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Histological Comparison Study with Primo Node and Immature Liver Tissue on Liver Surface in Rat (흰쥐의 간 장기 표면에서 관찰되는 프리모 노드와 미성숙 간조직과의 조직학적 비교연구)

  • Ryu, Yeon-Hee;Lee, Sung-Won;Hwang, Sung-Yeoun;Kim, Min-Su;Ban, Hyo-Jeong;Choi, Sun-Mi;Kim, Jae-Hyo;Sohn, In-Chul;Ahn, Seong-Hun
    • Korean Journal of Acupuncture
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    • v.28 no.1
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    • pp.47-52
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    • 2011
  • 목적 : 프리모 모드와 미성숙 간조직에 대한 조직학적 특성을 비교하고자 하였다. 방법 : 흰쥐에서 간절제한 후, 간장기 표면에서 관찰되는 프리모 모드와 그와 유사한 미성숙 간조직을 H&E, Oil red O, Masson trichrome and van Gieson 염색방법을 통해서 비교 연구하였다. 결과 : 상기 실험 연구를 진행한 결과, 다음과 같은 결론을 얻었다. 1. 조직의 일반적인 특징을 관찰할 수 있는 H&E 염색결과, 프리모 노드에서는 많은 수의 조그마한 동(sinus)이 관찰되었고 적혈구나 영양을 공급하는 혈관의 분포는 관찰되지 않았다. 이와 반대로 미성숙 간조직에서는 동이 관찰되지 않았으며 혈관의 분포와 적혈구가 관찰되었다. 2. 양 조직의 지방성분의 유무를 관찰하기 위한 Oil red O 염색결과, 프리모 노드에서는 지방성분이 관찰되지 않았으나 미성숙 간조직에서는 관찰되었다. 3. 양 조직의 콜라겐성분의 유무를 관찰하기 위한 Masson trichrome and van Gieson 염색결과, 프리모 노드에서는 약간의 콜라겐 성분이 관찰되었으나 elastic 성분은 관찰되지 않았으며, 미성숙 간조직에서는 콜라겐 성분과 elastic 성분이 관찰되지 않았다. 결론 : 상기 결과는 프리모 노드와 간조직과는 조직학적 성질이 다른 것으로 사료되며, 특히 콜라겐 성분의 적은 결과는 프리모 노드가 불규칙한 형태를 이루고 있는 이유에 대한 실마리를 제공하였다. 이러한 결과는 프리모 노드의 특성에 대한 기본 자료로 활용될 수 있을 것이다.

Anti-microbial, Anti-oxidant, and Anti-thrombosis Activities of the Lees of Bokbunja Wine (Rubus coreanus Miquel) (복분자주 주박의 항균, 항산화 및 항혈전 활성)

  • Kim, Mi-Sun;Kang, Dong-Kyoon;Shin, Woo-Chang;Sohn, Ho-Yong
    • Journal of Life Science
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    • v.25 no.7
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    • pp.757-764
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    • 2015
  • The immature fruit of Rubus coreanus Miquel (bokbunja in Korean) is mainly consumed as a fruit wine due to its sour taste and low sugar content. The lees (LBW) remaining after the production of bokbunja wine are discarded as they have no specific usage. The aim of this study was to develop high-value-added biomaterials for functional foods and beauty/health products by investigating the anti-microbial, anti-oxidant, and anti-thrombosis activities of LBW using ethanol and hot water extracts and their subsequent organic solvent fractions. The ethyl acetate (EA) fraction of LBW extracts has a high polyphenol content (413–459 mg/g), and showed strong anti-microbial activity against gram-positive bacteria. The EA fraction also showed excellent radical-scavenging activity against DPPH anion, ABTS cation, and nitrite, with strong reducing power. The polyphenol-enriched EA fraction strongly inhibited thrombin, prothrombin, and blood coagulation factors. The butanol fraction showed a specific inhibition of coagulation factors, as measured in activated partial thromboplastin time assay, which is linked to intrinsic blood coagulation. The butanol fraction also showed strong inhibition of platelet aggregation, at levels comparable to aspirin. The residue of the hot-water extract, which is produced by sequential solvent fractionation of the LBW extract, showed superior inhibition against platelet aggregation when compared to aspirin. Our results suggest that the LBW, which are currently discarded, are a promising source of novel functional foods and beauty/health products.

