성을 가진다.

MLCA와 CAT를 이용한 새로운 영상 암호화 방법 (A Novel Image Encryption using MLCA and CAT)

  • 박영일;조성진;김석태
    • 한국정보통신학회논문지
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    • 제13권10호
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    • pp.2171-2179
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    • 2009
  • 본 논문에서는 MLCA (Maximum Length Cellular Automata)와 CAT (Cellular Automata Transform)을 이용한 새로운 영상 암호화 방법을 제안한다. 먼저 Wolfram 규칙을 선택하여 규칙행렬을 구성하고 규칙행렬에 의하여 MLCA의 상태 전이행렬 T를 만든 후 암호화 하려는 영상의 픽셀 위치에 따라 전이행렬을 곱하여 픽셀의 값을 변환한다. 다음 게이트웨이 값의 설정에 따라 2D CAT 기저함수를 생성하여 MLCA 암호화한 영상을 CAT 암호화를 한다. 실험결과와 안정성 분석을 통하여 제안한 방법은 높은 암호화 수준과 무손실 암호화의 성질을 가졌음을 확인한다.