• Title/Summary/Keyword: carbohydrate

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Production of Hydrolyzed Red Ginseng Residue and Its Application to Lactic Acid Bacteria Cultivation

  • Kim, Dong-Chung;In, Man-Jin
    • Journal of Ginseng Research
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    • v.34 no.4
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    • pp.321-326
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    • 2010
  • Enzymatic treatment conditions for red ginseng residue (RGR) were investigated to apply RGR as a microbial medium. Polysaccharide hydrolyase and protease were screened to obtain high solid and carbohydrate yields, and a good degree of carbohydrate hydrolysis. The optimal dosage and reaction time for Viscozyme, the chosen polysaccharide hydrolyase, were found to be 1.0% (w/w) and 3 h, respectively. Of the tested proteases, Flavourzyme, whose optimal dosage was 0.5% (w/w), was selected. Co-treatment with the optimal dosages of Flavourzyme and Viscozyme increased solid yield, carbohydrate yield, and degree of carbohydrate hydrolysis by 76%, 65%, and 1,865%, respectively, over levels in non-treated RGR. The culture characteristics of Leuconostoc mesenteroides strain KACC 91459P grown in enzymatically hydrolyzed red ginseng residue (ERGR) and RGR suspensions were compared. After cultivation for 6 h, the viable cell counts of both cell suspensions rapidly increased to $1.3{\times}10^9$ colony-forming units (CFU)/g. Moreover, while the viable cell population drastically decreased to $2.4{\times}10^6\;CFU/g$ for cells grown in RGR medium, it was maintained in cells fermented in ERGR medium for 24 h.

Macronutrient Intake and Obesity

  • Jamess W. DailyⅢ;Cha, Youn-Soo
    • Preventive Nutrition and Food Science
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    • v.5 no.1
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    • pp.58-64
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    • 2000
  • Obesity is a global pandemic that is increasing throughout most of the world. Increases in obesity are not restricted to highly industrialized countries, but have been observed in newly developed and developing countries as well. Obesity is associated with increased risk for non-insulin dependent diabetes mellitus, coronary artery disease, and some types of cancer. Tragically, eliminating food shortages in developing countries may result in substituting heart disease, diabetes, and cancer for malnutrition. There are many approaches to reducing obesity, including dietary modification, surgical interventions, and drug therapies. However, only dietary modification has the potential to be effective on a global scale. Public health measures in the United States have sought to reduce obesity by reducing the intake of dietary fat. While these efforts have succeeded in reducing dietary fat, obesity has continued to increase, suggesting that moderate fat reduction may not be effective. Other proposed diets include low-carbohydrate diets, low glycemic index diets, and very low fat diets. While all of these diets may be effective for some people, they are not satisfactory for public health policy. In fact, the ratio of fat to carbohydrate may not be as important as previously believed. Humans may be well suited to adapt to diets as varied as a high carbohydrate tropical diet consisting mostly of fruits to the high fat Eskimo diet consisting largely of animal foods. Either extreme may be healthful if providing adequate, but not excessive, energy and adequate amounts of micronutrients. Public health measures may need to focuss on reducing the overconsumption of inexpensive and convenient foods.

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Effect of Trichloroacetic Acid on the Solubility of Caseinomacropeptide

  • Shin, Sung-Chul;Jang, Hae-Dong
    • Preventive Nutrition and Food Science
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    • v.7 no.1
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    • pp.43-47
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    • 2002
  • Crude caseinomacropeptide (CMP) was prepared from Na-caseinate using a commercial renneting enzyme. Most of the crude CMP was released from the Na-caseinate by hydrolyzing with the enzyme for 40 min. The hydrolysis of the k-casein with carbohydrate was slower than that of the k-casein without carbohydrate, as shown by the analyses of the sialic acid content and the tricine-SDS-polyacrylamide gel electrophoresis. The yield of crude CMP from Na-caseinate was 3.7%. Cation exchange chromatography showed that the crude CMP consisted of 40.5% CMP and 59.5% caseinogylcomacropetide (CGP). The effect of the TCA concentration on the solubility of CMP and CGP was determined by using crude CMP. The amounts of crude CMP and sialic acid decreased in the proportion to the increase of trichloroacetic acid (TCA) concentration from 2 to 12%, suggesting that the CGP containing carbohydrate, as well as the CMP having no carbohydrate, was precipitated in a range of 4 to 12%, depending on the TCA concentration. This result supports the hypothesis that the different non-glycosylated and glycosylated forms of CMP have different sensitivities to TCA precipitation.

