• Microbiology and Biotechnology Letters
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• v.36 no.3
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• pp.240-245
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• 2008

Aloe vera extract was fermented by Lactobacillus casei. The ability of fermented Aloe vera (FAV) as an antioxidant to protect against $CCl_4$-induced oxidative stress and hepatotoxicity in rats was investigated. The rats were administered orally with various doses of FAV with 50, 100 mg/kg for 14 consecutive days. For this study, we not only tested activity of various plasma enzymes (AST, ALT), which are used as indicators of liver disease, but also checked those change of liver components such as superoxide dismutase and catalase activity. Pretreatment of FAV for two weeks significantly reduced the elevated plasma enzyme activities induced by $CCl_4$. Pretreatment of FAV also restored the hepatic enzyme, malonedialdehyde (MDA) formation. The results indicate that FAV has a protective effect against acute hepatotoxicity induced by the administration of $CCl_4$ in rats, and that the hepatoprotective effects of FAV may be due to both the inhibition of lipid peroxidation and the increase of antioxidant activity.

• YAKHAK HOEJI
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• v.51 no.4
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• pp.285-290
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• 2007

The oxidative stress causes many diseases like cancer, aging, cardiovascular disease, degenerative neurological disorders (Parkinson’s disease, and Alzheimer's disease) by damage of cell membrane, protein deformation, and damage of DNA due to the oxidation of lipid of cell membrane, protein of tissue or enzyme, carbohydrate, and DNA. It is caused by the reactive oxygen species (ROS) that is produced in the metabolic process of oxygen in cell. The superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) in cell systemize the antioxidative enzymes to control the oxidative stress. In this research, it is measured that the survival rate of cell by the typical isoflavonoid of daidzein or genistein, activity of antioxidative enzyme, and ROS level, in order to study the effect of isoflavonoid over the ROS production in cell and antioxidative system. As the similar action of the isoflavonoid with the estrogen is examined, women are encouraged to get bean. In view of this trend, it is very important to find out a combination medicine that lowers the oxidative stress caused by the daidzein in the ovarian cell. In the combined treatment of the typical antioxidant of vitamin C to oxidative stress which induced by daidzein recover the control level particularly lowering the ROS in cell by 30%. However, it made no effect in the combined treatment with genistein. Therefore, the research took the combination effect of daidzein with vitamin C in order to check it effect over the antioxidative system. In conclusion, it was disclosed that the oxidative stress caused by daidzein is related to the lowering activity of SOD, and the specific combination effect of daidzein with vitamin C is related to the recovery of SOD activity.

• 한국생물공학회:학술대회논문집
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• 2001.11a
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• pp.189-192
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• 2001

We have investigated the effects of abiotic and biotic stresses on leaf senescence using transgenic tobacco plants, in which cellular contents of polyamines were increased by introducing the genes of polyamine and ethylene biosynthesis in sense or antisense orientation. These transgenic plants showed accumulations of polyamines at higher levels than were found in wild-type. Stress-induced senescence was attenuated in transgenic plants cpmpared with wild-type plants, in terms of total chlorphyll loss and phenotypic changes after oxidative stress of hydrogen peroxide($H_2O_2$), high salinity, acid stress (pH3.0), ABA and fungal pathogen(phytophothora parasitica pv.Nicotianae). Transcripts for antioxidant enzyme, glutathionine-S-transferase and catalase, were also more abundant in transgenic plants than wild-type plants. These result suggested that higher expression of those genes caused a broad-spectrum resistance to abiotic stress/biotic stress. These phenomena indicate that polyamines may play an important role in contributing to the antioxidant defense function in plants. Our findings suggest that facilitate the improvement of stress tolerance of crop plants.

• Journal of Food Hygiene and Safety
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• v.33 no.1
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• pp.77-82
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• 2018

This study was carried out to investigate the effect of natural Eriobotrya japonica Lindl. vinegar on the liver protective effect of animals exposed to carbon tetrachloride. Eriobotrya japonica Lindl. vinegar (200 mg/kg) was administered at the same time for 28 days, and hepatotoxicity was induced by intraperitoneal injection of carbon tetrachloride on the $29^{th}$ day. The aspartate aminotransferase and alanine aminotransferase levels were significantly decreased (p < 0.001) and the superoxide dismutase and catalase activities were significantly increased (p < 0.001) in the Eriobotrya japonica Lindl. vinegar group compared to the control group. Histopathological observations showed that the Eriobotrya japonica Lindl. vinegar showed hepatic cell structure similar to normal group, and these results showed that it had an effect of suppressing and protecting the damage of liver cell. Therefore, Eriobotrya japonical Lindl. vinegar is considered to be a healthy functional food of the liver.

