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http://dx.doi.org/10.5483/BMBRep.2015.48.11.112

N-acetyl cysteine inhibits H2O2-mediated reduction in the mineralization of MC3T3-E1 cells by down-regulating Nrf2/HO-1 pathway  

Lee, Daewoo (Institute of Oral Biosciences and School of Dentistry)
Kook, Sung-Ho (Institute of Oral Biosciences and School of DentistryDepartment of Bioactive Material Sciences and Institute of Molecular Biology and Genetics, Chonbuk National University)
Ji, Hyeok (Institute of Oral Biosciences and School of Dentistry)
Lee, Seung-Ah (Department of Nursing, Chonnam Techno College)
Choi, Ki-Choon (Grassland and Forage Division, National Institute of Animal Science, RDA)
Lee, Kyung-Yeol (Institute of Oral Biosciences and School of Dentistry)
Lee, Jeong-Chae (Institute of Oral Biosciences and School of DentistryDepartment of Bioactive Material Sciences and Institute of Molecular Biology and Genetics, Chonbuk National University)
Publication Information
BMB Reports / v.48, no.11, 2015 , pp. 636-641 More about this Journal
Abstract
There are controversial findings regarding the roles of nuclear factor (erythroid-derived 2)-like 2 (Nrf2)/heme oxygenase-1 (HO-1) pathway on bone metabolism under oxidative stress. We investigated how Nrf2/HO-1 pathway affects osteoblast differentiation of MC3T3-E1 cells in response to hydrogen peroxide (H2O2), N-acetyl cysteine (NAC), or both. Exposing the cells to H2O2 decreased the alkaline phosphatase activity, calcium accumulation, and expression of osteoblast markers, such as osteocalcin and runt-related transcription factor-2. In contrast, H2O2 treatment increased the expression of Nrf2 and HO-1 in the cells. Treatment with hemin, a chemical HO-1 inducer, mimicked the inhibitory effect of H2O2 on osteoblast differentiation by increasing the HO-1 expression and decreasing the osteogenic marker genes. Pretreatment with NAC restored all changes induced by H2O2 to near normal levels in the cells. Collectively, our findings suggest that H2O2-mediated activation of Nrf2/HO-1 pathway negatively regulates the osteoblast differentiation, which is inhibited by NAC.
Keywords
H2O2; N-acetyl cysteine; Nrf2; Osteoblast differentiation; Oxidative stress;
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Times Cited By KSCI : 4  (Citation Analysis)
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