Browse > Article
http://dx.doi.org/10.4489/KJM.20170028

Isolation of Wild Yeasts from Soils of Reed Fields in Seocheon-gun County, Chungcheongnam-do, South Korea, and Characterization of Unrecorded Yeasts  

Han, Sang-Min (Department of Biomedicinal Science and Biotechnology, Paichai University)
Lee, Sang-Yeop (Department of Biomedicinal Science and Biotechnology, Paichai University)
Lee, Hyang-Burm (Division of Food Technology, Biotechnology and Agrochemistry, College of Agriculture and Life Sciences, Chonnam National University)
Lee, Jong-Soo (Department of Biomedicinal Science and Biotechnology, Paichai University)
Publication Information
The Korean Journal of Mycology / v.45, no.3, 2017 , pp. 234-240 More about this Journal
Abstract
The goal of this study was to isolate wild yeasts from reed fields in the Seocheon-gun county in Chungcheongnam-do, South Korea. Molecular analysis of the D1/D2 domain of the 26S rDNA of the yeasts isolated from soil samples was performed using Basic Local Alignment Search Tool (BLAST). In total, 20 yeast strains from 11 species were isolated from 20 different soil samples. Candida species were observed to be the most common yeasts. Of these 20 strains, Candida subhashii (6 strains), Candida tropicalis (3 strains), Candida boleticola MS008, and Lachancea thermotolerans (2 strains) were identified as alcohol-fermenting yeasts. Further, Bullera japonica YJ10-1, Candida subhashii J7-1, Kluyveromyces yarrowii YJ11-1, and Ustilago shanxiensis Y10-1 were newly recorded yeast strains in Korea, and therefore, their microbiological characteristics were investigated further. All of these unrecorded yeast strains had oval cells; only Candida subhashii J7-1 formed ascospores and pseudomycelia. Kluyveromyces yarrowii YJ11-1 grew in vitamin-free medium, and all of these strains, except Candida subhashii J7-1, grew in 5% NaCl-containing YPD broth.
Keywords
Alcohol-fermenting yeasts; Reed field; Soils; Unrecorded yeasts; Wild yeast;
Citations & Related Records
Times Cited By KSCI : 16  (Citation Analysis)
연도 인용수 순위
1 Han SM, Lee JS. Isolation and identification of wild yeasts from soils of an herb park in Seoul metropolitan city and characteristics of unrecorded yeasts. Kor J Mycol 2016;44:108-12.
2 Han SM, Lee SY, Kim HK, Lee JS. Characterization of wild yeasts isolated from leaves obtained from Mt. Daedun and Mt. Chilgap, Korea. Kor J Mycol 2017;45:31-42.
3 Han SM, Lee JS. Characterization of unrecorded yeasts Isolated from leaves of trees of Oknyeobong peak and Yeonjasan mountain in Daejeon, Korea. Kor J Mycol 2017;45:23-30.
4 Han SM, Kim HK, Lee HB, Lee JS. Isolation and identification of wild yeasts from freshwaters and soils of Nakdong and Yeongsan river, Korea, with characterization of two unrecorded yeasts. Kor J Mycol 2016;44:350-4.
5 Hyun SH, Han SM, Kim HK, Lee JS. Yeast diversity of wild flowers in mountains of Korea and their physiological functionalities. Kor J Mycol 2015;43:137-41.
6 Han SM, Jeon SJ, Lee HB, Lee JS. Screening of -aminobutyric acid (GABA)-producing wild yeasts and their microbiological characteristics. Kor J Mycol 2016;44:87-93.
7 Song JH, Baek SY, Lee DH, Jung JH, Kim HK, Lee JS. Screening of fungal Nuruk and yeast for brewing of Gugija-Liriope tuber traditional rice wine and optimal fermentation condition. Kor J Mycol 2011;39:78-84.   DOI
8 Lee DH, Kang HY, Lee YS, Cho CH, Kim SJ, Lee JS. Effects of yeast and Nuruk on the Quality of Korean Yakju. Korean J Microbiol Biotechnol 2011;39:274-80.
