• Maxillofacial Plastic and Reconstructive Surgery
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• 제33권1호
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• pp.49-54
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• 2011

A case of Behcet's syndrome found in a 22 years old male was is reported along with the histological and immunohistochemical studiesfindings. He The patient had had received prolonged medical therapy of anti-inflammatory agents for the conjunctival ulcer, genital ulcer, oral ulcer, and intestinal ulcers since the first onset of the Behcet's syndrome about approximately 7 years agoearlier. Recently, he felt the athickening of both corners of his lip causing that caused mouth opening difficultydifficulties. A plastic rReconstructive surgery was performed to enlarge the size of the oral orifice by multiple Z-plasty incisions, and finallywhich resulted in proper enlargement of the circumferential length of the lip. During the operation, a scar-like thick fibrous tissue was obtained and examined pathologically. In theThe microscopic observation the revealed the submucosal lesion was to be diffusely fibrosed with the a distribution of sclerotic collagen bundles. Particularly, sSeveral foci of collagen degeneration were found observed in the deep connective tissue, and the degenerating collagen bundles were gradually lost their fibrillar appearance. In the immunohistochemical observations, the foci of collagen degeneration was were strongly positive for IgK, but almost negative for TNF${\alpha}$ lysozyme, and MMP-3. Taken together, it was presumed that tThe submucosal fibrosis was presumed to have firstly presented in this study was probablybeeninduced by the prolonged anti-inflammatory therapy, which may inhibit the removal of sclerosed collagen bundles by the cell-mediated immunity and proteolytic digestion of macrophages, and that it was secondarily aggravated by the deposition of immunoglobulins derived from an autoimmune origin. Therefore, even after the successful plastic surgery of the lip to ameliorate the mouth opening difficulty, the recurrent submucosal fibrosis of lip should be carefully managed in the follow-up treatment.

• Journal of Nutrition and Health
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• 제24권2호
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• pp.97-103
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• 1991

에탄올이 면역계의 항원전달세포이며, 동시에 감마인터페론 등의 림포카인에 의해 활성화되면 작동세포의 역활을 하는 단핵구-대식세포의 arachildonic acid 대사의 cyclooxygenasc 경로의 프로스타글라딘 생산에 미치는 영향을 연구하기 위하여 감마 인터페론으로 전처치하여 활성화시킨 단핵구와 대조군 단핵구를 사용하여 방사면역측정법으로 프로스타글라딘들을 측정하였다. 건강한 성인의 말초혈액으로 Ficoll-Hypaque용액을 이용한 농도구배법과 젤라틴 피복 플라스크를 이용하여 단핵구를 순수하게 분리한 다음 800단위의 감마 인터페론을 사용하여 3일동안 단핵구를 활성화시켰다. 감마인터페론으로 활성화 시키지 않은 $10^6$개의 단핵구에 100mM,300mM,600mM의 에탄올을 30분간 반응시킨 후 6시간후에 thromboxane B$_2$, PGEB$_2$ 와 6-keto-PGF1$\alpha$ 와 PGE$_2$ 모두 대조군에 비하여 생산이 증가하였다. 감마인터페론으로 전처치한 경우에는 알코올 처치에 관계없이 세가지 프로스타글란딘들 모두 6시간과 24시간에서 대조군보다 감소되었다. 이와같은 성적들로 미루어 볼 때 감마인터페론 처치가 에탄올로 유발한 단핵구 arachidonic acid 대사의 변화를 억제한다고 사료된다.

• Journal of Chest Surgery
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• 제30권3호
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• pp.330-335
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• 1997

아스페르길루스 감염증은 드물기는 하지만 면역 억제된 상황에서는 비교적 잘 발생하며 치사율도 높은 것으로 알려져 있다. 경구 또는 정맥내 치료제가 있기는 하지만 합병증이 많고 장기간 사용해야 하기 때문에 치료효과는 크게 기대할 수 없는 경우가 많다. 따라서 환자에 따라서는 외과적 요법을 병행하여 치료효과를 높이는 방법도 사용하고 있다. 세종병원에서는 지금까지 실시한 15례의 심장이식환자중 2 례의 폐 아스페르길루스증을 경 험하였기 에 보고하는 바이다. 두 환자 모두 나이와 수술시기가 비슷하였 으며 수술후 약 3주후 외래에서 흉부 X-선상 폐병변을 발견하였다 경피적 천자생검상 Aspergillus fumigatus로 확인되어 정맥내 Amphotericin B와 경구용 Itraconazole을 병용한 항진균제요법을 실시하 였으며 두번째 환자는 병변이 우하엽에 국한되어 외과적 절제술을 병용하였다. 첫번째 환자는 이식후 14개월째 양호한 상태로 추적중이며 두번째 환자는 치료 완료후 3개월째 비세포성 거부반응으로 사망하였다.

