• Title/Summary/Keyword: cell compatibility

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Development of Hydrogen Flow Field Standard in Hydrogen Refueling Station (수소충전유량 현장교정시스템의 개발 )

  • WOONG KANG;JINWOO SHIN;SAENG-HEE LEE;BYUNG-RO YOON;UNBONG BAEK
    • Journal of Hydrogen and New Energy
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    • v.33 no.6
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    • pp.684-691
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    • 2022
  • Hydrogen fuel cell electric vehicles are typically refueled at a wide range of temperatures (-40℃ to 85℃) in the hydrogen refueling station in accordance with the worldwide accepted standard. Currently, there is no traceable method by which to verify and calibrate the hydrogen flowmeters to be used at hydrogen refueling stations except for a water calibration process as a conventional method. KRISS hydrogen field test standard based on the gravimetric principle was developed to verify the measurement accuracy of the mass flowmeter to be used at hydrogen refueling stations for the first time in Korea.

The Biocompatibility Evaluation of Resin-Based Root Canal Sealers (Resin-Based Root Canal Sealer의 생체 적합성 평가)

  • Kim, Hyoung-Sun;Chon, Seong-Min;Moon, Jhong-Hyun;Lee, Kwang-Won;Yu, Mi-Kyung
    • Journal of Dental Rehabilitation and Applied Science
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    • v.23 no.1
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    • pp.95-104
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    • 2007
  • I. Objective The primary requirement of an endodontic root canal sealer is the biologic compatibility, because they remain in close contact with living periapical tissues over a long period of time. The aim of this study was the evaluation of cytotoxicity and genotoxicity of resin-based root canal sealers, AH 26 and ADSEAL. II. Material & Methods In this study, human periodontal ligament cells, human oral cancer cells (KB) and mouse osteoblasts (MC-3T3-E1) were used. Specimens of AH26, ADSEAL were eluted with culture medium for 1, 3, 5 and 7 days. Cytotoxicity was evaluated by using tetrazolium bromide reduction assay (MTT assay) for mitochondrial enzyme activity and cell viability. Genotoxicity was evaluated by using alkaline single cell gel electrophoresis assay (Comet assay). Also cell apoptosis induced by AH 26 was detected by Hoechst33258 staining. III. Results AH 26 and ADSEAL exhibited cytotoxic effects in all investigated cell groups. Genotoxicity was also noted for both sealers in mouse osteoblasts (MC-3T3-E1). But, ADSEAL presented significantly low cytotoxicity and genotoxicity compared with AH 26. Cytotoxicity and genotoxicity induced by AH 26 resulted in apopotosis. IV. Conclusion Our results clearly indicate that the recently invented ADSEAL has better biocompatibility than another resin based root canal sealer, AH 26. However ideal root canal sealer should have not only biocompatibility but also satisfactory physico-chemical properties such as sealing ability and stability. Thus continuous studies and developments should follow.

Molecular Analysis of HLA-C Using Polymerase Chain Reaction-Sequence Specific Primers

  • Lee, Kyung-Ok;Hong, Sung-Hoi;Kim, Min-Jung;Park, Taek-Kyu;Kim, Yoon-Jung;Lee, Kyu-Pum
    • BMB Reports
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    • v.30 no.1
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    • pp.26-32
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    • 1997
  • Of all HLA class I molecules, HLA-C gene products are most poorly understood because they express at a low level on the cell surface compared to HLA-A and -B. In order to identify serologically detectable and undetectable HLA-C antigens, we have established a DNA-based tissue typing method for the HLA-C locus by PCR-SSP (polymerase chain reaction-sequence specific primers). Genomic DNA prepared from Iymphoblastoid 21 B-cell lines and 120 Korean individuals by proteinase K digestion and pheno/chloroform extractions have been typed by PCR-SSP (23 primer mixes were used). The PCR-SSP results of control cell lines were discrepant from serology in 1 case among 21 cases: Cw6 which was negative by serology but positive by PCR-SSP (cell line: MANIKA). Twenty four HLA-Cw "blank" antigens among fifty Korean individuals were completely determined by PCR-SSP DNA typing. HLA-Cw*0101 (15.3%), Cw*1401 (12.3%) and Cw*0701 (11.7%) alleles were frequently found in 120 Korean individual samples. In conclusion. the high level of discrimination for HLA-C alleles may prove useful and informative in the study of transplant survival, and identify the importance of allelic differences, not readily detectable by serology, on host and donor compatibility.

