• Title/Summary/Keyword: brown spots

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Halo Blight of Kudzu Vine Caused by Pseudomonas syringae pv. phaseolicola in Korea

  • Jeon, Yong-Ho;Chang, Sung-Pae;Kim, Sang-Gyu;Kim, Young-Ho
    • The Plant Pathology Journal
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    • v.22 no.2
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    • pp.119-124
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    • 2006
  • Kudzu vine(Pueraria montana var. lobata) is an invasive climbing woody vine that envelops trees and shrubs, pressing physically and shutting out sunlight, which needs to be controlled. Kudzu vine pathogens were surveyed as a way to seek its biocontrol agents in 2002. Occurrence of a bacterial halo blight disease of kudzu vine was observed at several localities in Korea including Euiwang and Suwon in Gyeonggi Province, Daejon, and Gochang and Buan in Jeonbuk Province. Symptoms of brown to black spots with a surrounding yellowish halo appeared from June and lasted till the rainy season without much expansion, but accompanying often leaf blight and defoliation. Isolated bacteria were identified as Pseudomonas syringae pv. phaseolicola based on physiological and cultural characteristics, Biolog, fatty acid and 16S rDNA sequencing analyses. In artificial inoculation test, these bacteria produced the same halo spot symptoms on kudzu vine and bean plants. They also induced hypersensitive responses (HR) on tobacco, tomato, and chili pepper leaves. This is the first report of a bacterial disease of kudzu vine in Korea, and the bacterial pathogen can be used as a biocontrol agent against the pest plant.

Pathological studies on exudative epidermitis in experimentally infected pigs I. Macroscopical and histopathological observations (실험적 돼지 삼출성 표피염에 관한 병리학적 연구 I. 육안적 및 병리조직학적 관찰)

  • Oh, Kyu-shil;Lee, Cha-soo
    • Korean Journal of Veterinary Research
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    • v.34 no.4
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    • pp.787-799
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    • 1994
  • To elucidate morphologic lesion of porcine exudative epidermitis which is occurred sporadically in Korea, Staphylococcus hyicus subsp. hyicus isolated from the naturally affected pigs was inoculated to suckling pigs. The infected piglets were observed grossly and histopathologically. Although affected piglets were taking acute, subacute, or chronic course, some piglets suffered from chronic disease showed poor prognosis and marked growth depression. Affected peglets had erythematous skin on the face, ear, and abdomen and these localized lesions appear as brownish spots of exudative epidermitis and fromed crust in the early stage. But, after this stage, the skin were covered by viscous greasy exudate and formed blackish brown crust and appeared fissures and hypertrophy. Grossly, there has been hemorrhage with the removal of crust-like materials of epidermis and edematous subcutis. The superficial lymph nodes were edematous and swollen or congested and hemorrhagic. Some piglets had swollen ureters, cysts in the renal cortex, or polyarthritis. A few cases had mild edematous swelling of kidney, intestinal catarrh and congestion of brain. Microscopically, skin lesions had detachment of keralinized layer and parakeratosis of epidermis, hydropic degeneration of epidermal cell, and retrogressive degeneration of hair root sheath. Dermis had edema, and infiltration of neutrophils and mononuclear cells. As the disease was proceeded, there was marked perivasculitis with lots of mononuclear inflammatory cells. More chronic lesions formed granuloma-like bodies(nodules) due to more mononuclear, perivascular inflammatory cell infiltration and proliferation of fibroblast. Lots of plasma cells and eosinophils were also present in dermis. Epidermis was hyperplastic by proliferation of basal cells stratum germinativum and epidermal pegs often extended into the dermis. In secondary infection, lots of neutrophils could be seen in epidermis and derms. Kidney had neutrophilic infiltration, necrotic and cystic glomeruli, and dilation of renal tubules and ureters. Purulent arthritis was sometimes observed in joints. Three days old mice administrated Staphylococcus hyicus subsp hyicus subcutaneously before had focal congestion and hemorrhage, necrosis, and subcutaneous edema of the skin. This observation was also seen in the study of mice administrated exfoliatin toxin of Staphylococcus which evoked human staphylococcal scalded skin syndrome.

