• Title/Summary/Keyword: candida

Search Result 1,253, Processing Time 0.03 seconds

Production of Glutathione by Candida sp. Mutant (Candida sp. 변이주에 의한 Glutathione 생산)

  • 김대선;유재홍;신원철;윤성식
    • Microbiology and Biotechnology Letters
    • /
    • v.21 no.5
    • /
    • pp.435-439
    • /
    • 1993
  • For the overproduction of glutathione, Candida sp. mutant was isolated by the treatment with U.V. light. The highest glutathione production of Candida sp. mutant was obtained after shaking culture for 48 hours in the cullture medium containing glucose 1.5%(w/v), yeast extract 4.0% (w/v), KH2PO4 0.04%(w/v), biotin 5 ng/ml, and L-cysteine 0.04%(w/v). The optimal pH and temperature for the glutathione production were pH 6.0 and 25C, respectively.

  • PDF

An infant with a palatal fistula secondary to Candida infection

  • Sharma, Sarwpriya;Chauhan, Jaideep Singh
    • Archives of Craniofacial Surgery
    • /
    • v.21 no.3
    • /
    • pp.206-209
    • /
    • 2020
  • Candida osteomyelitis affecting maxillofacial bones has been scantly documented in the literature. Infantile osteomyelitis is an uncommon and life-threatening disease. Candida osteomyelitis causes significant morbidity. The present report describes a case of a 9-month-old infant with infantile osteomyelitis secondary to candida infection. This report describes its presentation and the management of palatal fistula in an infant.

Correlative Relationship between Proteinase, Phospholipase Activity and Adherence to Buccal Epithelial Cells of Experimental Strains of Candida albicans (Candida albicans의 Proteinase 및 Phospholipase 분비능과 구강상피세포 부착능과의 상호관계)

  • Koh, Choon-Myung;Kim, Soo-Ki
    • The Journal of the Korean Society for Microbiology
    • /
    • v.22 no.4
    • /
    • pp.403-411
    • /
    • 1987
  • This study investigated whether a correlation exists between proteinase activity, phospholipase activity and adherence of Candida albicans to buccal epithelial cells by using of various strains isolated from oral cavity. The proteinase activity of 30 strains was tested by culture on agar media that contained bovine serum albumin as a nitrogen source. Using the serum-protein-agar method to test proterolysis of serum albumin in 20 strains of Candida albicans. Twenty-six strains of Candida albicans were phospholipase producers and the degree of phopholipase activity of experimental strains were $0.51{\sim}0.89$ measured by Pz-value. Twenty-eight strains of Candida albicans were adhersive to buccal epithelial cells and 15 strains were foung significantly active adherence. Fifteen strains of Candida albicans were correlated with proteinase activity and adherence to epithelial cells and concomitantly 20 strains of Candida albicans were also correlated with phospholipase activity and adherence. In conclusion our investigation provides evidence of a correlation between quantitative proteinase, phospholipase and adherence. An association of these parameters may be an important contributory factor for pathogenicity.

  • PDF

The build angle of 3D printing denture base resin on candida albicans adhesion. (의치상레진의 3D 프린팅 출력 각도가 Candida albicans의 부착에 미치는 영향)

  • Park, Su-Jung;Song, Young-Gyun
    • The Journal of the Korean dental association
    • /
    • v.58 no.1
    • /
    • pp.19-26
    • /
    • 2019
  • Purpose: The purpose of this study is to compare the adhesion of Candida albicans according to build angle in 3D printing denture base resin. Methods: The 3D printing was performed by setting the build angle of the disk type specimen designed by CAD program at 0 degree, 30 degrees, 60 degrees, and 90 degrees. Surface roughness was measured using a non-contact 3D microsurface profiler. The specimens were incubated in Candida albicans suspension for 24 hours. The attached Candida albicans were detached by cell scraper. The suspension of detached C. albicans was serially diluted and plated on Trypticase soy broth. After 48 hours of incubation, total colony forming unit was counted. Results: There was no significant difference in surface roughness(Sa) between the test groups, but the interlayer boundary was observed. There was no statistically significant difference in total colony forming units of Candida albicans between the test groups. Conclusion: There was no difference in the average surface roughness and adhesion of Candida albicans between the specimens. It is considered that the setting of the build angle should be set considering the accuracy or strength rather than the roughness of the surface.

