• Journal of the Korean Society of Industry Convergence
• /
• v.19 no.3
• /
• pp.133-143
• /
• 2016

The objective in here is to find the density, stiffness, and strength of truss-wall rectangular (TWR) model which is combined with lattice truss (MLT) inside space. The TWR unit-cell model is defined as a unit cell originated from a solid-wall rectangular (SWR) model and it has an empty space inside. Thus, the empty space inside of the TWR is filled with lattice truss model defined as TWR-MLT. The ideal solutions derived of TWR-MLT are based on TWR with MLT model and it has developed by Gibson-Ashby's theory. To validate the ideal solutions of the TWR-MLT, ABAQUS software is applied to predict the density, strength, and stiffness, and then each of them are compared with the Gibson-Ashby's ideal solution as a log-log scale. Applied material property is stainless steel 304 because of cost effectiveness and easy to get around. For the analysis, SWR and TWR-MLT models are 1mm, 2mm, and 3mm truss diameter separately within a fixed 20mm opening width. In conclusion, the relative Young's modulus and relative yield strength of the TWR-MLT unit model is reasonably matched to the ideal expectations of the Gibson-Ashby's theory. In nearby future, TWR-MLT model can be verified by advanced technologies such as 3D printing skills.t.

• Advances in nano research
• /
• v.12 no.5
• /
• pp.501-514
• /
• 2022

This study aimed to investigate the effects and potential mechanisms of Chikusetsusaponin V (CsV) on endothelial nitric oxide synthase (eNOS) and vascular endothelial cell functions. Different concentrations of CsV were added to animal models, bovine aorta endothelial cells (BAECs) and human umbilical vein endothelial cells (HUVECs) cultured in vitro. qPCR, Western blotting (WB), and B ultrasound were performed to explore the effects of CsV on mouse endothelial cell functions, vascular stiffness and cellular eNOS mRNA, protein expression and NO release. Bioinformatics analysis, network pharmacology, molecular docking and protein mass spectrometry analysis were conducted to jointly predict the upstream transcription factors of eNOS. Furthermore, pulldown and ChIP and dual luciferase assays were employed for subsequent verification. At the presence or absence of CsV stimulation, either overexpression or knockdown of purine rich element binding protein A (PURA) was conducted, and PCR assay was employed to detect PURA and eNOS mRNA expressions, Western blot was used to detect PURA and eNOS protein expressions, cell NO release and serum NO levels. Tube formation experiment was conducted to detect the tube forming capability of HUVECs cells. The animal vasodilation function test detected the vasodilation functions. Ultrasonic detection was performed to determine the mouse aortic arch pulse wave velocity to identify aortic stiffness. CsV stimulus on bovine aortic cells revealed that CsV could upregulate eNOS protein levels in vascular endothelial cells in a concentration and time dependent manner. The expression levels of eNOS mRNA and phosphorylation sites Ser1177, Ser633 and Thr495 increased significantly after CsV stimulation. Meanwhile, CsV could also enhance the tube forming capability of HUVECs cells. Following the mice were gavaged using CsV, the eNOS protein level of mouse aortic endothelial cells was upregulated in a concentration- and time-dependent manner, and serum NO release and vasodilation ability were simultaneously elevated whereas arterial stiffness was alleviated. The pulldown, ChIP and dual luciferase assays demonstrated that PURA could bind to the eNOS promoter and facilitate the transcription of eNOS. Under the conditions of presence or absence of CsV stimulation, overexpression or knockdown of PURA indicated that the effect of CsV on vascular endothelial function and eNOS was weakened following PURA gene silence, whereas overexpression of PURA gene could enhance the effect of CsV upregulating eNOS expression. CsV could promote NO release from endothelial cells by upregulating the expression of PURA/eNOS pathway, improve endothelial cell functions, enhance vasodilation capability, and alleviate vessel stiffness. The present study plays a role in offering a theoretical basis for the development and application of CsV in vascular function improvement, and it also provides a more comprehensive understanding of the pharmacodynamics of CsV.

• Proceedings of the Korean Geotechical Society Conference
• /
• 2010.03a
• /
• pp.448-456
• /
• 2010

The mechanical behavior of granular soils is affected by particle bonding including natural cementation. This study addresses a simple model of small strain stiffness and salt concentration based on wave measurements of salt-cemented particulate media. Published models of artificially cemented soils with different curing methods and several types of cementation agents are reviewed. Glass beads with the median diameter of D50 = 0.5mm are prepared in rectangular cells using the water-pluviated method in salt water with different concentrations. Piezo disk elements and bender elements embedded in the cell are used for the measurements of compressional and shear waves. The relationships between elastic wave velocities and salt concentration show an exponential function. The measured small strain stiffness matches well the predicted small strain stiffness based on micromechanics for simple cubic monosized sphere particles. This study demonstrates that the salt concentration in salt-cemented specimen may be evaluated by using elastic wave velocities.