• Nutritional Sciences
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• 제1권1호
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• pp.22-28
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• 1998

Plasma concentrations of Vitamins E and A were measured in 15 non-insulin dependent Korean female subjects and 15 age-matched normal subjects using reversed-phase high-performance liquid chromatography. No differences were found in plasma Vitamin E concentrations between the 2 groups. Plasma Vitamin A concentrations were higher in subjects with non-insulin dependent diabetes melitus (NIDDM). The effects were evaluated of 4 weeks of daily supplementation of 400 mg Vitamin E on plasma levels of these two vitamins. In addition, the effects were observed for Vitamin E supplementation on oxidative stress and immune-related compound productions in non-insulin dependent diabetic patients and control subjects. After treatment with Vitamin E, plasma Vitamin E concentrations were significantly elevated in both groups. Basal plasma thiobarbituric acid reactive substances (TBABS) were identical, and a decreased level of TBARS caused by Vitamin E was observed only in the diabetic group (0.02739$\pm$0.0024 versus 0.01814$\pm$0.0008 nmols malondialdehyde equivalents/dl plasma ; p<0.05). The basal and after-treatment levels of immunoglobulins A, G, M were identical in control and diabetic groups, indicating that Vitamin E did not appear to alter gross humoral responses in this study. However, elevation of Complement 3 ($C_3$) was noticed due to Vitamin E supplementation, revealing a possible effect of vitamin E on one aspect of humoral immunity, Furthermore, an increase in prostaglandin E_2 ($PGE_2$) levels in diabetic patients was normalized by Vitamin E supplementation. This suggests indirectly that the depressed cell-mediated response due to elevated $PGE_2$ could be normalized. For the definitive antioxidant intake recommendations for prevention and treatment of adverse effects of non-insulin dependent diabetes, evidence from intervention trials like this study should be collected. The present data suggests that Vitamin E may oxen some protective effects against oxidative damage and might have beneficial effects of partial immune-stimulation.

• IMMUNE NETWORK
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• 제3권3호
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• pp.182-187
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• 2003

Background: The mushroom Phellinus linteus (PL) has been shown to have the anti-tumor and immunostimulatory effects. We hypothesized that the hot water extract of PL (WEPL) exerts its significant immunostimulatory effect by inducing production of the Th1-derived cytokine interferon-${\gamma}$ (IFN-${\gamma}$) by T lymphocytes. Methods: T lymphocytes were isolated from the mice fed with 200 mg/kg of WEPL once a day for 4 weeks, and then stimulated with the mitogen concanavaline A (Con A). IFN-${\gamma}$ gene and intracellular protein expressions were analyzed by RT-PCR and flow cytometry, respectively. The production of IFN-${\gamma}$ was measured by enzyme-linked immunosorbent assay. Results: WEPL significantly enhanced the transcription of IFN-${\gamma}$ mRNA. The effect of WEPL on IFN-${\gamma}$ expression was further supported by a concomitant increase in the number of cells with intracellular IFN-${\gamma}$ protein as well as the secretion of IFN-${\gamma}$. However, WEPL did not modulate either gene expression or protein secretion of interleukin-4, a Th2-associated cytokine, by Con A-stimulated T lymphocytes. Conclusion: Our results demonstrate that one of the potentially beneficial anti-tumor and immunostimulatory effects of WEPL may be mediated through the enhancement of IFN-${\gamma}$ secretion by T lymphocytes.

• Tuberculosis and Respiratory Diseases
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• 제47권5호
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• pp.697-703
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• 1999

Mycobacterium celatum is a recently described nontuberculous mycobacterium. Even though pulmonary or lymphatic infection cases were reported previously in human, the clinical significance of the infection with M. celatum is not yet understood completely. Mast infections with this species occurred in the patients with suppressed cell-mediated immunity such as AIDS, and there are only a few cases of pulmonary infection with M. celatum in immunocompetent adults or infants in the world. In Korea, mycobacterial pulmonary infection is a major problem of respiratory disease but, there has been no pulmonary infection with M. celatum reported. We report, to our knowledge, the first Korean case of pulmonary infection with M. celatum, which was identified by rpoB genomic sequencing.

