• Korean Journal of Plant Resources
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• v.33 no.6
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• pp.689-695
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• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2021.04a
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• pp.35-35
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• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• Journal of The Korean Association For Science Education
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• v.19 no.4
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• pp.570-584
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• 1999

The units about electricity in 5th and 6th science curriculum for elementary school was analyzed and the elementary students' understanding of related concepts was investigated. This study was to make connection between the research about alternative concepts and writing textbooks. It was found that students' response had following characteristics. They had strong belief that the + and - ends of battery should be connected. However this belief was not complete because students did not understand that the other circuit elements had to be connected properly. When the circuit involve more than one bulbs or batteries, they counted the number of batteries or bulbs only and paid little attention to the connection of them. In explaining circuits and the brightness of the bulbs in the circuits. students tried to explains the circuit using scientific terms but failed to used them properly. Although the concept of resistance was not dealt explicitly. student had reasonable qualitative understanding about resistance. According to response of students, several suggestions might be made for the curriculum. The structure and the circuit elements and the proper connection needs to be taught. Also more emphasis is necessary to pay attention to the connection in addition to the numbers of bulbs or batteries. Finally, it needs to be investigated carefully if it is better to introduce some qualitative model to explain the amount of current through bulb depending on the connection and the number of bulbs. It might also be better to introduce some terms, such as current and resistance with the model.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2000.11a
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• pp.771-774
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• 2000

Coiling processes of filaments need precise work and standardization. It is important to maintain equal pitch of filaments. Uniform pitch of filaments is one of the dominant elements of life time and efficiency of bulbs. First coiling process of filament wires is modeled by nonlinear contact problem between filaments and mandrel. Analysis of coiling process using finite element method is conducted to consider manufacturing parameters and pitch distance is calculated under the given conditions. Also image detecting system is developed to inspect uniformity of pitch. This system will be used to inspect quality of filaments during coiling processes.

• Applied Biological Chemistry
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• v.24 no.4
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• pp.218-223
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• 1981

This study was conducted to investigate the effects of post-harvest drying method and subsquent storage condition on the quality of garlic bulbs for 10 months from July, 1980 to April, 1981. The 27% weight loss of garlic bulbs by HPHD (hot air post-harvest frying) for 12 days at $40^{\circ}C$ (8hrs/day) was equal to that by NPHD (conventional natural post-harvest drying) for 35 days. But the decay occured 5.5% only in NPHD. During the storage period of garlic bulbs by HPHD, their decay and weight ]oss were less 25.4% and 13.5% in ambient storage, and less 14.2% and 7.5% in low temperature storage than those of NPHD. When garlic bulbs were stored in low temperature, the weight loss and decay were less 20.0% and 22.4% in NPHD, and tess 14.0% and 9.9% in HPHD than those in ambient temperature storage. The Quality of garlic bulbs packed with 0.08mm polyethylene film stored at ambient temperature for 2 months was so poor as to be inedible because of the adverse effect of $CO_2$ and the growth of molds, but in low temperature storage for 10 months it was in good shape showing the weight loss, the decay and the sprouting 2.6%, 3.4% and 26.8%, respectively.

• The Plant Pathology Journal
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• v.17 no.3
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• pp.141-148
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• 2001

Postharvest decay of onion bulbs was examined by inspecting the commercial packages in the market or in storage. Bulb rot incidence was unexpectedly high, and onion bulbs with 1st quality grade were rotten most severely by 51%, followed by 32% for 2nd and 21% for 3rd grades. This indicates that larger bulbs had higher incidences of bulb rots. Major pathogens associated with basal and neck rots were Fusarium oxysporum and Aspergillus sp. or Botrytis allii, respectively, of which basal rot was most prevalent and damaging during storage. Among the epiphytic microorgani는 from onion plants, several Bacillus and Paenibacillus spp. and previously selected Pseudomonas putida and Trichoderma harzianum had inhibitory efficacy against bulb rot pathogens. Among these B. amyloliquefaciens BL-3, Paenibacillus polymyxa BL-4, and P. putida Cha 94 were highly inhibitory to conidial germination of F. oxysporum and B. allii. P. putida Cha 94, B. amyloliquefaciens BL-3, P. polymyxa BL-4, and T. harzianum TM were applied in the rhizoplane of onion at transplanting. Initially antagonist populations decreased rapidly during the first one month. However, among these antagonists, rhizoplane population densities of BL-3, Cha 94, and TM were consistently high thereafter, maintaining about 10$^4$-10$^{5}$ cells or spores per gram of onion root up to harvest time. The other bacterial antagonist BL-4 survived only for two months. TM was the most effective biocontrol agent against basal rot, with the number of rotten bulbs recorded at 4%, while that of the control was 16%. Cha 94 was effective for the first 20 days, but basal rot increased thereafter and had about the same control efficacy as that of BL-3 and BL-4. When the antagonists were applied to the topping areas of onion bulbs at harvest, TM was the most effective in protecting the stored onion bulbs from neck rotting. The second effective antagonist was BL-3. TM and BL-3 completely suppressed the neck rot in another test, suggesting that biocontrol of postharvest decay of onion using these microorganisms either at the time of transplanting or at harvesting may be promising.

• Journal of applied mathematics & informatics
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• v.25 no.1_2
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• pp.109-118
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• 2007

A parametric boundary equation is established for the principal period-2 bulb in the cubic Mandelbrot set. Using its geometry, an efficient escape-time algorithm which reduces the construction time for the period-2 bulbs in the cubic Mandelbrot set is introduced and the implementation graphic results display the fascinating fractal beauty.

• 한국정보디스플레이학회:학술대회논문집
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• 2002.08a
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• pp.359-363
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• 2002

Several innovations for reducing the bulkiness of glass bulbs for flat and large CRTs have been made and further technologies are being developed by using material and structural approaches. The integrated glass technology based on these innovations will provide a lightweight shallow glass bulb for the realization of a half- depth CRT.

• Natural Product Sciences
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• v.4 no.3
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• pp.148-152
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• 1998

The extract from the bulbs of Pancratium maritimum L. afforded 12 alkaloids belonging to the skeletally six different groups of the Amaryllidaceae alkaloids. In this paper, the isolation and identification of (-)-N-demethyl-galanthamine (1), (+)-tazettine (2) and (-)-2-O-demethylmontanine (3) are described. Their structures have been determined by using extensive spectroscopic techniques. This is the first report describing the occurrence of 1 and 3 in this plant.

• Korean Journal of Agricultural Science
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• v.42 no.4
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• pp.305-311
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• 2015

In this research, we surveyed the yield change of garlic cultivation with seed bulbs according to annual field culture after induced meristem culture. From 2007 to 2013, yearly produced seed bulbs from $2^{nd}$ to $8^{th}$ generation were cultivated in the Muan field. Over the generations, the general growth characteristics such as the plant height, leaf number, leaf length and stem diameter were all decreased. When we compared the conventional seed bulbs and TCR ones, the plant height and stem diameter showed bigger difference. And the TCR seed bulbs showed a week late harvest time against the conventional ones. The yield of $2^{nd}$ generation was 72.3 g and 57.1 g for the $8^{th}$ generation. However every generation showed higher yield than conventional seed garlic's ones as 43.9 g. The yield index showed the same trend as $2^{nd}-8^{th}$ to 63% to 21%. After the tissue culture of garlics, the viral infections (OYDV and GSV) of TCR was far less than that of conventional ones. In case of LYSV, the infection ratio was 6.7% at $2^{nd}$ generation and 26% at $8^{th}$. However, the ratio was far less than that (80%) of the conventional ones.