• 미생물학회지
• /
• 제24권1호
• /
• pp.38-45
• /
• 1986

Rhizopus oryzae 균사체 내의 인지질 구성 성분은 6종으로 나타났고 sodium deoxycholate와 sodium dodecyl sulfate를 각각 0.48mM, O.18mM의 낮은 농도로 영양배지에 첨가했을때 성장군략의 형태변화와 아울러 미량 존재하던 cerdiolipin과 phospha tidylinositol 그러고 Iy sophospholipid의 함량이 증가하였다. 또한 phospholipase는 A와 C, 그리고 D가 존재하였으며 A와 D는 sodium deoxycholate에 의하여. C는 sodium dodecyl sulfate에 의하여 활성도 증가를 나타냈다.

• Journal of Microbiology and Biotechnology
• /
• 제29권10호
• /
• pp.1591-1602
• /
• 2019

To shed light on the genetic basis of salt tolerance in Enterococcus faecium, Enterococcus faecalis, and Tetragenococcus halophilus, we performed comparative genome analysis of 10 E. faecalis, 11 E. faecium, and three T. halophilus strains. Factors involved in salt tolerance that could be used to distinguish the species were identified. Overall, T. halophilus contained a greater number of potassium transport and osmoprotectant synthesis genes compared with the other two species. In particular, our findings suggested that T. halophilus may be the only one among the three species capable of synthesizing glycine betaine from choline, cardiolipin from glycerol and proline from citrate. These molecules are well-known osmoprotectants; thus, we propose that these genes confer the salt tolerance of T. halophilus.

• 한국식품과학회지
• /
• 제19권1호
• /
• pp.23-28
• /
• 1987

Silicic acid chromatography로 순수정제한 대두유 의 향미안정성에 미치는 인지방질의 영향을 조사하였다. 1ppm ferrous iron을 첨가한 순수정제 대두유에 300ppm 의 phosphatidyl choline(PC), phosphatidyl ethanolamine(PE), phosphatidyl inositol(Pl), phosphatidic acid(PA), phosphatidyl glycerol(PC), cardiolipin(CL)을 개별적으로 첨가하여 $60^{\circ}C$의 오븐에서 10일간 저장한후, 시료병 윗 공간내의 총휘발성물질양 및 산소의 잔유량으로써 향미안정성을 측정하였다. 1ppm의 철분을 함유한 시료의 향미안정성은 인지방질을 첨가함으로써 개선되었는데, 이는 인지방질의 금속이온에 대한 chelating효과에 기인한 것으로써, PA. PE, PC, PG, CL, Pl의 순으로 효과적이었다. 그러나 철분을 함유하지 않은 순수정제 대두유에 인지방질을 첨가했을 경우, 그 향미안정성은 감소하였다. 즉 금속 이온이 없을 경우 인지방질은 산화촉진효과를 나타내었는데, 이는 인지방질의 극성부분으로 인하여, 유지의 계면에 용존산소의 농도가 증가하는 데에서 기인하는 것으로 생각한다. 그러므로, 유지의 향미안정성을 개선시키기 위하여는 유지내 인지방질의 농도를 금속이온을 chelating시킬 적당량만을 유지하는 것이 바람직한 것으로 생각된다.

• Journal of Preventive Medicine and Public Health
• /
• 제7권2호
• /
• pp.293-298
• /
• 1974

This study was carried out to contribute to the control of Venereal diseases amnog prostitutes living aroung US army camps in Daegu area. The status of those who registrated in our Bongdeok Venereal Diseases Clinic was observed during a year from October 1, 1973 to September 31, 1974. Most of them were playmates of foreign soldiers. Forty eight percent of the total 241 were 20 to 24 years in age. About 70 percent were under elementary school level in educational background. The reason for prostitution was hard living and economic problems in about 94 percent of the cases studied. The cases studied within one year from the present time were 104, 43.3 percent. Although the full number of monthly vistits in eight or nine (Two times a week), the monthly mean was found to be 5.5. The result of cardiolipin test was 4.6 percent positive and 9.3 percent, false positive. Gram negative diplococci was found to be 4.3 percent, pus cell, 49 percent and staphylocci 18.1 percent. The patients were chiefly cured by ampicillin and kanamycin, although procain penicillin was also used. More control mathods must be introduced and promoted along with tracing methods and cultures for gonorrhea.