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A Study on Morphology and Behavior of the Sapsaree : A Korean native dog (Canis familiaris)

  • Han, Kook-Il;Alam, Mahboob;Lee, Yun-Mi;Lee, Dong-Hoon;Ha, Ji-Hong;Kim, Jong-Joo
    • Journal of Animal Science and Technology
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    • v.52 no.6
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    • pp.481-490
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    • 2010
  • This study was conducted to characterize the morphological and behavior patterns of Sapsaree dogs. The population (n=8,256) has been constructed since 1990 over 12 generations and managed at the Sapsaree Breeding Research Institute, Gyeongsan, Gyeongbuk province. Eighteen morphological and seven behavioral traits were investigated for 882 individuals. Linear models were applied for each trait by fitting sex, season of birth, hair color or test age. The averages of body weight, body length, body height, and depth of chest were $20.5{\pm}2.4\;kg$, $57.3{\pm}4.2\;cm$, $52.1{\pm}3.6\;cm$, and $21.1{\pm}2.4\;cm$, respectively. Males had greater estimated values than females for these body conformation traits. The chocolate Sapsarees had greater averages for body weight, body height and chest depth. The older animals (>2 years) had heavier body weight than younger animals. About 54, 69, 97, 39 and 83.3% of the Sapsarees had hairs with yellow color, straight, medium to long, untangled, and longer around eyes, respectively. Also, about 40% brown eye, 43% curly tail, 78% normal jaw, 86% no missing teeth, 90% no dewclaw, and >90% with black nose, pendent ear, tongue without spots were observed. About 95% males were normal in testicles state. The males performed significantly better than the females for majority of the behavioral traits. For nerve stability, affability, wariness, adaptability, sharpness, activity, and reaction during blood drawn about 79%, 73%, 76%, 61%, 70%, 48% and 81% of the Sapsarees performed at desired level. In general, the Sapsarees showed good characteristics for both morphological and behavioral traits, which can be exploited to use the Sapsaree breed as a companion or guide dog.

Reproductive characteristics, egg and larval development of short ninespine stickleback, Pungitius kaibarae

  • Hwang, In Joon;Lee, Si Woo;Han, Young Sim;Kim, Kyeong Hwan
    • Fisheries and Aquatic Sciences
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    • v.24 no.11
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    • pp.375-382
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    • 2021
  • In this study, the reproductive behavior and embryonic and larval development of the short ninespine stickleback Pungitius kaibarae was described and illustrated based on observations during spawning, hatching, and larval rearing trials. Adult P. kaibarae were collected downstream in Jinhae during the reproductive season (April-May). Males had nuptial coloration on their entire black bodies, with blue dorsal spines and yellow eyes, whereas females had a brown spotted pattern on their bodies. Males built nests on the stems of water weeds and attracted females. Fertilization occurred in the nest immediately after spawning, and males guarded the eggs until hatching. The fertilized eggs of P. kaibarae were spherical, demersal, adhesive, and transparent, and each egg measured 1.43 ± 0.07 mm in diameter. The morula, blastula, and gastrula stages, as well as hatching began at 5, 18.5, 21.5, and 96 post fertilization (HPF), respectively, at 20.0 ± 0.5℃. The newly hatched larvae had a total length (TL) of 5.67 ± 0.50 mm, with a yolk volume of 0.583 ± 0.059 mm3. Their mouths and anuses had not yet opened. At 2 days posthatching (days post hatching, DPH), the yolk was completely absorbed and the larvae began to feed exogenously. Pigmentation was observed in freshly hatched larvae 4 h after hatching, with the presence of eight areas with a dotted pattern on the dorsal surface of the larvae and dispersed spots on the head and yolk sac. At 30 DPH, the TL of the juveniles was 21.34 ± 1.70 mm. The nest area and number of eggs were 259.56 ± 101.39 mm2 (75.18-506.04) and 155.33 ± 114.12 (0-437), respectively.

A Study of the Characteristics of Painting Materials Used in Welcome Feast for the Pyeongan Governor: Focusing on Banquet at Yeongwangjeong Pavilion (평안감사향연도(平安監司饗宴圖)의 채색 재료 특성 연구 -연광정연회도(練光亭宴會圖)를 중심으로-)

  • Park, Jin Ho;Chang, Yeon Hee;Ko, Soo Rin
    • Conservation Science in Museum
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    • v.28
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    • pp.109-136
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    • 2022
  • This study analyzes the , one of the three panels of 《Welcoming Banquets for the Governor of Pyeong-an》, a documentary painting of the late Joseon Dynasty, with the aim to identify the coloring materials used in the painting. The painting was first imaged at each wavelength in order to minimize the potential problems in the process of analyzing specific parts. This study applied X-rays to identify ink, gold, and organic and inorganic pigments and used infrared rays to find ink and copper-based pigments. It also applied hyperspectral imaging to distinguish organic pigments from black, blue, and green materials. It also analyzed spots selected for each color to identify the following materials: white lead (white), ink/indigo (black), a combination of red lead and cinnabar (red), pink dye, purple dye, iron oxides (brown), orpiment/dye (yellow), malachite/malachite and yellow dye/indigo (green), azurite/white lead and indigo/indigo (blue), indigo and cochineal (violet), and gold leaf (gold). It is expected that more efficient analysis will be made possible by securing a sufficient library for each wavelength.

