• 대한기계학회논문집B
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• 제20권3호
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• pp.1114-1122
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• 1996

Variation of temperature field in a Hele-Shaw convection cell was measured by using a HSI true color image processing system and TLC(Thermochromic Liquid Crystal) solution. The relationship between the hue value of TLC color image and real temperature was obtained and this calibration result was used to measure the true temperature. The temperature field in the Hele-Shaw convection cell shows periodic characteristics of 45 sec at Ra = 9.3 * 10$\^$6/. The temperature field measurement technique developed in this study was proved to be a useful and powerful tool for analyzing the unsteady thermal fluid flows.

• 한국멀티미디어학회논문지
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• 제20권5호
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• pp.740-747
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• 2017

A fast cell contour extraction method using CUDA parallel processing technique is presented. The cell contour extraction is one of important processes to analyze cell information in pathology. However, conventional sequential contour extraction methods are slow for a huge high-resolution medical image, so they are not adequate to use in the field. We developed a parallel morphology operation algorithm to extract cell contour more quickly. The algorithm can create an inner contour and fail to extract the contour from the concave part of the cell. We solved these problems by subdividing the contour extraction process into four steps: morphology operation, labeling, positioning and contour extraction. Experimental results show that the proposed method is four times faster than the conventional one.

• 한국빅데이터학회지
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• 제4권1호
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• pp.53-61
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• 2019

주변 세포의 구조적, 생화학적 지지체를 제공하는 세포 외 기질은 세포의 분열과 분화 등을 좌우하는 세포생리 조절인자이다. 바이오 분야에서는 3차원 조직공학 지지체인 스캐폴드를 제작하고, 제작한 스캐폴드에 줄기세포를 배양해 동물에 이식해 조직 재생력을 평가한다. 이는 조직 내 콜라겐과 같은 구성성분에 좌우된다. 따라서 조직 내 구성성분의 포함율 및 분포를 파악하는 것이 매우 중요한데, 이에 관한 데이터를 염색된 조직 이미지의 색상을 분석함으로써 얻어낸다. 이때 이미지 수집부터 분석까지의 과정이 적지 않은 비용이 소모되고 있고, 수집되고 분석된 데이터를 연구 기관마다 상이한 포맷으로 관리하고 있다. 따라서 데이터 통합관리 및 분석결과 검색 등이 이루어지지 않고 있다. 본 논문에서는 관련 빅데이터를 통합적으로 관리할 수 있는 데이터베이스를 구축하고, 이 연구 분야에서 중요한 분석 척도인 색상을 기준으로 검색할 수 있는 바이오 이미지 통합 관리 및 검색 시스템을 제안한다.

• Journal of Biosystems Engineering
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• 제38권1호
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• pp.55-63
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• 2013

Purpose: The present study aimed at image processing methods for automatic cell pattern recognition and morphological analysis for tissue engineering applications. The primary aim was to ascertain the novel algorithm of adaptive brightness correction from microscopic images for use as a potential image analysis. Methods: General microscopic image of cells has a minor problem which the central area is brighter than edge-area because of the light source. This may affect serious problems to threshold process for cell-number counting or cell pattern recognition. In order to compensate the problem, we processed to find the central point of brightness and give less weight-value as the distance to centroid. Results: The results presented that microscopic images through the brightness correction were performed clearer than those without brightness compensation. And the classification of mixed cells was performed as well, which is expected to be completed with pattern recognition later. Beside each detection ratio of hBMSCs and HeLa cells was 95% and 92%, respectively. Conclusions: Using this novel algorithm of adaptive brightness correction could control the easier approach to cell pattern recognition and counting cell numbers.

• Journal of Biosystems Engineering
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• 제22권4호
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• pp.433-438
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• 1997

The freshness of apple was evaluated by characterizing the surface texture of flesh cells. The freshness index which was related to the amount of wrinkles on the cell surface was defined to quantify the freshness. Four parameters relevant to the amount of the cell wrinkles were selected and measured using image analysis including wrinkle extraction procedure and Fast Fourier Transform of a wrinkle-extracted image. Out of 4 parameters, three parameters had highly significant correlations with the time elapsed after harvest. But it was concluded that two parameters out of such 3 parameters could be used for description of freshness index.