• Journal of Yeungnam Medical Science
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• 제10권2호
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• pp.518-524
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• 1993

저자 등은 만성질환으로 인해 영양결핍 및 면역저하상태로 생각되는 75세 폐결핵환자에서 분선충의 대량의 자가감염을 객담, 분변에서 확인하여 치료하였으나 사망한 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권8호
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• pp.1164-1169
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• 2001

A five-week trial was conducted to evaluate the effect of organic chromium from different sources on growth performance, immune response and serum parameters of weaned pigs. One hundred and eighty Tianjin white pigs weaned at $35{\pm}1$ days of age, were allotted to three treatments with six replicates and10 pigs per pen. Pigs were fed corn-soybean-whey-fishmeal basal diets with either no supplemental Cr, $200{\mu}g/kg$ Cr as chromium picolinate (CrPi), or $200{\mu}g/kg$ Cr as chromium yeast (Cr-yeast). To assess humoral immune response, all pigs were immunized with swine fever virus on day 21 and two pigs from each pen were immunized with pure albumin on day 14. Cell-mediated immunity was measured by determining the double skinfold thickness (DST) of two pigs from each pen before and 24h after stimulation with phytohemagglutinin (PHA) on day 28. The results indicated that: (1) diets with Cr-yeast increased average daily gain (ADG, p<0.05) and tended to increase average daily feed intake (ADFI, p<0.10). Diets with CrPi did not increase ADG and ADFI (p>0.05). (2) Dietary CrPi or Cr-yeast supplementation did not affect blood urea nitrogen, glucose, or cholesterol (p>0.05), but blood urea nitrogen in CrPi and Cr-yeast supplemented groups and blood glucose in the Cr-yeast supplemented group were significantly influenced by sampling days (p<0.05). (3) Serum proteins (TP, ALB, and GLB) were influenced by sampling days (p<0.05), but not by dietary Cr treatment (p>0.10). (4) There were no significant differences among treatments in the titers of albumin antibody and swine fever virus antibody (p>0.05) or DST before and after PHA stimulation (p>0.05), indicating that organic chromium has no significant effect on the immune function of weaning pigs. Therefore, these results agree with other research that the effects of supplemental Cr are variable in weanling pigs.

• Asian-Australasian Journal of Animal Sciences
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• 제18권4호
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• pp.524-531
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• 2005

Two experiments were conducted to standardize in ovo injection of amino acids (AA) and to evaluate the effect of in ovo injection of limiting AA(s) on pre and post hatch growth performance, immune response and development of digestive organs. Combinations of essential and non-essential amino acids (Lys+Arg, Lys+Met+Cys, Thr+Gly+Ser, Ile+Leu+Val and Gly+Pro) were injected into 50 eggs in each treatment group at 14 d of embryonic age. Standardization of injection site, needle length and embryonic age revealed that when AA were injected in to the broad end of the egg with a 11 mm needle and at the narrow end with a 24 mm needle both at the 7$^{th}$ and 14$^{th}$ d of incubation there was poor hatchability. However, better hatchability was recorded when the AA were injected in the narrow end of the egg with a 11 mm needle and in the broad end with a 24 mm needle on the 14$^{th}$ d of incubation. The chick to egg weight ratio was higher (p<0.018) when AA were injected on the 14$^{th}$ d of incubation. When a combination of amino acids were injected a 63.6 or 63.2 g difference in body weight of bird at 21 d was recorded between uninjected control and Ile+Leu+Val or Gly+Pro group, respectively. Higher feed intake (p<0.047) was recorded in the AA injected groups and feed conversion ratio (FCR) was numerically better in Gly+Pro, Lys+Met+Cys AA injected groups than in the uninjected control. Significantly higher immune response to cell mediated (p<0.033) and humoral (p<0.002) immunity was observed in in ovo amino acid injected birds, especially in Lys+Met+Cys, Thr+Gly+Ser or Ile+leu+Val groups. The digestive organ weights at 21 d did not differ between specific AA injected groups and the uninjected control. In ovo injected amino acids may act as immunomodulators and their role in gastrointestinal development needs further research.

• 대한미생물학회지
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• 제21권2호
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• pp.251-259
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• 1986

This study was performed to assess the effect of inosiplex(ISP) on the resistance of mice Candida albicans infection, the migration of chicken leukocytes, the production of leukocyte migration inhibitory factor(LIF), and the cell-mediated immunity(CMI) to lepomin in multibacillary lepromatous leprosy patients. The treatment with ISP before or on the time of infection with C. albicans had no or deliterious effect, and treatment with ISP after infection had no effect on the recovery of C. albicans from the kidneys of mice. The migratory ability of chicken leukocytes and the production of LIF from splenocytes of mice were not affected by ISP treatment. However, ISP decreased the migration of chicken leukocytes in vitro, and this decrease was dose-dependent. The therapy of lepromatous leprosy patients with ISP for 10 or 30 days clearly showed the increase of the significant positive rate of Mitsuda skin test to lepromin. The immune recovery as a result of the therapy was found to be the best in the group of patients treated for 30 days. This results suggest that (1) the effect of ISP in renal candidiasis can vary depending on the time of treatment relative to infection, (2) ISP can primarily change the migratory ability of chicken leukocytes but does not affect the production of LIF in mice, and (3) the classical therapy combined with ISP can reinforce or restore the defences of lepromatous leprosy patients against Mycobacterium leprae.