• Molecular & Cellular Toxicology
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• 제2권1호
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• pp.60-66
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• 2006

Methylmercury (MeHg), one of the heavy metal compounds, can cause severe damage to the central nervous system in humans. Many reports have shown that MeHg is poisonous to human body through contaminated foods and has released into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established so far. This study, using of suppression subtractive hybridization (SSH) method, was peformed to identify differentially expressed genes by MeHg in SH-SY5Y human neuroblastoma cell line. We prepared to total RNA from SH-SY5Y cells treated with solvent (DMSO) and $6.25\;{\mu}M\;(IC_{50})$ MeHg and performed forward and reverse SSH. Differentially expressed cDNA clones were screened by dot blot, sequenced and confirmed that individual clones indeed represent differentially expressed genes with real time RT-PCR. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences may provide an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as ubiquitous environmental pollutants.

• 대한약침학회지
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• 제25권2호
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• pp.88-100
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• 2022

Gastric cancer (GC) is a significant cause of cancer mortality which has led to focused exploration of the pathology of GC. The advent of genome-wide analysis methods has made it possible to uncover genetic and epigenetic fluctuation such as abnormal DNA methylation in gene promoter regions that is expected to play a key role in GC. The study of gastric malignancies requires an etiological perspective, and Helicobacter pylori (H. pylori) was identified to play a role in GC. H. pylori infection causes chronic inflammation of the gastric epithelium causing abnormal polyclonal methylation, which might raise the risk of GC. In the last two decades, various pathogenic factors by which H. pylori infection causes GC have been discovered. Abnormal DNA methylation is triggered in several genes, rendering them inactive. In GC, methylation patterns are linked to certain subtypes including microsatellite instability. Multiple cancer-related processes are more usually changed by abnormal DNA methylation than through mutations, according to current general and combined investigations. Furthermore, the amount of acquired abnormal DNA methylation is heavily linked to the chances of developing GC. Therefore, we investigated abnormal DNA methylation in GC and the link between methylation and H. pylori infection.

• 한국환경독성학회:학술대회논문집
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• 한국환경독성학회 2002년도 추계국제학술대회
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• pp.167-167
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• 2002

Methylmercury (MeHg), one of the heavy metal compound, can cause severe damage to the central nervous system in humans. Many reports have contributed MeHg poisoning to contaminated foods and release into the environment. Despite many studies on the pathogenesis of MeHg-induced central neuropathy, no useful mechanism of toxicity has been established. To find genes differentially expressed by MeHg in neuronal cell, we peformed forward and reverse suppression subtractive hybridization (SSH) method on mRNA derived from neuroblastoma cell line, SH-SY5Y treated with solvent (DMSO) and 6.25 uM (IC$\sub$50/) MeHg. Differentially expressed CDNA clones were sequenced and the mRNAs were re-examined on Northern blots. These sequences were identified by BLAST homology search to known genes or expressed sequence tags (ESTs). Analysis of these sequences has provided an insight into the biological effects of MeHg in the pathogenesis of neurodegenerative disease and a possibility to develop more efficient and exact monitoring system of heavy metals as common environmental pollutants.

• 한국의사학회지
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• 제22권1호
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• pp.57-67
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• 2009

The results of analyzing "Shanghanlun-Shanghan examples" of Song Dynasty Text and Liuwansu's writings in the pursuit of comprehending his views written in "Fire-Heat" & "Warm disease" -are as follow. 1.He introduced Exterior-Interior Shanghan and Latent in the Winter & recurrent in the Spring Shanghan concept in the Febrile disease pathology as the link and the bifurcation point. His perception on Febrile disease encompassed Shanghan and Febrile disease on the same plane but actually discriminated in the treatment and medication between the two actually. 2.He suggested Febrile disease pathology in the concept of Shanghan and Febrile disease, which is coherent in the formation of interior heat as the result of the invasion of the lung by warm pathogen described in Warm disease Wei, Qi, Ying and Xue Fen Syndrome differentiation. He expanded the concept of esoteric portal 玄府 of "Suwon Tiaojinglun", to that of the omni-present portal & way of Qi's all directions movement, which is in accordance of invasion of upper energizer 上焦 by warm or hot pathogen via mouth and nose as used in Warm disease three-energizer differentiation. 3.He recognized both exterior-interior febrile disease in the context of both exterior-interior contact cause disease. He prescribed pungent-cool exterior releasing medicinal similar to that of exterior-releasing treatment adopted in warm disease. As lily-talc powder百合散 introduced in "Golden chamber synopsis" dispells heat by promoting excretion of urine and defecation, it is regarded as the original formula of later ikwonsan yiyuansan 益元散.

