• Journal of Species Research
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• v.13 no.3
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• pp.288-292
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• 2024

In the course of our survey of microbial diversity, a total of seven basidiomycetous yeast strains were isolated from flowers sampled across the four provinces of the Republic of Korea, of which two strains were identified as an unrecorded species. Comparative analysis was conducted for identification of strains using the D1/D2 region of the large subunit(LSU) rRNA gene sequences, observation of cell morphologies, and carbon assimilation tests. Seven strains were classified within the phylum Basidiomycota. Two unrecorded strains, JRP1-10 (KACC 410703) and KC-1 (KACC 410704), were identified as Vishniacozyma pseudocarnescens, belonging to the family Bulleribasidiaceae, order Tremellales, class Tremellomycetes. Two strains had ellipsoidal-shaped and monopolar budding cells and smooth and cream-colored colonies after cultivation at 25℃ for 3 days. Here, we present the morphological and biochemical characteristics of the unrecorded yeast species, Vishniacozyma pseudocarnescens, in the Republic of Korea.

• Journal of Bio-Environment Control
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• v.28 no.1
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• pp.46-54
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• 2019

Experiments of local cooling and heating on crown and root zone of forcing cultivation of strawberry 'Seolhyang' using heat pump and root pruning before planting were conducted. During the daytime, the crown surface temperature of the crown local cooling treatment was maintained at $18{\sim}22^{\circ}C$. This is suitable for flower differentiation, while those of control and root zone local cooling treatment were above $30^{\circ}C$. Budding rate of first flower clusters and initial yields were in the order of crown local cooling, root zone local cooling and control in root pruning plantlet and non pruning plantlet, except for purchase plantlet. Those of root pruning plantlet were higher than those of non pruning plantlet. These trends were evident in the yield of the first flower cluster until February 14, 2018, and the effect of local cooling and root pruning decreased from March 9, 2018. The budding rates of the second flower cluster according to the local cooling and root pruning treatments were not noticeable compared to first flower cluster but showed the same tendency as that of first flower cluster. In the heating experiment, root zone local heating(root zone $20^{\circ}C$+inside greenhouse $5^{\circ}C$) and crown local heating(crown $20^{\circ}C$+inside greenhouse $5^{\circ}C$) saved 59% and 65% of heating fuel, respectively, compared to control(inside greenhouse $9^{\circ}C$). Considering the electric power consumption according to the heat pump operation, the heating costs were reduced by 55% and 61%, respectively.

• Journal of Bio-Environment Control
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• v.32 no.4
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• pp.449-455
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• 2023

In this study, the effect of crown heating on the cultivation environment, budding, flowering and yields of strawberry was analyzed. In December, January, and February, when the outside temperature was low, the average strawberry crown temperature at daytime in the test zone was 1.3℃ higher than that in the control zone, and the average strawberry crown temperature at nighttime in the test zone was 2.7℃ higher than that in the control zone. The average bed temperature at daytime in test zone was 1.7℃ higher than that in the control zone, and the average bed temperature at nighttime in test zone was 2.4℃ higher than that in the control zone. As a result of performing correlation analysis and regression analysis on strawberry crown temperature and budding period, the correlation coefficient was -0.86, which tended to be shorter as the crown temperature was higher, and the determination coefficient was 0.74. The total yields of strawberry during test period were 392.6 g/plant for test greenhouse and 346.0 g/plant for control greenhouse respectively. As for the quality of strawberries, the ratio of 2L (very large) grades and L (large) grades was 62.4% in the test greenhouse and 58.5% in the control greenhouse, indicating that the proportion of high quality strawberries was higher in the test greenhouse.

• Applied Microscopy
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• v.26 no.4
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• pp.465-477
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• 1996

The influence of dehydrocholic acid, cholesterol and phosphstidylcholie to the fine structure of vacuolar apparatus was investigated to better understand the mechanism of intracellular transport of bile constituents in the hepatocytes of rats. The cis Golgi cisterns faced toward the bile canaliculi both in normal and experimental groups. In the hepatocytes from the rats of experimental groups, the primary organic solutes in bile influence the Gogi apparatus, ER and lysosome in the way of increase, cisternal dilation or budding to form the vacuoles. In the dehydrocholic acid group, the cis Golgi cisterns appeared to be sacculated and showed buds, which were probably separated to be vacuoles. Some of the vacuoles appeared to be fused to the bile canaliculi. In the cholesterol and phosphatidylcholine groups, the Golgi cisterns appeared to be dilated and lysosomes were increased in the vicinity of bile canaliculi. The cis Golgi cisterns showing linear saccular fashions were occasonally observed. The increase of lysosomes were more predominant in the cholesterol group. The evidence suggests that dehydrocholic acid is mainly transported through the ER and cis Golgi cisterns, and cholesterol and phosphatidylcholine are mainly transported through the ER and lysosomes via the trans Golgi cisterns, but the cholesterols are frequently transported via the lysosomes.

