• Journal of Veterinary Science
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• 제21권1호
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• pp.13.1-13.14
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• 2020

Currently, the systems for culturing buffalo spermatogonial stem cells (SSCs) in vitro are varied, and their effects are still inconclusive. In this study, we compared the effects of culture systems with undefined (foetal bovine serum) and defined (KnockOut Serum Replacement) materials on the in vitro culture of buffalo SSC-like cells. Significantly more DDX4- and UCHL1-positive cells (cultured for 2 days at passage 2) were observed in the defined materials culture system than in the undefined materials system (p < 0.01), and these cells were maintained for a longer period than those in the culture system with undefined materials (10 days vs. 6 days). Furthermore, NANOS2 (p < 0.05), DDX4 (p < 0.01) and UCHL1 (p < 0.05) were expressed at significantly higher levels in the culture system with defined materials than in that with undefined materials. Induction with retinoic acid was used to verify that the cultured cells maintained SSC characteristics, revealing an SCP3+ subset in the cells cultured in the defined materials system. The expression levels of Stra8 (p < 0.05) and Rec8 (p < 0.01) were significantly increased, and the expression levels of ZBTB16 (p < 0.01) and DDX4 (p < 0.05) were significantly decreased. These findings provided a clearer research platform for exploring the mechanism of buffalo SSCs in vitro.

• 한국생물정보학회:학술대회논문집
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• 한국생물정보시스템생물학회 2005년도 BIOINFO 2005
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• pp.5-5
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• 2005

• 대한치주과학회:학술대회논문집
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• 대한치주과학회 1998년도 제38회 종합학술대회 연제초록
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• pp.9-9
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• 1998

• The Korean Journal of Physiology
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• 제22권2호
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• pp.153-161
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• 1988

• Kyungpook Mathematical Journal
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• 제12권1호
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• pp.153-157
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• 1972

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1994년도 교수 연수회(역학)
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• pp.250-260
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• 1994

• 전산구조공학
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• 제34권1호
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• pp.4-9
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• 2021

• Asian-Australasian Journal of Animal Sciences
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• 제5권4호
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• pp.617-622
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• 1992

Four rumen-fistulated swamp buffaloes steers were used in a change-over experiment. This experiment was designed to provide a comparison of the effect of urea-ammonia treatment of rice straw with untreated rice straw. Nitrogen, mineral and trace elements were supplied at adequate levels to both diets in order to overcome deficiencies which may have otherwise confounded a direct comparison. There was a 46% increase in the intake of digestible organic matter (OM) with the urea-ammonia treated diet. This was contributed by a 17% increase in the digestibility of OM and a 25% increase in the voluntary intake of OM. Of the cell wall fraction, the digestibility of hemicellulose increased by the greatest amount (26%). There was an increased rate of passage of particulate matter out of the rumen for the treated straw, along with the increased rate of OM fermentation resulted in a 9% decrease in the amount of digesta dry matter (DM) contained in the rumen. The volatile fatty acid (VFA) pool in the rumen was 24% higher for the treated diet.

• 물과 미래
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• 제20권4호
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• pp.285-294
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• 1987

본 연구는 댐파괴나 급격한 방류로부터 발생되는 홍수파가 하도내에서 사류의 흐름으로 나타날 때, 이를 해석하기 위한 수치모형을 개발한 것이다. 기본방정식으로서는 dynamic wave equation을 사용하였고 이식을 general Preissmann sceme에 의하여 유한차분화 시켰으며 fore-sweep algorithm에 의하여 해석하였다. 본 모형을 Buffalo-Creek flood의 경우에 적용하고 실측치 및 DAMBRK에 의한 계산 결과치와 비교검토하여 모형의 적용성을 입증하였다. 국내 효기댐의 경우에 대하여서는 파괴당시의 실측자료를 기초로한 유출수문곡선을 유도하였고 댐 하류부의 홍수파 전달양상을 해석하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제15권3호
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• pp.326-329
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• 2002

A population of exotic Holstein Friesian, Jersey, their crossbreds and the indigenous Murrah breed of buffalo bulls (n=486), used in artificial insemination breeding program were screened for the allelic distribution of the ${\kappa}$-casein and ${\beta}$-lactoglobulin genotypes. The preferred "B" allele frequency was highest in Murrah buffalo bulls followed by Jersey and Holstein Friesian. The increase in this particular allele frequency in the Holstein Friesian crossbred bulls was more when compared to their Jersey counterparts. Hardy-Weinberg's equilibrium was maintained albeit with some deviations, which was higher in crossbreds than in purebreds. The feasibility of using such large-scale molecular diagnostic tools in the field and their significance with regards to the dairy economy is discussed.