• Archives of Pharmacal Research
• /
• v.20 no.5
• /
• pp.404-409
• /
• 1997

Trout CYP1A-CAT expression construct was generated by cloning -3.5 Kb $5^I$ flanking DNA of trout liver CYP1A gene in front of CAT gene at pCAT-basic vector. Hepa 1 cells, which are known to contain a functional arylhydrbcarbon $receptor^I$ were transfected with trout CYP1A-CAT using lipofectin. 3-Methylcholanthrene (1 nM) was added into hepa 1 cells in culture in order to examine if $5^I$ flanking DNA of trout CYP1A gene could interact with mouse transactivating factors to bring about transcription of the chloramphenicol acetyltransferase(CAT) reporter gene. The level of CAT protein was measured by CAT ELISA and the level of CAT mRNA was determined by RTPCR. The treatment of 1 nM 3-methylcholanthrene resulted in two fold increases in CAT protein as well as CAT mRNA compared to untreated control hepa 1 cells. These data indicate that arylhydrocarbon receptors of mouse hepa 1 cells are functional to activate exogenously transfected trout CYP1A-CAT construct in terms of both transcription and translation of CAT. We also examined the effect of 3-methylcholanthrene on endogenous cyplal activity in hepa 1 cell. 3-Methylcholanthrene (1 nM) treatment to hepa 1 cells trahsfected with trout CYP1A-CAT construct stimulated the level of cyp1a1 mRNA by two folds and the activity of ethoxyresorufin-O-deethylase by two fold compared to that of control cells. In this study we reported that trout CYP1A-CAT reporter gene expression construct could be expressed by 3-methylcholanthrene treatment in mouse hepa 1 cells. Thus trout CYP1A-CAT could serve as a good model to study the mechanism of regulation of CYP1A1 gene expression.

• Journal of Microbiology and Biotechnology
• /
• v.31 no.2
• /
• pp.259-271
• /
• 2021

Many bacteria metabolize aromatic compounds via catechol as a catabolic intermediate, and possess multiple genes or clusters encoding catechol-cleavage enzymes. The presence of multiple isozyme-encoding genes is a widespread phenomenon that seems to give the carrying strains a selective advantage in the natural environment over those with only a single copy. In the naphthalene-degrading strain Pseudomonas putida ND6, catechol can be converted into intermediates of the tricarboxylic acid cycle via either the ortho- or meta-cleavage pathways. In this study, we demonstrated that the catechol ortho-cleavage pathway genes (catBICIAI and catBIICIIAII) on the chromosome play an important role. The catI and catII operons are co-transcribed, whereas catAI and catAII are under independent transcriptional regulation. We examined the binding of regulatory proteins to promoters. In the presence of cis-cis-muconate, a well-studied inducer of the cat gene cluster, CatRI and CatRII occupy an additional downstream site, designated as the activation binding site. Notably, CatRI binds to both the catI and catII promoters with high affinity, while CatRII binds weakly. This is likely caused by a T to G mutation in the G/T-N11-A motif. Specifically, we found that CatRI and CatRII regulate catBICIAI and catBIICIIAII in a cooperative manner, which provides new insights into naphthalene degradation.

• Journal of the Semiconductor & Display Technology
• /
• v.20 no.2
• /
• pp.131-136
• /
• 2021

EtherCAT is an Ethernet-based fieldbus system standardized in IEC 61158 and SEMI, and widely used in the fields of factory automation, semiconductor equipment and robotics. In this paper, a real-time EtherCAT master is implemented on Linux operating systems and its performances are evaluated. To enhance the real-time capability of mainline Linux kernel, Xenomai is applied as a real-time framework and an open source EtherCAT master stack, Simple Open EtherCAT Master (SOEM), is installed on it. Unlike other studies, the real-time performance of the EtherCAT master is evaluated at the output of the network interface card, so that the evaluation results include all possible effects from the EtherCAT master system. The implemented EtherCAT master can send and receive packets up to 20KHz control frequency with low jitter, even in stressed condition.