• Proceedings of the Computational Structural Engineering Institute Conference
• /
• 2003.10a
• /
• pp.133-144
• /
• 2003

Generally, diaphragms are installed in the box girder to prevent or decrease the distortion of tile cross section. In engineering practice, diaphragms are spaced in 5m intervals without reasonable basis. ANd the usual diaphragm type is solid-plate type. It is considered to be noneconomical design to the almost design engineers. In this paper, the parametric study was performed to present the design proposal about the diaphragm stiffness and spacing only in tire single cell box girder. For that, the distortional warping normal stress, bending normal stress and transverse bending normal stress were analyzed using finite element program 'SMB' for the accurate structural analysis.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2009.10a
• /
• pp.645-646
• /
• 2009

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.29 no.4 s.235
• /
• pp.563-569
• /
• 2005

This paper develops a finite element model of a cabover type large truck. The finite element model is for evaluating torsional stiffness of the frame of the large truck. The torsional test of the frame is conducted in order to validate the developed finite element model. A load cell is used to measure the load applied to the frame. An angle sensor is used to measure the torsional angle. An actuator is used to apply a load to the frame. A vertical upward load and a vertical downward load are applied to the frame in the torsional test. The frame's torsional stiffness is computed with the measured load and torsional angle in the torsional test. The finite element model of the large truck includes cab, deck and payload, suspension, and tire. Cab, deck, and suspension are modeled not to affect the frame's torsional stiffness. The simulation is performed with the developed finite element model for evaluating the frame's torsional stiffness. The simulation results show a very good correlation with the torsional test results in the tendency of changing of the frame's torsional stiffness not only with the direction of the applying load but also with the amount of the applying load. In addition, the simulation results predict the measured torsional stiffness of the frame with about $5{\%}$ error.

• Journal of the Korean Society of Propulsion Engineers
• /
• v.20 no.5
• /
• pp.31-39
• /
• 2016

This paper is studied on the effect of the bolt joint stiffness on the Thrust Measurement Stand(TMS). TMS is a test stand for thrust performance of the propulsion system, which depends on two factors: The $1^{st}$ is the parallel degree between directions of the thrust vector and action lines of the corresponding measuring load cells for the vector, and the $2^{nd}$ is the orthogonality between action lines of the measuring load cells. Therefore, it is essential to maintain the original shape of the TMS under operating conditions. In this paper, it is examined how the geometric tolerance of the bolt joints and threads of the load cell trains affect the performance of the TMS. Also, some techniques to overcome related problems are proposed.

• BMB Reports
• /
• v.49 no.12
• /
• pp.655-661
• /
• 2016

Polymer brush is a soft material unit tethered covalently on the surface of scaffolds. It can induce functional and structural modification of a substrate's properties. Such surface coating approach has attracted special attentions in the fields of stem cell biology, tissue engineering, and regenerative medicine due to facile fabrication, usability of various polymers, extracellular matrix (ECM)-like structural features, and in vivo stability. Here, we summarized polymer brush-based grafting approaches comparing self-assembled monolayer (SAM)-based coating method, in addition to physico-chemical characterization techniques for surfaces such as wettability, stiffness/elasticity, roughness, and chemical composition that can affect cell adhesion, differentiation, and proliferation. We also reviewed recent advancements in cell biological applications of polymer brushes by focusing on stem cell differentiation and 3D supports/implants for tissue formation. Understanding cell behaviors on polymer brushes in the scale of nanometer length can contribute to systematic understandings of cellular responses at the interface of polymers and scaffolds and their simultaneous effects on cell behaviors for promising platform designs.

• Journal of the Korea Convergence Society
• /
• v.7 no.4
• /
• pp.181-190
• /
• 2016

The objective of this study is to find the effective stiffness and compressive strengths of a unit-cell pinwheel truss and double pinwheel truss model designed following a double helical geometry similar to that of the DNA (deoxyribonucleic acid) structure in biology. The ideal solution for their derived relative density is correlated with a ratio of the truss thickness and length. To validate the relative stiffness or relative strength, ABAQUS software is used for the computational model analysis on five models having a different size of truss diameter from 1mm to 5mm. Applied material properties are stainless steel type 304. The boundary conditions applied were fixed bottom and 5 mm downward displacement. It was assumed that the width, length, and height are all equal. Consequently, it is found that the truss model has a lower effective stiffness and a lower effective yielding strength.

• Proceedings of the KSME Conference
• /
• 2007.05a
• /
• pp.1469-1474
• /
• 2007

In recent years, research to machine large-surface micro-features has become important because of the light guide panel of a large-scale liquid crystal display and the bipolar plate of a high-capacity proton exchange membrane fuel cell. In this study, in order to realize the systematic design technology and performance improvements of an ultra-precision machine for machining the large-surface micro-features, a structural characteristic analysis was performed using its virtual prototype. The prototype consisted of gantry-type frame, hydrostatic feed mechanisms, linear motors, brushless DC servo motor, counterbalance mechanism, and so on. The loop stiffness was estimated from the relative displacement between the tool post and C-axis table, which was caused by a cutting force. Especially, the causes of structural stiffness deterioration were identified through the structural deformation analysis of sub-models.