• Journal of Plant Biotechnology
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• 제30권4호
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• pp.369-373
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• 2003

In spite of potential benefits of anther culture, low productivity of plant regeneration in some genotypes; e.g. tonsil and indica rice, is one of the major obstacles for practical use of anther culture. This study was conducted to improve cold shock method and carbohydrate source for increasing the efficiency of anther culture in rice. The most common carbon source, sucrose was replaced to maltose, which has two molecules of glucose. Maltose increased callus induction 1.4-to 1.8-fold higher in japonica rice, 3.2-to 11.6-fold in tongil types and 2.7-fold in indica rice IR50. Callus induction was increased from 0.2％ to 12.5％ in maltose medium compared to the medium supplemented with sucrose plus glucose in indica rice "Tetep". A simple procedure of vacuum packaging of panicles during cold shock treatment prolonged not only anther viability more than 15 days but also increased callus induction more than 2-fold compared to open-air storage (conventional method). Combining of above two methods, callus induction was increased 28 to 56％ in japonica, 13 to 33％ in tonsil type and 12 to 31％ in indica rice. Plant regeneration was increased 14 to 35％ in japonica, 10 to 20％ in tonsil and 4 to 15％ in indica rice, respectively.

• 한국식품영양과학회지
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• 제15권3호
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• pp.300-305
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• 1986

식생활개선(食生活改善)과 영양정책(榮養政策)의 기초자료(基礎資料)를 얻고자 1974년부터 1984년(年)까지 에너지원으로써 탄수화물(炭水化物)의 공급형태(供給形態) 및 각공급원(各供給源)의 에너지구성율(構成率)의 변화(變化)를 조사한 결과는 다음과 같다. 1. 식품군별(食品群別) 에너지 공급비율(供給比率)은 1984년 곡류(穀類)가 70%를 차지하고 있다. 곡류(穀類)와 당류(黨類)는 공급(供給) 에너지 비율(比率)이 계속 감소하였으나 당류(糖類), 육류(肉類), 유지류(油脂類) 우유(牛乳) 및 난류(卵類)에 의한 에너지 공급비율(供給比率)이 2배 이상 증가를 보였다. 2. 에너지 공급원은 식물성(植物性) 식품(食品)이 90% 가량 차지하고 있으며 식물성(植物性) 식품(食品) 중(中)에는 전분성(澱粉性) 식품(食品)이 80% 이상을 점유하고 있다. 전분성(澱粉性) 식품(食品) 거의 곡류(穀類)로 전체공급(全體供給) 에너지중에도 1984년에 66% 가량을 차지하고 있다. 3. 각(各) 곡류(穀類)가 공급 에너지 중 차지하는 비율(比率)은 쌀, 밀, 보리의 순이며 쌀의 비율이 감소되고 있어도 아직 전공급(全供給) 에너지의 46%를 차지하고 있다. 보리의 비율(比率)은 급격히 감소하였지만 전분성(澱粉性) 식품(食品)에 의하여 공급(供給)된 에너지 중 곡류(穀類)의 비율(比率)은 거의 일정하였다. 4. 탄수화물(炭水化物)의 공급형태(供給形態)를 보면 전분성(澱粉性) 식품(食品)에 의한 에너지 공급은 서서히 감소하는 반면 정당(精糖)은 높은 증가율을 보였다. 과당(菓糖)과 갈국당(葛菊糖)의 생산량(生産量)도 꾸준히 증가하여 탄수화물(炭水化物)의 섭취가 과당류(菓糖類)로 변화(變化)되고 있었다.

• Asian-Australasian Journal of Animal Sciences
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• 제14권1호
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• pp.48-53
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• 2001

An in vitro study was conducted to examine the effect of sources and the addition levels of carbohydrates on fermentation characteristics, bacterial growth, and hydrogenation of linoleic acid ($C_{18:2}$) by mixed ruminal bacteria. Starch and cellobiose were added to the 200 ml non-selective basal media at the levels of 0.20 and 0.35% (w/v), respectively. Linoleic acid (66.8~79.6 mg) in the absorbed form into the pieces of nylon cloth was also added to the media of 5 treatments including control which was not added with carbohydrate. Three mls of rumen fluid strained through 12 layers of cheese cloth were added to each medium, and were incubated anaerobically in the shaking incubator of $39^{\circ}C$ for 24 hours. During 24 h incubation the pH in incubation media of all treatments was maintained above 6.6 by the addition of sodium bicarbonate. The pH and ammonia concentration of incubation media were not clearly influenced by the sources and addition levels of carbohydrates while additions of carbohydrates increased (p<0.0001) VFA concentration at the 24 h incubation. Molar proportions of acetate were reduced (p<0.0004) while those of propionate were increased (p<0.0006) by the addition of carbohydrates. But the differences in concentration and molar proportions of the VFA were small between the sources or the addition levels. Bacterial growth was faster (p<0.0004) in the starch added treatments than in the cellobiose added ones and control, but no differences were found between addition levels. Increased (p<0.0487) hydrogenation was observed from the starch added treatments compared to the cellobiose added ones, but there was no difference between addition levels.