• Journal of Korean Academy of Fundamentals of Nursing
• /
• v.13 no.3
• /
• pp.457-466
• /
• 2006

Purpose: This study was done to investigate oncology nurses' perception, attitude, knowledge and practice of CAT and to describe nurses' needs for education on CAT Method: A cross-sectional descriptive study was conducted. A total of 270 oncology nurses working at four medical centers in Seoul and Kyungki participated in this study. They completed the questionnaire between December 2005 and February 2006. Results: Oncology nurses perceived CAT as possible nursing interventions, but felt that there was no conclusive evidence on effectiveness/safety. Of the participants 44.4% have used CAT for themselves, and 73.9% of them were satisfied after using CAT. Two biggest reasons for CAT use were symptom control and emotional support. However, 80.8% of the participants had not had any training related to CAT Both nurses who have used CAT and those who have been trained to practice CAT represented a more positive perception toward CAT (p=.000). The biggest perceived obstacles to the practice of CAT were concerns about side effects (71.2%) and lack of knowledge and skill (69.6%). The most favorable CAT modalities in both practice and training were mind-body medicine. Conclusion: Overall oncology nurses' perception toward CAT was encouraging. However a high number of the nurses reported lack of knowledge and skill in CAT Education and training strategy for CAT need to be developed.

• Korean Journal of Microbiology
• /
• v.27 no.3
• /
• pp.194-200
• /
• 1989

The nucleotide sequence of inducible chloramphenicol acetyl-transferase(CAT) gene isolated from a small plasmid pSBK203 of Staphylococcus aureus was determined. The base sequence shows that structural gene of pSBK203-CAT encodes a protein of 213 amino acids and has a leader region which encodes a short polypeptide of 9 amino-acids in its upstream. vertical bar /sup 35/S vertical bar-Methionine labelled CAT gene product in minicell showed almost same mobility with pC194-CAT of which molecular weight is 24Kdal on polyacrylamide gel electrophoresis. Predicted amino acid sequence of pSBK203-CAT has revealed a high degree of homology with the CATs of pC194 and pC221 than those of cat-86, Tn9 and proteus mirabilis PM13.

• Journal of Korean Library and Information Science Society
• /
• v.46 no.4
• /
• pp.451-470
• /
• 2015

The purpose of this study is to review the processing of Korean related personal identities included in the OCLC WorldCat Identities Network and propose how to improve its errors. With twelve samples of English and Korean personal and subject identities, their related identities and works were examined, and their bibliographic records were retrieved from WorldCat. In searching WorldCat, inconsistencies in default search queries and the processing of identities with a date or a fuller form of name were observed.

• IEMEK Journal of Embedded Systems and Applications
• /
• v.9 no.2
• /
• pp.117-125
• /
• 2014

The EtherCAT protocol has become a very promising alternative for real-time industrial application due to its ability to eliminate uncertainties in the Ethernet. However, the extended markup language (XML) for the EtherCAT network system, which is required in the design, lacks systematic development to take advantage of model transformation techniques. This paper focuses on the system development procedure of the EtherCAT-based encoder system using the CANopen over EtherCAT (CoE) protocol. UML modeling is being adapted to design for XML of EtherCAT-based encoder system. To this purpose, this paper analyzes the object dictionary (OD) of a commercial encoder and CANopen over EtherCAT. A UML diagram is then designed based on the analysis, and XML is generated through the designed UML diagram. Finally, an experimental test_bed for the EtherCAT-based encoder system is implemented and its performance is compared with a commercial encoder.