  • PDF

Antifungal Effects of Cinamon Ramulus, Pulsatillae Radix, Dictamni Radicis Cortex, Paeoniae Radix, Arecae Semen, Artemisiae Capillaries Herba against Candida albicans (Candida albicans에 대한 계지(桂枝), 백두옹(白頭翁), 백선피(白鮮皮), 백작약(白芍藥), 빈낭, 인진(茵蔯)의 항진균효과)

  • Choi, In-Ho;Kim, Yeon-Hee;Lee, Dong-Nyung;Kim, Hyung-Jun
    • Journal of Physiology & Pathology in Korean Medicine
    • /
    • v.19 no.3
    • /
    • pp.690-695
    • /
    • 2005
  • The purpose of this thesis is to dedicate to get the objectivity of herbal medicine treatments by choosing herbs likely to work as antifungal agents against Candida albicans which is the causes of Candida vaginitis, making experiments on them and getting the significant results. Each herb's efficacy on control the number of Candida vaginitis is noticed by using Disk Susceptibility test with six herbs medicine and Broth dilution assay of the culture. Cinamon Ramulus, Pulsatillae Radix, Dictamni Radicis Cortex, Paeoniae Radix, Arecae Semen, Artemisiae Capillaries Herba extracts experimented on the efficiency of antifungal activities against Candida albicans by disk susceptibility test. Pulsatillae Radix has the strongest efficiency. In Dictamni Radicis Cortex never appeared antifungal effect on treatment. MIC (minimal inhibition concentration) in vitro antifungal agents of Cinamon Ramulus, Pulsatillae Radix, Paeoniae Radix, Artemisiae Capillaries Herba extracts against Candida albicans was determined by broth dilution assay. MIC against Candida albicans is that Pulsatillae Radix is 2.5 mg/ml and Cinamon Ramulus is 5 mg/ml and Paeoniae Radix is 10 mg/ml and Artemisiae Capillaries Herba is 40 mg/ml.

Isolation of Wild Yeasts from Soils of Reed Fields in Seocheon-gun County, Chungcheongnam-do, South Korea, and Characterization of Unrecorded Yeasts (충남 서천군 갈대밭 주변 토양에서 야생효모의 분리 및 미기록종 특성)

  • Han, Sang-Min;Lee, Sang-Yeop;Lee, Hyang-Burm;Lee, Jong-Soo
    • The Korean Journal of Mycology
    • /
    • v.45 no.3
    • /
    • pp.234-240
    • /
    • 2017
  • The goal of this study was to isolate wild yeasts from reed fields in the Seocheon-gun county in Chungcheongnam-do, South Korea. Molecular analysis of the D1/D2 domain of the 26S rDNA of the yeasts isolated from soil samples was performed using Basic Local Alignment Search Tool (BLAST). In total, 20 yeast strains from 11 species were isolated from 20 different soil samples. Candida species were observed to be the most common yeasts. Of these 20 strains, Candida subhashii (6 strains), Candida tropicalis (3 strains), Candida boleticola MS008, and Lachancea thermotolerans (2 strains) were identified as alcohol-fermenting yeasts. Further, Bullera japonica YJ10-1, Candida subhashii J7-1, Kluyveromyces yarrowii YJ11-1, and Ustilago shanxiensis Y10-1 were newly recorded yeast strains in Korea, and therefore, their microbiological characteristics were investigated further. All of these unrecorded yeast strains had oval cells; only Candida subhashii J7-1 formed ascospores and pseudomycelia. Kluyveromyces yarrowii YJ11-1 grew in vitamin-free medium, and all of these strains, except Candida subhashii J7-1, grew in 5% NaCl-containing YPD broth.

