• Korean Journal of Veterinary Research
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• v.29 no.2
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• pp.51-57
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• 1989

This study was performed to investigate the toxicity of ochratoxin A (OA) to the chromosomes of $K_{562}$ tumor cell-line in vitro. The results of this experiment were as follows: 1) Chromosomes of $K_{562}$tumor cell-line resulted in pseudotriploidy on the control group. Chromosomes of $K_{562}$ tumor cell-line treated with OA resulted in heteroploidy compared with the control group. The mean number of chromosomes in the karyotype of the control group (60) were 7 in the A group, 5 in the B group, 20 in the C+X group, 7 in the D group, 9 in the E group, 6 in the F group, and 6 in the G+Y group respectively. The number of chromosomes were increased as follows: Treating with $0.7{\mu}M$ OA, the number of chromosomes were increased one in E and F group, two in G+Y group compared with control group. In treated with $1.5{\mu}M$ OA, the increasing number of chromosome was one in E and F group. In treated with $3{\mu}M$ OA, E and F group was increased one and G+Y group were increased two chromosomes compared with control group. But in treated with $6{\mu}M$ OA, the number of chromosome in G+Y group was decreased one. 2) $K_{562}$ tumor cell line treated with OA showed Philadelphia-Chromosome in the long arm of the G group karyotype chromosome. The rate of chromosome aberration in $K_{562}$ tumor cell-line treated with OA was 77% in $0.7{\mu}M$ OA group, 71% in $1.5{\mu}M$ OA group, 82% in $3{\mu}M$ OA group and 94% in $6{\mu}M$ OA group respectively. The rate of chromosome aberration of $K_{562}$ tumor cell-line treated with OA was high in the high dose level of OA, and chromosome aberration of $K_{562}$ tumor cell-line treated with OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype. As a result of this study, the toxicity of OA showed deletion, minute, dicentric-chromosome and translocation in the long arm of the C-group karyotype, and then, the toxicity of OA resulted in the damage to RNA and protein synthesis in $K_{562}$ tumor cell-line, and the C-group karyotype of $K_{562}$ tumor cell-line was target of the toxicity of OA.

• Journal of Acupuncture Research
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• v.15 no.2
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• pp.61-79
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• 1998

In order to study the immunohistochemical effects of herbal drug-acupuncture of Radix paeoniae lactiflorae on gastric ulcer induced by HCl-aspirin in rats. this experiment was done by herbal drug-acupuncture to Wisu($B_{21}$). Chung-Wan($CV_{12}$), Chok-Samni($S_{36}$) loci to measure histological features of ulcer lesion, the change of numbers of parietal cell, chief celI, gastrin and somatostatin-immunoreactive cell. The obtained results were as follows; 1. The ulcerative lesions of gastric mucosa were decreased to WiSU($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 2. In the numbers of parietal cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 3. In the numbers of chief cell, the most remarkable increase was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 4. In the numbers of gastrin-immunoreactive cell, the most remarkable decrease was observed to Wiu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups. 5. In the numbers of somatostatin-immunoreactive cell, the most remarkable decrease was observed to Wisu($B_{21}$) loci followed by Chung-Wan($CV_{12}$) and Chok-Samni($S_{36}$) loci compared to the control groups.

• The Journal of Korean Obstetrics and Gynecology
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• v.20 no.3
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• pp.13-34
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• 2007

Purpose: To evaluate the effect of administration of Guibitang on ovarian functions and differential gene expressions related cell viabilities caspase-3, MAPK and MPG in female mice. Methods: We administered the Guibitang to 6-week-old female ICR mice for 4, 8, or 12 days. Then, the female mice were injected PMSG and hCG for ovarian hyperstimulation. The mice divided into 3 different groups for each experiment. To compare the differences, we set a control group treated with plain water at the same volume by the same way. Results: administration of Guibitang, the mean number of total ovulated oocytes and the number of morphologically normal oocytes increased significantly compared to control group. And the rates of blastocyst formation from 2-cell stages alsa increased significantly compared to control group. Capase-3 gene expression which is known to maker gene for cell apoptosis were significantly lower than that of control group. And MAPK and MPG gene expressions for cell viability and DNA repair were same that of control group. Conclusion: From our results suggested that the medication of Guibitang has beneficial effect on reproductive functions of female mice via prevention of cell apoptosis and DNA damaging and promotion of cell proliferation.