Fine Mapping of Zenith Derived Rice Stripe Virus Resistance Gene, Stv-b

  • Sais-Beul Lee;Jun-Hyun Cho;Nkulu Rolly Kabange;Sumin Jo;Ji-Yoon Lee;Yeongho Kwon;Ju-Won Kang;Dongjin Shin;Jong-Hee Lee;You-Cheon Song;Jong-Min Ko;Dong-Soo Park
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2020.12a
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    • pp.63-63
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    • 2020
  • Rice stripe virus (RSV) disease is one of the major constraints in rice production, transmitted by the small brown planthopper (SBPH; Laodelphax striatellus). Upon RSV infection, plants develop typical symptoms, which include chlorosis and weakness of newly emerged leaves, white and yellow spots, stripe on leaves, and necrotic and wilting leaves, resulting in plant growth inhibition, oxidative damage that may culminate in programmed cell death (PCD) and plant death in severe epidemics. Although RSV-resistant quantitative trait loci (QTLs), Stv-a, Stv-b, and Stv-bi, were mapped using various resistant varieties, one RSV-resistant gene, OsSOT1, has been identified so far. In this study, we used the rice cultivar Zenith, known to carry Stv-b, to investigate novel RSV-genes through fine mapping. Therefore, we crossed Zenith (Donor parent, RSV resistant) with Ilpum (Recurrent parent, RSV susceptible) to fine-map using a BC2F2 population of 2100 plants. Chromosome segment introgression lines that were heterozygous at a different region were selected, two types of heterozygous lines showed an heterozygous genotype between Sid2 and Sid75 to Indel9 and RM6680. Interestingly, we identified qSTV11Z region harboring Stv-b, covering about 171-kb region between the InDel markers Sid75 and Indel8. The localization of qSTV11Z provides useful information that could be used for marker-assisted selection and determination of genetic resources in rice breeding.

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First Report of Anthracnose Caused by Colletotrichum sojae on Peanut in Korea (Colletotrichum sojae에 의한 땅콩 탄저병 발생 보고)

  • Shinhwa Kim;Soo Yeon Choi;Hyunjung Chung;Nak Jung Choi;Bo Yoon Seo;Sang-Min Kim
    • The Korean Journal of Mycology
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    • v.52 no.1
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    • pp.55-60
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    • 2024
  • In August 2023, leaf spot disease was observed in peanuts in Cheongju-si, Korea. Leaf spots occurred at the leaf margins and the lesions gradually expanded. Diseased leaf areas were light or dark brown and irregular in shape. A fungal isolate was obtained from symptomatic leaf and cultured on potato dextrose agar (PDA) medium at 25℃. An isolate was identified as Colletotrichum sojae based on morphological characteristics and sequences of the internal transcribed spacers, glyceraldehyde-3-phosphate dehydrogenase, chitin synthase-1, actin, and 𝛽-tubulin genes. Pathogenicity tests were performed on peanut seedlings in a conidial suspension (1×106 conidia/mL). Lesions were observed on the peanut leaf 5 d after inoculation. The pathogen was re-isolated from the lesions of the inoculated leaves. To the best of our knowledge, this is the first report of anthracnose on peanut caused by C. sojae in Korea.

Banana Blast Caused by Pyricularia angulata Hashioka (Pyricularia anguiata Hashioka에 의한 바나나 도열병 (가칭))

  • Kim Wan Gyu;Kim Chang Kyu;Lee Eun Jong
    • Korean Journal Plant Pathology
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    • v.3 no.2
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    • pp.114-119
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    • 1987
  • Banana blast was frequently encountered in greenhouses of Jeju province during a local survey of plan diseases from 1985 to 1986. The disease incidence on banana was very severe up to $100\%$ in a greenhouse. The causal fungus was identified as Pyricularia angulata Hashioka based on mycological characteristics and Path genicity of the fungus. The symptom of blast produced on leaves of banana was circular to oval, dark brow spots with yellow margins which measured 1-10mm in diameter, and the symptom on fruits was circular to oval reddish brown or dark brown, depressed spots which measured up to 20mm in diameter. The symptoms were scattered not only on leaves and fruits of banana but. also on petioles, leaf sheaths, bunch stalks and crowns The pathogen was characterized that conidia were hyaline, 2 septated, ovate to pyriform with a small hilum solitary at ends of den tides of conidiophores, and measured $16.0-34.0\times7.0-12.0{\mu}m$ (average $22.5\times9.0{\mu}m$) and conidiophores were mostly 2 septated, occasionally 2 or 3 branched, denticulate at the tips, and measured $7.5-100.0\times3.0-5.0{\mu}m$(average $90.0\times4.0{\mu}m$). The optimum temperature range for mycelial growth of the fungus on PDA was $26-28^{\circ}C$. The result of pathogenicity tests revealed that P. angulata had pathogenicity only on banana. On the other hand Pyricularia grisea (Cke.) Sacc. isolated from Digitaria sanguinalis(L.) Scopo. was not pathogenic to banana. It was revealed that the leaves were the most susceptible to P. angulata among several parts of banana in inoculation tests. Thirty-day-old fruits of banana were more susceptible to the fungus than 60-day-old fruits, and bunch stalks were more susceptible than the fruits.