Distribution of Invasive Species in Metropolitan Busan, South Korea (생태계교란식물의 부산광역시 분포 실태)

  • Ryu, Tae-Bok;Lim, Jeong-Cheol;Lee, Cheol-Ho;Kim, Eui-Ju;Choi, Byoung-Ki
    • Journal of Life Science
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    • v.27 no.4
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    • pp.408-416
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    • 2017
  • This research aimed to identify the diversity and the distribution of invasive alien plant species in the metropolitan city of Busan, South Korea. According to our results, we discovered 10 species of invasive alien plants distributed in Busan, demonstrating that this urban area has a high domestic plant diversity. A cluster analysis identified that the dominant communities of Aster pilosus, Lactuca serriola, Ambrosia artemisiifolia, Rumex acetosella and Solanum carolinense were highly similar in species composition. Different species of invasive alien plants tended to occur together in dominant communities, indicating their preference for shared habitats. The most extensively distributed species in Busan were Lactuca serriola (16 districts), followed by Ambrosia artemisiifolia (11 districts), Aster pilosus (11 districts) and Rumex acetosella (10 districts). The administrative districts with the most diverse invasive alien plants were Gangseo-gu (8 species) and Buk-gu (8 species), which are both areas with high human interference and diverse habitats. Additional environmental information was collected for these species' habitats in Busan. The results of this research can be used to assess the current status of invasive alien plants in Busan and can provide basic data useful for effectively controlling and preventing the spread of invasive plants.

Mineral Composition and Physiological Activities of Methanol Extract from the Seeds of Persicaria tinctoria (쪽 종자의 영양성분과 메탄올 추출물의 생리활성 효과)

  • Park, Yun-Jum;Cheon, Gil-Yong;Song, Hyun-Woo;Shin, Chang-Sik;Ku, Yang-Gyu;Kang, Na-Ru;Heo, Buk-Gu
    • Korean Journal of Plant Resources
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    • v.29 no.1
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    • pp.32-38
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    • 2016
  • In order to improved use of Persicaria tinctoria seeds and to get basic information, general composition, fatty acid, mineral, amind acid analysis and physiological activity of methanol extract of Persicaria tinctoria seeds were investigated. Total calories of Persicaria tinctoria seeds were 348.00 kcal/100 g, general composition, carbohydrate, crude protein, crude fat and crude ash consisted of 7.85%, 67.90%, 10.10%, 4.00% and 10.15%, respectively. The amount of saturated and unsaturated fatty acids was showed 0.9048 g/100 g and 2,714 g/100 g, respectively. Minerals contained 100g of Persicaria tinctoria seeds were followed by K (549.5 ㎎), Mg (264.4 ㎎), Ca (216.2 ㎎), Fe (12.1 ㎎), Zn (3.0 ㎎). Total 15 kinds of amino acids were detected, these amino acids displayed higher value in the alanine (1,432.6 ㎎/100 g) and glutamic acid (1,088.8 ㎎/100 g). Total polyphenol and flavonoid contents were 11.08 ㎎/L and 3.56 ㎎/L, respectively. DPPH radical scavenging and ABTS radical scavenging activity in the methanol extract of 1,000 ㎎/L was showed 86.74% and 61.74%, respectively.

Yield and Size of Enlarged Rhizome among Different Kinds of White Lotus (백련 종류에 따른 연근 크기와 수확량)

