• Toxicological Research
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• 제27권1호
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• pp.15-18
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• 2011

The toxicities of phenanthrene (PH) and pyrene (PY) are less than benzo(a)pyrene (BaP), but both compounds are found in higher concentrations in the air, feed, and food. Most PAHs are metabolized to hydroxylated compounds by the hepatic cytochrome P450 monooxigenases system. Metabolites are excreted into urine and feces. We determined concentrations of PH, PY and BaP in muscle and hydroxylated metabolites, 3-OH-PH, 1-OH-PY, and 3-OH-BaP, respectively, in urine from dairy cattle (n = 24). We also evaluated the relationship between parent compounds in muscle and their metabolites in urine. Concentrations of PH and PY in muscle ranged from 0.7~4.8 ng/g ($1.8{\pm}1.7$) and 0.4~4.1 ng/g ($1.2{\pm}1.2$), respectively. Concentrations of 3-OH-PH and 1-OH-PY in urine ranged from 0.1~5.9 ng/ml ($2.9{\pm}3.7$) and 0.5~3.6 ng/ml ($1.9{\pm}2.3$), respectively. Correlation coefficient for PY concentration in muscle versus 1-OH-PY in urine was 0.657 and for PH concentration in muscle versus 3-OH-PH in urine was 0.579. Coefficient determination for PY and PH concentrations in muscle was 0.886 and for 1-OH-PY and 3-OH-PH in urine was 0.834. This study suggests that 1-OH-PY and 3-OH-PH could be used as biomarkers for PAHs exposure in dairy cattle.

• Asian-Australasian Journal of Animal Sciences
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• 제16권2호
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• pp.297-305
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• 2003

The family of lemnacae colloquially known as duckweed contains the world' smallest species of flowering plants (macrophytes). Aquatic and free-floating, their most striking qualities are a capacity for explosive reproduction and an almost complete lack of fibrous material. They are widely used for reducing chemical loading in facultative sewage lagoons, but their greatest potential lies in their ability to produce large quantities of protein rich biomass, suitable for feeding to a wide range of animals, including fish, poultry and cattle. Despite these qualities there are numerous impediments to these plants being incorporated into western farming systems. Large genetically determined variations in growth in response to nutrients and climate, apparent anti-nutritional factors, concerns about sequestration of heavy metals and possible transference of pathogens raise questions about the safety and usefulness of these plants. A clear understanding of how to address and overcome these impediments needs to be developed before duckweed is widely accepted for nutrient reclamation and as a source of animal feed.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2008년도 춘계학술발표회
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• pp.5-13
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• 2008

The aquatic fern Azolla spp. is of value as a bio-fertilizer for wetland paddy, and also can be used as an ideal feed for cattle, pigs and poultry. It is popular and cultivated widely in other countries like China, Vietnam, and the Philippines, but has yet to be taken up in Korea, in a big way. It fixes nitrogen as high as 3-5kg N per day, because it contains nitrogen fixing blue-green algae, Anabaena azollae. Azolla's ability to create a light-proof mat that suppresses other weeds has been used for centuries in rice production. Azolla spp. has also the capacity to take up the heavy metals ($75{\sim}100%$) and may be used as a bioaccumulator. Moreover, Azolla meal as an unconventional feed resource has a potential as a feedstuff for livestock.

• Journal of Animal Science and Technology
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• 제45권6호
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• pp.997-1006
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• 2003

본 연구는 우리나라에서 가축 장내발효에 의한 메탄배출 통계 기반을 구축하기 위해 수행되었다. 메탄 배출량을 추정하기 위해 가축은 소, 돼지, 가금, 면양, 산양으로 대분류하였고, 소는 한우와 젖소로 분류하여 각각 한우 송아지, 한우 비육우, 한우 번식우, 젖소 송아지, 젖소 비육우, 착유우로 세분류하였다. Tier 2 방법에 의한 메탄 배출계수는 각 세분류 집단의 가축의 생산성, 생체중, 도체중, 일당 증체량, 소화율을 근거로 하여 산출되었다. 한우의 배출계수는 39～49kg/head/year 정도로 IPCC에서 제시한 Tier 1의 47kg/head/year과 유사한 수준이었고, 젖소의 배출계수는 107 kg/head/year로 Tier 1 배출계수, 118kg/head/ year 보다 다소 낮게 나타났다. Tier 2 방법으로 추정한 가축 장내발효에 의한 메탄 배출량은 2001년, 총 126.8톤이었고 각 세분류별 메탄 배출량은 한우, 젖소, 돼지, 면양, 산양, 말에서 각각 61.70, 47.76, 13.08, 2.25, 0.17, 0.01 톤으로 나타났다. 본 연구에서 온실가스 배출추정 방법으로, Tier 1을 Tier 2로 대체하므로써 우리나라 가축 장내발효에 의한 메탄배출 통계의 정확성과 신뢰성이 개선된 것으로 사료된다.