• Toxicological Research
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• v.32 no.2
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• pp.123-132
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• 2016

The impact of mobile phone (MP) radiation on the brain is of specific interest to the scientific community and warrants investigations, as MP is held close to the head. Studies on humans and rodents revealed hazards MP radiation associated such as brain tumors, impairment in cognition, hearing etc. Melatonin (MT) is an important modulator of CNS functioning and is a neural antioxidant hormone. Zebrafish has emerged as a popular model organism for CNS studies. Herein, we evaluated the impact of GSM900MP (GSM900MP) radiation exposure daily for 1 hr for 14 days with the SAR of 1.34W/Kg on neurobehavioral and oxidative stress parameters in zebrafish. Our study revealed that, GSM900MP radiation exposure, significantly decreased time spent near social stimulus zone and increased total distance travelled, in social interaction test. In the novel tank dive test, the GSM900MP radiation exposure elicited anxiety as revealed by significantly increased time spent in bottom half; freezing bouts and duration and decreased distance travelled, average velocity, and number of entries to upper half of the tank. Exposed zebrafish spent less time in the novel arm of the Y-Maze, corroborating significant impairment in learning as compared to the control group. Exposure decreased superoxide dismutase (SOD), catalase (CAT) activities whereas, increased levels of reduced glutathione (GSH) and lipid peroxidation (LPO) was encountered showing compromised antioxidant defense. Treatment with MT significantly reversed the above neurobehavioral and oxidative derangements induced by GSM900MP radiation exposure. This study traced GSM900MP radiation exposure induced neurobehavioral aberrations and alterations in brain oxidative status. Furthermore, MT proved to be a promising therapeutic candidate in ameliorating such outcomes in zebrafish.

• Korean Journal of Environmental Biology
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• v.19 no.3
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• pp.205-210
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• 2001

To determine the effect of low dose gamma radiation on the germination and enzyme activities, seeds of Chinese cabbage (Brassica compestris L. cv. Hanyoreum) and radish (Raphanus sativus L. cv. Chungsukoungzoung) were irradiated at the dose of 2-50 Gy. The germination rate of irradiation group was higher than that of the control. Especially it was highest at the early stage. The germination rate of Chinese cabbage was high at 2 Gy and 8 Gy irradiation group and that of radish was high at 2 Gy, 6 Gy and 10 Gy irradiation group. Growth of both seedlings of Chinese cabbage and radish increased positively in low dose irradiation group. The height of Chinese cabbage was noticeably high at 4 Gy and 10 Gy irradiation group and that of radish at 6 Gy irradiation group. The protein contents of seedlings from seeds irradiated with the low dose gamma radiation was higher than the control, especially at the early stage. The enzyme activities of seedlings from seeds irradiated with the low dose gamma radiation was high at 4 Gy and 10 Gy irradiation group. These results suggest that the germination, growth and enzyme activities of old vegetable seeds could be promoted by the low dose gamma radiation.

• Environmental Analysis Health and Toxicology
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• v.20 no.4 s.51
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• pp.287-296
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• 2005

아연은 다양한 독성 물질로부터 유도된 아폼토시스를 저해하는 것으로 알려져 있으나 이 기전에 대해서는 명확히 밝혀지지 않았다. 본 연구에서는 인간 유방암 세포 MCF-7에 카드뮴을 처리하였을 때 유도되는 아폼토시스에 대한 아연의 저해효과를 살펴보았다. 아연의 아폼토시스 저해 효과는 DNA분절현상, 핵의 쪼개짐 그리고 caspase-9의 발현을 통하여 확인하였다. 또한 아연의 아폼토시스 저해효과가 카드뮴에 의한 산화적 스트레스와 관련이 있는지 확인하기 위하여 활성산소인 peroxide의 농도를 세포내에서 측정하였다. 나아가 superoxide dismutase (SOD), catalase (CAT) 그리고 glutahion redurtase (CR)같은 활성산소에 대한 인체내 방어기작으로 작용하는 항산화 효소의 활성을 측정하였다. 본 연구를 통해 아연이 카드뮴에 의해 생성된 세포내의 활성산소의 양을 감소시키고 항산화 효소를 회복시키는 기전이 카드뮴에 의한 아폼토시스를 저해하는 한 요인으로 사료되어진다.

