• The Journal of Dong Guk Oriental Medicine
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• v.6 no.1
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• pp.107-115
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• 1997

Hapatic tissues of ICR mouse intraperitoneally injected with Triton WR-1339 were observed to investigate the morphologic change of liver by destruction of lipid metabolism as hyperlipidemia. The hepatic tissues were obtained at hour-24, 48, and 72 after triton injection that were fixed in fromol-calcium solution and were cryocut. These tissues were stained by H&E for general morphology, sudan black B for lipid, and perchloric acid-naphthoquinone method for cholesterol. The increase of hepatocyte having meshlike cytoplasm were shown in all hepatic lobules after triton injection and the hepatic plates were disappeared in the region aggregated meshlike hepatocyte. The number of blue black colored lipid drop and dark green colored asterisk shaped cholesterol particle in hepatic cytoplasm were increased than the saline injected mouse and the size of lipid drop was enlarged. As results indicated that the lipid metabolism were destructed by triton injection, subsequently hepatocyes accumulated with lipid including cholesterol.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.19 no.2
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• pp.41-54
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• 2016

The production of high quality seedlings is a very important phase in silvicultural systems for successful reforestation or restoration. The purpose of this study was to measure both growth performances and nutrient responses of Fraxinus rhynchophylla and Pinus densiflora seedlings, which are commercially planted in Korea, according to soil improvement treatments. We applied 8 types of soil improvements: control with no treatment, compost B and compost Y as organic materials, vermiculite, perlite, two level of zeolite, and mix of vermiculite, perlite, and zeolite as inorganic materials in a permanent national nursery. Only compost B treatment significantly increased soil pH, organic matter, total nitrogen, available phosphorus, exchangeable potassium and calcium at the 0-10 cm soil depth. The growth of F. rhynchophylla and P. densiflora was the highest at the compost B treatment and the lowest at the vermiculate treatment. Compost B treatment allocated more carbon to aboveground than belowground by 39%, especially to foliage. On the vector diagnosis, there was 'shortage' on compost B treatment because of all increases of N contents, N concentrations, and growth and 'over accumulation' on vermiculite treatment because of more N uptake compared with dry weight increase. This study suggested optimal use of soil improvements is very important to improve soil quality in a permanently used nursery.

• Korean Journal of Environmental Biology
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• v.21 no.4
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• pp.400-404
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• 2003

This study was to evaluate the effects of gamma irradiation on the germination, nutrient concentrations and growth of spinach and radish. Both the spinach and radish seeds exhibited relatively higher germination rates in response to the low doses of gamma irradiation compared to the non -irradiated control. Leaf DW of the radish did not respond to gamma irradiation but that of the spinach increased significantly in response to a gamma radiation of 4 Gy (P< 0.05). Leaf growth parameters of the spinach including the leaf area and SLA (leaf area/leaf dry weight) also demonstrated increased responses to gamma irradiation. R/S (root dry weight/shoot dry weight), root DW and root length of the spinach exhibited a positive response to gamma irradiation while those of the radish did not. In contrast, SRL (root length/root dry weight) significantly decreased with gamma irradiation at 8 Gy for the spinach, but not for the radish. The tissue nitrogen concentrations of the spinach showed an increased response to gamma irradiation while that of the radish did not. Furthermore, higher concentrations of phosphorus, potassium, calcium and magnesium were found in the irradiated spinach, but not in the irradiated radish. It seems that the non-specific physiological and/or biochemical activities of spinach might be accelerated by gamma irradiation, possibly accounting for the stimulation of nutrient uptake from the root media and early biomass accumulation in the current study.

• The Korean Journal of Pharmacology
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• v.31 no.3
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• pp.333-344
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• 1995

The effects of adenosine and $N^6-cyclopentyladenosine$ (CPA) on superoxide production, myeloperoxidase release and $Ca^{2+}$ mobilization stimulated by fMLP in neutrophils were investigated. The effects were also observed on the stimulatory actions of C5a and PMA and the responses in lipopolysaccharide-primed neutrophils. In addition, the involvement of cAMP in the inhibitory action of adenosine was examined. The fMLP-stimulated neutrophil respiratory burst, degranulation and intracellular $Ca^{2+}$ mobilization may be regulated by activation of adenosine receptors. Adenosine may not affect the stimulated neutrophil responses due to activation of protein kinase C. fMLP-stimulated respiratory burst in lipopolysaccharide-primed neutrophils may be less sensitive to adenosine, compared with nonprimed cells. The inhibitory effect of theophylline in the presence of adenosine on neutrophil responses appears to be ascribed to accumulation of intracellular cAMP.