• Korean Journal of Agricultural Science
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• v.40 no.4
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• pp.297-303
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• 2013

This study was carried out to examine the effect of Red LED (660 nm) and fluorescent lamp for night break (NB) treatments of each 3 hours (22:30-01:30), 4 hours (22:00-02:00) and 5 hours (21:30-:02:30) per day for 53 days on flower bud initiation and growth in Chrysanthemum cv. 'Baekma' and cv. 'Jinba'. The days to flower budding after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (21.0 days) and 5 hr (20.5 days) NB, and it was shorter at Red LED 3 hr (14.2 days). The days to flowering after short-day treatment in 'Baekma' was longer at fluorescent lamp 4 hr (54.0 days), 5 hr (53.5 days) NB, and Red LED 5 hr (53.3 days), and it was shortest at Red LED 3 hr (50.2 days) NB treatment among all treatments. The days to flower budding after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (20.6 days) and was shorter at Red LED 3 hr (14.1 days) among all treatments. Similarly, the days to flowering after short-day treatment of 'Jinba' was longer at fluorescent lamp 4 hr (55.3 days) and was shortest at Red LED 3 hr (50.2 days) among all treatments. Therefore, inhibition of flower bud initiation was the most effective under fluorescent lamp 4 hr treatment. The length of cut flower of 'Baekma' was increased by fluorescent lamp 4 hr, 5 hr, and Red LED 5 hr, but of 'Jinba' was longer at LED 4 hr and 5 hr treatment. The weight of cut flower of 'Baekma' was heaviest at fluorescent lamp 5 hr treatment and was at Red LED 5hr treatment for 'Jinba' even though there was not statistically significant difference between 'Baekma' and 'Jinba'. Consequently, under fluorescent lamp 4 hr for night break was the most effective on flower bud initiation, flowering inhibition and cut-flower characteristics in 'Baekma' and 'Jinba'.

• Korean Journal of Weed Science
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• v.9 no.1
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• pp.28-33
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• 1989

This study was examined for the effect of tuber size, bud and tuber removal, and tuber cutting on growth of Eleockaris kuroguwai Ohwi. Elongation of plumule and radicle, and the weights of tuber, rhizome and shoot were increased as tuber size is large. The weights of shoot and tuber reduced by bud planting without tuber at 15 days after budding, however number of tubers were increased compared with normal tuber. When cross-cutting tubers were seeded, the weights of shoot, rhizome and tuber were produced as 84%, 85%, 71%, respectively compared with normal tuber, and the number of tubers was not influenced. The weights of tuber, rhizome and shoot were lowed as the bud removal, and there were a great number of tubers at bud removal of 15 days after budding. Tuber of E. k, contained as nonfibrous 78.9gr, phosphorous 77mg, iron 16.8mg, niacin 1.3mg per 100gr edble portion. The positive correlations were observed between shoot weight and tuber weight, and number of tubers.

• Korean Journal of Microbiology
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• v.35 no.2
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• pp.158-163
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• 1999

A ploygalacturonase-produchg yeast was isolated from Cheju soil by selective eivichment media. One strain which has the highesl activity of polygalacturonase was selected. The characle~ishcs of the strain CS-2 were as follows: CS-2 utilized xylose. sucrose, maltose, u.ehalose, cellobiose. melibiose, lactose, raffinose, inosiiol, dulicilol, and dextrose, but did not utilized galactose, nitrate. nit~te, and lysine. Growth of CS-2 was inhibited by cyclohexamide, 1% acetic acid, and high concenaation (over 50%) of glucose. It grew at $30^{\circ}C$ but did 'IIOL $35^{\circ}C$. The cell size ofthe strain CS-2 was 2.9 p ~ n in length and 1.3 $\mu$ in diameter. Vegetable reproductmn was multiple budding and ascospre was present I to 4. Pseudomycelia or true myceliua formation were not observed In any of the cullureq. These results suggest that strain CS-2 is most likely a strain related Cryptococcus spp. (Cryptococcu spp. CS-2). When polygalacturonase or ihe yeast was induced by addition of polygalactoronic acid, polygalacturonase activity was detected in culture supernatent. There was a peak of specific activity a1 he mid-stationary phase(3 days culture) of growth. Polygalacturonase specific activity of Crylmcoccus sp. CS-2 was 2.96 unitsling. The molecular weighl ol'polygalacturonase was showed to be 46 KDa by both SDS-PAGE and activity stailling.

