• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.643-649
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• 2010

Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine the etiologic agent, CSF and/or stool specimens from 140 children hospitalized for aseptic meningitis at Soonchunhyang University Cheonan Hospital between June and October of 2008 were tested for virus isolation and identification. E30 accounted for 61.7% (37 cases) and echovirus 6 accounted for 21.7% (13 cases) of all the human enteroviruses (HEVs) isolates (60 cases in total). For the molecular characterization of the isolates, the VP1 gene sequence of 18 Korean E30 isolates was compared pairwise using the MegAlign with 34 reference strains from the GenBank database. The pairwise comparison of the nucleotide sequences of the VP1 genes demonstrated that the sequences of the Korean strains differed from those of lineage groups A, B, C, D, E, F, and G. Reconstruction of the phylogenetic tree based on the complete VP1 nucleotide sequences resulted in a monophyletic tree, with eight clustered lineage groups. All Korean isolates were segregated from other lineage groups, thus suggesting that the Korean strains were a distinct lineage of E30, and a probable cause of this outbreak. This manuscript is the first report, to the best of our knowledge, of the molecular characteristics of E30 strains associated with an aseptic meningitis outbreak in Korea, and their respective phylogenetic relationships.

• Korean Journal of Animal Reproduction
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• v.26 no.4
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• pp.377-384
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• 2002

To investigate the expression patterns of proteins and growth factor signals in differentiated rabbit embryonic stem (ES) cells, ES cells with confluent stage grown of feeder layer and differentiated cells into embryoid bodies (EB) without feeder cell were applied to protein gel and Western blotting analysis. There were 66kDa and 28kDa specifically expressed in differentiated ES cell but not in undifferentiated ES cell while 25kDa protein band showed up in only undifferentiated ES cells. Also there were some difference of protein bands in several area of gel between differentiated and undifferentiated ES cells such as about 100 kDa, 50kDa and 27kDa areas, but there was no difference in band pattern of one-dimensional gel analysis between mouse ES cells and rabbit ES cells. IGF-I receptor and EGF receptor were expressed in differentiated cells and undifferentiated cells. And ICF-I and EGF were not expressed in both differentiated and undifferentiated cells. These results indicated that ES cells express their own proteins to inhibit differentiation while EB cells synthesize different proteins to differentiate, and 16F-I receptor and EGF receptor were expressed in both ES and EB cells probably for the different functions.

• Journal of Animal Science and Technology
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• v.47 no.2
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• pp.147-158
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• 2005

The purpose of this study was to find out the effects that daughters' incomplete lactation records affect sire's breeding values through genetic evaluation using RRTDM(random regression test-day model). First, we estimated genetic parameters and breeding values on sires having complete lactation records of daughter by RRTDM, second, we changed complete lactation records of specific sires into incomplete records by various methods. Third, the breeding values were compared between complete and incomplete records. Finally, this study aimed to find out the methods to minimize the estimation errors of young bulls' breeding values. Data used in this study were collected from the dairy herd improvement program, and a total of 97,562 records were composed of 10,929 first parity with both parents known, since 1999. Breeding values on the daughters from randomly chosen sires were calculated and compared with among 90 day, 150day, and 200 day's incomplete records. For milk yields, sire's ranks of breeding values used by complete lactation records were very different from sire's ranks of breeding values obtained by incomplete lactation records(Rank_90 cut, 150cut, 200 cut).The differences were also obtained between complete lactation records(per305_full) and incomplete lactation record (per_90 cut, 150cut, 200 cut) in breeding values regarding persistency. Especially, the differences between per_90 cut and per305_full were very large(from 1.8 kg to 145kg).

• Microbiology and Biotechnology Letters
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• v.24 no.2
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• pp.234-241
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• 1996

Two hundreds and thirty lactic acid bacteria, mostly isolated from Kimchi, including type strains were used for analysis of cellular fatty acids. The 230 test strains were recoverd in 7 major and 1 single clusters defined at Euclidian distance of 17.5. These aggregate taxa were equivalent to the genus Leuconostoc (aggregate group A, B, C and D), the genus Lactobacillus (aggregate group F), the genera Lactobacillus and Pediococcus (aggregate group E) and the genera Leuconostoc and Lactobacillus (aggregate group G). It is concluded as evident that FAMEs (Fatty Acid Methyl Esters) profile of cell can be used as a criterion in classification of lactic acid bacteria from kimchi. Additional comparative taxonomic studies need to be carried out on well chosen representative strains to determine the most appropriate methods of value.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 1996.06c
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• pp.263-269
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• 1996