• Journal of Nutrition and Health
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• v.31 no.3
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• pp.315-323
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• 1998

This study was performed to investigate the serum lipid levels and fatty acid composition in middle-aged men and the influence of cardiovscular risk factors on them. Data for dietary intake, anthropometry , blood pressure, and general environmental factors were obtained and their relationship to serum lipid levels and fatty acid composition were analyzed in 303 healthy male subjects with a mean age of 43.8$\pm$6.4 years from June 1 to 22 , 1995. The prevalence of obesity was 26.7% by BMI and 11.2% by WHR criteria . The percentage of hypertensive subjects were 13.5% and 22.4% by systolic and diastolic blood pressure, respectively. Retinol, calcium , and roboflavin intakes were lower than the RDA for Koreans. The ratio of carbohydrate intake to total calories of the subjects was 70%. Ratios of P/M and n-6/n-3 were 1.02/1.05/1.00 and 11.1/1, respectively. The percentage of subjects who had serum TG, TC, LDL-C leves beyond the normal range were 19.5% , 13.5% and 8.6% respectively. Palmitic comprised 26.4% of serum total fatty acids, linoleic acid 19.4% and oleic acid 16.4. The waist circumference and ration of carbohydrate intake were related positively to TG level. Serum cholesterol level had positive relations with waist circumference, DBP, sugar intake, and income. There were positive relationships between SFA and bone fishes and vegetable oil intakes, MUFA and DBP while a negative a negative relationship was observed between PUFA and animal fat intake. Serum n-6 fatty acid was related negatively to carbohydrate intake and hot taste preference. N-3 fatty acid was related positively to fish intake and negatively to egg intake. These results suggest that to maintain adequate serum lipid levels and fatty acid composition, sugar, animal fat, and carbohydrate intakes have to be reduced, while consumption of vegetable oil and fish must be increased. It is also essential to control abdominal fat accumulation and blood pressure.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.50 no.3
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• pp.270-277
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• 2017

This study was carried out to investigate seasonal changes in body composition in Boleophthalmus pectinirostris in Korea. B. pectinirostris collected in Gangjin, Jeonnam, consisted of 58.7-75.8% moisture content, 16.1-17.6% protein, 0.8-3.4% crude lipids, and 1.3-3.6% crude ash. Livers consisted of 15-17% moisture content, 14.0-16.8% crude protein, 34.4-71.2% crude lipids, and 0.5-1.2% crude ash. Calcium, iron, potassium, etc., content in B. pectinirostris muscle averaged 267.5-599.8 mg/100 g, 1.98-28.3 mg, 160.7-327.9 mg, 20.6-60.2 mg, 0.4-2.2 mg, 0.23-0.46 mg, 66.5-192.9 mg, and 1.32-3.8 mg, respectively. (Please clarify: the list of measured values must correspond directly with what was measured, not just "etc.") The major amino acids in B. pectinirostris muscle, in proportional order, were glutamic acid, isoleucine, and leucine; whereas the major amino acids in the liver were glutamic acid, lysine, aspartic acid, and leucine. The major saturated fatty acids in the muscle were palmitic acid (15-19%) and stearic acid (8-11%). The major monoenes and polyenes were palmitoleic acid (C16:1) and oleic acid (C18:1), and EPA and DHA, respectively. The major saturated fatty acids and polyenes in the liver were the same as in muscle, but the monoenes were palmitoleic acid (C16:1) and cis-10-hepta-decenoic acid (C17:1). Seasonal changes in B. pectinirostris body composition may be attributable to differential accumulation of fat and nutrients in comparison to the spawning and hibernation period.

