• Journal of Plant Biotechnology
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• v.29 no.3
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• pp.179-183
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• 2002

This study was carried out to investigate the influence of medium composition for in vitro mass propagation of Lycoris squamigera Max. After the disks of short stems, segments of leaf within bulb and scale were cultured on MS basal medium supplemented with various plant growth regulators, they were examined for the extent of callus formation, shoot and root regeneration. In the culture of stem disks, adventitious shoots were regenerated from the basal tissue of bulb scales, and combined medium of 1.0 mg/L 2,4-D or NAA＋2.0 mg/L BA or kinetin showed the the best response and 4∼6 shoots per explant formed. In the culture of leaf segments within bulbs, both MS medium supplemented with 1.0 mg/L NAA＋2.0 mg/L TDZ and with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA were produced callus profusely on the base of leaf tissue and 3∼6 shoots were regenerated per explant. In the scale segments culture, calli were produced on the basal tissue on medium with 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. The best result were shown on MS medium with 1.0 mg/L NAA＋2.0 mg/L TDZ, and 1.0 mg/L 2,4-D＋1.0∼2.0 mg/L BA. Maximum number of regenerated shoots was up to 10∼12. Adventitious root formation from explants were formed profusely on MS medium with 1.0 mg/L NAA＋2.0 mg/L kinetin. The most desirable method for mass propagation of plantlets was the shoot regeneration from scale segments then subsequently subcultured on medium for rooting.

• Journal of Applied Biological Chemistry
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• v.59 no.2
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• pp.129-132
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• 2016

This study was conducted to evaluate the attraction effects of Sitophilus zeamais and Tribolium castaneum to light emitting diode (LED) trap in granary and compared with the black light bulb (BLB) trap, which is typical used in commercial trap. The red LED trap showed more attractive to S. zeamais and T. castaneum than that of the BLB. Moreover, the external condition of granary was about 1.5 times more attractive to S. zeamais and T. castaneum than the internal condition of granary. These results suggested that red LED trap could be useful to control S. zeamais and T. castaneum in granary.

• Korean Journal of Plant Tissue Culture
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• v.28 no.6
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• pp.341-346
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• 2001

To investigate the effects of MS medium strength and nitrogen concentration on bulblet formation from bulblet scale segment culture and bulblet growth from bulblet of Lilium oriental hybrid 'Casa Blanca', asiatic hybrid 'Mona', and longiflorum hybrid 'Hinomoto', 0.5∼2.0 strength of MS salts and 30∼120 mM nitrogen concentrations of MS medium were examined in vitro. The number of bulblets from bulb scale segment was favored in the strength of 0.5∼1.0 strength of MS salts or 30 mM total nitrogen concentration of MS medium in three cultivars. But the growth of bulblets formed in vitro was promoted in the 2 strength of MS medium or hight concentration of total nitrogen of MS medium up to 120 mM in three experimented cultivars.

• International Journal of Industrial Entomology and Biomaterials
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• v.14 no.2
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• pp.81-85
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• 2007

White Muscardine is the most common fungal disease of silkworm, Bombyx mori L. caused by the pathogenic fungus, Beauveria bassiana (Balsamo) Vuillemin. In the present investigation, an attempt has been made to screen locally available medicinal/ weed plants against Beauveria bassiana. Among the plant extracts (PE) tested, 5% aqueous crude extract of the bulb of Allium sativum (Garlic) has been found to be most effective against Beauveria bassiana. The radial growth of Beauveria bassiana in vitro was inhibited to the tune of 54.9% in aqueous extract and 54.4% in ethanolic extract of Allium sativum and correspondingly mycelial dry weight gave rise to 110.7 mg and 108.7 mg against 201.7 mg in control 15 days post treatment. Similarly, silkworm larvae topically inoculated with the Beauveria bassiana conidia ($1.8{\times}10^6/ml$) registered survival up to 53.0% against 0.0% in control after treatment with aqueous extract of Allium sativum. Simultaneously, as a preventive measure, silkworm larvae were put to rear in conidia contaminated seat paper instantly treated with aqueous extract of Allium sativum that also increased survival up to 61.0% against 4.6% in control. It is also observed that the plant extract is absolutely innocuous to silkworm.

• Korean Journal of Breeding Science
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• v.42 no.3
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• pp.294-297
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• 2010

A new intermediate parent 'Wonye 30001' (Allium cepa L.) was developed by Bioenergy Crop Research Center, NICS in 2009. As a male sterile line, 'Wonye 30001' could be used for hybrid seed production by crossing with pollen parent. The first cross was conducted in 2000. Male sterile plants were selected from 'Ginque' breeding lines and fertile plants were selected from YG-1-1. The male sterile line 'Wonye 30001' has circular bulb and bulb weight of 260 g. As mid-late maturing type, lodging date is around May 20. Plant height and stem diameter are 57 cm and 15.9 mm, respectively. In seed harvesting characteristics, number of flower stalks and the length are 6 and 135cm, respectively. Flowering date of 'Wonye' is May 23 and is completely male sterile. 'Wonye 30001' is a promising male sterile line for hybrid bulb onion seed production.

• Korean Journal of Pharmacognosy
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• v.6 no.3
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• pp.125-129
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• 1975

The pharmacognostical and pharmacological studies of 'Manryong' were carried out, which has been widely used in Chun-Ra Province, Korea and the following results were obtained. 1) The original plant of the bulb MANRYONG is Erythronium japonicum DECNE. (Liliaceae). 2) The internal structure consists of mostly parenchyma containing numerous starch grains, vascular bundles and substituted fibers. 3) The external surface occurs as a yellowish white and slightly bented ovoidal shape. 4) 'Manryong; contains 11 kinds of free amino acids, such as asparagin, tryptophan, cystine, glutamic acid, threonine, glycine, leucine, proline, histidine, methionine, and alanine. 5) Hemolytic action of the crude saponin isolated from 'Manryong' is weaker than that of digitonin. 6) Effects of 'Manryong' extracts on ceruloplasmin were studied and an antidotal activity of the extracts was found in liver intoxicated with carbon tetrachloride.

