• KSBB Journal
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• v.16 no.6
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• pp.579-591
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• 2001

The cell growth inhibitor effect of cervical cancer cells was investigated by liposome mediated transfection (pRcCMVp53/lipofectin) and by transfection using adenovirus (AdCMVp57). The papilloma virus cancer cell lines we used in this study were HPV16 positive, having inhibiter gene, wild p53 gene, CaSki, SiHa, HPV18 positive HeLa, HeLaS3 and HPV negative C33A, HT3. LacZ gene of E.coli was used as the marker gene for the transfection efficiency. The effect on the inhibition of tumor cell growth was measured by cell count and cell viability though ELISA analysis and MTT assay. The inhibition of tumor cell growth was confirmed by measuring each assay for six days, comparing with the normal control cell growth. The cell growth of cervical cancer calls by transfection was significantly reduced and showed tittle differences among the cell lines. To eliminate the potential problem of Ad(adenovirus) contamination during rAAV production, rAAV can be produced by a triple transfection of vector plasmic, packaging plasmid, and adenovirus helper plasmid. To examine the helper functions of Ad plasmids on the production of rAAV vector, we carried out cotransfection of three plasmids, AAV vector, packaging construct, and Ad helper plasmids. The optimized transfection condition for calcium phosphate method is 25ug of total DNA per 10-cm-diameter plate of 293 cell. We found that rAAV yields peaked at 48hr after Ad infection. The titer of rAAV was measured by the dot blot analysis to measure the number of particles/ml based on the quantification of viral DNA. Recent1y, Kombucha(fungi) was identified as a very potent antileukefic agent. In the present study, effect of natural toxin(plankton) and Kombucha is PSP(GTXI-3, neoSTX), on various MTT assay cervical cancer cell line. Toxin(GTX 1-3, neoSTX) also inhibited the proliferation in primary cervical cancer calls in a dose-dependent toxin concentration. These results showed that toxin was very potent in inhibiting the proliferation of cervical cancer calls in vitro. Toxins and Kombuoha exhibited a dose dependent inhibition of cellular proliferation in cancer cell line.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2004.10a
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• pp.1462-1465
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• 2004

A new method to fabricate metal electrodes on side wall of the microchannel is presented. Coulter counter allows to count the number of cell passing through the microchannel by detecting impedance variation between two electrodes. The relative position of two electrodes is important for sensitivity of impedance measurement. 100nm thick Al electrodes are deposited on the channel side wall by means of shadow evaporation.

• Korean Journal of Pharmacognosy
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• v.20 no.4
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• pp.233-242
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• 1989

The effect of Codonopsis ussuriensis on red blood cell(RBC) count in rats was investigated. Among various fractions tested, a significant increase of RBC was observed in animal group treated with ether extract. From the ether soluble fraction, two compounds were isolated and their structures were elucidated as ${\alpha}-spinasterol$ and ${\beta}-sitosterol$ by chemical and spectral analyses.

• Archives of Pharmacal Research
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• v.14 no.4
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• pp.370-378
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• 1991

Effects of squalene on humoral immune system in mice were investigated. Squalene exhibited significant increases in the circulating leukocyte counts and relative spleen and thymus weights of the mice. However, the relative liver weight was slightly decreased. Hemagglutination titers (HA) were signficantly enhanced by squalene while Arthus reaction was not affected. Splenic plaque forming cells (PFC) were also greatly increased by squalene, especially at doses of 50 and 100 mg/kg of it.

• 대한예방의학회:학술대회논문집
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• 1998.10b
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• pp.88-88
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• 1998

• CELLMED
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• v.13 no.11
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• pp.38.1-38.9
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• 2023

Objective: A case report on the Leukopenia Patients Using Ortho-Cellular Nutrition Therapy (OCNT) Methods: A Korean female in her 50s suffering from the rare side effect of leukopenia after taking athlete's foot medicine (Terbinafine HCl) Results: The results revealed that the white blood cell count, which had dropped before the OCNT, had improved to a normal level. Conclusion: The application of the OCNT can help change health-related figures in patients with relevant problems.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6047-6053
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• 2012

