• Title/Summary/Keyword: cell monitoring

검색결과 724건 처리시간 0.028초

Nickel(II) Determination by Spectrophotometry Coupled with Preconcentration Technique in Water and Alloy Samples

  • Rekha, Dasari;Kumar, Jengiti. Dilip;Jayaraj, Bellum;Lingappa, Y.;Chiranjeevi, Pattium
    • Bulletin of the Korean Chemical Society
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    • 제28권3호
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    • pp.373-378
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    • 2007
  • A micro organism Agrobacterium tumifacient as an immobilized cell on a solid support was presented as a new biosorbent in a simple and sensitive spectrophotometry determination of Ni(II) in various samples using 4-hydroxy benzaldehyde-4-bromophenyl hydrazone as a color developing agent (λmax 497 nm) at pH 4.0 ± 0.2. Beer's law was obeyed over the range of 0.01-0.1 μg L-1. The molar absorptivity and Sandell's sensitivity were 1.285 × 105 L mol-1cm-1 and 0.007245 μg cm-2 respectively. Under these conditions, the preconcentration factor obtained was 82, and the detection limit achieved was 0.05 μg L-1. The detailed study of various interfering ions made the method more sensitive and selective. The recovery of Ni(II) from various samples range from 97.75 to 99.35%. The present method was successfully applied for the determination of Ni(II) in spiked, natural water and alloy samples. The proposed method was compared with reported methods in terms of Student's ‘t'-test and Variance ratio ‘f'-test which indicates that there is no significant difference between proposed and literature method at 95% confidence level.

무창 기공형 태양열 공기 난방 모듈 성능 모니터링 연구 (Performance Monitoring Study of Unglazed Transpired Solar Air Heating Module)

  • 강은철;현명택;이의준
    • 한국태양에너지학회 논문집
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    • 제22권1호
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    • pp.67-72
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    • 2002
  • Solar Air Systems (SAS) have unique advantages for space heating and tempering ventilation air. Air, unlike water, needs no protection against freezing nor are leaks damaging to the building structure or its contents. In contrast to glazed collector, unglazed steel solar collector may have higher efficiencies over glazed flat collector due to the absence of the glass. Therefore, the monitoring of SAS is so important to evaluate actual performance of SAS for right applications. This study is to provide a testing method with a movable test cell developed in KIER to evaluate the thermal performance of SAS based on international standard method ASHRAE 93-86, "Method of Testing to Determine the Thermal Performance of Solar Collectors". The monitoring tool used advanced technique LabVIEW 6i with portable notebook computer. Sample results have been obtained to access the performance of a reference and a target SAS module. The process and tool introduced here could be used to provide a performance verification data for future implementation study applications.

휴대폰 동영상 자기관찰 및 피드백을 통한 기본간호학 실기 교육평가방법에 관한 만족도 - 정체도뇨관 삽입을 중심으로 - (Satisfaction with Evaluation Method for Fundamental Nursing Practical Skill Education Through Cell Phone Animation Self-Monitoring and Feedback - Focus on Foley Catheterization -)

  • 김영희
    • 기본간호학회지
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    • 제15권2호
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    • pp.134-142
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    • 2008
  • Purpose: This study was done to identify satisfaction with the method of evaluating practical skills in fundamental nursing through cellular phone animation self-monitoring and feedback. Method: The participants were 163 first-year student nurses. Data were collected from October 1 to 5 2007 using a structured questionnaire. Results: The average score for satisfaction was 3.65(${\pm}.69$) and the highest score was for the item, "I was pleased to have the opportunity of relearning by looking back at my behaviors through self monitoring" with 4.05(${\pm}.89$). The average score for dissatisfaction was 3.23(${\pm}.90$) and the highest score was "I memorized only orders according to the checklist" with 3.65(${\pm}.97$). The practical tests method showed a significant difference according to motivation for application to college of nursing (F=3.11, p=.047). There was a significant positive correlation between practical education satisfaction and test method (r=.363, p=.000). Conclusion: These findings show that there is a need to develop strategies to improve practical test methods for student nurses.

