• Title/Summary/Keyword: cell monitoring

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The Quality of Commercial Salted and Fermented Anchovy Engraulis japonicas Sauces Produced in Korea (국내산 시판 멸치(Engraulis japonicas) 액젓의 품질평가)

  • Um, In-Seon;Seo, Jung-Kil;Kim, Hee-Dai;Park, Kwon-Sam
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.51 no.6
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    • pp.667-672
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    • 2018
  • We investigated the quality of 12 commercial salted and fermented anchovy Engraulis japonicas sauces by measuring their chemical compositions, bacteria concentrations, and biogenic amine contents. The sauces had a moisture content of 67.13-69.83% (mean: 68.17%), salinity of 20.00-25.84% (mean: 22.29%), pH of 5.14-6.28 (mean: 5.63), volatile basic nitrogen content of 119.12-273.37 mg/100 g (mean: 199.19 mg/100 g), total nitrogen content of 0.82-1.50% (mean: 1.30%) and amino nitrogen content of 550.17-1,086.62 mg/100 g (mean: 774.99 mg/100 g). The viable cell counts ranged from not detected to $1.6{\times}10^3CFU/mL$, and the number of biogenic amine-forming bacteria was very low or undetectable. The tested samples contained 372.32-2,111,61 mg/kg (mean 813.48 mg/kg) histamine, 29.62-144.29 mg/kg (mean 98.14 mg/kg) cadaverine, 87.89-530.84 mg/kg (mean 329.91 mg/kg) tryptamine, 20.89-127.17 mg/kg (mean 60.49 mg/kg) putrescine, and 13.08-109.91 mg/kg (mean 57.74 mg/kg) tyramine. Whereas no spermidine or spermine was detected in any sample. These results strongly suggest the necessary of monitoring the biogenic amine contents of commercial salted and fermented anchovy sauces carefully to ensure consumer health.

Design of Simulated Photovoltaic Power Streetlight for Education using Renewable Energy Utilization and Storage Function (신재생에너지 활용 및 저장기능을 이용한 교육용 모의 태양광발전 가로등 설계)

  • Yoon, Yongho
    • The Journal of the Institute of Internet, Broadcasting and Communication
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    • v.21 no.2
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    • pp.137-142
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    • 2021
  • A Photovoltaic power streetlight is a system that uses solar energy to charge a secondary battery and then uses it for night lighting through a lamp, and can be configured as a standalone or grid-connected type by installing an LED streetlight at the load end. The energy generated through the solar cell module can be charged to the secondary battery through the charge/discharge control device, and then the LED street light can be turned on and off by comparing the power generation voltage and the charging voltage according to the monitoring of solar radiation, or by setting a specific time after sunset or sunrise. Based on these contents, this paper designed and manufactured a simulated solar power streetlight for education using new and renewable energy utilization and storage functions. Using these educational equipment, students can 1) understand the flow of energy change using renewable energy including sunlight as electric energy, 2) understand new and renewable energy, and cultivate basic design and manufacturing application power of related products, 3) The use of new and renewable energy through power conversion and strengthening of practical training and analysis through hardware production can be instilled.

Abnormalities of Growth and Morphology in the Attached Diatoms (Ulnaria ulna) according to Heavy Metal Pollution (중금속 오염에 따른 부착규조 (Ulnaria ulna)의 성장 및 형태 변화)

  • Shin, Ra-Young;Ryu, Hui-Seong;Lee, Jung-Ho
    • Journal of Korean Society on Water Environment
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    • v.36 no.6
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    • pp.559-567
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    • 2020
  • The abnomal responseses on growth and morphology of attached diatoms by various heavy metals were studied. Ulnaria ulna (Nitzsch) Compère was employed as experimental species and exposed to the five heavy metals such as Cu, Zn, Pb, Cd, and As with four concentrations (0, 0.01, 0.1, and 2 mg L-1), respectively. The samples of Ulnaria ulna were examined on the changes of cell growth and teratological forms on the 7th, 14th, 21th, and 28th day, respectively, after exposure to the heavy metals. The samples exposed to the highest concentration, 2.0 mg L-1, of all the heavy metals showed the most obvious decreases of growth. The samples exposed to Cd (μ=0.049day-1) and As (μ=0.048day-1) showed the highest decreasing rate of growth (p=0.021(Cd), p=0.002(As)) and the highest morphological changes of diatom valves were also samples exposed to Cd (10.41%) and As (10.13%) (p=0.009 (Cd), p=0.005(As)). In contrast, Pb induced the lowest decreasing rate (μ=0.090 day-1) and the least change in valve morphology (3.31%). The Cd and As showed relatively stronger effects on growth rates compared to Cu, Zn, and Pb. For the percentage of emergence of morphological species by the type, the highest percentage were observed in sampled exposed to type 1 (43.4%) and followed by type 2 (29.1%). The type 2 and 4 were most abundant in samples exposed to Zn and Pb while the type 3 was most abundant in Cd and As. The Cu induced only type 1, suggesting that the frequency of emergence of each type varied among hevay metals. This research suggests that the degrees of abnomal changes on growth rate and valve morphology of Ulnaria ulna can be used as a bioindicater species for heavy metal contamination in freshwater.