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Incidence and Occurrence Pattern of Viruses in Lilies (Lilium spp.) on Jeju Island (제주지역 백합에서의 바이러스 발생 현황)

  • Kim, Hyo Jeong;Song, Jeong Heub;Song, MinA;Lee, Kwang Ju;Ko, Yoon Jeong;Park, Jeong Hoon;Yang, Young Taek;Heo, Tae Hyeon
    • Research in Plant Disease
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    • v.25 no.2
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    • pp.79-83
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    • 2019
  • To investigate the incidence status of lily viruses on Jeju island, lily samples were collected from 2015 to 2018 and examined for virus infection using RT-PCR. Of the viral infections, mixed and single infections were 70.0% and 17.9%, respectively. The incidence of mixed infections was highest for PlAMV and LSV as 43.4% in 2015; PlAMV, LSV 33.1% in 2016; LSV, LMoV 10.2% in 2017; and PlAMV, LSV, LMoV and CMV 15.8% in 2018. The incidence of PlAMV was observed to be 82.0% in 2015, 49.4% in 2016, 13.6% in 2017, and 39.5% in 2018 after the first occurrence of PlAMV in 2013. No symptoms were observed for single infection with LSV. However, in the case of mixed infection with LSV and LMoV, mosaic and leaf malformation symptoms appeared. With mixed infection with LSV and CMV, pale brown necrotic spots appeared, and mosaic and leaf curling were induced. PlAMV was more common in mixed infection than in single infection, and caused necrosis following the development of reddish-brown spots. PlAMV significantly decreased the marketability of lilies owing to the generation of leaf anomalies and curls, and its symptoms were more severe in mixed infections.

A study on the white porcelain in Sangju focus on the literature data (문헌자료를 중심으로 한 상주백자 연구)

  • Kim, Jong-Tae
    • Archives of design research
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    • v.19 no.5 s.67
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    • pp.17-30
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    • 2006
  • The early Joseon Dynasty is very important period of studying about Korean history of ceramics because various kinds of porcelain were produced like celadon, brown porcelain, white porcelain, inlaid white porcelain, blue porcelain etc, $\ulcorner$sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ was published and Saongwon established its branch. Moreover, there also had a sudden political change to Joseon Dynasty, so ceramic industry in Gangjin was closed and artisans in Gangjin scattered to find safer place. One of these place was Imiwueri and Chuhyunri, Joongmohyun in Sangju.(the name of the place in that times, now Modongmyun) Also, Sangju in Gyeongsangbuk-Do is one of the places where can get materials for producing white porcelain, so artisans in Gangjin could migrate to the place during change of regime to Joseon Dynasty which based on Sung Confucianism. In $\ulcorner$Tejongsilrok$\lrcorner$, it mentioned specific areas like Joongmo, Hwaryeong in Sangju, Gyeongsang-Do and we can find out about situations of ceramic production in the early 15 centuries with producing dishes for a Royal family. $\ulcorner$Sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ mentioned Imiwueri and Chuhyunri and Sangju produced the half of porcelains. In $\ulcorner$Gyeongsangdojiriji$\lrcorner$, Sangju takes charge of 8 spots which is one third of ceramic production. $\ulcorner$Gyeongsangdojiriji$\lrcorner$ and $\ulcorner$Sejongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$ were published with same goals at the same year. In $\ulcorner$Gyeongsangdosokchanjiriji$\lrcorner$, there was deleted nine spots which included high, middle, low level compared with $\ulcorner$Seiongsilrok$\lrcorner$ $\ulcorner$Jiriji$\lrcorner$, and in $\ulcorner$Donggukyeojiseungram$\lrcorner$ there was entirely deleted the locations of porcelain spots and potteries.

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