Microbiological Characteristics and Physiological Functionality of Unrecorded Yeasts from Mountains Soils in Daejeon Metropolitan City and Chungcheongnam-do, Korea (대전광역시와 충청남도 산림토양에서 분리한 국내 미기록 효모들의 미생물학적 특성과 생리기능성)

  • Han, Sang-Min;Lee, Jong-Soo
    • The Korean Journal of Mycology
    • /
    • v.44 no.3
    • /
    • pp.138-144
    • /
    • 2016
  • Twelve unrecorded yeasts, Pseudozyma prolifica HL9-1, Trichosporon coremiiforme NS19-2, Candida cretensis SA4-1, Cryptococcus diffluens TJ4-3, Cryptococcus pinus YB17-2, Candida vartiovaarae DD2-5, Pichia galeiformis DM3-5, Candida pseudolambica JW2-3, Trichosporon xylopini NS5-1, Trichosporon moniliiforme NS5-7, Tetrapisispora iriomotensis NS14-2, and Tetrapisispora nanseiensis SA17-1, were screened among 97 yeasts from soils of Chungcheongnam-do and Daejeon metropolitan city, Korea. These yeasts were oval or ellipsoidal and had a budding system for vegetative reproduction. They grew well in yeast extract-peptone-dextrose (YPD) medium and, in particular, Tetrapisispora iriomotensis NS14-2 and Candida cretensis SA4-1 grew well in 10% NaCl-containing YPD broth. Nine strains, including Trichosporon coremiiforme NS19-2, assimilated xylose and four yeast strains, such as Candida vartiovaarae DD2-5, also assimilated lactose. Physiological functionalities of cell-free extracts and supernatants from two halophilic unrecorded yeasts, Candida cretensis SA4-1 and Tetrapisispora iriomotensis NS14-2, were investigated. Cell-free extracts from Candida cretensis SA4-1 and Tetrapisispora iriomotensis NS14-2 exhibited 71.3% and 68.4% antihypertensive angiotensin I-converting enzyme inhibitory activity.

Combined Effects of the Essential Oil from Eucalyptus globulus with Ketoconazole against Candida and Trichophyton Species

  • Lim, Sook;Shin, Seung-Won
    • Natural Product Sciences
    • /
    • v.14 no.1
    • /
    • pp.27-31
    • /
    • 2008
  • We have evaluated the combined antifungal effects of the essential oil from Eucalyptus globulus or its main component 1,8-cineole with ketoconazole. Checkerboard microtiter tests were used to analyze their effects against three Candida and six Trichophyton species. The susceptibility of the Trichophyton species to E. globulus essential oil differed distinctly. The fractional inhibitory concentration indices (FICIs) against the tested Candida species ranged between 0.09 and 0.38 for ketoconazole combined with E. globulus essential oil or 1,8-cineole, indicating significant synergism between ketoconazole and the oil samples. Similar experiments using Trichophyton species resulted in FICIs between 0.28 and 0.63, indicating relatively weaker combined effects than those observed with Candida species. Thus, the data reported here show that the anti-Candida effects of ketoconazole can be significantly improved in the presence of E. globulus essential oil or 1, 8-cineole.

Antimicribial Photodynamic Therapy Using Diode Laser on Candida Albicans (다이오드 레이저를 이용한 칸디다 알비칸스에 대한 항균 광역학 요법)

  • Kim, Ji-Won;Koo, Bon-Yeoul
    • Journal of radiological science and technology
    • /
    • v.44 no.2
    • /
    • pp.141-146
    • /
    • 2021
  • This study aimed to explore whether photodynamic therapy using Radachlorin and diode laser is an effective inhibitor of Candida albicans. Suspensions of Candida albicans were obtained, inoculated in petri dishes with Radachlorin, and incubated for 30 min. Then, the laser light of a diode laser was irradiated at at energy densities of 3 J/cm2, 5 J/cm2, 7 J/cm2. As a result, Candida albicans showed a killing rate of 91.5% at an energy density of 7 J/cm2. This study found that photodynamic therapy using a Radachlorin and diode laser was effective for the inhibition of Candida albicans.