Human embryos derived from first polar body nuclear transfer exhibit comparatively abnormal morphokinetics during development

  • Leila Heydari;Mohammad Ali Khalili;Azam Agha Rahimi;Fatemeh Shakeri
    • Clinical and Experimental Reproductive Medicine
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    • v.50 no.3
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    • pp.177-184
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    • 2023
  • Objective: Reconstructed oocytes after polar body genome transfer constitute a potential therapeutic option for patients with a history of embryo fragmentation and advanced maternal age. However, the rescue of genetic material from the first polar body (PB1) through introduction into the donor cytoplasm is not yet ready for clinical application. Methods: Eighty-five oocytes were obtained following in vitro maturation (IVM) and divided into two groups: PB1 nuclear transfer (PB1NT; n=54) and control (n=31). Following enucleation and PB1 genomic transfer, PB1 fusion was assessed. Subsequently, all fused oocytes underwent intracytoplasmic sperm injection (ICSI) and were cultured in an incubator under a time-lapse monitoring system to evaluate fertilization, embryonic morphokinetic parameters, and cleavage patterns. Results: Following enucleation and fusion, 77.14% of oocytes survived, and 92.59% of polar bodies (PBs) fused. However, the normal fertilization rate was lower in the PB1NT group than in the control group (56.41% vs. 92%, p=0.002). No significant differences were observed in embryo kinetics between the groups, but a significant difference was detected in embryo developmental arrest after the four-cell stage, along with abnormal cleavage division in the PB1NT group. This was followed by significant between-group differences in the implantation potential rate and euploidy status. Most embryos in the PB1NT group had at least one abnormal cleavage division (93.3%, p=0.001). Conclusion: Fresh PB1NT oocytes successfully produced normal zygotes following PB fusion and ICSI in IVM oocytes. However, this was accompanied by low efficiency in developing into cleavage embryos, along with an increase in abnormal cleavage patterns.

Resonance frequency analysis of 3D printed self-healing capsules for localization of self-healing capsules inside concrete using millimeter wave length electromagnetic waves (밀리미터 전자기파를 이용한 콘크리트 내부 자가치유 캡슐의 위치 측정을 위한 3D 프린팅 자가치유 캡슐의 공진 주파수 분석)

  • Lim, Tae-Uk;Cheng, Hao;Lee, Yeong Jun;Hu, Jie;Kim, Sangyou;Jung, Wonsuk
    • Proceedings of the Korean Institute of Building Construction Conference
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    • 2022.11a
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    • pp.243-244
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    • 2022
  • In this paper, experiments were conducted on signal amplification of polymer capsules for application to Ground Penetrating Radar so as to enable real-time monitoring of polymer capsules inside concrete using the Morphology Dependent Resonance phenomenon. A TEM CELL and a vector network analyzer were used to analyze the difference in resonance frequency depending on the material of the sphere and the presence or absence of fracture. In order to manufacture a capsule of a size that can be measured using millimeter waves used in GPR, we manufactured a capsule with a 3D printer and analyzed the effects of the presence or absence of coating and the size of the capsule on the resonance frequency. Resonant frequency or signal amplification is more affected by diameter than coating. The capsule showing the highest amplification is the resin-coated 50 mm diameter capsule with a 316-fold increase and the lowest capsule is the uncoated 10 mm diameter capsule with a signal amplification of 11.9 times. These results demonstrate the potential of GPR to measure the position and state of self-healing capsules, which are small-sized polymers, in real time using millimeter waves.

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Development of the Embedded System-based Real-time Internal Status Identification System for Overhead Bin (임베디드 시스템 기반 오버헤드 빈 내부 상황 실시간 식별 시스템 개발)

  • Jaeeun Kim;Hyejung Lim;Sungwook Cho
    • Journal of Aerospace System Engineering
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    • v.17 no.2
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    • pp.111-119
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    • 2023
  • Internal storage state, weight data, and weight-centered calculation values for overhead bin can all be seen in this paper's real-time internal status identification system. The suggested system offers such valuable data via a range of sensors, including load cells and switch arrays. The proposed system could locate internal free space, locate the center of gravity, and give real-time visual information. It was developed utilizing an embedded system and the C programming language. These features led to the creation of smart overhead bins and real-time cargo loading monitoring technologies, both of which could one day aid in the creation of a cargo loading automation system.