• Asian-Australasian Journal of Animal Sciences
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• 제32권5호
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• pp.657-664
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• 2019

Objective: This study was performed to evaluate whether cold ambient temperature and dietary rumen-protected fat (RPF) supplementation affect growth performance, rumen fermentation, and blood parameters in Korean cattle steers. Methods: Twenty Korean cattle steers (body weight [BW], $550.6{\pm}9.14kg$; age, $19.7{\pm}0.13months$) were divided into a conventional control diet group (n = 10) and a 0.5% RPF supplementation group (n = 10). Steers were fed a concentrate diet (1.6% BW) and a rice straw diet (1 kg/d) for 16 weeks (January 9 to February 5 [P1], February 6 to March 5 [P2], March 6 to April 3 [P3], and April 4 to May 2 [P4]). Results: The mean and minimum indoor ambient temperatures in P1 ($-3.44^{\circ}C$, $-9.40^{\circ}C$) were lower (p<0.001) than those in P3 ($5.87^{\circ}C$, $-1.86^{\circ}C$) and P4 ($11.18^{\circ}C$, $4.28^{\circ}C$). The minimum temperature in P1 fell within the moderate cold-stress (CS) category, as previously reported for dairy cattle, and the minimum temperatures of P2 and P3 were within the mild CS category. Neither month nor RPF supplementation affected the average daily gain or gain-to-feed ratio (p>0.05). Ruminal ammonia nitrogen concentrations were higher (p<0.05) in cold winter than spring. Plasma cortisol concentrations were lower (p<0.05) in the coldest month than in the other months. Serum glucose concentrations were generally higher in colder months than in the other months but were unaffected by RPF supplementation. RPF supplementation increased both total cholesterol (p = 0.004) and high-density lipoprotein (HDL) concentrations (p = 0.03). Conclusion: Korean cattle may not be significantly affected by moderate CS, considering that the growth performance of cattle remained unchanged, although variations in blood parameters were observed among the studied months. RPF supplementation altered cholesterol and HDL concentrations but did not affect growth performance.

• 한국유기농업학회지
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• 제23권2호
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• pp.359-372
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• 2015

본 연구는 토양오염을 감소시키고 유기축산물의 생산에 기여할 수 있는 유기조사료를 효율적으로 생산하기 위해 대표적인 동계작물인 총체 밀에 콩과 사료작물의 혼파와 발효우분을 시용하여 생산성과 사료가치를 평가하고, 토양의 지력 향상과 가축분뇨의 자원화를 위한 단위면적당 유기한우 사육능력을 추정하고자 하였다. 콩과 사료작물의 혼파에 따른 2년(2012~2013년) 평균 총체 밀의 건물, 조단백질 및 TDN 수량은 6.10~7.70, 0.59~0.76 및 3.84~4.74톤/ha으로 단파로 재배했을 경우의 4.07, 0.35 및 2.54 톤/ha 보다 유의하게 증가하였으나 혼파구 간에는 차이가 없었고, 발효우분의 질소시용수준이 증가함에 따라 총체 밀의 평균 건물, 조단백질 및 TDN 수량은 100~150 kg N/ha (7.24~7.59, 0.67~0.73 및 4.43~4.69톤/ha)가 무비구(5.96, 0.57 및 3.71톤/ha)에 보다 유의하게 증가하였다(p<0.05). 총체 밀의 조단백질 함량은 콩과사료작물의 혼파구에서 평균 9.83~10.03%로 총체 밀 단파구의 8.37%보다 유의하게 높았고 ADF 함량은 헤어리 베치 혼파구 그리고 RFV는 사료용 완두 혼파구가 다른 처리구 보다 유의하게 높았으며(p<0.05), 발효우분의 질소시용수준에 따른 조단백질 함량은 9.17~9.52%로 처리수준 간 차이가 없었고 ADF 함량은 시용수준이 100 kg N/ha 까지 증가함에 따라 유의하게 증가하였고 TDN 함량은 반대로 유의하게 감소하였다(p<0.05). 콩과 사료작물의 혼파에 따른 총체 밀에 의한 유기한우 사육능력(체중 450 kg, 400 g 일당증체 목표)은 평균 3.99~4.20두로 단파구(평균 2.21두) 보다 유의하게 증가하였고. 발효우분 시용수준이 증가됨에 따라 질소 수준 100 kg/ha(평균 3.68두)까지 무비구(평균 3.09두) 보다 총체 밀에 의한 유기한우 사육능력도 유의하게 증가하였다(p<0.05). 이상의 결과를 종합해 볼 때, 총체 밀 재배 시 콩과 사료작물의 혼파 하였을 경우에는 단파재배 보다 발효우분의 질소시용수준을 ha 당 50 kg 이상 줄이면서도 건물수량을 포함한 생산성과 유기한우 사육능력 증대는 물론 조단백질 함량 등 사료가치를 향상을 시켜 한우사양 시 조사료자원의 다양화 뿐 아니라 저투입에 의한 양질의 조사료 생산으로 유기축산물 생산에 크게 기여하리라 사료된다.

