• Korean Journal of Soil Science and Fertilizer
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• v.17 no.3
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• pp.253-257
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• 1984

A field experiment was conducted in order to secure the basic information on the rational application of nitrogen and potassium for autumn growing Chinese cabbage (Brassica campastris ssp. pekinensis, var.; Miho 70 days). The results of the experiment are summarized as follows: 1. Potassium effect was observed where nitrogen applied either less than 15kg N/10a and heavy dose of 25kg N/10a. No potassium effect was observed where 20kg N/10a applied. In the case of 25kg N/10a, potassium effect was observed only in total weight but failed yield marketable product weighing over 1 kilogram per cabbage. 2. Nitrogen application, in general, tends to lower the soil pH and it is particularly true when heavy dose of over 20kg N/10a is applied. As a result, Chinese cabbage has increasingly removed soil born potassium and reduced exchangeable potassium content of the soil. 3. Oven dried cabbage which received 25kg N/10a plus potassium showed a low concentration of calcium and this phenomena seems to be attributable to the acidification of soils and it further caused failure in producing cabbages of marketing value.

• The Plant Pathology Journal
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• v.18 no.4
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• pp.192-198
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• 2002

Mixed infections of two economically important viruses, Turnip mosaic virus(TuMV) in the family Potyviridae and Ribgrass mosaic virus(RMV) in the genus Tobamo-virus, were studied ultrastructurally on oriental cabbage. TuMV-ACl8 (alpine isolate in Korea) induced chlorotic spots on inoculated leaves of both ‘SSD63’ inbred line known as susceptible to TuMV, and ‘Tambok’ commercial cultivar, known as resistant to the virus, in the early stages of infection. TuMV-C5 (Taiwan isolate) caused severe mosaic and malformation on the upper leaves of ‘SSD63’, and necrotic spots in both inoculated and upper leaves of ‘Tambok’. RMV-CA1 (oriental cabbage isolate from alpine in Korea) induced vein chlorosis, leaf malformation, and midrib necrotic streak in the upper leaves of both ‘SSD63’ and ‘Tambok’. Both oriental cabbages infected with a combination of TuMV-ACl8 and RMV-CA1 showed synergistic symptoms of severe yellowing, severe mosaic, and necrotic spot or vein necrosis on their leaves. A combination of TuMV-C5 and RMV-CA1 produced synergistic symptoms only in ‘SSD63’. In ‘Tambok’ infected with the combination of TuMV-C5 and RMV-CA1, the number of necrotic spots on the inoculated leaves was one half lesser than that on singly infected with TuMV-C5. A few necrotic spots progressed systemically. In cells infected with a combination of TuMV-ACl8 and RMV-CA1 or TuMV-C5 and RMV-CA1, the particles of the two viruses made nonagon-like rings(NLR); one TuMV particle was surrounded loosely by nine RMV particles. Two unrelated viruses of TuMV and RMV were compacted in the central part of the spiral aggregates(SA) that was induced strikingly in cells by the mixed infections. The SA showed NLR in its center of the cross-sectioned side. Many particles of RMV of Tobamovirus were closely associated with Potyvirus-characteristic cylindrical inclusions. The SAs in the mixed infections were formed easily by the Potyvirus of TuMV-ACl8 or -C5 isolates.

• Journal of the Korean Society of Food Science and Nutrition
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• v.40 no.12
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• pp.1743-1749
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• 2011

The microbial reduction and quality characteristics of salted Chinese cabbage using electrolyzed water were investigated. The electrolyzed water was used to control the microbes in the processes of primary washing, salting, and secondary washing. The total bacteria, lactic acid bacteria, coliform, pH, salinity, vitamin C, and total sugar were analyzed. After primary washing by electrolyzed water, the total bacteria populations were reduced to 2.78 log cfu/g, and the coliform populations were similarly reduced. After secondary washing by electrolyzed water, the total bacteria population of Chinese cabbage was reduced to a maximum of 1.5 log cfu/g. The salinity of Chinese cabbage and salting solutions increased rapidly over three hours, and then increased slowly. The sterilization effect of electrolyzed salting water could not last beyond 3 hours, because the OHCl concentration of electrolyzed water was reduced by over 90% at the third hours of the salting process. Vitamin C was reduced and total sugar did not change regardless of treatments during the salting process. Consequently, electrolyzed water was effective to remove microbes from salted Chinese cabbages.