• 대한의용생체공학회:의공학회지
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• 제10권2호
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• pp.106-108
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• 1989

A new method of digital image analysis technique for discrimination of cancer cell was presented in this paper. The object image was the Thyroid eland cells image that was diagnosed as normal and abnormal (two types of abnormal: follicular neoplastic cell, and papillary neoplastic cell), respectively. By using the proposed region segmentation algorithm, the cells were segmented into nucleus. The 16 feature parameters were used to calculate the features of each nucleus. A9 a consequence of using dominant feature parameters method proposed in this paper, discrimination rate of 91.11% was obtained for Thyroid Gland cells.

• 대한의용생체공학회:의공학회지
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• 제33권4호
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• pp.169-176
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• 2012

Tumor cell morphology is closely related to its migratory behaviors. An active tumor cell has a highly irregular shape, whereas a spherical cell is inactive. Thus, quantitative analysis of cell features is crucial to determine tumor malignancy or to test the efficacy of anticancer treatment. We use 3D time-lapse phase-contrast microscopy to analyze single cell morphology because it enables to observe long-term activity of living cells without photobleaching and phototoxicity, which is common in other fluorescence-labeled microscopy. Despite this advantage, there are image-level drawbacks to phase-contrast microscopy, such as local light effect and contrast interference ring. Therefore, we first corrected for non-uniform illumination artifacts and then we use intensity distribution information to detect cell boundary. In phase contrast microscopy image, cell is normally appeared as dark region surrounded by bright halo ring. Due to halo artifact is minimal around the cell body and has non-symmetric diffusion pattern, we calculate cross sectional plane which intersects center of each cell and orthogonal to first principal axis. Then, we extract dark cell region by analyzing intensity profile curve considering local bright peak as halo area. Finally, we calculated the Fourier descriptor that morphological characteristics of cell to classify tumor cells into active and inactive groups. We validated classification accuracy by comparing our findings with manually obtained results.

• 한국광학회지
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• 제17권6호
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• pp.519-524
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• 2006

일반적으로 Lohmann형 이진 홀로그램에서 홀로그램의 진폭과 위상을 표현할 때 사각 셀이 이용된다. 본 논문에서는 기존의 사각 셀 대신에 원형 셀을 이용하여 진폭과 위상을 표현하는 방법을 시도하였으며, 기존의 사각 셀과 본 논문에서 사용한 원형 셀을 이용하여 구현된 컴퓨터 형성 홀로그램(CCH)의 특성을 비교하기 위하여 컴퓨터 실험과 광학적 실험 데이터를 토대로 재생된 결과를 비교 분석하였다. 실험 결과 원형 셀을 여러 픽셀로 나눌 경우 보다 뚜렷한 재생상을 얻을 수 있었으며, 원형 셀이 사각 셀 보다 균일할 재생상을 얻는 측면에서는 다소 차이가 있었으나 재생시에 원형 셀이 사각 셀 보다 더욱 밝은 재생상을 얻을 수 있었다.

• 한국멀티미디어학회논문지
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• 제17권10호
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• pp.1150-1159
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• 2014

This paper proposes a method for extracting tissue cells from an organism image by an electron microscope and getting the whole cell number and the area from the cell. In general, the difference between the cell color and the background is used to extract tissue cell. However, there may be a problem when overlapped cells are seen as a single cell. To solve the problem, we split them by using cell size and curvature. This method has a 99% accuracy rate. To measure the cell area, we compute two areas, the inside and boundary of the cell. The inside is simply calculated by the number of pixels. The cell boundary is obtained by applying super sampling, linear interpolation, and cubic spline interpolation. It improves the error rate, 18%, 19%, and 120% respectively, in comparison to the counting method that counts a pixel area as 1.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제9권12호
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• pp.5087-5102
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• 2015

Rapid popularization of smart cell phone equipped with camera has led to a number of new legal and criminal problems related to multimedia such as digital image, which makes cell phone source identification an important branch of digital image forensics. This paper proposes a classifier combination based source identification strategy for cell phone images. To identify the outlier cell phone models of the training sets in multi-class classifier, a one-class classifier is orderly used in the framework. Feature vectors including color filter array (CFA) interpolation coefficients estimation and multi-feature fusion is employed to verify the effectiveness of the classifier combination strategy. Experimental results demonstrate that for different feature sets, our method presents high accuracy of source identification both for the cell phone in the training sets and the outliers.