• Korean Journal of Agricultural and Forest Meteorology
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• v.21 no.4
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• pp.250-260
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• 2019

This study assessed clove germination, shoot growth, photosynthesis and bulb development of southern-type garlic (Allium sativum L.) in a temperature gradient tunnel (TGT), to examine the impacts of increases in temperature on the growth of garlic and find a way to minimize them. The temperatures in the middle and outlet of the TGT were 3.2℃ and 5.8℃ higher, respectively, than the ambient temperature at the tunnel inlet. The germination of garlic cloves was late at temperatures of ambient+3℃ (in the middle of the TGT) and ambient+6℃ (at the outlet) than at ambient temperature (at the inlet). However, bolting and the timing of maximum leaf number per plant were faster at ambient+3℃ or +6℃ than at ambient temperature. Shoot growth was generally greater at ambient temperature. Bulb growth did not significantly differ according to cultivation temperatures, but fresh and dry weights were slightly higher at ambient temperature and ambient+3℃ in the late growth stage. The photosynthesis rate (A), stomatal conductance (g_s), and transpiration rate (E) were higher at ambient+3℃ than at ambient temperature. Furthermore, at ambient+3℃, the net photosynthetic rate (A_max) was high, while the dark respiration rate (R_d) was low. At ambient temperature and ambient+3℃, bulb development was healthier, resulting in better productivity and more commercial bulbs, while at ambient+6℃, the bulbs were small and secondary cloves developed, resulting in low commercial value. Therefore, at elevated temperatures caused by global warming, it is necessary to meet the low-temperature requirements before clove sowing, or to delay the sowing time, to improve germination rate and increase yield. The harvest should also be advanced to escape high-temperature stress in the bulb development stage.

• Korean Journal of Agricultural Science
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• v.41 no.4
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• pp.299-302
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• 2014

A freesia (Freesia hybrida Hort.) 'Shiny Bell' was developed for the cut flower in the National Institute of Horticultural Herbal Science in 2012. This hybrid was selected from a crossing between cn. 'Teresa' and 'Rossini', which is white semi-double flowering and red double flowering, respectively. in 2004. Morphological characteristics of the selected hybrid were investigated for 3 years from 2009 to 2011, and then it was named 'Shiny Bell'. 'Shiny Bell' had red color of RHS W155C, and double flower. The growth of the plant was vigorous and the average height was 109.3 cm. The average flower width was 5.8 mm, number of floret per stalk was 11.3, and stalk length was 8.5 cm. The average yield, 7.3 Branches per plant, was 3 stems per plant more than the control cultivar. The average days to first flowering of 'Shiny Bell', was 143.3 days, and it was approximately 7 days later than the control cultivar. The average yield of 'Shiny Bell' was 4.0 cormlets per plant.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.305-309
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• 2013

A freesia (Freesia hybrida Hort.) 'Dancing Flame' was developed for the cut flower in the National Institute of Horticultural Herbal Science in 2007. This hybrid was selected from a crossing between a seedling 'Vulcano' and 'Sailor', which is scarlet double flowering and purple single flowering, respectively, and 'Figaro' which is bright red color and semi-double flowering, in 2000. Morphological characteristics of the selected hybrid were investigated for 3 years from 2005 to 2007, and then it was named 'Dancing Flame'. 'Dancing Flame' had red color (RHS R44B) and single flower with yellow center color (RHS Y9A). The growth of the plant was vigorous and the average height was 93.3 cm. The average flower width was 60 mm, number of floret per stalk was 14.3, and stalk was 13.2 cm length. The average yield, 7.8 cuttings per plant, was 2.5 stems per plant more than the control cultivar. The average days to first flowering of 'Dancing Flame', 133 days, was approximately 5 days earlier than the control cultivar. And its average yield, 7.1 cormlet per plant, was also 2.5times more than the control cultivar.

• Horticulture, Environment, and Biotechnology : HEB
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• v.59 no.6
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• pp.875-888
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• 2018

Tulip (Tulipa L.) is one of the most important ornamental geophytes in the world. Analysis of molecular variability of tulips is of great importance in conservation and parental lines selection in breeding programs. Of the 70 genic microsatellites, 15 highly polymorphic and reproducible markers were used to assess the genetic diversity, structure, and relationships among 280 individuals of 36 wild and cultivated tulip accessions from two countries: Iran and the Netherlands. The mean values of gene diversity and polymorphism information content were 0.69 and 0.66, respectively, which indicated the high discriminatory power of markers. The calculated genetic diversity parameters were found to be the highest in wild T. systola Stapf (Derak region). Bayesian model-based STRU CTU RE analysis detected five gene pools for 36 germplasms which corresponded with morphological observations and traditional classifications. Based on analysis of molecular variance, to conserve wild genetic resources in some geographical locations, sampling should be performed from distant locations to achieve high diversity. The unweighted pair group method with arithmetic mean dendrogram and principal component analysis plot indicated that among wild tulips, T. systola and T. micheliana Hoog exhibited the closest relationships with cultivated tulips. Thus, it can be assumed that wild tulips from Iran and perhaps other Middle East countries played a role in the origin of T. gesneriana, which is likely a tulip species hybrid of unclear origin. In conclusion, due to the high genetic variability of wild tulips, they can be used in tulip breeding programs as a source of useful alleles related to resistance against stresses.