• 한국물환경학회지
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• 제23권4호
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• pp.482-489
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• 2007

Fluorescence properties and carbohydrate content were investigated using ultrafiltrated size fractions of dissolved organic matters (DOM) originated from different sources. The materials included a treated sewage, an algal organic matter, and a soil leachate, all of which are major constituents of dissolved organic matter in a typical urban river. Four different size fractions were separated from the three sources of each DOM. The size distribution demonstrated that a higher molecular weight fraction was more present in soil leachate compared to two other source DOMs. A higher content of carbohydrates was observed in the following order - algal DOM > treated sewage > soil leachate. A wide range of specific UV absorbance was observed from size fractions of a single source DOM, indicating that aromatic carbon structures are heterogeneously distributed within one source of DOM. The structural heterogeneity was the most pronounced for the soil leachate. The fluorescence index ($F_{450}/F_{500}$) of the treated sewage was similar to that (2.0) typically obtained from autochthonous DOM, suggesting that the treated sewage exhibited autochthonous organic matter-like properties. No protein-like fluorescence intensities were observed for all of the soil leachate size fractions whereas they were observed with two other source DOMs. Based upon the fluorescence peak ratios from fluorescence excitation-emission matrix (EEM), two discrimination indices could be suggested to distinguish three different source DOMs. It is expected that the suggested discrimination indices will be useful to predict the sources of DOM in a typical urban river affected by treated sewage.

• Journal of Nutrition and Health
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• 제30권3호
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• pp.349-359
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• 1997

This study was designed to study sources of variability(subjects, day by day, season, day of week, sequence, number of repeated days, compliance etc.) in multiple 24-hour dietary recall method. Dietary intakes of college women in Seoul were obtained using 24-hour dietary recalls, consecutive 7 days, 4 times for 1 year. The result of this study shows that 1) the reproducibility of multiple 24-hour recalls is low(intraclass correlation coefficient<0.3), 2) the intraindividual variations of all nutrients are more than 80%, 3) the major components of variation are interindividual vriability and intraindividual variability(within person variability and methodologic error). There were small but statistically significant season and compliance effects. 4) the ratio within-person varation to between-person variation for absolute and transformed nutrient intakes except some nutrients such as vitamin A, vitamin C, energy from protein and fat etc, was less than 1.7 the minimum numbers of days needed in 24-hour dietary recalls to estimate usual intake for this group were 1 to 21 days. And the numbers of days needed to get the usual intake of an individual varied among individuals and within individuals for different nutrients, ranged from 1 days for log carbohydrate(% energy) to 470 days for Na. 6) There were greater than 0.7 correlation coefficients between the average nutrient intakes of 12 days and intakes of 28 days. Therefore, it was desirable to take the 24-hour recall more than 12 times, repeating 3 days every season.

• 한국수산과학회지
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• 제49권2호
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• pp.146-153
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• 2016

A feeding experiment was conducted to determine the nutritional value of various dietary protein sources without a vitamin premix supplementation in juvenile black rockfish, Sebastes schlegeli. Diets containing similar quantities of lipid and carbohydrate with 50% crude protein from fish meal (FM)/soybean meal (SBM)/corn gluten meal (CGM), FM only, casein/gelatin, high FM/low casein and low FM/high casein were fed to rockfish along with a commercial rockfish diet. The diet containing only 65.6% FM and the commercial rockfish diet produced significantly greater weight gain (WG) and feed efficiency (FE) than all other semipurified diets (SPDs); the economical diet with 52.8% FM, 10% SBM and 5% CGM produced lower WG than the diet containing FM and commercial rockfish diet, but FE and survival were similar both diets. Intermediate responses were observed for rockfish fed the SPD containing 32.8% FM and 27.4% casein, while SPDs containing 10% FM and 41.8% casein and 38.5% casein and 10% gelatin yielded the lowest WG and FE. Some differences in tissue indices and body composition of rockfish were induced by the diets containing various protein sources and levels without the vitamin premix supplementation.

• 한국식품영양학회지
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• 제35권2호
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• pp.159-166
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• 2022

The purpose of this study was to explore the marketability by developing a fresh HMR product for improving hyperlipidemia and dieting for elderly chronic disease patients and young people pursuing healthy beauty. The diet menu increased the ratio of fresh vegetables and mushrooms, and chicken breast and cheese were used as protein sources. By using whole grains, the supply of vitamins and minerals was strengthened while minimizing calories. Regarding the recipe, the diet menu was mainly prepared in the form of salads, risotto, and pasta. In the hyperlipidemia improvement menu, the proportion of fresh vegetables was increased, and as protein sources, pork shank, tofu, seafood, etc. with minimal fat were used. As a carbohydrate source, whole grains were mainly used to minimize calories while strengthening the supply of vitamins and minerals. In the recipe, it was prepared in the form of steamed or bibim-myeon, and it was also produced in a form such as paella. As a result, the developed menu was analyzed as low-calorie and evenly comprised of essential nutrients, which can satisfy palatability and nutrition.

