• Journal of Korean Society of Forest Science
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• v.97 no.5
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• pp.492-500
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• 2008

Since vegetation significantly influences on soil carbon and nutrient storage, vegetation change has been focused on terrestrial carbon and nutrient cycling studies. In this study we investigated soil carbon and major nutrient capitals at the abandoned fields, which had different vegetation composition: a three year abandoned field ($AGR_3$), two ten years abandoned fields ($PD_{10}$ dominant with Pinus densiflora and Fraxinus rhynchophylla and $PM_{10}$ dominant with Populus maximowiczii), and an over sixty years forest ($FOR_{60}$). which were located at Hongcheon-gun, Kangwon-do, South Korea. Both main effects for organic matter (%) were significant: shallow soil > deep soil and $FOR_{60}=PM_{10}$ > $AGR_3=PD_{10}$. Nitrogen concentrations at $PM_{10}$ were the highest, while the lowest at $PD_{10}$. Available phosphorus concentrations were the highest at $PD_{10}$, which were over 10 times of site $FOR_{60}$ and $AGR_3$ at 0-10 cm soil depth. The average organic matter ($173Mg\;ha^{-1}$) and nitrogen contents ($10Mg\;ha^{-1}$) of $PM_{10}$ and $FOR_{60}$ were higher than those of $AGR_3$ and $PD_{10}$ by 57% and 42%, respectively. The available phosphorus contents above 30 cm mineral soil at $PD_{10}$ ($3.8Mg\;ha^{-1}$) and $PM_{10}$ ($1.3Mg\;ha^{-1}$) were over 120 times and 40 times more than at $FOR_{60}$. Calcium ($3.7Mg\;ha^{-1}$) and magnesium contents ($2.8Mg\;ha^{-1}$) at $FOR_{60}$ were twice or three times higher than at other sites. Organic matter amounts in 0-10 cm and 10-30 em soil had significant positive relationships with nitrogen, calcium, and magnesium contents, but not available phosphorus and potassium contents. This study could not identify the effect of chronological factor and vegetation composition on soil carbon and nutrient capital owing to diverse topography as well as limited study sites. However, this study suggests the accuracy of investigation for regional carbon and nutrient sequestration can be achieved by considering the period of abandoned time on the fields and the land use types. These results may suggest the benefits of forest restoration for soil carbon and nutrient accumulation in marginal agricultural lands in South Korea.

• Korean Journal of Veterinary Research
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• v.39 no.1
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• pp.34-44
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• 1999

Ischemic damage in the selectively vulnerable populations of neurons is thought to be caused by an abnormal accumulation of intracellular calcium. It has been reported that the neurons, expressing specific calcium binding proteins, might effectively control intracellular calcium concentrations because of a high capacity to buffer intracellular calcium in the brain ischemic condition. It is uncertain that parvalbumin, one of the calcium binding proteins, can protect the neurons from the cerebral ischemic damage. Recently, treatment of kappa opioid agonists increased survival rate, improved neurological function, and decreased tissue damage under the cerebral ischemic condition. Many evidences indicate that these therapeutic effects might result from regulation of calcium concentration. This study was designed to analyze the changes of number in parvalbumin-positive neurons after cerebral ischemic damage according to timepoints after cerebral ischemic induction. In addition, we evaluated the effect of GR89696 (kappa opioid agonist) or naltrexone(non selective opioid antagonist) on the changes of number in parvalbumin expressing neurons under ischemic condition. Cerebral ischemia was induced by occluding the common carotid artery of experimental animals. The hippocampal areas were morphometrically analyzed at different time point after ischemic induction(1, 3, 5 days) by using immuno-histochemical technique and imaging analysis system. The number of parvalbumin-positive neurons in hippocampus was significantly reduced at 1 day after ischemia(p<0.05). Furthermore, the number of parvalbumin-immunoreactive neurons was dramatically reduced at 3 and 5 days after cerebral ischemic induction(p<0.05) as compared to 1 day group after ischemia, as well as sham control group. Significant reduction of parvalbumin positive neurons in CA1 region of hippocampus was observed at 1 day after cerebral ischemic induction. However, significant loss of MAP2 immunoreactivity was observed at 3 day after cerebral ischemia. The loss of parvalbumin-positive neurons and MAP2 immunoreactivity in CA1 region was prevented by pre-administration of GR89696 compared to that of saline-treated ischemic group. Furthermore, protective effect of GR89696 partially reversed by pre-treatment of naltrexone. These data indicate that parvalbumin-positive neurons more sensitively responded to cerebral ischemic damage than MAP2 protein. Moreover, this loss of parvalbumin-positive neurons was effectively prevented by the pretreatment of kappa opioid agonist. It was also suggested that the changes of number in parvalbumin-positive neurons could be used as the specific marker to analyze the degree of ischemic neuronal damage.