• Korean journal of applied entomology
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• v.47 no.4
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• pp.401-405
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• 2008

This study was conducted to estimate the control thresholds (CTs) of imported cabbage worm, Artogeia rapae L., injuring Chinese cabbage. The second instar larvae of A. rapae were inoculated with five density levels on each Chinese cabbages transplanted three weeks earlier under greenhouse condition, and checked injury rates after allowing their feeding for one week and two weeks, respectively. The average leaf area consumed by single larvae was 657.7 $mm^2$ in plots inoculated at three weeks after transplanting (WAT) and 2495.8 $mm^2$ in plots at 6-WAT, respectively. In the field experiment, different numbers of A. rapae ranged from one to seven larvae were inoculated on 20 plants. The percent yield reduction (Y) of Chinese cabbage infested by different densities of A. rapae (X) for a three-week period was estimated by the following equation; (1) Y=1.764X-0.3049 ($R^2$=0.9901) in plots inoculated at 3-WAT; and (2) Y=1.0305X-0.2976 ($R^2$=0.9398) in plots inoculated at 6-WAT. Based on the relationships between the densities of A. rapae larvae and the yield index of Chinese cabbage, the number of second instar larvae which caused 5% loss of yield (gain threshold proposed by Japan), was estimated as 3.0 per 20 plants for the 3-WAT and 5.1 for the 6-WAT.

• Horticultural Science & Technology
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• v.33 no.2
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• pp.242-250
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• 2015

Insertional mutagenesis induced by T-DNA or transposon tagging offers possibilities for analysis of gene function. However, its potential remains limited unless good methods for detecting the target locus are developed. We describe a PCR technique for efficient identification of DNA sequences adjacent to the inserted T-DNA in a higher plant, Chinese cabbage (Brassica rapa ssp. pekinensis). This strategy, which we named variable argument thermal asymmetric interlaced PCR (VA-TAIL PCR), was designed by modifying a single-step annealing-extension PCR by including a touch-up PCR protocol and using long gene-specific primers. Amplification efficiency of this PCR program was significantly increased by employing an autosegment extension method and linked sequence strategy in nested long gene-specific primers. For this technique, arbitrary degenerate (AD) primers specific to B. rapa were designed by analyzing the Integr8 proteome database. These primers showed higher accuracy and utility in the identification of flanking DNA sequences from individual transgenic Chinese cabbages in a large T-DNA inserted population. The VA-TAIL PCR method described in this study allows the identification of DNA regions flanking known DNA fragments. This method has potential biotechnological applications, being highly suitable for identification of target genomic loci in insertional mutagenesis screens.

• Korean Journal of Food Science and Technology
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• v.29 no.2
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• pp.292-301
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• 1997

In drying process, to minimize the quality degradation by improved drying process and pretreatment methods, carrots, cabbages and radishes were dried and rehydrated. Physico-chemical properties of product were analyzed to determine the optimum pretreatment method and drying models were applied to explain drying mechanisms. Microwave, steam and water were used prior to drying as blanching method. In consideration of physical properties, optimum treatment time was decided that microwave was 1 min, steam and water were each 10 min. Control, steam, water, microwave and osmotic dehydration were treated prior to drying as pretreatment individually, osmotic dehydration was lower than the other treatmemt in drying efficiency, but carotene content was higher than the others. The effect continued after rehydration. Ten panelists tested dried and rehydrated carrots. After rehydration, the quality of air dried product with osmotic dehydration was superior to freeze dried without treatment. The fittness of drying models were conducted in order to explain the mechanism of drying each process. Quadratic model was most fittable to explain during drying. However, in rehydration process, no fittable model was found.