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Immobilization of Photobacterium Phosphoreum for Monitoring of Toxic Substances

  • Uck-Han Chun;Jun
    • Biotechnology and Bioprocess Engineering:BBE
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    • 제2권2호
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    • pp.141-146
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    • 1997
  • A new sensing system based on the immobilization of luminescent batcteria, Photobacterium phosphoreum, was proposed for continuous real-time monitoring of polluants. The response curves demonstrate that Photobacterium phosphoreum immobilized on the strontium alginate was very sensitive to seven reference chemicals used. The significant inhibitory concentrations for bioluminescence emission were 5 ppm for Pb(NO3)2, NiCl2, CdCl2, 50 ppm for NaAsO2, 0.1ppm for HgCl2, 0.5ppm for pentachlorophenol and less than 5ppm for SDS, respectively. The alginate mixed-cells (AMC) retained their luminescence during experimental period (29 days) under storage condition of -8$0^{\circ}C$. The variables affecting performance of continuous flow through monitoring (CFTM) were optimized in order to ensure stability and efficiency. The flow through cell with strontium-alginate immobilized luminescent bacteria was tested with salicylate and 4-nitrophenol and a rapid response of luminescence was recorded by time drive mode in bioluminescence spectrometer after exposure to both toxicants.

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기포계수식 발효가스 발생량 계측시스템의 개발 (Development of Gas Production Measurement System by Bubble Counting during Fermentation)

  • 이영진;전재근
    • 한국식품과학회지
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    • 제26권3호
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    • pp.195-198
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    • 1994
  • Yeast 발효공정에서 발생되는 가스량을 전자적으로 monitoring 할 수 있는 새로운 기포수 계측 쎈서를 설계, 제작하였다. 본 기포쎈서는 기체를 발생하는 발효공정에 적용할 수 있으며 공정의 상태와 환경에 아무런 변화를 주지 않도록 설계하였다. 그 구조는 가스포집부, 기포형성부, 전자식 기포감지부와 컴퓨터 접속장치로 구성하였다. 기포 계측부는 photo-interrupter카 counter IC 등을 사용하였다. 제작된 쎈서와 컴퓨터 시스템은 효모 배양공정에 활용하여 발효진행과정을 자동적으로 monitoring 하는데 성공하였으며 발효공정에서 발생된 기포수의 값으로 작성된 가스발생량 곡선은 효모의 생육곡선의 양상과 매우 유사한 모양을 보였으며 배양액의 기질영양원이 달리한 발효공정을 monitering한 결과 기질이용 특성도 정확하게 표현할 수 있었다.

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HIFU: 현황 및 기술적 동향 (High Intensity Focused Ultrasound for Cancer Treatment: Current Agenda and the Latest Technology Trends)

  • 서종범
    • The Journal of the Acoustical Society of Korea
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    • 제29권2E호
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    • pp.55-63
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    • 2010
  • High Intensity Focused Ultrasound (HIFU) is a noninvasive surgical method mainly targeting deeply located cancer tissue. Ultrasound is generated from an extemally located transducer and the beam is focused at the target volume, so that selective damage can be achieved without harm to overlying or surrounding tissues. The mechanism for cell killing can be combination of thermal and cavitational damage. Although cavitation can be an effective means of tissue destruction, the possibility of massive hemorrhage and the unpredictable nature of cavitational events prevent clinical application of cavitation. Hence, thermal damage has been a main focus related to HIFU research. 2D phased array transducer systems allow electronic scanning of focus, multi-foci, and anti-focus with multi-foci, so that HIFU becomes more applicable in clinical use. Currently, lack of noninvasive monitoring means of HIFU is the main factor to limit clinical applications, but development in MRI and Ultrasound Imaging techniques may be able to provide solutions to overcome this problem. With the development of advanced focusing algorithm and monitoring means, complete noninvasive surgery is expected to be implemented in the near future.