• Journal of Microbiology and Biotechnology
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• v.20 no.1
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• pp.88-93
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• 2010

Superoxide dismutase (SOD), glutathione peroxidase (GPX), and catalase (CAT) play crucial roles in balancing the production and decomposition of reactive oxygen species (ROS) in living organisms. These enzymes act cooperatively and synergistically to scavenge ROS. In order to imitate the synergism of these enzymes, we designed and synthesized a novel 32-mer peptide (32P) on the basis of the previous 15-mer peptide with GPX activity and a 17-mer peptide with SOD activity. Upon the selenation and chelation of copper, the 32-mer peptide was converted to a new Se- and Cu-containing 32-mer peptide (Se-Cu-32P) that displayed both SOD and GPX activities, and its kinetics was studied. Moreover, the novel peptide was demonstrated to be able to better protect vero cells from the injury induced by the xanthine oxidase (XOD)/xanthine/$Fe^{2+}$ damage system than its parents. Thus, this bifunctional enzyme imitated the synergism of SOD and GPX and could be a better candidate of therapeutic medicine.

• Environmental Analysis Health and Toxicology
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• v.22 no.2 s.57
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• pp.147-156
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• 2007

Six week-old ICR mice were divided into four groups including NC, RC, RR and RS, then they were injected with sun ginseng (RS), red ginseng (RR) and saline (RC) intraperitoneally as an amount of 60mg/kg body weight at 1 hour, 12 hours and 36 hours before the whole body irradiation with 6.5Gy of X-ray. And the mice were sacrificed at nine days after the irradiation. The RS group were significant increase in the weight of spleen (p<0.05) and the numbers of endogenous spleen colony (p<0.001) and WBC (p<0.05) especially neutrophils, lymphocytes and monocytes in comparison with the RC group. The RR group were significant increase in the numbers of endogenous spleen colony (p<0.01) and WBC (p<0.05) especially neutrophils, lymphocytes and eosinophils in comparison with the RC group. The RS group exhibited a more increase in the weight of spleen and the number of endogenous spleen colony than the RR group. The values of ALT (alanine transaminase) and AST (aspartate transaminase) were significantly elevated (p<0.05) by radiation and they were significantly decreased (p<0.05) in the RS group to the values of the NC group. The value of TBARS(thiobarbituric acid reactive substance) was significantly elevated (p<0.01) by radiation and it was significantly decreased (p<0.05) in the RS group. And the values of SOD (superoxide dismutase) and CAT (catalase) were significantly increased (p<0.05) in the RS group in comparison with the RC group. Taken together the above results, sun ginseng demonstrated the protective effects on hepatocytes and immune and hematopoietic cells in mice exposed to medium dose of irradiation, and those radioprotective effects were a little higher or similar to the red ginseng.

• Journal of Nutrition and Health
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• v.36 no.8
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• pp.793-800
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• 2003

This study was conducted to investigate the antioxidative effects of Paeonia lactiflora (PL) seeds on antioxidative defense system and lipid peroxidation of liver in rats fed high-cholesterol diet. Sprague-Dawley male rats weighing 100 $\pm$10g were randomly assigned into five experimental groups fed 0.5% cholesterol ; HC group which was not supplemented PL seeds extract, 0.1% methanol extract diet group (MP1 group), 0.2% methanol extract diet group (MP2 group), 0.05% ether-souble fraction diet group (EP1 group) and 0.1 % ether-souble fraction diet group (EP2 group). Experimental diets were fed ad libitum to the rats for 3 weeks. The activity of hepatic superoxide dismutase (SOD) was not significantly different among all the high cholesterol diet groups. The hepatic glutathione peroxidase (GSHpx) activity in MP2 group was increased to 27% compared to HC group. The activity of hepatic catalase (CAT) was not significantly different among the all high cholesterol diet groups. The hepatic glutathione S-transferase (GST) activity in the EP1 and EP2 groups were increased to 12% and 13%, respectively, as compared to HC group. The levels of hepatic TBARS in the MP1, MP2, EP1 and EP2 groups were reduced by 18%, 21%, 20% and 23%, respectively, as compared with HC group. The contents of lipofuscin in liver was not significantly different among all the experimental groups. The results indicated that PL seeds extract may be reduced oxidative damage by activating antioxidative defense system of hepatic in rats fed high-cholesterol diets. (Korean J Nutrition 36(8): 793∼800, 2003)