In indian agricultural , the energy use pattern has played dominant role in influencing the level of mechanization . Besides that the agro-climatic factors as well as the size of holdings do affect the degree of mechanization . Nearly 30 percent of total cultivated area is owned by l76 percent of the small and tiny land holders each owning even less than a hectare. On the other hand, about 2 percent of land owners cultivate land. These variabilitieshave greatly influenced the ownership of power sources on Indian farms. Small farmers, employ human and animal energies with the use of hand tools and animal drawn equipments. Whereases, the use of tractors, power tillers, electric motors, etc. on small farms is on a marginal scale. There are few pockets and also extensive wheat growing regions where mechanical and electrical power sources are extensively used in production agriculture leading to about 185% of cropping intensity . In that region, the animal energy is employed for on the farm transport of fertilizers, fodders and fuel to support milch animals and other household activities . Inspite of high degree of mechanization, the harvesting of crops is done by human labour with few exceptions of harvesting wheat crops by combines in few pockets. In overall assessment of mechanization, the following conclusions are drawn : ⅰ) Farm operation which show a growing trend of mechanization are (a) tillge, (b) seedling (c) Irrigation (d) Plant protection application (e) Threshing and (f) Transport . ⅱ) Crop cultivation system in respect of wheat, maize and sorghum have been greatly mechanized. ⅲ) The least mechanized cropping systems are (a) vegetable production and (b) cultivation of sugarcane, cotton, rice and pulses. ⅳ) Annual production of tractor has touched the figure of 280.000 by 1995 and the total number has crossed 1.5million on Indian farms.

• Journal of Soil and Groundwater Environment
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• v.16 no.6
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• pp.133-142
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• 2011

A total of 247 samples were collected from groundwater being used for drinking-water supply, and hydrogeochemistry and radionuclide analysis were performed. In-situ analysis of groundwaters resulted in ranges of $13.7{\sim}25.1^{\circ}C$ for temperature, 5.9~8.5 for pH, 33~591 mV for Eh, $66{\sim}820{\mu}S/cm$ for EC, and 0.2~9.4 mg/L for DO. Major cation and anion concentrations of groundwaters were in ranges of 0.5~227.6 for Na, 1.0~279.3 for Ca, 0.0~9.3 for K, 0.1~100.1 for Mg, 0.0~3.3 for F, 0.9~779.1 for Cl, 0.3~120.4 for $SO_4$, 0.0~27.4 for $NO_3$-N, and 6~372 mg/L for $HCO_3$. Uranium-238 and radon-222 concentrations were detected in ranges of N.D-$131.1{\mu}g/L$ and 18-15,953 pCi/L, respectively. In case of some groundwaters exceeding USEPA MCL level ($30{\mu}g/L$) for uranium concentration, their pH ranged from 6.8 to 8.0 and Eh showed a relatively low value(86~199 mV) compared to other areas. Most groundwaters belonged to Ca-(Na)-$HCO_3$ type, and groundwaters of metamorphic rock exhibited the highest concentration of Na, Mg, Ca, Cl, $NO_3$-N, U, and those of plutonic rock showed the highest concentration of $HCO_3$, and Rn. Uranium and fluoride from granite areas did not show any correlation. However, uranium and bicarbonate displayed a positive relation of some areas in plutonic rocks($R^2$=0.3896).

• Textile Coloration and Finishing
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• v.29 no.3
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• pp.115-121
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• 2017

Demands for nylon suede as an artificial leather are increasing due to its functionality and aesthetics. To achieve high value added nylon suede based on green technology, this study was carried out in order to obtain useful data for the nylon suede fabrics with eco-friendly dyeing process by a pad-steam method instead of a dip dyeing process using sulphur black dye to reduce the industrial waste of water. The dyeability of the nylon suede was investigated according to reducing temperatures, dye concentrations, and reducing agent's concentrations. Throughout the results of the CIE $L^*a^*b^*$ and Munsell values, the optimized dyeing conditions of the nylon suede using sulphur black dye are $70^{\circ}C$ of dyeing temperature, 30% o.w.f. of dye concentration, and $9g/{\ell}$ of reduction agent concentration, respectively. Furthermore washing colorfastness, light colorfastness and perspiration colorfastness were achieved in the range of 4-5 grades.