• Korean Journal of Food Science and Technology
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• v.26 no.6
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• pp.745-749
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• 1994

Animal experiments were conducted to confirm the suppressive effect of green tea on the intestinal absorption and tissue accumulation of toxic heavy metals in rats. When drinking water contaminated with 500 and 5000 times level of water quality standard for lead or cadmium was administrated to rats for 3 weeks, feed intake and body weight gain were not significantly differrent among all groups except for high cadmium group. In the relative weights of target organs, kidney and femur showed a significant difference by heavy metal administration and green tea did not influence on the weights. Green tea showed a suppressing effect on the accumulation of heavy metals in target organs, in which the reducing effect in femur was $25{\sim}45%$ for lead, and 42% for cadmium. As calcium content of femur decreased by heavy metal administration was increased in green tea group, it was concluded that heavy metal accumulation in femur was interrupted by tea beverage.

• Journal of Ginseng Research
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• v.41 no.3
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• pp.307-315
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• 2017

Background: Red-skin root disease has seriously decreased the quality and production of Panax ginseng (ginseng). Methods: To explore the disease's origin, comparative analysis was performed in different parts of the plant, particularly the epidermis, cortex, and/or fibrous roots of 5-yr-old healthy and diseased red-skin ginseng. The inorganic element composition, phenolic compound concentration, reactive oxidation system, antioxidant concentrations such as ascorbate and glutathione, activities of enzymes related to phenolic metabolism and oxidation, and antioxidative system particularly the ascorbate-glutathione cycle were examined using conventional methods. Results: Aluminum (Al), iron (Fe), magnesium, and phosphorus were increased, whereas manganese was unchanged and calcium was decreased in the epidermis and fibrous root of red-skin ginseng, which also contained higher levels of phenolic compounds, higher activities of the phenolic compound-synthesizing enzyme phenylalanine ammonia-lyase and the phenolic compound oxidation-related enzymes guaiacol peroxidase and polyphenoloxidase. As the substrate of guaiacol peroxidase, higher levels of $H_2O_2$ and correspondingly higher activities of superoxide dismutase and catalase were found in red-skin ginseng. Increased levels of ascorbate and glutathione; increased activities of $\text\tiny L$-galactose 1-dehydrogenase, ascorbate peroxidase, ascorbic acid oxidase, and glutathione reductase; and lower activities of dehydroascorbate reductase, monodehydroascorbate reductase, and glutathione peroxidase were found in red-skin ginseng. Glutathione-S-transferase activity remained constant. Conclusion: Hence, higher element accumulation, particularly Al and Fe, activated multiple enzymes related to accumulation of phenolic compounds and their oxidation. This might contribute to red-skin symptoms in ginseng. It is proposed that antioxidant and antioxidative enzymes, especially those involved in ascorbate-glutathione cycles, are activated to protect against phenolic compound oxidation.

• Journal of Oriental Physiology
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• v.14 no.2 s.20
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• pp.215-224
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• 1999

After Triton WR-1339 (TX; 600mg/kg) intraperitoneal injection, hepatic tissues of ICR mice were intragastric injected with Hyulboochucketang extract(HCE; 3.3ml/kg/day) were observed to investigate the suppressive effect of lipid accumulation that evoke by the antihyperlipidemic effect of HCE. These hepatic tissues were fixed in fromol-calcium solution and were cryocut. These tissues stained by H&E for general morphology, sudan black B for lipid and perchloric acid-naphthoquinone(PAN) method for cholesterol. After TX treatment, the increase of hepatocyte having meshlike cytoplasm(HHMC) were shown in all hepatic lobules and the hepatic plates were disappeared in the aggregative region of HHMC. The number of blue black colored lipid drop and dark green colored asterisk shaped cholesterol particle in hepatic cytoplasm were increased and the size of lipid drop and cholesterol particle were enlarged. But, in HCE-treated mice, the HHCM were disappeared and hapatic plate were rearranged. The number of lipid drop and cholesterol particle were decreased than TX-treated mice and the size of lipid drop and cholesterol particle were diminished. As results indicated that the HCE work on the suppression of lipid accumulation in hepatic tissue of hyperlipidemic mice caused by disturbance of lipid metabolism.