• 한국환경과학회지
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• 제22권9호
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• pp.1235-1239
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• 2013

The effects of probiotic additions to feed and manure on temperature, humidity and carbon dioxide ($CO_2$) emissions in Hanwoo manure during summer (4 weeks) were evaluated. Fifteen Hanwoo (24-mo-old, $580{\pm}20kg$) were housed in individual pens ($5{\times}8m$) and randomly assigned to 1 of 3 treatments (n = 5 cattle per treatment). Hanwoo were fed experimental rations as follows: control (10 kg roughage + 2 kg concentrate); T1 (10 kg roughage + 2 kg concentrate, 2% probiotics on as-fed basis); and T2 (10 kg roughage + 2 kg concentrate, 2% probiotics on as-fed basis + 7 kg probiotics as top-dressing on the surface of Hanwoo manure). In comparison to the control, the addition of probiotics to feed or feed and manure had an effect (P < 0.05) on temperature and humidity over the 4 weeks, except for humidity at 0 weeks. The only significant difference (P < 0.05) observed in $CO_2$ emission was among all treatments at 3 and 4 weeks (but not at 0 through 2 weeks). These results indicated that use of probiotics as feed and manure additives did not have a significant effect on environmental parameters.

• 한국지역사회생활과학회지
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• 제23권2호
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• pp.129-136
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• 2012

Dioxins are chemical substances that are not properly decomposed degradation under natural photochemical, biological and chemical conditions in the environment, and bio-accumulate through the food chain, so pose a risk of causing adverse effects to human health and the environment. Ninety seven percent of human exposure to dioxins comes through meat, fish, dairy consumption and so on. Pork is the largest proportion meat aspect of daily intake in the Korean food. Pigs are three times more than Cattle in terms of breeding population and production amount in the world, and the amount is increasing every year. Therefore, it is necessary to monitor the exposure level of dioxins in pig's body. Also, it is required to evaluate the presence of these chemical substances in the pig's feed grain such as corn, soybeans, and tallow. The purpose of this study was to evaluate PCDD/Fs in pig's feed to search the origin of PCDD/Fs. Feed samples obtained were wheat from East Europe, corn from South America and America, soybean meal from Korea, America, South America and India and tallow from Korea. The preparation of samples was based on the EPA method 1613. Instrumental analysis was based on the use of high resolution gas chromatography coupled to high resolution mass spectrometry (HRGC/HRMS). The concentrations of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 1,2,3,7,8-pentachlorodibenzo-p-dioxin (PCDD), which are the most toxic potent dioxins, were not observed in any of the samples. However OCDD was detected in most of the samples. The TEQs in the feed samples were ranged from 0 pg TEQ/g to 0.09841 pg TEQ/g. Especially, the high level of TEQs was observed in the samples of wheat from East Europe and soybean meal from Korea.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.322-322
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• 2022

Hay and haylage as forage are increasing in preference for cattle feed. Triticale (X Triticosecale Wittmack) is growing up as a forage crop due to its high production and feed value in South Korea. It is difficult to determine the harvesting stage of triticale for producing hay or haylage with the highest forage value and quality because feed and nutritional value by growth stages of triticale were not known in South Korea yet. In this study, we analyzed forage values, pH, and contents of organic acid, to confirm feed value and quality by growth stages of triticale. The triticale 'Joseong' was sown in the spring of 2022. The sample was prepared in 5 stages: seedling stage, booting stage, heading stage, 10 days after heading, and 20 days after heading. The triticale was dried under 10% and to about 50% moisture for hay and haylage respectively, and then both triticales were fermented anaerobically at room temperature for 40 days. The pH value in all hay and haylage became lower as the triticale was grown up, although the pH value of all triticale hays and haylages ranged between 7.05-5.68. The content of lactic acid in all triticale hays was almost meaningless. The contents in the haylage of the seedling stage were the highest (2.39%) among the haylages, even though those of 20 days after the heading stage (1.67%) were the lowest, which showed a similar tendency with pH value. Conclusionally, the results implied that the feed value and quality of triticale haylage were affected by harvesting time but not hay.

• Asian-Australasian Journal of Animal Sciences
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• 제25권10호
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• pp.1466-1472
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• 2012

A bacterial isolate derived from soil samples near a cattle farm was found to display extracellular phytase activity. Based on 16S rRNA sequence analysis, the strain was named Bacillus sp. T4. The optimum temperature for the phytase activity toward magnesium phytate (Mg-$InsP_6$) was $40^{\circ}C$ without 5 mM $Ca^{2+}$ and $50^{\circ}C$ with 5 mM $Ca^{2+}$. T4 phytase had a characteristic bi-hump two pH optima of 6.0 to 6.5 and 7.4 for Mg-$InsP_6$. The enzyme showed higher specificity for Mg-$InsP_6$ than sodium phytate (Na-$InsP_6$). Its activity was fairly inhibited by EDTA, $Cu^{2+}$, $Mn^{2+}$, $Co^{2+}$, $Ba^{2+}$ and $Zn^{2+}$. T4 phytase may have great potential for use as an eco-friendly feed additive to enhance the nutritive quality of phytate and reduce phosphorus pollution.