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Numerical Analysis of Electromagnetic Radiation Characteristics by High Voltage and General Cables for Fuel Cell Electric Vehicle (FCEV) (수소 연료전지 차량용 고전압 케이블과 일반 케이블에 의한 차량 전자파 방사 특성 수치해석 연구)

  • Lee, Soon-Yong;Seo, Won-Bum;Lim, Ji-Seon;Choi, Jae-Hoon
    • Journal of Hydrogen and New Energy
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    • v.22 no.2
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    • pp.152-160
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    • 2011
  • The electromagnetic characteristics of FCEVs (fuel cell electric vehicles) are much different from the existing combustion engine cars as well as hybrid, plug-in-hybrid, and pure electric vehicles due to the high voltage/current generated by a fuel cell stack which uses a compressed hydrogen gas reacted with oxygen. To operate fuel cell stack efficiently, BOP (Balance of Plant) which is consisted of many motors in water pump, air blower, and hydrogen recycling pump as well as inverters for these motors is essential. Furthermore, there are also electric systems for entertainment, information, and vehicle control such as navigation, broadcasting, vehicle dynamic control systems, and so on. Since these systems are connected by high voltage or general cables, EMC (Electromagnetic compatibility) analysis for high voltage and general cable of FCEV is the most important element to prevent the possible electric functional safety errors. In this paper, electromagnetic fields by high voltage and general cables for FCEVs is studied. From numerical analysis results, total time harmonic electromagnetic field strength from high voltage and general cables have difference of 13~16 dB due to ground effect by impedance matching. The EMI results of FECV at 10 m distance shows difference of 41 dB at 30 MHz and 54 dB at 230 MHz compared with only general cable routing.

Effectiveness of premarital screening program for thalassemia and sickle cell disorders in Ras Al Khaimah, United Arab Emirates

  • Salama, Rasha Aziz Attia;Saleh, Abeer Kamal
    • Journal of Genetic Medicine
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    • v.13 no.1
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    • pp.26-30
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    • 2016
  • Purpose: Genetic disorders can be prevented by basic public health measures and activities that focus primarily on education and approaches in Primary Health Care. Premarital screening is one such approach that can identify asymptomatic carriers of hemoglobinopathies and provide genetic counseling to couples for a healthy reproductive life. This study aimed to estimate the prevalence of beta thalassemia and sickle cell disorders in the adult population screened as a part of the United Arab Emirates Premarital Screening Program and to measure the effectiveness of the program in decreasing high-risk marriages in Ras Al Khaimah (RAK). Materials and Methods: A retrospective, population-based study was conducted at the RAK Primary Health Care Center, where the National Premarital Screening Program is implemented. The study included data collected from the premarital screening records of all couples who had applied for a marriage license during 2008-2015. Results: Of the 17,826 individuals screened during the studied period, 4.02% (717) were diagnosed as positive for hemoglobinopathies. The prevalence of beta thalassemia and sickle cell disorders among the total study population was 2.98% and 1.05%, respectively. The hemoglobinopathy trait was more prevalent than the disease. Among these 8,913 couples who were issued certificates for compatibility based on screening tests, 28 (0.31%) couples were declared high-risk (unmatched). Seventy percent of these unmatched couples reported consanguineous marriages. Conclusion: The program was successful in achieving its objective of identifying high-risk marriages. In spite of the counseling, however, all of the high-risk couples still married each other.

Evaluation of antibacterial activity and osteoblast-like cell viability of TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coating on titanium

  • Ji, Min-Kyung;Park, Sang-Won;Lee, Kwangmin;Kang, In-Chol;Yun, Kwi-Dug;Kim, Hyun-Seung;Lim, Hyun-Pil
    • The Journal of Advanced Prosthodontics
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    • v.7 no.2
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    • pp.166-171
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    • 2015
  • PURPOSE. The aim of this study was to evaluate antibacterial activity and osteoblast-like cell viability according to the ratio of titanium nitride and zirconium nitride coating on commercially pure titanium using an arc ion plating system. MATERIALS AND METHODS. Polished titanium surfaces were used as controls. Surface topography was observed by scanning electron microscopy, and surface roughness was measured using a two-dimensional contact stylus profilometer. Antibacterial activity was evaluated against Streptococcus mutans and Porphyromonas gingivalis with the colony-forming unit assay. Cell compatibility, mRNA expression, and morphology related to human osteoblast-like cells (MG-63) on the coated specimens were determined by the XTT assay and reverse transcriptase-polymerase chain reaction. RESULTS. The number of S. mutans colonies on the TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated surface decreased significantly compared to those on the non-coated titanium surface (P<0.05). CONCLUSION. The number of P. gingivalis colonies on all surfaces showed no significant differences. TiN, ZrN and $(Ti_{1-x}Zr_x)N$ coated titanium showed antibacterial activity against S. mutans related to initial biofilm formation but not P. gingivalis associated with advanced periimplantitis, and did not influence osteoblast-like cell viability.