  • Im, Myung-Hee;Park, Yong-Seo;Cho, Ja-Yong;Park, Sam-Gyun;Yun, Jae-Gill;Jang, Hong-Gi;Heo, Buk-Gu
    • Korean Journal of Plant Resources
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    • v.22 no.1
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    • pp.42-48
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    • 2009
  • This study was conducted to examine the difference in yield and size of enlarged rhizome according to the kinds of native white lotuses. Four white lotuses (Nelumbo nucifera) were collected from 4 different sites, Jeonbuk Jeonju ('Garam'), Jeonnam Muan ('Hoesan'), Chungnam Buyeo ('Ihnchuisa'), and Chungnam Cheongyang('Cheongyang'). Each enlarged rhizome was planted in an area of 3.3 $m^2$ on 10. April, 2007 and digged out on 19. February, 2008. The yield of enlarged rhizome was in the order of Hoesan (3.0 kg/$m^2$), Cheongyang (2.5 kg/$m^2$), Garam (2.0 kg/$m^2$) and Ihnchuisa (1.0 kg/$m^2$). The number of enlarged rhizomes was in the order of Ihnchuisa (4.9/$m^2$), Garam (4.3/$m^2$), Hoesan (4.2/$m^2$) and Cheongyang (4.1/$m^2$). Total number of nodes in the enlarged rhizomes were much more in the order of Hoesan (17.0/$m^2$), Ihnchuisa (16.0/$m^2$), Cheongyang (15.3/$m^2$) and Garam (14.1/$m^2$). Component ratio of enlarged rhizome length below 50cm for Ihnchuisa, Garam, Hoesan and Cheongyang were 76.3%, 53.0%, 38.2% and 40.3% respectively. Native white lotus had a longer enlarged rhizome in the first node than the other nodes, and their enlarged rhizome length and width became thin. And it became thick and short from the second node of enlarged rhizome. Accordingly the amount of enlarged rhizomes 'Hoesan' was extremely much than others.

Star Building Materials Study on Wood Structure and Combustion Characteristics (건축재료별 목재구조와 연소특성에 관한연구)

  • Kim, Jong-Buk;Park, Young-Ju;Lee, Si-Young
    • Fire Science and Engineering
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    • v.30 no.5
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    • pp.60-66
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    • 2016
  • This study investigated the structure and combustion characteristics of four species, Timber Douglas-fir being used construction materials (finishes), Lauan, Cryptomeria japonica, Pinus densiflora trees in the area. In lookong into the ignition characteristics was a time range of ignition (TTI) appeared in the 21 s~32 s, especially Pinus densiflora TTI is ignited in a relatively rapid 601 s to 21 s than the other materials were destroyed in the 631 s. The maximum heat release rate and average heat release rate is Pinus densiflora > Lauan > Cryptomeria japonica > Douglas-fir showed a net. Barrel emissions are Lauan > Douglas-fir > Pinus densiflora > Cryptomeria japonica was in order. The total emissions was postponed Pinus densiflora $424.80m^2/m^2$, Lauan $185.93m^2/m^2$. Douglas-fir carbon emissions of 1460, showing 0.185 kg/kg CO maximum value from s $CO_2$ values show the maximum value to 15,986 kg/kg in 750 s stopped in the 3,090 s. Next, the study suggested methods to utilize as the basic data for evaluating the safety of the fire as a building material.

Isolation and Characteristics of a Bacterium Removing Chemical Softener, Organo-polysiloxane (화학유연제, Organopolysiloxane 분해세균의 분리 및 특성)

  • Lee, Jung-Hun;Son, Dong-Chul;Kim, Jung;Kim, Hyun-Soo;Yu, Tae-Shick
    • Korean Journal of Microbiology
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    • v.36 no.2
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    • pp.119-124
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    • 2000
  • Thirty three strains of bacteria were isolated from the wastewater and soil contaminated a chemical softener, nrganopolysiloxane. Of these, five strains which showed higher activities for removal this chemical were finally selected for further study. By five strains the 2,500 mgll chemical softener was removed 65.2-67.9% at $37^{\circ}C$ for 5 days by shaking. The pH optimum for growth of W3721, S3712, and S3723 strain were at around pH 7.0-7.5, and W2811, and W2823 strain were at pH 6.5-7.0, respectively. The temperature optimum for growth of W3712 strain was at $37^{\circ}C$ and the other four strains were at TEX>$30^{\circ}C$. The optimal pH and temperature for removal by W3712 strain was initial pH 7.0 and $37^{\circ}C$ respechvely. The W3712 strain was identified and named as Corynebacterium pseudodiphtheriticum W3712 based on its morphological and physiological characteristics.