• Asian-Australasian Journal of Animal Sciences
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• 제26권3호
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• pp.358-365
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• 2013

A series of in vitro studies were carried out to determine i) the effects of enzyme and formaldehyde treatment on the degradation characteristics of carbohydrate and protein sources and on the synchronicity of these processes, and ii) the effects of synchronizing carbohydrate and protein supply on rumen fermentation and microbial protein synthesis (MPS) in in vitro experiments. Untreated corn (C) and enzyme-treated corn (EC) were combined with soy bean meal with (ES) and without (S) enzyme treatment or formaldehyde treatment (FS). Six experimental feeds (CS, CES, CFS, ECS, ECES and ECFS) with different synchrony indices were prepared. Highly synchronous diets had the greatest dry matter (DM) digestibility when untreated corn was used. However, the degree of synchronicity did not influence DM digestibility when EC was mixed with various soybean meals. At time points of 12 h and 24 h of incubation, EC-containing diets showed lower ammonia-N concentrations than those of C-containing diets, irrespective of the degree of synchronicity, indicating that more efficient utilization of ammonia-N for MPS was achieved by ruminal microorganisms when EC was offered as a carbohydrate source. Within C-containing treatments, the purine base concentration increased as the diets were more synchronized. This effect was not observed when EC was offered. There were significant effects on VFA concentration of both C and S treatments and their interactions. Similar to purine concentrations, total VFA production and individual VFA concentration in the groups containing EC as an energy source was higher than those of other groups (CS, CES and CFS). The results of the present study suggested that the availability of energy or the protein source are the most limiting factors for rumen fermentation and MPS, rather than the degree of synchronicity.

• 한국식생활문화학회지
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• 제7권1호
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• pp.65-72
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• 1992

식생활은 지역, 경제, 문화 등의 다양한 요인에 의해 다른 양상을 나타낸다. 1960년 이후 한국의 경제는 급속도로 성장했고, 사회적으로도 많은 변화를 가져왔으며 한국인의 영양상태 또한 급격하게 변화하고 있다. 같은 동양권에서 한국과 일본의 식생활은 많은 유사점을 갖고 있으며 또한 시대별로 비슷한 경향의 식생활 변화를 나타내고 있다. 한국의 영양소별 섭취량은 1988년의 경우 탄수화물, 단백질은 일본을 앞서고 있으며, 지방은 일본이 한국에 비해 섭취량이 2배 이상 많았다. 에너지의 영양소별 섭취 구성비의 연차적 추이를 보면, 양국 모두 탄수화물의 섭취 비율은 감소하고 있고 단백질, 지방의 섭취비율은 증가하였다. 1988년의 경우 탄수화물, 단백질, 지방의 구성비가 일본은 59 : 15 : 26으로 적정수준에 도달해 있고, 한국은 67 : 19 : 14로 일본에 비해 탄수화물의 섭취 비율은 높고 지방의 섭취 비율은 낮은 것으로 나타났다. 식품 공급은 총량은 양국 모두 증가 추세이고, 공급 단백질 중 동물성 단백질이 일본은 45g, 한국은 30.7g이고, 공급 지질 중 유지류는 일본은 39g, 한국은 29g으로 동물성 식품과 유지류의 섭취가 한국에 비해 일본이 높았다.

• Asian-Australasian Journal of Animal Sciences
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• 제17권10호
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• pp.1400-1410
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• 2004

Eight crossbred (75% Holstein Friesian) cows in mid-lactation were randomly assigned to a switchback design with a 2x2 factorial arrangement to evaluate two nonstructural carbohydrate (NSC) sources (corn meal and cassava chips) with different rumen degradability and used at two levels of NSC (55 vs. 75%) with protein source (supplied by urea in the concentrate mix). The treatments were 1) Low degradable low level of corn (55%) 2) Low degradable high level of corn (75%) 3) High degradable low level of cassava (55%) and 4) High degradable high level of cassava (75%). The cows were offered the treatment concentrate at a ratio to milk yield at 1:2. Urea-treated rice straw was offered ad libitum as the roughage and supplement with 1 kg/hd/d cassava hay. The results revealed that total DM intake, BW and digestion coefficients of DM were not affected by either level or source of energy. Rumen fermentation parameters; NH3-N, blood urea nitrogen and milk urea nitrogen were unaffected by source of energy, but were dramatically increased by level of NSC. Rumen microorganism populations were not affected (p>0.05) by source of energy, but fungal zoospores were greater for cassava-based concentrate than corn-based concentrate. Milk production and milk composition were not affected significantly by diets containing either source or level of NSC, however concentrate than corn-based concentrate averaging (4.4 and 4.2, respectively). Likewise, income over feed, as estimated from 3.5% FCM, was higher on cassava-based concentrate than corn-based concentrate averaging (54.0 and 51.4 US$/mo, respectively). These results indicate that feeding diets containing either cassava-based diets and/or a higher of oncentrates up to 75% of DM with NPN (supplied by urea up to 4.5% of DM) can be used in dairy rations without altering rumen ecology or animal performance compared with corn-based concentrate.