• Korean Journal of Soil Science and Fertilizer
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• v.49 no.6
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• pp.635-643
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• 2016

Wine consumers desire to drink a high quality wine. For producing high quality wine, high quality soil is required. Conventionally, soil quality is assessed qualitatively. Using traditional laboratory methods, quantitative data can be obtained for management purpose, but it is time consuming and expensive. Therefore, new technology aims to address these limitations, namely portable X-Ray fluorescence spectrometers (pXRF). This instrument can be used directly in the field, requires no soil sample preparations, and can simultaneously measure a wide range of elements qualitatively that are useful for pedological studies. The chemical composition (Ca, Fe, Ti and Zr) of soils at Tallavera Grove vineyard in New South Wales, Australia, was studied using a pXRF. The analysis of the soil's elemental concentration (i.e. Ca and Fe) using pXRF supports management decisions. Measuring the soil's Ca concentration can be used to identify Ca-rich parent materials (limestone). The limestone indicates good soil conditions for vine production. Fe content was used to identify areas of texture-contrast soils or soil with accumulation of clays in the B horizon. In addition, a soil weathering index was calculated using elemental concentrations (i.e. Ti and Zr) to explore the history of soil formation for making decision of management. This index showed that the soil in the vineyard was affected by two processes: the deposition of materials from elsewhere (Aeolian transport or soil erosion) and mixing of materials from upslope.

• BMB Reports
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• v.48 no.11
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• pp.636-641
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• 2015

There are controversial findings regarding the roles of nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway on bone metabolism under oxidative stress. We investigated how Nrf2/HO-1 pathway affects osteoblast differentiation of MC3T3-E1 cells in response to hydrogen peroxide (H₂O₂), N-acetyl cysteine (NAC), or both. Exposing the cells to H₂O₂ decreased the alkaline phosphatase activity, calcium accumulation, and expression of osteoblast markers, such as osteocalcin and runt-related transcription factor-2. In contrast, H₂O₂ treatment increased the expression of Nrf2 and HO-1 in the cells. Treatment with hemin, a chemical HO-1 inducer, mimicked the inhibitory effect of H₂O₂ on osteoblast differentiation by increasing the HO-1 expression and decreasing the osteogenic marker genes. Pretreatment with NAC restored all changes induced by H₂O₂ to near normal levels in the cells. Collectively, our findings suggest that H₂O₂-mediated activation of Nrf2/HO-1 pathway negatively regulates the osteoblast differentiation, which is inhibited by NAC.

• Toxicological Research
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• v.21 no.4
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• pp.291-295
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• 2005

To define the effect of silica on the stimulator of signaling pathway, we studied the phospholipase D (PLD) activity in the Rat2 fibroblasts. Silica stimulated the accumulation of labeled $[^3H]$ phosphatidylethanol$([^3H]\;PEt)$ in a time- and concentration-dependent manner. This Silicainduced PLD activity was partially attenuated by the pretreatment with U73122 (phospholipase C inhibitor), genistein (protein tyrosine kinase inhibitor), PD 98056 (MEK inhibitor) and mepacrine (phospholipase $A_2$ inhibitor). But, sphingosine (protein kinase C inhibitor) and DPI (NADPH reductase inhibitor) had not effect the PLD activity. Silica also increased the PLD activity about four fold, which imply that the PLD activity is more influenced by the mobilization of PLD than other signaling mediators. The PLD activity also partially inhibited calcium chelator EGTA or/and BAPTA/AM compared to silica. Finally, we concluded that a silica-stimulated phospholipase D activity is present in the Rat2 fibroblasts and is modulated by combination of various signaling mediators.

• Pediatric Gastroenterology, Hepatology & Nutrition
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• v.14 no.1
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• pp.86-90
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• 2011

Wilson's disease is an autosomal recessive disorder of copper metabolism consequence of which leads to accumulation of copper in the liver, brain, cornea and other tissues. The manifestations are more likely to be hepatic in the early childhood and neurological in the adolescents. In addition, the abnormalities that develop during disease progression may result in other manifestations such as hematologic, endocrine, or renal findings. We report a thirteen year-old girl who manifested tetany shortly after the initial diagnosis of Wilson's disease. Despite aggressive calcium, magnesium and vitamin D replacement, the hypocalcemia and hypomagnesemia did not respond to the therapy promptly. It took more than three weeks for blood levels of the minerals to be normal. We concluded that tetany occurred in our patient because of hypoparathyroidism as a rare complication of Wilson disease, vitamin D deficiency resulting from various conditions, and inconclusive hypomagnesemia.