• Journal of East-West Nursing Research
• /
• v.21 no.2
• /
• pp.110-118
• /
• 2015

Purpose: This study aimed to provide a basic resource for developing nursing interventions and educational programs for complementary and alternative therapies (CAT) by examining nursing students' perception, experience, and attitudes toward CAT. Methods: Data were collected targeting 230 nursing students from October 20 to November 10, 2014, and analyzed by using descriptive statistics, t-test, ${\chi}^2$-test, and ANOVA with SPSS/WIN 18.0. Results: Results showed that CAT were performed by CAT specialists in combination with hospital treatments for individuals suffering from chronic pain and musculoskeletal diseases. Nursing students obtained information on and experience of CAT through the Internet. CAT were used for psychological and mental stability as a supplementary treatment. The majority of the participants used Yoga followed by massage as a sub-therapy of the program. They said that openness of medical staff and active communication are important for having a positive attitudes toward CAT. There are differences in attitudes toward CAT according to gender, student levels, and previous experience on CAT. Conclusion: It is crucial for nursing students to have right perception, experience, and attitudes toward CAT in order to apply CAT to the clinical field.

• Journal of Ecology and Environment
• /
• v.33 no.1
• /
• pp.3-13
• /
• 2010

The Tsushima leopard cat, Prionailurus bengalensis euptilurus, a small felid, inhabits only the Tsushima Islands in Japan. Previous studies of the Tsushima leopard cat revealed that natural factors; including sex, reproductive activity, season, and prey distribution and abundance affect leopard cat home range variation and habitat use. In this study, we focused on clarifying how anthropogenic factors influenced home range variation and habitat use of a male Tsushima leopard cat living near a suburban area in January, March, May and September 2005 using radio-tracking. The home range size (100% MCP) of this cat was $0.78\;{\pm}\;0.26\;km^2$ (mean ${\pm}$ SD, n = 4 tracking sessions) across the whole study period. However, the cat did not use all parts of its home range uniformly; rather it used some habitat types selectively. The cat avoided agriculture areas and residential areas in all of the tracking-sessions. On the other hand, the cat showed a weak preference for artificial structures and a strong preference for baiting sites in January and March, while it avoided them in May, and no baiting site was included in its home range in September. These results suggest that anthropogenic factors influenced the ranging patterns and habitat use of the leopard cat living near a suburban area. Artificial structures might provided good resting spaces for the cat in bad weather. When the density of its main prey was low in the winter, the cat tended to rely on artificial prey and had a small home range size.

• Mycobiology
• /
• v.41 no.3
• /
• pp.145-148
• /
• 2013

Vegetative growth signaling of the opportunistic human pathogenic fungus Aspergillus fumigatus is mediated by GpaA ($G{\alpha}$). FlbA is a regulator of G protein signaling, which attenuates GpaA-mediated growth signaling in this fungus. The flbA deletion (${\Delta}flbA$) and the constitutively active GpaA ($GpaA^{Q204L}$) mutants exhibit enhanced proliferation, precocious autolysis, and reduced asexual sporulation. In this study, we demonstrate that both mutants also show enhanced tolerance against $H_2O_2$ and their radial growth was approximately 1.6 fold higher than that of wild type (WT) in medium with 10 mM $H_2O_2$. We performed quantitative PCR (qRT-PCR) for examination of mRNA levels of three catalase encoding genes (catA, cat1, and cat2) in WT and the two mutants. According to the results, while levels of spore-specific catA mRNA were comparable among the three strains, cat1 and cat2 mRNA levels were significantly higher in the two mutants than in WT. In particular, the ${\Delta}flbA$ mutant showed significantly enhanced and prolonged expression of cat1 and precocious expression of cat2. In accordance with this result, activity of the Cat1 protein in the ${\Delta}flbA$ mutant was higher than that of $gpaA^{Q204L}$ and WT strains. For activity of the Cat2 protein, both mutants began to show enhanced activity at 48 and 72 hr of growth compared to WT. These results lead to the conclusion that GpaA activates expression and activity of cat1 and cat2, whereas FlbA plays an antagonistic role in control of catalases, leading to balanced responses to neutralizing the toxicity of reactive oxygen species.