• Title/Summary/Keyword: carotenoid pigment

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Studies on the Natural Pigments (Part I) -Contents and Properties of Gardenia Jasminoides Ellis Pigment- (천연색소(天然色素)에 관(關)한 연구(硏究)제1보(第一報) -치자색소(梔子色索)의 함량(含量) 및 성질(性質)에 관(關)한 연구(硏究)-)

  • Kim, Dong-Yun;Kim, Kwan
    • Applied Biological Chemistry
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    • v.18 no.2
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    • pp.98-101
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    • 1975
  • The components of Gardenia pigment in rind, flesh, fresh fruit and stored fruit, its heat resistance and stability in pH, its dilution and colour-degree were studied. The results are summarized as follows; 1. The components of CROCIN in fresh fruit and stored fruit were 0.0157% and 0.0136% respectively. One year storage of stored fruit resulted in the decrease of 15% of CROCIN. 2. The components of Gardenia pigment in flesh were contained nearly twice as much as in rind. 3. There were traces of ${\beta}-carotene$ and some unknown carotenoid pigments. 4. Gardenia pigment was stable at $100^{\circ}C$ for 60 minutes, but, at $150^{\circ}C$ for 30 minutes, nearly half of them was destructed. 5. Gardenia pigment turned to colurless but was stable in neutral or alkali condition after 10 days. 6. Supercially, dark Gardenia pigment looked yellowish red and thin Gardenia pigment looked yellow: however, each ${\lambda}_{max}$. was all the same.

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Utilization of Ascidian(Halocynthia roretzi) Tunic -2. Optimum Level of Carotenoid Extracts from Ascidian Tunic for the Pigmentation of Rainbow Trout, Oncorhynchus mykiss- (우렁쉥이 껍질성분의 이용에 관한 연구 -2. 무지개송어 육색 개선을 위한 우렁쉥이 껍질 추출물의 최적 첨가량-)

  • LEE Kang-Ho;KANG Seok-Joong;CHOI Byeong-Dae;CHOI Young-Joon;YOUM Mal-Gu
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.27 no.3
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    • pp.240-246
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    • 1994
  • This study was designed to determine the optimum pigment concentration supplemented in diet and feeding periods on the pigmentation of rainbow trout(Oncorhynchus mykiss) by using acetone-extracts of ascidian tunic as a natural pigment source. The eight pigmented diets contained carotenoid of ascidian tunic extracts at concentrations of 0, 200, 400, 800, 1,600 and 3,200mg/kg of diet, carophyll pink at concentration of 800mg/kg and commercial diet. No difference in pigment concentration was found between the ascidian extracts group and the control group until 4 weeks, but the redness of muscle and integument in the 1,600, 3,200mg/kg diet and carophyll pink was increased in the dorsal and caudal areas of fish from 6 weeks of age. In the sensory panel test, fish fed the ascidian tunic extracts diet were similar to those fed the carophyll pink diet. The optimum concentration and feeding periods for pigmentation of rainbow trout was found to be ascidian tunic extracts of 1,600mg/kg diet for 8 weeks.

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Needle Life Span, Photosynthetic Pigment and Nitrogen Allocation of Picea jezoensis in Korea (우리나라 가문비나무의 침엽 수명, 광색소 및 질소 배분 특성)

  • Han, Sim-Hee;Kim, Du-Hyun;Kim, Gil Nam;Yun, Chung-Weon
    • Journal of Korean Society of Forest Science
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    • v.101 no.1
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    • pp.62-68
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    • 2012
  • We have investigated needle life span, photosynthetic pigment and nitrogen allocation pattern in the needle of Picea jezoensis in the three populations (Gyebangsan, Deogyusan and Jirisan) to find out growth environmental condition which needs the strategy development of conservation and restoration against population decline. Needle survival rate was the highest in Gyebangsan (87.0%) and the lowest in Jirisan (71.6%), and it decreased with the increase of needle age. Needle chlorophyll a and b in Deogyusan and Jirisan showed higher content in older needle, but chlorophyll content in Gyebangsan was the highest in 2-year-old needle. Carotenoid content was the highest in the needle in Gyebangsan, and it increased along with needle age. Chlorophyll a/b ratio showed the highest value in Jirisan and the lowest value in Gyebangsan. Chlorophyll/carotenoid ratio decreased with needle age, Needle nitrogen content was the highest in Deogyusan (1.51%) and the lowest in Gyebangsan (1.40%), and the older needle had the lower content. In contrast, the highest chlorophyll/nitrogen ratio represented the oldest needle.