• Journal of the Korean association of regional geographers
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• v.19 no.3
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• pp.446-453
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• 2013

Festivals using plant phenology is one of the examples can represent the ecosystem services. Changwon Jinhae Gunhangje is typical spring festival using cherry blossoms phenology. This research was done to define relationship between flowering of cherry blossoms and Gunhangje. Phenological data (Julian day) of cherry blossoms and visitor number of Gunhangje from 1987 to 2013 were collected. Differences of Phenological phase of cherry blossoms and duration of Gunhangje was analyzed. Trend analysis was performed to know changes of flowering and budding day of cherry blossoms. Correlations between visitor number of Gunhangje and environmental factors was defined by factor analysis and regression analysis. Results are showed that during the 26 years, periodicity of flowering and budding day was not identified or even identified it was not significant. Environmental factors effect on visitor numbers of Gunhangje were defined as temperature factor, day factor, and precipitation factor. Temperature factor was significantly correlated with visitor numbers of Gunhangje. Though Flowering day of cherry blossoms and festival duration was not matched, however, difference of visitor numbers was not showed. These results suggest that fixed duration of Gunhangje would be more effective to visitors than changing the festival duration following the phenological day changes of cherry blossoms.

• Korean Journal of Agricultural Science
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• v.13 no.2
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• pp.157-167
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• 1986

The study was carried out to identify several factors affecting isolation and culture of cotyledone and leaf mesophyll protoplasts of cabbage (Brassica oleraceae), petsai (B.campestris subsp. pekinensis) and rape (B.napus). High viable protoplasts could be obtained when the cotyledon and the leaf mesophyll tissue of all species were treated with enzyme solution composed of 1% macerozyme 'R-10', 1.5% Onozuka 'R-10', 10% mannitol and 50,0 mM $CaCl_22H_2O$ for 4 hours. The protoplasts which obtained from the cotyledon of all species except the cabbage and the leaf mesophyll tissue of all species were divided on NN culture medium supplemented with 9.1% mannitol, 1% glucose, 1% sucrose, $1mg/{\ell}$ 2,4-D, $0.5mg/{\ell}$ NAA and $0.5mg/{\ell}$ BA. The division of the rape leaf mesophyll protoplasts were continued and led to colony.

• Journal of Veterinary Clinics
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• v.30 no.2
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• pp.115-118
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• 2013

An 8-year-old spayed female domestic shorthair cat was presented with a chief complaint of chronic nasal discharge and dyspnea. Physical examination revealed pyohemorrhagic nasal discharge, inspiratory dyspnea and stertor, and an enlarged right mandibular lymph node. Abnormalities of blood works and serum chemistry included mildly increased hematocrit, and globulin concentration. Serologic tests for FeLV and FIV, and a panel of polymerase chain reaction tests for Chlamydophila felis, Feline Calicivirus, Herpesvirus, Bordetella, Mycoplasma felis, and H1N1 influenza was all negative. Only radiographic finding showed increasing soft tissue density in the right nasal cavity and computed tomography disclosed soft tissue/fluid opacification in the right nasal cavity, paranasal sinus, and pharyinx along with slight deviation to the right of the osseous nasal septum. Focal lysis of ventral nasal septum was also suspected in CT scan. Cytological evaluation of fine needle aspirate smears of the enlarged mandibular lymph nodes revealed numerous fungal yeasts having variably thick capsule both extracellularly and intracellularly with low numbers of macrophages. Some yeasts showed narrow based budding, which was a consistent finding with Cryptococcus organisms. Serum protein electrophoresis was a polyclonal consistent with chronic infection and serum was submitted for a fungal serology panel test. In serologic tests Cryptococcus antigen titer was 1 : 32,768. In vitro culture was unsuccessful. Treatment was initiated with administration of fluconazole, clindamycin, and tocopherol. Clinical signs resolved within 3 days after the initial treatment. The cat was discharged and scheduled for periodic evaluation and continued therapy, but was lost to follow-up thereafter.