• Nutrition Research and Practice
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• v.17 no.1
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• pp.13-31
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• 2023

BACKGROUND/OBJECTIVES: Epigenetic regulation by nutrients can influence the development of specific diseases. This study sought to examine the effect of individual nutrients and nutrient families in the context of preventing chronic metabolic diseases via epigenetic regulation. The inhibition of lipid accumulation and inflammation by nutrients including proteins, lipids, vitamins, and minerals were observed, and histone acetylation by histone acetyltransferase (HAT) was measured. Correlative analyses were also performed. MATERIALS/METHODS: Nutrients were selected according to information from the Korean Ministry of Food and Drug Safety. Selected nutrient functionalities, including the attenuation of fatty acid-induced lipid accumulation and lipopolysaccharide-mediated acute inflammation were evaluated in mouse macrophage Raw264.7 and mouse hepatocyte AML-12 cells. Effects of the selected nutrients on in vitro HAT inhibition were also evaluated. RESULTS: Nitric oxide (NO) production correlated with HAT activity, which was regulated by the amino acids group, suggesting that amino acids potentially contribute to the attenuation of NO production via the inhibition of HAT activity. Unsaturated fatty acids tended to attenuate inflammation by inhibiting NO production, which may be attributable to the inhibition of in vitro HAT activity. In contrast to water-soluble vitamins, the lipid-soluble vitamins significantly decreased NO production. Water- and lipid-soluble vitamins both exhibited significant inhibitory activities against HAT. In addition, calcium and manganese significantly inhibited lipid accumulation, NO production, and HAT activity. CONCLUSIONS: Several candidate nutrients and their family members may have roles in the prevention of diseases, including hepatic steatosis and inflammation-related diseases (i.e., nonalcoholic steatohepatitis) via epigenetic regulation. Further studies are warranted to determine which specific amino acids, unsaturated fatty acids and lipid-soluble vitamins or specific minerals influence the development of steatosis and inflammatory-related diseases.

• The Korean Journal of Nuclear Medicine
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• v.38 no.5
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• pp.344-346
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• 2004

A 69-year-old woman was presented with progressed dysphagia, gastric soreness and weight loss during 2 months. She was performed abdomen x-ray, EGDS and abdomen CT. Abdomen x-ray demonstrated punctuate calcification on LUQ. EGDS showed an ulceroinfiltrative mass with bleeding on cardia to antrum of stomach. And CT showed diffuse gastric wall thickness with multiple calcifications. Biopsy of the stomach and esophagus during EGDS examination revealed an adenocarcinoma, with signet ring cell type, infiltrating the wall of the stomach and the distal esophagus. Then acne scan was performed a few days later. It revealed intense uptake in LUQ, corresponding to the calcium containing neoplasm seen on the abdomen x-ray, EGDS and abdomen CT. And there was no evidence of any metastatic lesion and thyroid uptake on the bone scan. There are many reports about accumulation of the tracer in extraosseous lesion, but only a few literatures were reported about gastric calcification in stomach cancer. More over, no reports showed CT images. We are performed many diagnostic examinations and found well correlation between them. The reason of gastric calcification is considered with calcium deposition within extracellular space due to hemorrhage or necrosis. Other possibility offered to explain gastric calcification have been increased blood flow and/or increased neovascularity with capillary leaks of tracer, and specific enzymatic (phosphatases) receptor binding of tracer. So, it was happened ion exchange between intracellular calcium and phosphate groups of tracer.