High-Frequency Analysis Modeling of Hybrid Vehicle Battery (하이브리드 자동차 배터리의 고주파 해석 모델링)

  • Lee, Jae-Joong;Lee, June-Sang;Kim, Mi-Ro;Kweon, Hyck-Su;Nah, Wan-Soo
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.61 no.2
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    • pp.263-269
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    • 2012
  • In order to present that the electromagnetic compatibility standards following the frequency goes up which is based automotive electronics, in this paper, a hybrid/electric vehicle battery which reflects the frequency of the equivalent circuit model is introduced. By using this circuit modeling, the impedance characteristics can be analysed and an analyze of battery one cell is finished. Using this model, each different from the discharging situation, the discharge characteristic curve could be led. Basic theoretical approaches and measuring results through MATLAB and experimental validation of the EIS measurement equipment was used.

A Study on the SEU in the SRAM to proton Irradiation

  • Lho, Young-Hwan;Park, Bo-Kyun;Kim, Bong-Sun
    • 제어로봇시스템학회:학술대회논문집
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    • 2003.10a
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    • pp.2295-2297
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    • 2003
  • The major problem encountered in satellite design is EMI (Electro-Magnetic Interference) and EMC (Electro-Magnetic Compatibility). Here, our focus is on the effects of protons on the electronic system. The SEU (Single Event Upset) results from the level change of stored information due to photon radiation and temperature in the space and the nuclear power plant environment. The impact of SEU on PLD (Programmable Logic Devices) technology is most apparent in ROM/SRAM/DRAM devices wherein the state of storage cell can be upset. In this paper, a simple and powerful test techniques is suggested, and the results are presented for the analysis and future reference. The test results are compared with that of JPL test report. In our experiment, the proton radiation facility available at KIRAMS (Korea Institute of Radiological Medical Sciences) has been applied on a commercially available SRAM manufactured by Hynix Semiconductor Company.

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A multiple level set method for modeling grain boundary evolution of polycrystalline materials

  • Zhang, Xinwei;Chen, Jiun-Shyan;Osher, Stanley
    • Interaction and multiscale mechanics
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    • v.1 no.2
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    • pp.191-209
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    • 2008
  • In this paper, we model grain boundary evolution based on a multiple level set method. Grain boundary migration under a curvature-induced driving force is considered and the level set method is employed to deal with the resulting topological changes of grain structures. The complexity of using a level set method for modeling grain structure evolution is due to its N-phase nature and the associated geometry compatibility constraint. We employ a multiple level set method with a predictor-multicorrectors approach to reduce the gaps in the triple junctions down to the grid resolution level. A ghost cell approach for imposing periodic boundary conditions is introduced without solving a constrained problem with a Lagrange multiplier method or a penalty method. Numerical results for both uniform and random grain structures evolution are presented and the results are compared with the solutions based on a front tracking approach (Chen and Kotta et al. 2004b).

Analysis of CANDU-6 Transition Core Refuelled from 37-Element Fuel to CANFLEX-NU Fuel

  • Jeong, Chang-Joon;Lee, Young-Ouk;Suk, Ho-Chun
    • Proceedings of the Korean Nuclear Society Conference
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    • 1997.05a
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    • pp.77-82
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    • 1997
  • The CANDU-6 transition core refuelled from 37-element fuel to CANFLEX-NU fuel has been evaluated by an 100full power day time-dependent fuel-management simulation to find the core compatibility with the CANFLEX fuel loading. The simulation calculations for the transition core were carried out with the RFSP code, provided by the cell averaged fuel properties obtained from the POWDERPUFS-V code. The simulation results were compared with those of the current 37-element fuel loading only. The results show that the CANFLEX-NU fuel bundles will be compatible with the CANDU-6 reactor because the core physics characteristics of CANFLEX-NU fuel are very similar to those of the 37-element fuel bundle.

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