• 한국영양학회:학술대회논문집
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• 한국영양학회 2003년도 추계학술대회 및 정기총회
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• pp.90-90
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• 2003

• 대한약학회:학술대회논문집
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• 대한약학회 2000년도 춘계총회 및 학술대회
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• pp.104.2-104.2
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• 2000

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.1
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• pp.106.1-106.1
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• 2002

• BMB Reports
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• 제37권3호
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• pp.325-329
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• 2004

Oxidation of catecholamines may contribute to the pathogenesis of Parkinson's disease (PD). The effect of the oxidized products of catecholamines on the modification of Cu,Zn-superoxide dismutase (SOD) was investigated. When Cu,Zn-SOD was incubated with the oxidized 3,4-dihydroxyphenylalanine (DOPA) or dopamine, the protein was induced to be aggregated. The deoxyribose assay showed that hydroxyl radicals were generated during the oxidation of catecholamines in the presence of copper ion. Radical scavengers, azide, N-acetylcysteine, and catalase inhibited the oxidized catecholamine-mediated Cu,Zn-SOD aggregation. Therefore, the results indicate that free radicals may play a role in the aggregation of Cu,Zn-SOD. When Cu,Zn-SOD that had been exposed to catecholamines was subsequently analyzed by an amino acid analysis, the glycine and histidine residues were particularly sensitive. These results suggest that the modification of Cu,Zn-SOD by oxidized catecholamines might induce the perturbation of cellular antioxidant systems and led to a deleterious cell condition.

• BMB Reports
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• 제29권2호
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• pp.142-145
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• 1996

To investigate the cause of increased plasma catecholamine levels in liver disease, catechol-O-methyltransferase (COMT), which provides a major route of catabolism for circulating catecholamines, was studied under the cholestasis induced by mechanical biliary obstruction in rats. Monoamine oxidase (MAO) activity and the $K_m$ and $V_{max}$ values for both enzymes were also measured. Cytosolic, microsomal, and mitochondrial COMT activities in the cholestatic liver were significantly decreased throughout the experiment. Microsomal, and mitochondrial MAO activity in the cholestatic liver were also significantly decreased. Vmax values of COMT and MAO were lower. Serum COMT and MAO activities were detected after CBD ligation. These results indicate that plasma catecholamine levels are increased in liver disease due to decreased hepatic degradation of catecholamines by decreased activities of COMT and MAO. The decreased activity of these enzymes is caused by decreased biosynthesis and by flowage into the blood from the damaged hepatocyte.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 1994년도 춘계학술대회 and 제3회 신약개발 연구발표회
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• pp.284-284
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• 1994

Pentazocine은 opioid 수용체에 대한 흥분작용과 길항작용을 겸유한 opioid계 약물로 알려져 있다. 본 연구에서 흰쥐 적출 관류부신 으로 부터 pentazocine의 catecholamine (CA) 분비작용을 관찰하여 그 기전을 규명하고 또한 다른 opioid의 작용과 비교하여 얻어진 결과는 다음과 같다. Pentazocine (30-300$\mu\textrm{g}$)을 부신정맥내에 주사하였을때 현저한 용량 의존성의 CA 분비 작용을 나타내었다. Pentazocine의 이러한 CA 분비작용은 chlorisondamine ($10^{-6}$M), naloxone (1.22 $\times$ $10^{-7}$M), morphine (1.73 $\times$ $10^{-5}$M). enkephalin (9.68 $\times$$10^{-6}$M), nicardipine ($10^{-6}$M) 및 TMB-8 ($10^{-5}$M)등의 전처치로 뚜렷이 억제되었으나 pirenzepine (2 $\times$ $10^{-6}$M)의 전처치에 의해서는 영향을 받지 않았다. $Ca^{++}$-free Krebs 용액으로 30분간 관류한 후에 pentazocine의 CA 분비작용은 현저한 감소를 나타내었다. Pentazocine (1.75 $\times$ $10^{-4}$M)을 20분간 관류시킨 후에 ACh (5.32 $\times$ $10^{-3}$M)과 DMPP ($10^{-4}$M)에 의한 CA 분비작용이 의의있게 감약되었다.

• 산업공학
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• 제16권2호
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• pp.149-155
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• 2003

The focus of this study is to investigate how personal display systems - a VDU (Visual Display Unit) and an HMD (Head Mounted Display) physiologically affect the body in virtual environments, and to evaluate differential effects of using the VDU and the HMD on physiological responses to mental stressful tasks (virtual reality flight simulation). As physiological variables, autonomic measures (heart rate, blood pressure), immune cells (leukocyte, neutrophil, lymphocyte), and hormones (catecholamine) were measured before and after experiments. Physiological data were measured in order to compare a level of mental stress on the VDU and the HMD. Increments in blood pressure (systolic (p<0.05), diastolic (p<0.1)), norepinephrin (catecholamine) (p<0.005), and neutrophils (p<0.2) of the group using the HMD showed a significant difference with the group using the VDU. Although, the heart rate was not statistically significant between two environments, differences of them quietly increased on the HMD more than on the VDU.

• Clinical and Experimental Reproductive Medicine
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• 제18권2호
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• pp.237-240
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• 1991

Cortisol, epinephrine and norepinephrine concentration in the antecubital and internal spermatic vein were measured and compared each other in 22 varicocele patients to investigate a possible toxic role of these materials to cause impairment of the spermatogensis. The results were as follow. 1) There were no significant differences of cortisol, epinephrine and norepinephrine concentrations between in the internal spermatic vein and in the peripheral vein, 2) Also in 6 patients who showed the abnormal findings on semen analysis and 7 patients who showed the impaired spermatogenesis on testicular biopsy, no significant differences of cortisol epinephrine and norepinephrine concentration were found between in the peripheral vein and in the internal spermatic vein. Therefore, cortisol, epinephrine and norephrine are not regarded as toxic materials responsible for the impairment of spermatogenesis in varicocele.

• 약학회지
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• 제36권3호
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• pp.285-290
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• 1992

After rats were exposed to 5,000 ppm carbon monoxide for 30 minutes, the amounts of catecholamine neurotransmitters in stratum were measured using high performance liquid chromatograph equipped with electrochemical detector. The concentration of dopamine in stratum was significantly decreased after carbon monoxide intoxification, but those of dihydroxyphenylacetic acid, norepinephrine, and epinephrine was not changed. However the pretreatments of Ginseng total saponin and panaxatriol saponin increased the concentrations of dopamine and its acidic metabolites (DOPAC and HVA). Ginseng total saponin also increased the concentrations of norepinephrine and epinephrine. Similar results were obtained from aged rats.

• 대한화학회지
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• 제35권3호
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• pp.219-225
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• 1991

고도로 효율적인 분석방법인 모세관 전기영동 시스템을 최적의 감도를 갖는 상태의 기기로 국내에서 제작하였다. 50 ${\mu}m$ 내경을 가진 모세관을 완충용액으로 채워준 후 모세관의 양끝에 20∼35 kV의 전압을 걸어주는 이 분석방법은 20분 이내에 10 nl 이하의 소량의 시료를 이론단수가 20∼50만 정도가 되도록 분리할 수 있는 매우 좋은 분리능으 가지고 있다. 모세관 전기영동법의 분리능과 분리도를 증가시키는데 영향을 주는 요소들을 알아보기 위하여 catecholamine과 adenine 유도체를 분석하였다.