Effects of Garcinia cambogia Extract Feeding on Body Weight and Lipid Profiles in Rats Fed a High-carbohydrate or High-fat Diet

  • Hong, So-Young;Park, Ju-Yeon;Sohn, Jung-Sook;Kim, Joo-Hee;Kim, Mi-Kyung
    • Food Science and Biotechnology
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    • v.18 no.3
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    • pp.649-654
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    • 2009
  • This study was designed to evaluate the effects of high-carbohydrate (HC) or high-fat (HF) diet with hydroxycitric acid (HCA)-containing Garcinia cambogia in rats. Seventy male Sprague-Dawley rats were randomly divided into 7 groups and raised with experimental diets containing different HCA levels (0, 1.6, and 3.2%) and calorie sources (carbohydrate, fat) for 8 weeks. Energy intake was significantly reduced in rats fed a HC diet with HCA compared to the HC diet without HCA. Body weight gain was significantly reduced in HCA groups. In the diet groups, plasma total lipid and triglyceride (TG) levels of the HCA groups were significantly lower than those of the group without HCA. There were no significant differences in energy intake and plasma lipid profile in HF groups. These results suggest that HC diet with HCA was more effective in the reduction of energy intake, body weight gain, and plasma lipid contents than those of HF diet with HCA.

Ethanol Effect of Nelumbo nucifera Root on Carbohydrate Methabolism Related Enzyme Activities and Antioxidative Effect in Streptozotocin-Induced Diabetic Rats (Streptozotocin으로 유발된 당뇨 흰쥐에 대한 연근 에탄올 추출물의 당대사 효소활성과 항산화 작용에 미치는 영향)

  • Kim, Ok-Kyung
    • Journal of the Korean Applied Science and Technology
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    • v.31 no.3
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    • pp.509-516
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    • 2014
  • This study was done to investigate the carbohydrate metabolism related enzyme activities and antioxidative effects of Nelumbo nucifera(N.N) Root in streptozotocin (STZ)-induced diabetic rats. The contents of serum glucose, triglyceride (TG) and Total cholesterol were significantly decreaed in N.n treated group compared to the those of STZ-control group. The activity of glucose-6-pase(G-6-Pase) was significantly decreased in N.N treated group. Also the activity of glucokinase(Gk) was significantly increaed in N.N treated group. The content of hepatic glycogen was significantly increaed in N.N treated group, in addition, content of malondialdehyde(MDA) was significanly decreased in N.N treated group. Also, content of glutathione(GSH) was significanly increased in N.N treated froup. whereas, activity of catalase(CAT) was significantly decreaed in N.N treated group compared to the those of STZ-control group. activity of glutathione peroxidase(GSH-Px) was inecreaed. In conclusion, these results indicated that ethanol extract of N.N would have carbohydrate metabolism antioxidative effects in STZ-induced diabetic rats.

Effects of oral caffeine and capsaicin administration on energy expenditure and energy substrates utilization in resting rats

  • Kim, Jisu;Jeon, Yerim;Hwang, Hyejung;Suh, Heajung;Lim, Kiwon
    • Korean Journal of Exercise Nutrition
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    • v.15 no.4
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    • pp.183-189
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    • 2011
  • Caffeine and capsaicin increase resting energy metabolism. However, most measurements have been conducted in short-term studies. Therefore, we investigated the effects of caffeine and capsaicin on energy expenditure and energy substrate utilization in resting rats for 6 h. The caffeine (Experiment 1) experiment included four male rats aged 5 weeks and measured the effects of oral administration of caffeine (10 or 50 mg/kg) on respiratory gas, energy expenditure, and energy substrate oxidation for 6 h. Experiment 2 included four male rats aged 6 weeks to measure the effects of capsaicin (10 mg/kg) using the same method as in Experiment 1. The results of Experiment 1 indicated that O2 uptake and carbohydrate oxidation after caffeine administration for 2 h was higher in the 10 mg trial than that in the 50 mg or placebo trials (P < 0.05). However fat oxidation was not significantly different. In contrast, capsaicin (Experiment 2) observed no differences between the placebo and the capsaicin trials. In conclusion, caffeine initially increased the resting energy consumption for 2 h, and this energy expenditure was due to carbohydrate oxidation. Capsaicin did not change oxygen uptake, respiratory exchange ratio, fat oxidation, or carbohydrate oxidation.

Effect of Suboptimal Nutritional Status on Mineral Uptake and Carbohydrate Metabolism in Tomato Plants

  • Sung, Jwakyung;Lee, Sangmin;Lee, Suyeon;Kim, Rogyoung;Lee, Yejin;Yun, Hongbae;Ha, Sangkeun;Song, Beomheon
    • Korean Journal of Soil Science and Fertilizer
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    • v.46 no.5
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    • pp.351-358
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    • 2013
  • A suitable supply of mineral elements into shoot via a root system from growth media makes plants favorable growth and yield. The shortage or surplus of minerals directly affects overall physiological reactions to plants and, especially, strongly influences carbohydrate metabolism as a primary response. We have studied mineral uptake and synthesis and translocation of soluble carbohydrates in N, P or K-deficient tomato plants, and examined the interaction between soluble carbohydrates and mineral elements. Four-weeks-old tomato plants were grown in a hydroponic growth container adjusted with suboptimal N ($0.5mmol\;L^{-1}\;Ca(NO_3)2{\cdot}4H_2O$ and $0.5mmol\;L^{-1}\;KNO_3$), P ($0.05mmol\;L^{-1}\;KH_2PO_4$), and K ($0.5mmol\;L^{-1}\;KNO_3$) for 30 days. The deficiency of specific mineral element led to a significant decrease in its concentration and affected the concentration of other elements with increasing treatment period. The appearance of the reduction, however, differed slightly between elements. The ratios of N uptake of each treatment to that in NPK sufficient tomato shoots were 4 (N deficient), 50 (P deficient), and 50% (K deficient). The P uptake ratios were 21 (N deficient), 19 (P deficient), and 28% (K deficient) and K uptake ratios were 11 (N deficient), 46 (P deficient), and 7% (K deficient). The deficiency of mineral elements also influenced on carbohydrate metabolism; soluble sugar and starch was substantially enhanced, especially in N or K deficiency. In conclusion, mineral deficiency leads to an adverse carbohydrate metabolism such as immoderate accumulation and restricted translocation as well as reduced mineral uptake and thus results in the reduced plant growth.