A Smart Car Seat System Detecting and Displaying the Fastening States of the Seat Belt and ISOFIX (안전벨트와 아이소픽스의 체결 상태를 감지하여 알려주는 스마트 카시트 시스템)

  • SeungHeun Park;Sangeon Jeon;Beonghoon Kong;seunghwan Kim;Seung Hee Shin;Won-tak Seo;Jae-wan Lee;Min Ah Kim;Chang Soon Kang
    • Journal of Information Technology Services
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    • v.22 no.6
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    • pp.87-102
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    • 2023
  • Existing child car seats do not have a monitoring means for the driver or guardian to effectively recognize the status of whether the seat belt of car seat is fastened and whether the ISOFIX of the car seat is fastened to the inside device of the vehicle. In this paper, we propose a smart car seat system which can monitor in real time, whether the seat belt of a child seated in the car seat is fastened and whether the ISOFIX of the car seat is fastened. The proposed system has been developed with a prototype, in which a Hall sensor, magnet, Bluetooth, and display device are used to detect whether these are fastened and to display the detection results. The prototype system provides the detection results as texts and alarm signal to the display for driver or guardian' smartphone in the car in motion. With functional tests of the prototype system, it was confirmed that the detection functions are properly operated, and the detection results were transmitted to the display device and smartphone via Bluetooth within 0.5 seconds. It is expected that the development system can effectively prevent safety accidents of child car seats.

Metabolomics in Natural Products Research (천연물 연구에서의 메타볼로믹스)

  • Chan Seo;Tae-Su Kim;Bo-Ram Kim;Su Hui Seong;Jin-Ho Kim;Ha-Nul Lee;Sua Im;Jung Eun Kim;Ji Min Jung;Jin-Woo Jeong
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2023.04a
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    • pp.16-16
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    • 2023
  • Metabolomics is the study of global metabolite profiles in a system (cell, tissue, or organism) under a given set of conditions. Metabolomics has its roots in early metabolite profiling studies but is now a rapidly expanding area of scientific research in its own right. In this study, the applications of metabolomics in natural product studies are explored. Ginseng is a well-known herbal medicine and has various pharmacological effects, which include antiaging, anticancer, antifatigue, memory enhancing, immunomodulatory, and stress reducing effects. Metabolomic analysis of organic acids has not been performed for evaluation whether ginseng has been cultivated using conventional or environmental-friendly farming methods. In this study, profiling analysis was conducted for organic acids (OAs) in ginseng roots produced using conventional or environmentfriendly farming methods at five locations in each of five regions. In OA profiles, lactic acid was the most abundant OA in all regions, with the exception for environmentally friendly farmed ginseng in two of the five regions, in which glycolic acid was most abundant OA. OA profiles in all regions showed isocitric acid levels were increased by environment-friendly cultivation, which suggests metabolic differences associated from farming method, and that isocitric acid might be a useful discriminatory biomarker of environmental-friendly and conventional cultivation. The results of the present study suggest metabolomic studies of OAs in ginseng roots might be useful for monitoring whether ginseng has been cultivated using conventional or environmentally friendly farming methods.

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Effects of Surface Treatment of Cathode Materials on the Electrodeposition Behavior of Fe-Ni Alloy (표면처리와 전극 재료가 철-니켈 합금 도금에 미치는 영향)

  • Kang, Na Young;Lee, Jae Ho
    • Journal of the Microelectronics and Packaging Society
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    • v.29 no.4
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    • pp.71-75
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    • 2022
  • In this research, Fe-Ni alloy films were electrodeposited on stainless steel (SS304 and SS430) and Ti plates to investigate the effects of surface conditions of cathode on deposits. The Ti plates were electropolished in 3 M H2SO4-methanol electrolytes at various conditions before electrodeposition, and unpolished Ti and the optimized specimen, polished at 10 V for 8 min, were used as cathode. The anomalous codeposition, the phenomenon which more active Fe is reduced preferentially, occurred on all substrate, however, there were differences in composition of all deposits. As the results of potential monitoring during electrodeposition, it was confirmed that the larger overpotential was applied to the deposition cell when using Ti cathode, leading to high Fe content of deposits from unpolished Ti due to increase in nucleation of Fe. Also, it was founded that the polished Ti can reduced deposition overpotential.

Treatment Response Evaluation of Cardiac Amyloidosis Using Serial T1- and T2-Mapping Cardiovascular Magnetic Resonance Imaging (T1 지도화 기법 심장 자기공명영상 추적 검사를 이용한 심장 아밀로이드증의 치료 반응 평가)

  • Jinwoo Son;Yoo Jin Hong
    • Journal of the Korean Society of Radiology
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    • v.82 no.2
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    • pp.429-434
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    • 2021
  • Amyloidosis is a multisystemic disease characterized by the accumulation of abnormal proteins in extracellular spaces in various organs, with frequent involvement of the myocardium. We report a case of a patient who had cardiac amyloidosis with a trend of reduction in native T1 and T2 values and extracellular volume fraction on serial cardiac magnetic resonance imaging after chemotherapy and stem cell transplantation. The native T1 value and the extracellular volume fraction are closely associated with tissue amyloid burden in amyloidosis patients. This case demonstrated that cardiac magnetic resonance imaging may be used as a non-invasive and quantitative biomarker in the treatment monitoring of amyloidosis.