• THE JOURNAL OF KOREAN ORIENTAL ONCOLOGY
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• v.4 no.1
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• pp.89-110
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• 1998

Yookmijihwangtang has been widely used oriental herb prescriptions, which is healing some discuss that come from insufficiency of innate essence and deficiency of kidney Ki. The meaning of healing discusses tonification of insufficient innate essence and insufficient kidney Ki can be regarded as reinforcement of wholely power of keeping homeostasis, that is correlated with immuno-responsibility which protects subject from outer antigen to keep normal vital condition. This study was aimed to investigate correlative effects of Yookmijihwangtang water abstract on the RBC, WBC, blood CD4+ T helper cell count, blood testosterone, blood cAMP and blood cortisol. 40 Sprague-Dawley male rats were divided into 5 groups(Normal, Control, Sample I, Sample II, Sample III), 6 animals in every group. Normal group was not treated anything, control group was administrated normal saline in the same dosage of Sample I. 3 Sample groups were received some of Yookmijihwangtang water abstract at one time per 24 hours during 5 days in different dosage. Sample I(1/310pack/ml), Sample II(1/62pack/ml), Sample III(1/2.4pack/ml). After finishing treatment, all experimental subjects were killed for blood sample on RBC, WBC, blood CD4+ T helper cell count, spleen CD4+ T helper cell count, axillary lymph node CD4+ T helper cell count. blood cAMP, blood testosterone and blood cortisol. The results were as follows; RBC and WBC were increased in all sample groups. Blood CD4+ T helper cell count(CD4+ T cell count in the blood/whole lymphocyte count in the blood ${\times}100%$) was Normal $46.17{\pm}5.88$, Control $44.50{\pm}4.37$, Sample I $53.00{\pm}2.28$, Sample II $53.83{\pm}3.87$, Sample III $52.17{\pm}2.93$. By the 95% Duncan ANOVA all experimental groups(sample I, Sample II, Sample III) showed slight significant difference from Normal and Control. Blood cAMP(nmol/l) were Normal $1.12{\pm}0.17$, Control $1.16{\pm}0.32$, Sample I $0.46{\pm}0.07$, Sample II $0.44{\pm}0.04$, Sample III $0.54{\pm}0.04$. All experimental groups were singificantly different from both Normal and Control groups(p<0.05). Blood cortisol(nl/ml) were Normal $100.00{\pm}2.00$ Control $90.00{\pm}4.00$, Sample I $440.00{\pm}5.00$, Sample II $520.00{\pm}40.00$, Sample III $470.00{\pm}7.00$. Blood cortisol of all experimental groups were significantly increased(p<0.05). The results suggest that Yookmijihwangtang water abstract could be administrated to patients who have some diseases insufficient essence.

• YAKHAK HOEJI
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• v.46 no.1
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• pp.18-23
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• 2002

Ailanthus altissima has been used to settle an upset stomach, to alleviate a fever and as an insecticide. We reported that the water extract of A. altissima induced apoptotic cell death in Jurkat T-acute Iymphoblastic leukemia cells. Here, we showed the dose-dependent inhibitions of cell viability by the extract, as measured by cell morphology. The cell cycle control genes are considered to play important roles in tumorigenesis. The purpose of the present study is also to investigate the effect of A. altissima on cell cycle progression and its molecular mechanism in the cells. The level of p21 protein was increased after treatment of the extract, whereas both Bcl-2 and Bax protein levels were not changed. These results suggest that A. altissima induces apoptotic cell death via p21-dependent signaling pathway in Jurkat cells which delete wild type p53. Gl checkpoint related gene products tested (cyclin D3, cyclin dependent kinase 4, retinoblastoma, E2Fl) were decreased in their protein levels in a dose-dependent manner after treatment of the extract Taken together, these results indicate that the increase of apoptotic cell death by A. altissima may be due to the inhibition of cell cycle in Jurkat cells.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.12
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• pp.6429-6433
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• 2012

