• 한국자원식물학회지
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• 제33권6호
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• pp.689-695
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• 2020

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'Milky way'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 µl PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2021년도 춘계학술대회
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• pp.35-35
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• 2021

This study was conducted to improve and supplement the system of cryopreservation for adventitious bulbs induced by tissue cultured bulb-scales of lily (Lilium spp.) cvs. 'MilkyWay'. The explants, bulblets and bulb-scale-bulblets, were treated to low temperature (4℃) for 7 days prior to the pre-culture. The adventitious bulbs were pre-cultured in Murashige and Skoog (MS) liquid medium supplemented with sucrose (0.3 and 0.7M). The pre-cultured adventitious bulbs were treated to loading solution (LS1 or LS2, C4 or C6) containing 35% of PVS3 (LS1, C4) or 40% of PVS3 (LS2, C6) for 40 min and exposed to dehydration solution (PVS3, B1) containing 50% glycerol and 50% sucrose for 60 min at 25℃. The adventitious bulbs were moved onto droplets containing 3 ㎕ PVS3 on sterilized aluminum foils, and then soaked into liquid nitrogen (LN) for 60 min. The result of highest regrowth rate as 65.7% was obtained in cold treatment (4℃), osmoprotected with LS1 solution, and cultured in PCM3 medium by using bulb-scale-bulblet for cryopreservation. This result shows that droplet-vitrification could be used as a promising method for long-term storage of lily genetic resource.

• 한국과학교육학회지
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• 제19권4호
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• pp.570-584
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• 1999

The units about electricity in 5th and 6th science curriculum for elementary school was analyzed and the elementary students' understanding of related concepts was investigated. This study was to make connection between the research about alternative concepts and writing textbooks. It was found that students' response had following characteristics. They had strong belief that the + and - ends of battery should be connected. However this belief was not complete because students did not understand that the other circuit elements had to be connected properly. When the circuit involve more than one bulbs or batteries, they counted the number of batteries or bulbs only and paid little attention to the connection of them. In explaining circuits and the brightness of the bulbs in the circuits. students tried to explains the circuit using scientific terms but failed to used them properly. Although the concept of resistance was not dealt explicitly. student had reasonable qualitative understanding about resistance. According to response of students, several suggestions might be made for the curriculum. The structure and the circuit elements and the proper connection needs to be taught. Also more emphasis is necessary to pay attention to the connection in addition to the numbers of bulbs or batteries. Finally, it needs to be investigated carefully if it is better to introduce some qualitative model to explain the amount of current through bulb depending on the connection and the number of bulbs. It might also be better to introduce some terms, such as current and resistance with the model.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.771-774
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• 2000

Coiling processes of filaments need precise work and standardization. It is important to maintain equal pitch of filaments. Uniform pitch of filaments is one of the dominant elements of life time and efficiency of bulbs. First coiling process of filament wires is modeled by nonlinear contact problem between filaments and mandrel. Analysis of coiling process using finite element method is conducted to consider manufacturing parameters and pitch distance is calculated under the given conditions. Also image detecting system is developed to inspect uniformity of pitch. This system will be used to inspect quality of filaments during coiling processes.

• Applied Biological Chemistry
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• 제24권4호
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• pp.218-223
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• 1981

한국산 6쪽마늘의 장기 저장에서 수확후(收穫後) 예건방법과, 저장고내온습도(貯藏庫內溫濕度) 조건이 품질(品質)에 미치는 영향에 대하여 1980년(年) 7월(月)부터 1981년(年) 4월까지 조사시험 하였던바, 수확후 열풍예건한 마늘은 자연 예건된 것에 비하여 상온저장 조건에서 중량감소와 부패발생에서 각각 25.4%와 13.5% 정도 억제 효과가 있었으며, 저온저장 조건에서는 14.2%와 7.5% 정도 경감되었다. 그리고, 저온저장 $(0^{\circ}C)$ 하므로 상온저장한 것에 비하여 얼풍예건 처리한 마늘의 경우 중량감소와 부피발생이 각각 14.0%와 9.9% 정도 줄었으며, 자연예건한 마늘에서는 각각 20.0%와 22.4% 경감되는 효과를 보였다. 또한, 열풍예건처리와 저온저장조건은 저장중 마늘의 발아 및 호흡억제와 allin성분의 보존에도 좋은 효과가 있었다.