• 수면정신생리
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• 제11권1호
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• pp.17-21
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• 2004

Nasal congestion is one of the most common symptoms of medical complaints. Snoring is caused by vibration of the uvula and the soft palate. Nasal obstruction may contribute not only to snoring and obstructive sleep apnea (OSA) but also impair application of continuous nasal positive airway pressure (CPAP), which is the most widely employed treatment for OSA. Total or near-total nasal obstruction leads to mouth breathing and has been shown to cause increased airway resistance. However, the exact role of the nasal airway in the pathogenesis of OSA is not clear and there is no consensus about the role of nasal obstruction in snoring and sleep apnea. Some reports have failed to demonstrate any correlation between snoring and nasal obstruction. On the other hand, opposing reports suggest that nasal disease may cause sleep disorders and that snoring can be improved after nasoseptal surgery. Reduced cross-sectional area causes increased nasal resistance and predisposes the patient to inspiratory collapse of the oropharynx, hypopharynx, or both. Discrete abnormalities of the nasal airway, such as septal deformities, nasal polyps, and choanal atresia and with certain mucosal conditions such as sinusitis, allergic rhinitis and inferior turbinate hypertrophy can cause snoring or OSA. Thus, these sources of nasal obstruction should be corrected medically or surgically for the effective management of OSA and adjunctive for CPAP.

• 대한한방체열의학회지
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• 제8권1호
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• pp.33-38
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• 2010

Purpose : Dam-eum and women about this disease is closely related to the fact that research is not developed encounter the report. The purpose of this disorder is associated with women with Dameum hew to evaluate the affects around Dongulbogam Search and compare some of the knowledge gained. are reported. Methods : Donguibogam focused on search and related papers were. Results : In Donguibogam Dam-eum(痰), Yoen(涎), Eum(飮) to distinguish, but the reason was that one, 痰飮 sap needed to nutritious the body, such as a material or normal for various reasons by the sap from the leaves to cause disease states may be the cause. Conclusion: Digestive, respiratory-related, but now the recognition of the Dameum changes in diet and lack of exercise, stress, lifestyle diseases due to climate change are increasing asa Dameum and women in the pathogenesis of disease high enough proportion of the treatment for Dameum Applications will be denied a lot of disease is considered.

• 대한한의학원전학회지
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• 제30권2호
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• pp.11-29
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• 2017

Objectives : Contemporary researches suspect that, contrary to the past belief, the understanding that the cause of warm pathogen lies in the upper portion of human body is an understanding that had been well-established even before Yetianshi. This new understanding now requires us to contemplate the process of theoretical development which this understanding, termed Onsasangsu, had taken within the boundary of the theory of warm pathogen. This paper aims to shed light on this within the framework that this is the emergence of a new theory of warm pathogen caused by a new understanding of warm pathogen. Methods : First, the theories of warm pathogen as developed by historical doctors were studied, and elements that seem to be related to the understanding of Onsasangsu were selected and studied to understand their theoretical characteristics. Furthermore, the paper studied what academic significance do these theories have on the development of the theory of warm pathogen. Results & Conclusions : Provided that the underlying assumption of Onsasangsu is that febrile diseases are caused through moutn and nose, the study showed that this understanding arose before the period of Qing Dynsasty from the need by many doctors to differentiate the pathogens of various diseases such as the disease of heat, febrile disease, and epidemic. The reason that these discussions could not have much impact on the study of febrile disease during the Qing Dynasty could be because they were not passed on down to the future generations, or because commonly held perspective was unable to accept criticisms.