Analysis of Pigments and Thylakoid Membrane Proteins in Photosystem I - Mutants from Synechocystis sp. PCC6803 (Synechocystis sp. PCC6803을 이용한 Photosystem I- mutants의 색소 및 틸라코이드막 단백질 분석)

  • 전은경;장남기
    • Asian Journal of Turfgrass Science
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    • v.11 no.1
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    • pp.45-58
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    • 1997
  • Pigments and thylakoid membrane proteins were investigated in wild type and PS I- mutants from Synechocystis sp. PCC6803 Comparing morphological features, B2 was less fluorescent than the other strains. The contents of chlorophyll a were propotional to the FNR activity in thylakoid membrane. The FNR activity of mutants was lower than that of wild type. In the result of pigments analysis, mutants had smaller cholophyll a than that of wild type. The major carotenoid was found to he $\beta$-caroene, but aeaxanthin was barely detected in thylakoid membrane of mutants. The polypeptide, 14.8kD was detected by electrophoresis in mutants. It was considered to be the modification of 15.4kD in wild type. Membrane polypeptides of 17.6 and 19.7kD were not detected in mutants. In the result of western blotting, subunit I was detected in all strains, but subunit II was barely detected in mutants. Subunit II was not detected in B2 at all. In view of the results so far achieved, the changes of contents of chlorophyll and zeaxanthin were affected by the defficiency or modification of functional domain in subunit I. Also the modification in subunit I affected the subunit II- binding site in PS I. As the result, efficiency of photosynthesis was decreased. Key words: Synechoystis sp. PCC6803, PS I - mutant, Photosynthetic efficiency, Pigment,Thylakoid membrane proteins, Subunit I, II.

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Content Changes of Pigments and Antioxidants of Dried Samnamul (Aruncus dioicus) and Daraesoon (Actinidia arguta) during Rehydration and High Temperature Cooking (건조 삼나물과 다래순의 재수화와 고온 가열조리 중 색소와 산화방지성분의 함량 변화)

  • An, Haechun;Choe, Eunok
    • Korean journal of food and cookery science
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    • v.32 no.4
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    • pp.383-389
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    • 2016
  • Purpose: This study was conducted to evaluate the effect of rehydration and subsequent heating at high temperature on the pigments and antioxidants of dried samnamul (Aruncus dioicus) and daraesoon (Actinidia arguta). Methods: Rehydration included 16 h-soaking in cold water, and 30 min-boiling and 1 h-infusion in water. Rehydrated samnamul and daraesoon were heated at $180^{\circ}C$ for 10 or 20 min with or without perilla oil addition (10%) for cooking. Pigments and antioxidants were determined by HPLC and spectrophotometry. Results: Rehydration caused decreases in pigment and polyphenol contents, but increase in tocopherol content. Cooking by heating without addition of perilla oil resulted in increases in chlorophyll and carotenoid contents, but decreases in polyphenol and tocopherol contents. Decrease in tocopherol content by heating at $180^{\circ}C$ was reversed by the addition of perilla oil. Conclusion: This study strongly suggested that cooking of samnamul and daraesoon at $180^{\circ}C$ with perilla oil could improve color, texture, and potential health functionality by recovering the loss of antioxidants and pigments with antioxidant activity.