Bracken fern [Pteridium aquilinem (L.) kuhn (Dennstaedtiaceae)] is one of the most common species on the planet. It has been consumed by humans and animals for centuries. Use by some human groups is because they believe bracken fern is good for health as plant medicine. However, it is also one of the few known plants that can cause tumors in farm animals. Many interested groups have focused their attention on bracken fern because of these interesting features. In order to evaluate the biological effects of exposure to this plant in cellular level, human cancer cell lines were treated with the fern dichloromethane extracts and the genotoxic and cytotoxic effects were studied. Anti-proliferative/cytotoxic effects were evaluated by cell count, MTT assay and flow cytometry methods with three different cancer cell lines, TCC, NTERA2, and MCF-7, and two normal cells, HDF1 and HFF3. Pro-apoptotic effects of the extracts were determined by DAPI staining and comet assay, on TCC cancer cells compared to the normal control cell lines. Cellular morphology was examined by light microscopy. Our present study showed that the extract caused DNA damage and apoptosis at high concentrations ($200{\mu}g/mL$) and also it may induce cell cycle arrest (G2/M phase) at mild concentrations (50 and $30{\mu}g/mL$) depending on the cell type and tumor origin. These results indicate that bracken fern extract is a potent source of anticancer compounds that could be utilized pharmaceutically.

• Proceedings of the Ginseng society Conference
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• 2002.10a
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• pp.376-384
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• 2002

Object To find out which of the 27 ginsenosides isolated from Panax ginseng C.A. Mey that may inhibit the proliferation of human osteosaocoma cell line $U_2OS$. Methods Effects of each individual ginsenoside on the proliferation of $U_2OS$ cell were studied by determining the viability of cancer cells during culture with or without the presence of the test compound. DNA assay was determined by flow cytometry. Results Ginsonosides -Ro, $-Rh_l,\;-Rh_2,\;-F_1\;and\;-L_8$ at concentrations of 5 ,umol/L could obviously suppress the proliferation of $U_2OS$ cells while ginsenosides $-Rg_1,\;-F_3,$ -Rf, PPT and PT significantly inhibited the cancer cells. Flow cytometry revealed that ginsenosides $-Ro,-Rg_1-Rf,-F_1-Rh_2,PPT$ and PT induced cell cycle arrest at $G_0/G_1$ phase with obvious decrease of cell count at Sand $G_2+M$ phase, Moreover, ginsenosides $-Rf_1,-Rg_1,\;-F_1$ and PPT induced significantly high rates of cell death as compared with the control. Conclusion These data suggested that ginsenosides inhibited $U_2OS$ proliferation Via cell cycle arrest or induction of cell death.

• Korean Journal of Veterinary Research
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• v.47 no.1
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• pp.85-89
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• 2007

A case of multicentric high grade T cell lymphoma is reported in a 5-year-old male Maltese dog with generalized lymphadenopathy. The dog showed depression, anorexia, blindness, jaundice, arrhythmia, and hematuria for 8 months. Complete blood count and chemistry profile revealed anemia and increased alanine transferase, alkaline phosphatase, total bilirubin, and total cholesterol. Grossly, most of lymph nodes, spleen, and liver were enlarged and neoplastic masses were occupied in these tissues. Histologically, massive accumulation of small noncleaved neoplastic lymphocytes with high mitotic figures was observed in all lymph nodes and spleen. Infiltration of neoplastic lymphocytes was also noted in the lung, liver, kidney, eye, skin, muscle, and bone marrow of femur. Immunohistochemistry revealed that tumor cells were CD3-positive and but CD79a-negative, consistent with T-cell lineage. In our best knowledge, this is the first report of multicentric lymphoma clarified the origin of tumor cells in Korea.

• Restorative Dentistry and Endodontics
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• v.16 no.1
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• pp.158-166
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• 1991

In order to investigate the cytotoxicity of composite resin in vitro, BALB / C mouse fibroblast were cultured in MEM in which silux, P-50, microrest, clearfil, amalgam and glass - ionomer, in shape of $2{\times}9mm$ circular disk. The experiments were- performed by cell count on 4 hours, 1, 3, 6 days and the composite resin groups, amalgam, glass - ionomer were compared. 1. On the sixth day, the cellular number of resin composite groups were remarkedly reduced, in contrast, the that of amalgam and glass - ionomer group continuously increased. 2. It was only on the 4 hours that the cellular number contained in amalgam were reduced, but increased thereafter, and the cellular number contained in glass - ionomer are greater than other groups. 3. In resin group, especially between self - curing resin and light - curing resin, there is no difference in cellular number statistically (p>0.05). 4. It was amalgam where the round cell without cellular process was found on the 4. hours and on the 6 th day the cell without cellular process was found numeroulsy in resin group whereas in amalgam and glass - ionomer, like control group was contained cell forming monolayer. These result suggested that the toxicity of the self - curing and light - curing resin greater than that of the amalgam and glass - ionomer.