자동 IV 주사 유량 자동 제어 및 네트워크 모니터링 (Automatic Flow Control and Network Monitoring of IV Injection)

  • 김진남;권원태;이강희
    • 한국생산제조학회지
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    • 제21권1호
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    • pp.161-166
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    • 2012
  • Intravenous (IV) injection is widely used to supply Ringer solution directly into a vein in hospital. Generally, a passive injection method has been used, which causes the inconsistent flow rate of fluid and inappropriate control of injection time by a patient. It leads to an unnecessary nurse's overwork and decrement of IV injection's effect. To solve these problems, flow control infusion pumps have been developed. But because of relatively heavy weight and high price, its usage has been limited. In the present study, a new automatic IV injection system is developed. It is installed with a small pressing mechanism driven by a small electric motor to regulate the flow rate by pressing tube. Proportional integral derivative (PID) feedback control algorithm is applied to control the electric motor. The system is smaller in size and uses lower power than the existing commercial product. The newly developed system is also installed with networking capability, which enables monitoring the status of several automatic IV injection system at the same time.

주목 세포 현탁배양 중, FCW/DCW ratio, 단백질 생산 및 peroxidase활성 조사 (Monitoring of FCW/DCW ratio, Production of Protein and Peroxidase Activity During Suspension Culture of Taxus chinesis)

  • 최형균;윤정환;김상익;송재영;김진현;최호준;홍승서
    • KSBB Journal
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    • 제15권5호
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    • pp.525-528
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    • 2000
  • Taxus chinensis 세포배양 중의 FCW/DCW ratio, 세포내, 외 단백질 생산 및 peroxidase 활성의 변화를 배양시기별로 조사하였다. 조사 결과, 배지내 잔류당이 고갈되기 직전인 배양 14일째에 FCW/DCW ratio 는 12로 가장 낮은 수치를 나타내었고 DCW를 기준으로 한 세포 외에서의 protein 생산도 14일째 가장 낮은 수치인 4.3 mgfg DCW 였다. 배양시기별 P POD 활성도는 단백질 생산 pattern과 유사하였다. 이 결과들 은 flask에서 활발하게 증식 중인 세포들을 이용하여 측정한 것으로 대량배양시, 공정제어를 위한 자료 및 세포가 받는 stress 정도를 나타내는 지표로 활용할 수 있다

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Chemiluminescence System with Air Pump as a Sensor for Determination of Metal Levels in Rain

  • Hong, Hyuck-Gi;Lim, H.B.
    • Bulletin of the Korean Chemical Society
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    • 제26권12호
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    • pp.1937-1940
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    • 2005
  • A lab-made chemiluminescence system with air pump was developed for monitoring of some metal levels in rain. The air pump enabled injection of 17.7 $\mu$g samples into a glass cell filled with luminol-$H_2O_2$ reagent of typically 300 $\mu$L for chemiluminescence measurement. The monitored trend of total metal ions in the rain collected in our campus was compared with analytical results of each metal ion from GFAAS. The system was also demonstrated to determine $Cr^{6+}$ by reduction to $Cr^{3+}$ using $SnCl_2$. The limit of detection for $Cr^{6+}$ obtained by 4 measurements was 85.0 pg $mL^{-1}$ with a relative standard deviation of 3.4%. Although this system doesn’t have selectivity due to the characteristics of chemiluminescence, application of it to environmental monitoring as a sensor for some transition metal ions was demonstrated.

Petri-Net 을 이용한 FMS 제어 및 모니터링 (FMS Control and Monitoring using Petri Net)

  • 김고중;정무영;조현보
    • 대한산업공학회지
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    • 제21권1호
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    • pp.137-151
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    • 1995
  • A difficult problem in operating Flexible Manufacturing Systems (FMS) is to control the system in real-time by coordinating heterogeneous machines and integrating distributed information. The objective of the paper is to present the models and methodologies useful to resolve the difficult problem. The detailed objectives can be described in three folds. First, a hierarchical Colored and Timed Petri-Net (CTPN) is designed to control an FMS in real-time. The concerned FMS consists of a loading station, several machining cells, a material handling system, and an unloading station. Timed-transitions are used to represent the timed-events such as AGV movements between stations and cells, part machining activities in the cells. Signal places are also used to represent communication status between the host and the cell controllers. To resolve the event conflicts and scheduling problems, dispatching rules are introduced and applied. Second, an implementation methodology used to monitor and diagnose the errors occurring on the machines during system operation is proposed. Third, a Petri-Net simulator is developed to experiment with the designed control logic.

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