• 동의생리병리학회지
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• 제29권4호
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• pp.281-288
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• 2015

This paper researches the features of blood stasis theory of Wangqingren, who wrote 『Yilingaicuo』 that greatly contributed in the development of blood stasis theory at Qing dynasty period. And the disease cause, disease mechanism of blood stasis and scope of modern diseases related with blood stasis are studied by research on clinical papers which used 33 prescriptions in 『Yilingaicuo』 in modern times. Research on the features of blood stasis theory of Wangqingren is proceeded by referring to the annotations of 『Yilingaicuopingyi』 and the papers which related with blood stasis from Korea and China. And clinical papers are searched in China Academic Journals(CAJ) of China National Knowledge Infrastructure(CNKI) to analyse the scope of modern diseases related with blood stasis. The features of blood stasis theory in 『Yilingaicuo』 expanded the range of existing theory. Wangqingren thought that chronic disease, weird disease, the disease of no effect from normal treatments were related with blood stasis. And he attached great importance to qi and blood and thought that the main pathogenesis of blood stasis was qi deficiency. And a lot of Astragalus membranaceus Bunge were combined in many prescriptions to reinforce qi. He also used different herbs according to the location of the disease. Musk and Allium fistulosum were used for the disease located at head or upper part of the patient's trunk. Bupleurum falcatum L., Aurantii Fructus Pericarpium and Platycodon grandiflorum A. De Candolle were used for the disease located at thorax. Cyperus rotundus L., Linderae Radix and Aurantii Fructus Pericarpium were used for the disease located at the stomach or below the costal angle. Foeniculi Fructus and Corydalis remota were used for the disease located at belly or lower part of the patient's trunk. Trogopterorum Faeces, myrrha, Cyperus rotundus L. and Cnidium officinale were used for the disease located at extremity or joint.

• BMB Reports
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• 제49권3호
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• pp.139-148
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• 2016

In the past decade, the incidence of type 2 diabetes (T2D) has rapidly increased, along with the associated cardiovascular complications. Therefore, understanding the pathophysiology underlying T2D, the associated complications and the impact of therapeutics on the T2D development has critical importance for current and future therapeutics. The prevailing feature of T2D is hyperglycemia due to excessive hepatic glucose production, insulin resistance, and insufficient secretion of insulin by the pancreas. These contribute to increased fatty acid influx into the liver and muscle causing accumulation of lipid metabolites. These lipid metabolites cause dyslipidemia and non-alcoholic fatty liver disease, which ultimately contributes to the increased cardiovascular risk in T2D. Therefore, understanding the mechanisms of hepatic insulin resistance and the specific role of liver lipids is critical in selecting and designing the most effective therapeutics for T2D and the associated co-morbidities, including dyslipidemia and cardiovascular disease. Herein, we review the effects and molecular mechanisms of conventional anti-hyperglycemic and lipid-lowering drugs on glucose and lipid metabolism.

• BMB Reports
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• 제54권6호
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• pp.295-304
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• 2021

Olfactory neuropathology is a cause of olfactory loss in Alzheimer's disease (AD). Olfactory dysfunction is also associated with memory and cognitive dysfunction and is an incidental finding of AD dementia. Here we review neuropathological research on the olfactory system in AD, considering both structural and functional evidence. Experimental and clinical findings identify olfactory dysfunction as an early indicator of AD. In keeping with this, amyloid-β production and neuroinflammation are related to underlying causes of impaired olfaction. Notably, physiological features of the spatial map in the olfactory system suggest the evidence of ongoing neurodegeneration. Our aim in this review is to examine olfactory pathology findings essential to identifying mechanisms of olfactory dysfunction in the development of AD in hopes of supporting investigations leading towards revealing potential diagnostic methods and causes of early pathogenesis in the olfactory system.