• Korean Journal of Environmental Agriculture
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• v.14 no.3
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• pp.312-318
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• 1995

Residues of benfuresate in rice and oxolinic acid in Chinese cabbage were determined through the field experiments in order to establish the safe use and the maximum residue limit(MRL) of these pesticides in Korea. The herbicide benfuresate powder was sprayed into the paddy field with a level of 0.6kg(active ingredient)/ha and rice (Oryza sativa L.) was grown. At harvest, residues of benfuresate in brown rice and stem were analyzed using gas chromatograph. The residue of oxolinic acid in Chinese cabbage (Brassica campestris subsp. napus var pekinesis MAKINO) was analyzed using HPLC after foliar-spraying this fungicide into the cabbages at a level of 15kg/ha. The recovery efficiencies of benfuresate and oxolinic acid were 87-89% and 90-95%, respectively. The respective residues of benfuresate in rice and oxolinic acid in Chinese cabbage were in the range of 0.27-0.46 mg/kg and 0.23-1.53kg/kg. Residual concentrations of these pesticides in crops increased with the increased application frequencies, followed the first-order kinetics and linearly decreased with time. The highest residue of 1.53 mg/kg of oxolinic acid was observed when this fungicide was sprayed six times until three days prior to harvest, but this level was far lower t㏊n 5 mg/kg, which is the maximum residue limit(MRL) set by FAO/WHO.

• Korean Journal of Soil Science and Fertilizer
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• v.23 no.3
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• pp.173-179
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• 1990

The effect of a highly water absorbing polyacrylate polymer, commonly called K-sorb, at rates of 0.0, 0.05, 0.2, and 0.5% by weight on the water retention properties of three soils, and the longevities of these treatment effects were evaluated. Water retentions were measured for all the treatments by use of a pressure-plate extractor in the laboratory. Available water and three-phase distributions at moisture tensions of 0.01, 0.3, and 15b were calculated from water retentivity data. A randomized block experiment of Chinese cabbages was conducted to examine the effects and the longevities of the treatments(0.0, 0.05, 0.1, and 0.2%) on water retention of Jungdong sandy loam soil in the field. Water retentions for a loamy sand, sandy loam, and loam soil, treated with 0.2 and 0.5% K-sorb, were increased. K-sorb treatments were more effective in sandy soil than in loamy soils. Water contents for the 0.5% treatment were markedly greater than those for the 0.2% treatment at earth moisture tension. K-sorb only at a rate of 0.5% remained effective in water retention of each soil through repeated drying and wetting for 12 months. Duncan's multiple range showed 0.2% treatment was effective(at the level of 0.05) after 2 months but not after 10 months under field condition.

• Journal of Food Hygiene and Safety
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• v.22 no.4
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• pp.288-293
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• 2007

To evaluate effects of airborne microorganisms in fresh cut processing plant, microorganisms in air, equipments, raw material, water and final product were isolated and identified using Vitek (R)2 compact system. Airborne microorganisms were isolated from 1000L air using air sampler for floating microorganisms and plate count agar for falling microorganisms. And contaminated microorganisms of equipment, water, and product were isolated from plate count agar plate. Total plate counts for floating and falling, raw material, equipments and final product were $10^2-10^3CFU/m^3,{\sim}10^1CFU/plate,\;10^3CFU/g,{\sim}10^4CFU/cm^2\;and\;10^4CFU/g$, respectively. From the result of isolated microorganism identification from raw material to final product, airborne microorganisms could affect the flora of final product.

• Analytical Science and Technology
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• v.26 no.1
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• pp.67-72
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• 2013

This study has been conducted to provide a management plan for recycled products (plastic wastes) by identifying hazardous substances present in it and conducting exposure assessment. The concentration of 7 heavy metals (Cd, Cr, Cu, Fe, Hg, Pb, Zn) was analyzed. Exposure scenario was assumed by the consumption of kimchi made in a recycled plastic bucket. According to the analysis, all of the analyzed samples were within the food code standard level except one sample (137.03 mg/kg, sum of Cd, $Cr^{6+}$, Pb and Hg). In leaching test, all of heavy metals were within the leaching standard (Standards specifications of utensil and container-Packing). In an oral exposure test, Fe, Pb and Zn were detected in cabbages and were below PMTDI (Provisional Maximum Tolerable Daily Intake). As one of the recycled plastic buckets exceeded the standard level, we conclude not to recommend the recycled plastic buckets for cooking purpose.