• Biotechnology and Bioprocess Engineering:BBE
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• 제10권4호
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• pp.304-308
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• 2005

The response of IPTG induction was investigated through the monitoring of the alkali consumption rate and buffer capacity during the cultivation of recombinant E. coli BL21 (DE3) harboring the plasmid pRSET-LacZ under the control of lac promoter. The rate of alkali consumption increased along with cell growth, but declined suddenly after approximately 0.2 h of IPTG induction. The buffer capacity also declined after 0.9 h of IPTG induction. The profile of buffer capacity seems to correlate with the level of acetate production. The IPTG response was monitored only when introduced into the mid-exponential phase of bacterial cell growth. The minimum concentration of IPTG for induction, which was found out to be 0.1 mM, can also be monitored on-line and in-situ. Therefore, the on-line monitoring of alkali consumption rate and buffer capacity can be an indicator of the metabolic shift initiated by IPTG supplement, as well as for the physiological state of cell growth.

• Asian Pacific Journal of Cancer Prevention
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• 제13권12호
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• pp.5947-5954
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• 2012

The cancer stem cell (CSC) model states that cancers are organized in cellular hierarchies, which explains the functional heterogeneity often seen in tumors. Like normal tissue stem cells, CSCs are capable of self-renewal, either by symmetric or asymmetric cell division, and have the exclusive ability to reproduce malignant tumors indefinitely. Current systemic cancer therapies frequently fail to eliminate advanced tumors, which may be due to their inability to effectively target CSC populations. It has been shown that embryonic pathways such as Wnt, Hedgehog, and Notch control self-renewal and cell fate decisions of stem cells and progenitor cells. These are evolutionary conserved pathways, involved in CSC maintenance. Targeting these pathways may be effective in eradicating CSCs and preventing chemotherapy or radiotherapy resistance.

• Molecules and Cells
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• 제25권4호
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• pp.553-558
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• 2008

Essential aspects of the innate immune response to microbial infection appear to be conserved between insects and mammals. In order to identify new Drosophila melanogaster genes involved in the immune response, we performed gene expression profiling of Drosophila SL2 cells stimulated with bacterial (LPS/PGN) or fungal (curdlan) components using a cDNA microarray that contained 5,405 Drosophila cDNAs. We found that some genes were similarly regulated by LPS/PGN and curdlan. However, a large number, belonging to the functional classes of cell organization, development, signal transduction, morphogenesis, cell cycle, and DNA replication, displayed significant differences in their transcription profiles between the two treatments, demonstrating that bacterial and fungal components induce different immune response even in an in vitro cell system.

• Journal of the Korean Society for Industrial and Applied Mathematics
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• 제23권1호
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• pp.39-63
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• 2019

In this paper, we study the effect of Cytotoxic T Lymphocyte (CTL) and antibody immune responses on the virus dynamics with both virus-to-cell and cell-to-cell transmissions. The infection rate is given by Holling type-II. We first show that the model is biologically acceptable by showing that the solutions of the model are nonnegative and bounded. We find the equilibria of the model and investigate their global stability analysis. We derive five threshold parameters which fully determine the existence and stability of the five equilibria of the model. The global stability of all equilibria of the model is proven using Lyapunov method and applying LaSalle's invariance principle. To support our theoretical results we have performed some numerical simulations for the model. The results show the CTL and antibody immune response can control the disease progression.

• IMMUNE NETWORK
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• 제13권5호
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• pp.168-176
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• 2013

In the last few years major progress has been made in better understanding the role of natural killer (NK) cells in hepatitis C virus (HCV) infection. This includes multiple pathways by which HCV impairs or limits NK cells activation. Based on current genetic and functional data, a picture is emerging where only a rapid and strong NK cell response early on during infection which results in strong T cell responses and possible subsequent clearance, whereas chronic HCV infection is associated with dysfunctional or biased NK cells phenotypes. The hallmark of this NK cell dysfunction is persistent activation promoting ongoing hepatitis and hepatocyte damage, while being unable to clear HCV due to impaired IFN-${\gamma}$ responses. Furthermore, some data suggests certain chronically activated subsets that are $NKp46^{high}$ may be particularly active against hepatic stellate cells, a key player in hepatic fibrogenesis. Finally, the role of NK cells during HCV therapy, HCV recurrence after liver transplant and hepatocellular carcinoma are discussed.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2005년도 춘계학술대회
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• pp.353-356
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• 2005

We developed a dynamic model of PEM fuel cell system which can analyze its transient response to dynamic load current. System components such as compressor, air cooler, humidifier, and stack were modeled based on their dynamic equations and performance maps by using Matlab Simulink platform. Through this simulation model, dynamic characteristics of fuel cell system including oxygen excess rat io, stack voltage, and system efficiency were shown. In addition to that, we briefly analyzed the humidity effect on cathode pressure and system efficiency, expecting that this model can be further used to optimize fuel cell system parameters just like operating pressure and temperature, humidity and oxygen excess ratio.

• 한국전기전자재료학회:학술대회논문집
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• 한국전기전자재료학회 2002년도 하계학술대회 논문집 Vol.3 No.2
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• pp.953-956
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• 2002

The electro-optical (EO) performances of a multidomain vertical-alignment (MVA) cell on a homeotropic photopolymer have been investigated. In the absence of an electric field, the MVA cell achieved black state as LC alignment is homeotropic. An electric field created by interdigitated electrodes and rib made after photolithograph using the photopolymer on both substrates causes a director deformation of a multidomain type. Good voltage-transmittance curves for a MVA cell on the homeotropic photopolymer were observed. Also, the stable response time of MVA cell on the homeotropic photopolymer can be achieved.

• 한국전기전자재료학회논문지
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• 제16권3호
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• pp.219-223
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• 2003

The electro-optical (EO) performances of a multidomain verticial-alignment (MYA) cell on a homeotropic photopolymer have been investigated. In the absence of an electric field, the WVA cell achieved black state as LC alignment is homeotropic An electric field created by interdigitated electrodes and rib made after photolithograph using the photopolymer on both substrates causes a director deformation of a multidomain type. Good voltage transmittance curves for a MVA cell on the homeotropic photopolymer were observed. Also, the stable response time of MYA cell on the homeotropic photopolymer can be achieved.

• Journal of the Optical Society of Korea
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• 제19권3호
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• pp.260-264
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• 2015

We propose a negative liquid crystal (n-LC) cell with parallel patterned electrodes for high transmittance and fast response. The proposed cell has higher transmittance and shorter response time than a conventional fringe-field switching cell using n-LCs, because the proposed cell does not have transmittance dips and does have a thinner LC layer.

• 한국미생물·생명공학회지
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• 제50권4호
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• pp.477-487
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• 2022

Probiotics provide a symbiotic relationship and beneficial effects by balancing the human intestinal microbiota. The relationships between microbiota changes and various diseases may predict health abnormalities and diseases. Treatment with vitamins and probiotics is one therapeutic approach. To evaluate the effect of probiotics on vitamin absorption, we chose Lactobacillus rhamnosus CBT-LR5 treatment, which has resistance to vitamin C-inducible toxicity, with vitamins in high-fat diet (HFD)-induced obesity models. CBT-LR5 affected the absorption of micronutrients, such as ionic minerals and water-soluble vitamins. An increase in vitamin C absorption by CBT-LR5 enhanced the antioxidant response in HFD-induced obesity models. Increased vitamin B absorption by CBT-LR5 regulated lipid metabolism in HFD-induced obesity models. These favorable effects of CBT-LR5 on the absorption of vitamins should be investigated as candidate therapeutic target treatments for metabolic diseases.