• The Plant Pathology Journal
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• 제17권3호
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• pp.141-148
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• 2001

Postharvest decay of onion bulbs was examined by inspecting the commercial packages in the market or in storage. Bulb rot incidence was unexpectedly high, and onion bulbs with 1st quality grade were rotten most severely by 51%, followed by 32% for 2nd and 21% for 3rd grades. This indicates that larger bulbs had higher incidences of bulb rots. Major pathogens associated with basal and neck rots were Fusarium oxysporum and Aspergillus sp. or Botrytis allii, respectively, of which basal rot was most prevalent and damaging during storage. Among the epiphytic microorgani는 from onion plants, several Bacillus and Paenibacillus spp. and previously selected Pseudomonas putida and Trichoderma harzianum had inhibitory efficacy against bulb rot pathogens. Among these B. amyloliquefaciens BL-3, Paenibacillus polymyxa BL-4, and P. putida Cha 94 were highly inhibitory to conidial germination of F. oxysporum and B. allii. P. putida Cha 94, B. amyloliquefaciens BL-3, P. polymyxa BL-4, and T. harzianum TM were applied in the rhizoplane of onion at transplanting. Initially antagonist populations decreased rapidly during the first one month. However, among these antagonists, rhizoplane population densities of BL-3, Cha 94, and TM were consistently high thereafter, maintaining about 10$^4$-10$^{5}$ cells or spores per gram of onion root up to harvest time. The other bacterial antagonist BL-4 survived only for two months. TM was the most effective biocontrol agent against basal rot, with the number of rotten bulbs recorded at 4%, while that of the control was 16%. Cha 94 was effective for the first 20 days, but basal rot increased thereafter and had about the same control efficacy as that of BL-3 and BL-4. When the antagonists were applied to the topping areas of onion bulbs at harvest, TM was the most effective in protecting the stored onion bulbs from neck rotting. The second effective antagonist was BL-3. TM and BL-3 completely suppressed the neck rot in another test, suggesting that biocontrol of postharvest decay of onion using these microorganisms either at the time of transplanting or at harvesting may be promising.

• Journal of applied mathematics & informatics
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• 제25권1_2호
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• pp.109-118
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• 2007

A parametric boundary equation is established for the principal period-2 bulb in the cubic Mandelbrot set. Using its geometry, an efficient escape-time algorithm which reduces the construction time for the period-2 bulbs in the cubic Mandelbrot set is introduced and the implementation graphic results display the fascinating fractal beauty.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2002년도 International Meeting on Information Display
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• pp.359-363
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• 2002

Several innovations for reducing the bulkiness of glass bulbs for flat and large CRTs have been made and further technologies are being developed by using material and structural approaches. The integrated glass technology based on these innovations will provide a lightweight shallow glass bulb for the realization of a half- depth CRT.

• Natural Product Sciences
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• 제4권3호
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• pp.148-152
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• 1998

The extract from the bulbs of Pancratium maritimum L. afforded 12 alkaloids belonging to the skeletally six different groups of the Amaryllidaceae alkaloids. In this paper, the isolation and identification of (-)-N-demethyl-galanthamine (1), (+)-tazettine (2) and (-)-2-O-demethylmontanine (3) are described. Their structures have been determined by using extensive spectroscopic techniques. This is the first report describing the occurrence of 1 and 3 in this plant.

• 농업과학연구
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• 제42권4호
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• pp.305-311
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• 2015

In this research, we surveyed the yield change of garlic cultivation with seed bulbs according to annual field culture after induced meristem culture. From 2007 to 2013, yearly produced seed bulbs from $2^{nd}$ to $8^{th}$ generation were cultivated in the Muan field. Over the generations, the general growth characteristics such as the plant height, leaf number, leaf length and stem diameter were all decreased. When we compared the conventional seed bulbs and TCR ones, the plant height and stem diameter showed bigger difference. And the TCR seed bulbs showed a week late harvest time against the conventional ones. The yield of $2^{nd}$ generation was 72.3 g and 57.1 g for the $8^{th}$ generation. However every generation showed higher yield than conventional seed garlic's ones as 43.9 g. The yield index showed the same trend as $2^{nd}-8^{th}$ to 63% to 21%. After the tissue culture of garlics, the viral infections (OYDV and GSV) of TCR was far less than that of conventional ones. In case of LYSV, the infection ratio was 6.7% at $2^{nd}$ generation and 26% at $8^{th}$. However, the ratio was far less than that (80%) of the conventional ones.