• Korean Journal of Soil Science and Fertilizer
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• v.43 no.4
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• pp.471-477
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• 2010

Although the roles of calcium in plant are widely known, little is known about on an antagonistic effect of macro elements, oxalate biosynthesis and main shape of crystal in cucumber plant organs. Seeds of cucumber (Cucumis sativus cv. Ijoeunbackdadagi) were germinated in perlite tray supplied with distilled-deionized water. Seedlings were transplanted into aerated containers with a half strength of Ross nutrient solution. Ca levels treated in media were as follows; No-Ca, $Ca(NO_3)_2$ 0.25, 1.25 and 2.5 mmol $L^{-1}$, and $Ca(NO_3)_2$ 2.5 mmol $L^{-1}$ + $CaCl_210$, 25 and 50 mmol $L^{-1}$. Ca-deficient and -excessive conditions severely reduced cucumber growth, as compared to the control, and adversely affected an accumulation of macro elements (N, P, K, and Mg). Calcium favorably induced oxalate (acid-soluble) synthesis in leaves and roots of cucumber plant, but not in stem. Acid-soluble oxalate contents in leaves proportionally increased with Ca supply levels (0.91, P<0.001), however, this pattern was not observed in stem and roots. Ca-oxalate crystal formation and compositional analysis were examined using SEM-EDS technique in cucumber leaves. The main type of crystal revealed a prismatic crystal and main components were Ca, Na and Cl.

• Journal of the Korean Society of Food Science and Nutrition
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• v.22 no.2
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• pp.109-115
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• 1993

This study was designed to investigate the effects of dietary protein and calcium levels on Ca, Fe, Cu, Zn and Mg accumulation of the tissue of the Pb-administered rats. Male Sprague-Dawley rats were assigned to a 3$\times$3$\times$2 factorial design with 3 levels of protein (40%, 15%, 6%), 3 levels of Ca (1.2%, 0.6%, 0.12%) and 2 feeding periods (3 and 7 weeks). The control group was included separately. The rats were exposed to the drinking water containing 2,000ppm of lead. Calcium contents in serum, liver and femur were decreased with Pb administration. Calcium contents in serum and femur were reduced with dietary protein and Ca levels. Level of serum Fe showed no significant difference with Pb administration in the rats fed the high Ca diet. Iron content in liver was not affected by the lead when the rats fed the diet containing high protein and Ca. Level of serum Cu was lower in the Pb added groups than in the control group and tended to be reduced with decreasing dietary protein and Ca levels. Copper content in liver was not affected by the lead when the rats fed the high Ca diet. Level of serum Zn was decreased in the low protein-low Ca group. Magnesium content in serum was decreased with Pb administration when the rats fed the diet containing low protein and Ca. However, magnesium content in liver was reduced with Pb administration and affected only by dietary protein level.

• Korean Journal of Agricultural Science
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• v.18 no.2
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• pp.104-113
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• 1991

This experiment was conducted to find the effect of calcium salts on the inhibition of 'Campbell Early' grape(Vitis labruscana B.) berry absicission induced by ethephon. Ethylene evolution, berry abscission rate, and fruit quality were examined. Several mineral contents were determinded in the berries and morphological changes of cells in abscission zone were also studied. 1. Calcium acetate($4.4-8.8g/{\ell}$) and calcium hydroxide($2.2-8.8g/{\ell}$) completely inhibited berry abscission when applied with ethephon 1,000 ppm while calcium chloride was less effective. 2. Ethylene evolution in the berries treated with ethephon alone reached to maximum peak one day after application. However, that peak in the treatment of ethephon with calcium acetate occurred 3 hours after application. 3. No decrease of ethylene production capacity was observed in the ethephon solution even when held up to 36 hours after preparation but the effect on berry abscission was significantly reduced. In contrast, ethylene production of the solution prepared with calcium acetate was greatly decreased along with the increase of holding time. The effect of calcium acetate in aged solution on the inhibition of berry abscission was not altered. 4. There were no differences in mineral contents such as Ca, Mg, and K between the berries sprayed with ethephon 500 ppm or not. Additional calcium accumulation was not found in the skin, flesh, and petiole of clusters sprayed with 3 kinds of calcium salts (1.5 g net Ca per liter) respectively when analyzed 2 weeks before maturity. 5. Ethephon (1,000 ppm) alone induced the development of abscission layer in petiole but those applied with calcium acetate (0.05 M) did not develop any distinct abscission layer 3days after treatment.