Studies on the Growth of Freshwater Algae by Biocides I. On the Growth of Chlamydomonas reinhardii (Biocide에 의한 담수조류의 생장에 관한 연구 I. Chlamydomonas reinhardii의 생장에 미치는 영향)

  • 이은경
    • Journal of Plant Biology
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    • v.25 no.3
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    • pp.113-121
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    • 1982
  • The effects of 6 biocides on the growth of Chlamydomonas reinhardii in pure culture were studied. For the batch culture assay of biocides, the growth rate in control tubes should be compared with in the test tubes and the effective concentration calculated on the basis of the percent decrease in growth rate at the different toxicant concentration. The concentrations at which 50% decrease in growth rate was observed are $40{\mu}g$/l for butachlor and $350{\mu}g$/l for alachlor in herbicide, $900{\mu}g$/l for phenazine-5-oxide and $3,400{\mu}g$/l for isoprothiolane in fungicide, and $3,330{\mu}g$/l for fenthion and $332,500{\mu}g$/l for trichlorfon in insecticide. The inhibitory effect on the growth of Chlamydomonas reinhardii by the treatment of various biocide concentrations was decreased in order of herbicide>fungicide>insecticide. Chlorophyll and carotenoid content per cell were increased, whereas chlorophyll a/b ratio was hardly affected by biocides. The effects of biocides on pigment content were also decreased in order of herbicide>fungicide>insecticide, which suggested the relationship between the effect of biocides on the pigment content and inhibition of growth rate.

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Growth regulation of cow1 rice mutant seedlings by blue light

  • Goh, Chang-Hyo;Ko, Suk-Min;Park, Hee-Yeon;Kim, Yeon-Ki;Kim, Yong-Woo;Kim, Young-Joo;Sun, Hyeon-Jin;Moon, Yong-Hwan;Lee, Hyo-Yeon
    • Journal of Plant Biotechnology
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    • v.37 no.4
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    • pp.465-471
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    • 2010
  • We assessed whether the cow1 mutant defects are associated with growth of Tos17 and T-DNA insertional rice in blue light (BL). Growth of oscow1 mutants which encoded a member of the YUCCA protein family was retarded in BL. Root to shoot ratios of the mutants were reduced about 2 times lower in the absence of NAA and about 2.5 times lower in the presence of NAA; the shoot growth was not significantly changed by NAA addition. Photosynthetic activity of the mutants was however inhibited in high light. Pigment analysis showed significant difference between wild-type (Chl a:b = 3.02) and mutants (3.84). Carotenoid contents of the mutants were also decreased considerably, implying the involvement of cow1 in pigment formation. These findings lead us to suggest that the growth retardation of oscow1 mutant plants by BL results from the difference of photosynthetic activity in part.

Volatile Components of Phellinus linteus from Different Areas (산지가 다른 상황버섯의 휘발성성분)

  • Jang, Eun-Young;Im, Sung-Im;Jeong, Yong-Kee;Choi, Sung-Hee
    • Korean Journal of Food Science and Technology
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    • v.38 no.2
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    • pp.159-164
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    • 2006
  • Volatile components of Phellinus linteus produced from different areas were collected by simultaneous steam distillation-solvent extraction method (SDE). Concentrated extracts analyzed and identified by GC and GC-MS showed musty and earthy characteristics. 2-Methylphenol, methoxy benzene, coumaran, azulene, ${\alpha}-cedrene,\;{\alpha}-longipinene,\;{\beta}-selinene,\;{\alpha}-selinene$, camphor, ${\gamma}-ionone,\;{\beta}-ionone$, phytol, and borneol not reported in other edible mushrooms, were identified and/or tentatively identified in P. linteus for the first time. Main volatile components of P. linteus (Busan-Jinsung: BJ) were phytol from chlorophyll and methoxy benzenes having musty odor. Volatile components of P. linteus (Jinju-Kumwhang: JK) resembled those of BJ, but with high concentration of phenylacetaldehyde contributing to flower-odor. P. linteus (Cheju-Gullim: CG) contained low concentration of methoxy benzenes, but high concentration of phenylacetaldehyde. Low concentrations of ${\gamma}-ionone\;and\;{\beta}-ionone$ were identified in three kinds of P. linteus. They appeared to have been produced from degradation of carotenoid, which suggests P. linteus contains a carotenoid pigment.