• Journal of Korean society of Dental Hygiene
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• v.14 no.4
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• pp.485-493
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• 2014

Objectives : The study investigated and analyzed the relationship between health-related lifestyle and psychosomatic self-reported symptom in dental hygiene students. Methods : A self-reported questionnaire was filled out by 478 dental hygiene students in Gyeonggi-do and Gangwon-do from March 7 to June 21, 2012 by random sampling method after informed consent. Results : The health-related lifestyle showed the results as follows. Nonsmokers accounted for 89.1%. Those who never exercise accounted for 67.9% and 37.9% of the students sleep for 5-6 hours. Those who take alcohols twice per month accounted for 58.2%. The correlation between the symptoms and lifestyle included multiple subjective symptoms(I), respiratory(A), eyes and skin(B) and digestive organs(C) symptoms and smoking status(p<.01), mouth and anal(D), depression(K), nervousness(E). There existed the correlation in average sleeping time(p<.01) and impulsivess(H) and smoking status(p<.05) and lie scale(L) and regular exercise(p<.05) and aggressiveness(F) and drinking habits and irregular and life(G) and breakfast habits. The smoking habit, sleeping time, and snack intake had an influence on psychosomatic self-reported oral health-related symptoms. Conclusions : Cessation of smoking, adequate sleeping time, and reduction of snack intake can improve the oral health-related lifestyle and reduce the self-reported symptoms in the dental hygiene students.

• Korean Journal of Animal Reproduction
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• v.13 no.3
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• pp.179-187
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• 1989

The experiment was carried out to study the profile of uterine specific protein during early pregnancy in sows and to test it's immunosuppressive activity. Uterine protein samples were obtained by flushing the uterine horn on Day 3, 6, 9, 12 and 15 of the estrous cycle and the pregnancy respectively and the protein concentration of each sample was determined. The change of uterine protein was examined by sodium dodecyl sulfate(SDS)-PAGE. The immunosuppressive activity of uterine secretory protein was investigated according to the lymphocyte blastogenesis response to mitogen. The results of this experiment are summarized as follows ; 1. The uterine protein during estrous cycle and early pregnancy was relatively constant up to Day 9, but increased on Day 12. Maxium total protein values were found on Day 15. The concentration of serum proteins were about 82-95 mg during estrous cycle, but decreased to about 70-82 mg during early pregnancy. 2. The proteins components similar electrophoretic patterns(PAGE) that were no differences (band ; a, b, c, d, e, f, g, I) on Days 3, 6 and 9 of the estrous cycle and pregnancy. But there were more 2 bands specifically on Day 12 of the pregnancy and on Day 15 of estrous cycle and showed more 4 bands on Day 15 of early pregnancy. They seemed to be acidoprotein and their average molecular weight were 38,000, 22,300 and 12,600. 3. When uterine protein were added 500$\mu\textrm{g}$/ml, there was no immunosuppresive activity on Day 3 of estrous cycle and lymphocyte blastogenesis was slightly suppressed on Day 3 of pregnancy. The immunosuppressive activity on Day 9 of estrous cycle and pregnancy appeared in 500$\mu\textrm{g}$/ml and 150$\mu\textrm{g}$/ml, respectively the uterine protein on Day 12 and 15 showed immunosuppresive activity, which at the level of 150$\mu\textrm{g}$/ml during non-pregnancy and at the level of 100 to 125$\mu\textrm{g}$/ml during early pregnancy, respectively.

• 한국정보컨버전스학회:학술대회논문집
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• 2008.06a
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• pp.63-70
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• 2008

The authors, Tom Karygiannis of NIST, and Bernard Eydt, Greg Barber, Lynn Bunn, and Ted Phillips of Booz Allen Hamilton, wish to thank Steven Fick, Rick Korchak, Kate Remley, Jeff Guerrieri, Dylan Williams, Karen Scarfone, and Tim Grance of NIST, and Kenneth Waldrop and Beth Mallory of Booz Allen Hamilton. These individuals reviewed drafts of this document and contributed to its technical content. The authors would also like to express their thanks to several experts for their critical review and feedback on drafts of the publication. These experts include V.C. Kumar of Texas Instruments; Simson Garfinkel of the Naval Postgraduate School; Peter Sand of the Department of Homeland Security; Erika McCallister of MITRE; and several professionals supporting Automatic Identification Technology(AIT) program offices within the Department of Defense(DoD), especially Nicholas Tsougas, Fred Naigle, Vince Pontani, Jere Engelman, and Kathleen Smith. During the public comment period we received helpful comments from the following Federal Government agencies: the US Departments of Defense, Health and Human Services, Homeland Security, Labor, and State; the Office of the Director of National Intelligence; the Office of Management and Budget; and the General Services Administration. We also received several helpful contributions from commercial industry, including comments from EPCglobal, VeriSign, and Priway. Finally, the authors wish to thank the following individuals for their comments and assistance: Brian Tiplady, Daniel Bailey, Paul Dodd, Craig K. Harmon, William MacGregor, Ted Winograd, Russell Lange, Perry F. Wilson, John Pescatore, Ronald Dugger, Stephan Engberg, Morten Borup Harning, Matt Sexton, Brian Cute, Asterios Tsibertzopoulos, Mike Francis, Joshua Slob in, Jack Harris, and Judith Myerson.