Ionizing Radiation Effect on the Carbohydrate Moiety of Chicken Ovomucoid (계란 ovomucoid의 탄수화물 부분에 미치는 이온화방사선의 영향)

  • Lee, Young-Keun;Kim, Jin-Kyu;Kim, Jae-Sung;Song, Hi-Sup;Charoen, Saovapong;Amornraksa, Kitti
    • Journal of Radiation Protection and Research
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    • v.22 no.1
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    • pp.23-27
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    • 1997
  • Radiation effects on carbohydrate moiety of chicken ovomucoid, a protease inhibitor as a typical allergenic glycoprotein of egg white, was observed. The trypsin inhibitory activity of chicken ovomucoid decreased exponentially and the inactivation was more significant irradiated in $N_2$ than in $O_2$. From the protein blotting, radiation caused protein degradation in $O_2$ and protein aggregation also in $N_2$. The patterns of carbohydrate blotting were also similar with that of protein blotting. Sugar chains in low molecular weight fraction (MW<5,000) were released by radiation and those in $O_2$ were higher than in $N_2$. From the HPLC patterns of the degradation of sugar chains, all peaks of oligosaccharides have the tendency to decrease with the increase of radiation dose and more remarkable in $O_2$ than in $N_2$. These results suggest that ionising radiation could cause the overall conformational changes of ovomucoid by the degradation and release of oligosaccharides.

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Nutritional Evaluation of Tofu Containing Dried Soymilk Residue(DSR) 2. Evaluation of Carbohydrate Quality (건조비지 첨가 두부의 영양적 품질평가 2. 탄수화물의 품질)

  • Kweon, Mi-Na;Ryu, Hong-Soo;Mun, Sook-Im
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.22 no.3
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    • pp.262-265
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    • 1993
  • Dietary fiber content and carbohydrate digestibility of dried soymilk residue (DSR) and tofu containing DSR were evaluated. Insoluble dietary fiber (IDF) content was 37.4 and 49.8% (%, moisture free basis) for common soymilk residue and DSR, respectively. Both soymilk residues contained 12.5% of soluble dietary fiber (SDF, dry basis). Tofu containing DSR, which is partially substituted with DSR corresponding to 10% weight of soybean used, had higher dietary fiber content (30% more for RDF and 45% more for SDF) than tofu manufactured in traditional manner. Carbohydrate digestibility was much lower in all tofu products ranging from 11% to 21%, and there was a negative correlation( r = -0.9243) between carbohydrate digestibility and total dietary fiber content.

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The Effect of Carbohydrate Processed Snacks on Middle School Students' Emotional Intelligence (경기 일부 중학생의 당류 간식 섭취가 정서지능에 미치는 영향)

  • Lee, Jin-Kyoung;Rhie, Seung-Gyo
    • The Korean Journal of Community Living Science
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    • v.19 no.3
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    • pp.335-351
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    • 2008
  • Adequate nutrients intake and regular eating habit are so important for the students to develop physical and mental health, and they contribute significantly for the development of intellectual capacity. The intake of simple sugar contained in the processed foods is known to have been related not only with such physical problems as obesity and diabetes but also with hyperactivity during growing period. This study analysed the effects of carbohydrate processed snacks on the middle school students' emotional intelligence and intended to demonstrate the correlation and the influence of the foods. The subjects of this study were 476 the 1st grade middle school students sampled from two public middle schools of Gyeonggi Province, Korea. Questionnaire contained 47 questions including the kinds of sugar containing processed foods, the frequency of intake, the frequency of normal diet, and the emotional test. All data obtained in this study were processed by SAS 8.2 program. 77.9% of the students had snacks more than once a day and the favored foods were in the order of bread, cookies, fruit, milk and dairy products, flour foods, ramen and ice cream. Less of the sugar contained processed snacks consumed high emotional intelligence showed(p<0.001). And low intake of carbohydrate processed snacks linked with having more regular meals and the higher levels of emotional intelligence. There was no gender difference in consuming simple sugar containing processed foods, however, out of these foods, carbonated drinks, ramen and pizza affected significantly the emotional intelligence of the male students. It was found that sugar contained processed snacks affect the eating habits like meal skipping and the emotional intelligence. It is determined that the effects of carbohydrate processed snacks on emotional intelligence can not be denied.

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