• Title/Summary/Keyword: cRPN

Search Result 13, Processing Time 0.016 seconds

Identification of Proteins Affected by Iron in Saccharomyces cerevisiae Using Proteome Analysis

  • Lieu Hae-Youn;Song Hyung-Seok;Yang Seung-Nam;Kim Jae-Hwan;Kim Hyun-Joong;Park Young-Doo;Park Cheon-Seok;Kim Hae-Yeong
    • Journal of Microbiology and Biotechnology
    • /
    • v.16 no.6
    • /
    • pp.946-951
    • /
    • 2006
  • To study the effect of iron on Saccharomyces cerevisiae, whole-cell proteins of Saccharomyces cerevisiae were extracted and subjected to two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), and differentially expressed proteins were identified. The proteins separated were further identified by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry and were compared with a protein database. Of more than 300 spots separated by molecular weight and isoelectric points, 27 differentially expressed spots were identified. Ten proteins were found to be differentially expressed at high iron concentration. Triosephosphate isomerase (TPI), YDR533C hypothetical protein, superoxide dismutase (SOD), 60 kDa heat-shock protein (HSP60), pyruvate dehydrogenase beta subunit 1 (PDB1), and old yellow enzyme 2 (OYE2) were upregulated, whereas thiol-specific antioxidant (TSA), regulatory particle non-ATPase subunit 8 (RPN8), thiol-specific peroxiredoxin 1 (AHP1), and fructose-1, 6-bisphosphate adolase (FBA) were downregulated by iron. Based on the result, we propose that SOD upregulated by iron would protect the yeast from oxidative stress by iron, and that TSA downregulated by iron would render cells hypersensitive to oxidative stress.

Thermal Characteristic and Failure Modes and Effects Analysis for Components of Photovoltaic PCS (태양광 발전 PCS 구성부품에 대한 열적특성 및 고장모드영향분석)

  • Kim, Doo-Hyun;Kim, Sung-Chul;Kim, Yoon-Bok
    • Journal of the Korean Society of Safety
    • /
    • v.33 no.4
    • /
    • pp.1-7
    • /
    • 2018
  • This paper is analyzed for the thermal characteristics(1 year) of the 6 components(DC breaker, DC filter(including capacitor and discharge resistance), IGBT(Insulated gate bipolar mode transistor), AC filter, AC breaker, etc.) of a photovoltaic power generation-based PCS(Power conditioning system) below 20 kW. Among the modules, the discharge resistance included in the DC filter indicated the highest heat at $125^{\circ}C$, and such heat resulting from the discharge resistance had an influence on the IGBT installed on the rear side the board. Therefore, risk priority through risk priority number(RPN) of FMEA(Failure modes and effects analysis) sheet is conducted for classification into top 10 %. According to thermal characteristics and FMEA, it is necessary to pay attention to not only the in-house defects found in the IGBT, but also the conductive heat caused by the discharge resistance. Since it is possible that animal, dust and others can be accumulated within the PCS, it is possible that the heat resulting from the discharge resistance may cause fire. Accordingly, there are two options that can be used: installing a heat sink while designing the discharge resistance, and designing the discharge resistance in a structure capable of avoiding heat conduction through setting a separation distance between discharge resistance and IGBT. This data can be used as the data for conducting a comparative analysis of abnormal signals in the process of developing a safety device for solar electricity-based photovoltaic power generation systems, as the data for examining the fire accidents caused by each module, and as the field data for setting component management priorities.

The Growth of $MgO:LiNbO_3$ Single Crystal by Czochralski Method and its Density Measurement (Czochralski법에 의한 $MgO:LiNbO_3$단결정 성장과 밀도 측정)

  • Kim, Il-Won;Park, Bong-Chan;Kim, Gap-Jin
    • Korean Journal of Crystallography
    • /
    • v.4 no.2
    • /
    • pp.74-85
    • /
    • 1993
  • Single crystals of LiNbO3 have found extensive application in electro-optic and nonlinear optic devices. However, laser-induced refartive index inhomogeneities, which have been labeled opical damage impose limits on device optical damage in LiNbO3 is imporved if more than 4.5 rml% MgO is added to the melt The laser damage thrueshold increased as much as 100 times better then that of undoped crystals. The MgO doped cystal has thus been urterlsiv81y studied since then. In the study, Mgo:LiNbOs(MLA) single crystals dopsd with 0, 2.5, 5.0, 7.5, 10.0 mol% MgO have been grown by the czocrualski technique. The metls were prepared in the platinum crluible and 15∼20mm diameter crystals were grown with a length of 20∼30mm in a resitance heater. The growth rate was 2.5mm/hr, the rotation speed 15rpn. Before sawing MLN single crystals were annealed for 24 hours under atmosphere at a temperature of 1080℃. After sawing, we have found an annual ring cross section of MNA crystals only in the direction of perpendicilar to the c-axis. Nonuniform dispusion of MgO was pointed out that the cuties of the state of oxide were strongly affected by oxygen partial pressure in.

  • PDF