9 Kim JH, Kim NM, Lee JS. Physiological characteristics and ethanol fermentation of thermotolerant yeast Saccharomyces cerevisiae OE-16 from taditional Meju. Korean J Food Nutr 1999;12:490-5.
10 Sampaio JP, Inacio J, Fonseca A, Gadanho M, Spencer-Martins I, Scorzetti G, Fell JW. Auriculibuller fuscus gen. nov., sp. nov. and Bullera japonica sp. nov., novel taxa in the Tremellales. Int J Syst Evol Microbiol 2004;54:987-93.   DOI
11 Hilber-Bodmer M, Schmid M, Ahrens CH, Freimoser FM. Competition assays and physiological experiments of soil and phyllosphere yeasts identify Candida subhashii as a novel antagonist of filamentous fungi. BMC Microbiol 2017;17:4.   DOI
12 Belloch C, Querol A, García MD, Barrio E. Phylogeny of the genus Kluyveromyces inferred from the mitochondrial cytochrome-c oxidase II gene. Int J Syst Evol Microbiol 2000;50:405-16.   DOI
13 Fukuoka T, Morita T, Konishi M, Imura T, Kitamoto D. Characterization of new types of mannosylerythritol lipids as biosurfactants produced from soybean oil by a basidiomycetous yeast, Pseudozyma shanxiensis. J Oleo Sci 2007;56:435-42.   DOI
14 Hyun SH, Min JH, Lee HB, Kim HK, Lee JS. Isolation and diversity of yeasts from wild flowers in Ulleungdo and Yokjido, Korea. Kor J Mycol 2014;42:28-33.   DOI
15 Lee JS, Yi SH, Kwon SJ, Ahn C, Yoo JY. Enzyme activities and physiological functionality of yeasts from traditional Meju. Korean J Appl Microbiol Biotechnol 1997;25:448-53.
16 Min JH, Ryu JJ, Kim HK, Lee JS. Isolataion and identification of yeasts from wild flowers in Gyejoksan, Oseosan and Baekamsan of Korea. Kor J Mycol 2013;41:47-51.   DOI
17 Hyun SH, Mun HY, Lee HB, Kim HK, Lee JS. Isolation of yeasts from wild flowers in Gyonggi-do province and Jeju island in Korea and the production of anti-gout xanthine oxidase inhibitor. Korean J Microbiol Biotechnol 2013;41:383-90.   DOI
18 Hyun SH, Lee JK, Park WJ, Kim HK, Lee JS. Isolation and diversity of yeasts from fruits and flowers of orchard in Sinam-myeon of Yesan-gun, Chungcheongnam-do, Korea. Kor J Mycol 2014;42:21-7.   DOI
19 Hyun SH, Han SM, Lee JS. Isolation and physiological functionality of yeasts from wild flowers in Seonyudo of Gogunsanyeoldo, Jeollabuk-do, Korea. Kor J Mycol 2014;42:201-6.   DOI
20 Han SM, Hyun SH, Lee JS. Isolation and identification of yeasts from wild flowers in Deogyu mountain and their physiological functionalities. Kor J Mycol 2015;43:47-52.   DOI
21 Han SM, Han JW, Bae SM, Park WJ, Lee JS. Isolation and identification of wild yeasts from soils of paddy fields in Daejeon metropolitan city and Chungcheongnam-do, Korea. Kor J Mycol 2016;44:1-7.   DOI
22 Han SM, Hyun SH, Lee HB, Lee HW, Kim HK, Lee JS. Isolation and identification of yeasts from wild flowers collected around Jangseong lake in Jeollanam-do, Republic of Korea, and characterization of the unrecorded yeast Bullera coprosmaensis. Mycobiology 2015;43:266-71.   DOI