• Molecular & Cellular Toxicology
• /
• 제5권3호
• /
• pp.207-215
• /
• 2009

Despite wide therapeutic use of CpG ODN against infection, allergy and cancer, the safety and toxicity of CpG ODNs were poorly delineated. Thus, we investigated whether optimal dosing of CpG ODN would affect immunotoxicological parameters in NZB/NZW F1 mice. Comparisons were made among control, non-CpG ODN and mouse CpG ODN ($10{\mu}g$)-treated groups for 4 weeks. To gauge the immunotoxicity of CpG ODNs, we measured nonspecific parameters, degree of lupus nephritis, proteinuria, or autoantibody, and cytokine expression in mRNA level of lymphocytes. We found that there were no significant differences among groups in nonspecific immunotoxicological profiles and in evaluation profiles of glomerulonephritis. However, titer of anti-dsDNA and anti-cardiolipin antibodies in mouse CpG ODN group rose three or eight-fold higher than in control group. Collectively, CpG ODN might be clinically less immunotoxic in terms of clinical profiles in lupus-prone NZB/NZW F1 mice, in spite of high autoantibody titer in CpG ODN treated groups.

• 한국식품영양과학회지
• /
• 제20권5호
• /
• pp.473-478
• /
• 1991

After the glycolipid(GL) and phospolipid (PL) of flint corn during a growth process were fractionated by silicic acid column chromatography (SACC), the GL and PL of polar lipids were separated by thin layer chromatography (TLC)and quantitative by TLC scanner and the fatty acid composition in polar lipids were determined by gas chromatography (GC). Of the GL in polar lipids were separated by TLC, monoglycosyl diacylglycerol (8.3~29.2%) was the major component, and monoglycosyl ceramide, monoglycosyl sterol were also found as minor components. Of the PL, phosphatidyl choline (27.2~29.5%) and phosphatidyl inositol (42.9~79.1%) were the major components, and phosphatidyl ethanolamine and cardiolipin were also persont in the PL. The major fatty acids in the GL were oleic(27.1~37.1%), linoleic (13.2~35.7%) and palmitic (22.5~25.6%) acids. The major fatty acids in the PL were palmitic (46.5~52.3%), heptadecanoic (23.0~25.1%) and oleic(7.2~14.6%) and GL contained a higher percentage of unsa-turated fatty acids, but PL presented that of the saturated fatty acids.

• 한국환경보건학회지
• /
• 제23권2호
• /
• pp.1-11
• /
• 1997

Copper chloride 10 ppm, manganese chloride 100 ppm, nickel chloride 50 ppm을 각각 처리한 Bacillus subtilis를 배양하는 동안에 이들 세포에서 일어나는 인지질 생합성 및 지방산 조성의 변화를 대조구와 비교하여 분석하였다. 세포의 생장과 phosphatidylethanloamine, phosphatidylcholine, phosphatidylglycerol, cardilolpin은 대조구에 비해 금속 화합물 처리구에서 저해되었는데 copper chloride가 가장 큰 억제 효과를 나타내었다. 그러나 phosphatidylinositol은 금속화합물의 영향을 받지 않았다. 인지질 생합성에 이용된 주요 지방산은 대조구에서는 palmitic acid(평균 19.00%)와 stearic acid(평균 9.58%)로 나타났다. 그러나 copper chloride 처리구는 palmitic acid(평균 17.38%)와 oleic acid(평균 15.99%)가 인지질 생합성에 이용된 주요 지방산이었고, manganese chloride 처리구는 palmitic acid(평균 15.00%)와 myristic acid(평균 14.24%), nickel chloride 처리구는 oleic acid(평균 17.87%)와 stearic acid(평균 13.78%)가 인지질 형성에 이용되었다.