To explore a possible new treatment for human ovarian cancer, we studied the effects of sodium valproate on the growth of the HO8910 human cell line. HO8910 cells were cultured in vitro and treated with different concentrations of sodium valproate. Cell proliferation, cell cycling, and apoptosis were measured by flow cytometry, cell morphology under a microscope, and expression levels of WWOX and P27 by Western blotting and RT-PCR. Tumor xenografts were established to determine in vivo effects of sodium valproate. Our results showed that cell proliferation was decreased with increasing concentration of sodium valproate, with features of cytoplasmic retraction and floating cells. Moreover, cell cycle analysis revealed a higher apoptosis rate and $G_0/G_1$ phase in the sodium valproate experimental group than in the control group. In addition, protein expression levels of WWOX and P27 were elevated. Importantly, sodium valproate decreased in vivo xenograft tumor burden and up-regulated WWOX and P27 expression in nude mice. In conclusion, sodium valproate might play a role in inhibition and control of ovarian cancer cell line HO8910 by inhibiting cell proliferation, interfering with the cell cycle and promoting apoptosis, so that it may be effective in the clinical treatment of ovarian cancer.

• Proceedings of the Korean Institute of IIIuminating and Electrical Installation Engineers Conference
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• 2007.11a
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• pp.279-285
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• 2007

Since the residential load is an AC load and the output of solar cell is a DC power, the photovoltaic system needs the DC/AC converter to utilize solar cell. In case of driving to interact with utility line, in order to operate at unity power factor, converter must provide the sinusoidal wave current and voltage with same phase of utility line. Since output of solar cell is greatly fluctuated by insolation, it is necessary that the operation of solar cell output in the range of the vicinity of maximum power point. In this paper, DC/AC converter is three phase PWM converter with smoothing reactor. And then, feedforward control used to obtain a superior characteristic for current control and digital PLL circuit used to detect the phase of utility line.

• 한국신재생에너지학회:학술대회논문집
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• 2007.06a
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• pp.288-291
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• 2007

Since the residential load is an AC load and the output of solar cell is a DC power, the photovoltaic system needs the DC/AC converter to utilize solar cell. In case of driving to interact with utility line, in order to operate at unity power factor, converter must provide the sinusoidal wave current and voltage with same phase of utility line. Since output of solar cell is greatly fluctuated by insolation, it is necessary that the operation of solar cell output in the range of the vicinity of maximum power point. In this paper, DC/AC converter is three phase PWM converter with smoothing reactor. And then, feedforward control used to obtain a superior characteristic for current control and digital PLL circuit used to detect the phase of utility line.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.21 no.12
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• pp.3154-3164
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• 1996

A major concern in a cellular mobile communication system is how to efficiently utilize the limited amount of frequencies. Various channel assignment methods and traffic sharing schemes have been proposed to improve system performance. However, these approaches suffer from complicated software and hardware requirements due to increased amount of traffic control In this paper, we propose a new method for improving system performance by controlling the powers of the set-up and voice channels of each cell site. We first show that the average number of blocked calls in a system is minimized when the traffic reates are made identical for all cell sites in a system. This result, together with the relationship between the channel powers and the service area, is used to determine the appropriate channel powers of each cell site. We also determine the upper limit on the channel power of each cell site considering co-channel interference and numerically show that the proposed method is effective in reducing the number of blocked calls without an excessive increase in the amount of system control.

• Proceedings of the KIPE Conference
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• 2002.07a
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• pp.754-758
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• 2002

In the fuel-cell electric vehicle system, the low-voltage output of unit fuel-cell demands a number of cells to be stacked In series to produce a DC link voltage which is high enough to drive the vehicle inverter system. However, this increases the complexity of the fuel-cell control system. This paper presents a design of high-efficiency boost converter employing the average current-mode control, which is able to convert a low voltage of a fuel-cell generator with a small number of unit cells to a stable and high DC link voltage for electric vehicle applications.