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Effect of the Application of Microbubbles and/or Catalyst on the Sludge Reduction and Organic matter of Livestock Wastewater (마이크로버블과 촉매 적용에 따른 가축분뇨의 슬러지와 유기오염물질 감량 효과)

  • Jang, Jae Kyung;Kim, Min Young;Sung, Je Hoon;Chang, In Seop;Kim, Tae Young;Kim, Hyun Woo;Kang, Young Koo;Kim, Young Hwa
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.10
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    • pp.558-562
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    • 2015
  • This study was tested to evaluate the effect of the six different combinations of microbubble, catalyst, and air as oxidant on the sludge and organic matter reduction. When all of microbubbles and catalyst, and an oxidizing agent (under Conditions 1) such as air were used, the sludge was removed more than 99%, and TCOD and SCOD removal was 58% and 13%, respectively. This result was the highest value of six conditions. In the following order, the sludge reduction of the microbubbles with air (Condition 2) and catalyst with air (condition 4) was 95% and 93.1%, respectively. TCOD removal was found to be each 53% and 47%. When the microbubbles were used with oxidant like air, the removal of sludge and organic matter was high. All of these values were higher than that of using only microbubbles and catalyst without air. In the microbubbles and catalyst react under air supply condition, OH-radicals were generated in the reaction process. These OH-radicals in the reaction process decomposed the pollutants by the strong oxidizing power. In all conditions with air, the sludge reduction was high removal rate more than 93% and TCOD removal was over 47%.

Changes in Water Potential of Pine Seedlings Inoculated with Bursaphelenchus xylophilus (소나무재선충 접종목의 수분포텐셜 변화)

  • Lee, Hwa-Yong;Koo, Chang-Duck;Sung, Joo-Han;Shin, Joon-Hwan;Yoo, Jong-Hyun
    • Journal of Korean Society of Forest Science
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    • v.99 no.3
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    • pp.337-343
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    • 2010
  • The purpose of this study was to understand water stress development in pine seedlings inoculated with Bursaphelenchus xylophilus. Leaf water potentials of four years old Pinus densiflora seedlings inoculated with the nematode were measured with a plant moisture system at predawn and midday. The midday leaf water potentials on the 35th day after the inoculation were -1.04 MPa in the infected seedlings, while -0.94 MPa in the non-infected seedlings. The predawn water potentials on the 56th day were -0.71 MPa in the infected, while -0.26 MPa in the non-infected. At this time the leaves of the infected seedlings became yellow and resin did not exude on the cut basal stem. Density of the nematode was maximum on the 28th day, but dropped sharply on the 68th day. The phloem and pith tissues became brown and the resin exudation reduced in the infected seedlings. In conclusion, pinewood nematode inoculation developed water stress in pine seedlings in a month.

The Images of the Elderly Perceived by Mid and Older-aged Adults and Their Preparation for Later Life (중장년층이 인식하는 노인 이미지와 노후생활 준비도)

  • Yeo, Yeon-Jung;Kim, Jin-Sook
    • The Journal of the Convergence on Culture Technology
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    • v.6 no.2
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    • pp.257-262
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    • 2020
  • This study recruited adults aged 35 to 64 living in Daegu and Gyeongsang buk-do to analyze the images of the elderly perceived by the mid and older- aged adults and their preparation for later life. The results of this study are as follows: First, as for the images of elderly people recognized by the research subjects, psychological images were the most positive, followed by physical and social images. Those with a higher education level, full-time job, and parents alive had more positive images of the elderly. The psychological images were better in those married compared to singles or divorcees, and the higher the age considered as elderly, the better the psychological and social images of the elderly. Second, preparation for later life in the mid and older-aged adults was better in order of emotional and physical preparation, whereas economic and leisure and social preparation were not enough. Those who are female, those with a higher education level, higher average monthly household income, professional job, and full-time job, and those who have an older age in mind as a definition of elderly have been better prepared for later life. Third, it was found that the sub-factors of their images of elderly people and preparation for later life affected each other, and the more positive their images of elderly people, the better they had been prepared for later in life. The results of this research suggests a desirable direction for improving the images of the elderly, implicating the necessity of exploring measures to provide individual and social support and developing educational programs for successful life after retirement.