Pigment Stability of Lavers Porhyra tenera Kjellman during Processing and Storage (김의 가공 저장중에 있어서의 색소의 안정도)

  • LEE Kang-Ho
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.2 no.2
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    • pp.105-133
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    • 1969
  • The stability of three major pigments in lavers, namely chlorophylls, carotenoids, and phy-cobilins, during processing and storage was studied. The results of the pigment retention in fresh lavers during storage at low temperatures, of heat lability during heat treatment, and of the pigment loss during storage are summarized as follows: 1. The contents of chlorophyll a, xanthophyll (lutein+zeaxanthin), and carotene ($\alpha-+\beta-carotene$) averaged 1,525, 627, and $409{\mu}g/g$ of dry samples respectively The comparative composition of four carotenoids, lutein, $\beta-carotene$, zeaxanthin, and $\alpha-carotene$, was 35.9, 33.7, 12.2, and $5.8\%$ respectively. 2. In storage of fresh lavers at room ternperature ($20-25^{\circ}C$), 2 to $5^{\circ}C$, and $-15^{\circ}C$, the pigments generally were retained more at 2 to $5^{\circ}C$ than others while carotene only showed more retention at $-15^{\circ}C$ than at 2 to $5^{\circ}C$. Phycocyanin tended to decrease more rapidly than phyco-erythrin at low temperatures. 3. In regard to heat treatment of both dried and wet materials at 40, 60, 80, $100^{\circ}C$ respectively, the pigments were more stable at lower temperatures than at higher temperatures in both cases of materials. Carotenoids were retained more in wet materials than in the sun dried. Xanthophylls showed higher heat stability than carotenes. Phycoerythrin was considered more heat labile than phycocyanin, particularly at higher temperatures. 4. In storage of heat treated lavers for 50 days at room temperature, the retention of pigments appeared differently in accordance with the temperatures of treatment The materials treated at $60^{\circ}C$ showed better relention of pigments than those treated at the other temperatures. Degradation rate of xanthopllylls, carotenes, and phycobilins was decreased in the materials treated at higher temperatures, showing a tendency of great pigment retention even for a long period storage. Chlorophyll il was retained more in sun dried materials whereas xanthophyll and carotene were retained more in the wet. 5. In order to maintain a higher pigment stability during storage, the materials must be treated at $60^{\circ}C$ in a dryer with rapid ventilation until the moisture level reaches about $6.0\%$. In case of treatment at a higher temperature than $60^{\circ}C$, they must be treated for a short period of time to minimize pigment degradation by heat. 6. The lavers treated with the solutions of pH around 7.8 showed a higher retention of chlorophyll a. When treated with the solutions of algal extracts such as agar and alginic acid, uniform layer of laver sheets and better surface gloss of dried layers were obtained.

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Molecular Cloning and Characterization of the Gene Encoding Phytoene Desaturase from Kocuria gwangalliensis (Kocuria gwangalliensis 유래 phytoene desaturase 유전자의 cloning과 특성 연구)

  • Seo, Yong Bae;Choi, Seong Seok;Nam, Soo-Wan;Kim, Gun-Do
    • Microbiology and Biotechnology Letters
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    • v.45 no.3
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    • pp.226-235
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    • 2017
  • Carotenoids such as phytoene, lycopene, and ${\beta}-carotene$ are used as food colorants, animal feed supplements, and for human nutrition and cosmetic purposes. Previously, we reported the isolation of a novel marine bacterium, Kocuria gwangalliensis, which produces a pink-orange pigment. Phytoene desaturase (CrtI), encoded by the gene crtI, catalyzes lycopene formation from phytoene and is an essential enzyme in the early steps of carotenoid biosynthesis. CrtI is one of the key enzymes regulating carotenoid biosynthesis and has been implicated as a rate-limiting enzyme of the pathway in various carotenoid synthesizing organisms. Here, we report the cloning of the crtI gene responsible for lycopene biosynthesis from K. gwangalliensis. The gene consisted of 1,584 bases encoding 527 amino acid residues. The nucleotide sequence of the crtI gene was compared with that of other species, including Kocuria rhizophila and Myxococcus xanthus, and was found to be well conserved during evolution. An expression plasmid containing the crtI gene was constructed (pCcrt1), and Escherichia coli cells were transformed with this plasmid to produce a recombinant protein of approximately 57 kDa, corresponding to the molecular weight of phytoene desaturase. Lycopene biosynthesis was confirmed when the plasmid pCcrtI was co-transformed into E. coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis. The results from this study will provide valuable information on the primary structure of K. gwangalliensis CrtI at the molecular level.