• Childhood Kidney Diseases
• /
• 제5권2호
• /
• pp.219-224
• /
• 2001

저자들은 전신성 홍반성 낭창의 특이적인 증상 없이 학교 신체검사중 우연히 발견된 현미경적 혈뇨를 주소로 내원하여 시행한 혈액응고검사(prothrombin time, partial thromboplastin time)와 루푸스 항응고인자 (lupus anticoagulant, LA), cardiolipin에 대한 항체(anticardiolipin antibody, aCL) 검사에서 항인지질증후군으로 진단된 10세 여자 환아에서 신조직 검사를 시행하여 확진한 낭창성 신염 1례를 경험하였기에 문헌고찰과 함께 보고하는 바이다.

• 대한한의정보학회지
• /
• 제11권1호
• /
• pp.52-57
• /
• 2005

Objectives : Recurrent pregnancy loss occurs in approximately 0.5-3% of women. There are many studies concerning immunological factor recently. Therefore, aim of this study is to examine the latest trend of researches concerning recurrent pregnancy loss, and controlled experiment on animals about antiphospholipid antibody. Method : We referred Pubmed site by using searching word of 'recurrent pregnancy loss' (Limits : 2000.1-2004.3, animal) Results and conclusions : 1. We searched 29 papers. Immunological factor : 18 ( about antiphospholipid antibody : 10 ), Chromosomal abnormality : 6, The others : 5 2. In five papers about controlled experiment on animals, (1) Materials : 8-12 weeks old mice / 11.5-day old-rat embryos / New Zealand rabbit (2) Inductions : inject intraperitoneally with human IgG containing antiphospholipid antibodies / culture in a solution of 1 ml medium which contained IgG purified from sera of women / inject intradermally with cardiolipin (3) Treatments : inject intraperitoneally with complement component before / culture in a solution of 1 ml medium which contained IgG purified from sera of women with SLE and RPL or from healthy women / inject intradermally with TFX, 0.9% NaCl (4) Measurements : weight fetuses and placentas, calculate frequency of fetal resorption / after culture, examine the embryos / examine platelet counts, APTT and numbers of live and dead newborns, resorbed fetuses, body mass, newborn viability and survival rates.

• 한국산업보건학회지
• /
• 제4권1호
• /
• pp.43-70
• /
• 1994

The effects of potassium chromate (500ppm/500ppm), potassium dichromate (500ppm/500ppm), cobalt chloride (100ppm/10ppm), methylmercuric chloride (100ppm/10ppm) on the biosynthesis of phospholipid and their composition of fatty acids in E.coli and B.subtilis were analyzed. The contents of phosphatidylethanolamine, phosphatidylcholine, phosphatidylinositol, phosphatidylglycerol, cardiolipin and total lipids in treatment with metal compounds were lower to compare with the control. The major fatty acid utilized for biosynthesis of phospholipid was palmitic acid in control of E.coli and B.subtilis. However, in treatment with metal compounds, changes of fatty acid composition utilized for phospholipid formation were as follows. In E.coli major fatty acids were palimitic acid (ave. 26.26%) and cis-vaccenic acid (ave. 10.94%) in treatment with potassium chromate, palmitic acid (ave. 31.41%/31.42%) and stearic acid (ave. 17.92%/19.41%) in treatment with potassium dichromate and cobalt chloride. And in treatment with raethylmercuric chloride, palmitic acid (ave. 26.66%), stearic acid (ave. 15.50%) and cis-vaccenic acid (ave. 20.59%) were used in phospholipid formation. In B.subtilis, the major fatty acid was palmitoleic acid (ave. 15.29% /10.22%) in treatment with potassium chromate and cobalt chloride, and stearic acid (ave. 16.01%) in treatment with potassium dichromate. On the other hand, cis-vaccenic acid (ave. 9.09%), palmitic acid (ave. 17.23%), stearic acid (ave. 6.66%), myristic acid (ave. 6.34%) and lauric acid (ave. 4.75%) were analyzed into major fatty acids in treatment with methylmercuric chloride. As shown in results, specific fatty acid pattern was came out in treatment with metal compounds according to bacteria and treatments.