• 한국임상수의학회지
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• 제32권1호
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• pp.41-44
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• 2015

이 연구는 더러브렛 말에서 호이산화탄소성 Streptococcus equi subsp equi 에 기인한 선역의 발생에 관한 보고이다. 3개의 다른 농장에서 선역의 전형적인 증상인 림프절 화농이 있거나 없는 29두의 말에서 17 균주의 S equi subsp equi를 분리하였다. 이들 중 임상증상이 있는 1두에서 분리한2균주는 호기배양에서 분리되었으나, 임상증상이 있는 6두에서 분리한 7균주와 임상증상이 없는 22두에서 분리한 8균주는 호기조건에서 배양되지 않았다. 이 15 분리균주는 일반 호기배양기에서는 증식하지 않았으며, 5% $CO_2$배양기, 산소분압을 낮추는 candle jar 및 GasPak을 이용한 혐기배양에서 증식하는 호이산화탄소성, 산소민감성 또는 이산화탄소 요구성 S. equi subsp. equi로 판명되었다. 이 연구는 호이산화탄소성 S. equi subsp. equi 에 의한 선역발생에 관한 최초의 보고이다.

• Journal of Microbiology
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• 제43권1호
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• pp.28-33
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• 2005

The purpose of this study was to develop a multipurpose incubator, without the gas cylinders (bottles) which are required for $H_2$ and $CO_2$ supplementation. In our bottle-free multipurpose incubator, the $H_2$ and $CO_2$ were generated by chemical reactions induced within the chamber. The reaction between sodium borohydride and acetic acid at a molar ratio of 1:1 was used to generate $H_2$, according to the following formula: $4NaBH_4+2CH_3COOH+7H_2O{\rightarrow} 2CH_3COONa+Na_2B_4O_7+16H_2$, whereas the other reaction, citric acid and sodium bicarbonate at a 1:1 molar ratio, was used to generate $CO_2$, according to the following formula: $C_6H_8O_7+3NaHCO_3{\rightarrow}Na_3(C_6H_5O_7)+3H_2O+3CO_2$. Five species of obligate anaerobic bacteria, one strain of capnophilic bacterium, and one strain of microaerophilic bacterium were successfully cultured in the presence of their respective suitable conditions, all of which were successfully generated by our bottle-free multipurpose incubator. We conclude that, due to its greater safety, versatility, and significantly lower operating costs, this bottle-free multipurpose incubator can be used for the production of fastidious bacterial cultures, and constitutes a favorable step above existing anaerobic incubators.

• Journal of Microbiology and Biotechnology
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• 제23권4호
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• pp.451-458
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• 2013

The human gastric pathogen Helicobacter pylori (Hp) has been considered a microaerophile. However, we recently reported that, when supplied with 10% $CO_2$, Hp growth is stimulated by an atmospheric level of $O_2$, suggesting that Hp is a capnophilic aerobe. In this study, we investigated the effects of aerobic $O_2$ tension on Hp cells by comparing gene expression profiles of cultures grown under microaerobic and aerobic conditions in the presence of 10% $CO_2$. The results showed that overall differences in gene expression in Hp cells grown under the two $O_2$ conditions were predominantly growth-phase-dependent. At 6 h, numerous genes were down-regulated under the aerobic condition, accounting for our previous observation that Hp growth was retarded under this condition. At 36 h, however, diverse groups of genes involved in energy metabolism, cellular processes, transport, and cell envelope synthesis were highly up- or down-regulated under the aerobic condition, indicating a progression of the cultures from the log phase to the stationary phase. The expression of several oxidative stress-associated genes including tagD, katA, and rocF was induced in response to aerobic $O_2$ level, whereas trxA, trxB, and ahpC remained unchanged. Altogether, these data demonstrate that aerobic $O_2$ tension is not detrimental to Hp cells but stimulates Hp growth, supporting our previous finding that Hp may be an aerobic bacterium that requires a high $CO_2$ level for its growth.