• Journal of Food Hygiene and Safety
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• v.16 no.3
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• pp.232-240
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• 2001

One hundred twenty five bacterial isolates were obtained from the brown blotch-diseased oyster mushrooms collected from markets. Among them, 45 were determined as pathogenic bacteria and white line forming organisms(WLFO) were 6 strains and white line reaction organisms (WLRO) were 6 strains. All of the white line forming isolates were identified as Pseudomonas tolaasii which is a known pathogen of brown blotch disease of oyster mushroom by GC-MIS(Gas chromatography-microbial identification system). Six of the white line reacting organisms were identified as P. chlomraphis, P. fluorescens biotype A and type C. The rest of them were P gingeri, P. agarici, P. fluorescens biotype B, P. chloroyaphis, non-pathogenic P. tolaasii, P. putida biotype A and B etc. For spectrum of activity of tolaasin, culture filtrates from pathogenic isolates were examined by browning of mushroom tissue and pitting of mushroom caps. The weak pathogenic bacteria didn't induce browning or pitting of mushroom tissue. On the other hand, strong pathogenic isolates showed browning and pitting reaction on mushroom. An extracellular toxin produced by P. tolaasii, was investigated. The hemolysis activity test of 6 strains identified as P. tolaasii were 0.8∼0.9 at 600 nm and 3 strains of WLRO were 0.9∼1.0 and Pseudomonas app. were 1.0∼1.2. Observation of fresh mushroom tissue using confocal laser scanning microscopy was carried out for images of optical sectioning and vertical sectioning. Also images of brown blotch diseased oyster mushroom tissue after contamination P. tolaasii was obtained by CLSM.

• Korean Journal of Environmental Biology
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• v.23 no.2 s.58
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• pp.157-162
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• 2005

To clarify the key characteristics of Korean brown frogs (R. dybowskii, R. huanrenesis and R. amurensis coreana), this study employed a morphological and morphometrical comparisons among brown frogs found in Korea and Japanese endemic species, R. tsushimensis. As a result, it was considered that the key characteristics were the shape of the finger and toe tips, the markings of the lower jaw, the presence or absence of an upper lip line, the development of toe webbing, and the shape of the vomerine teeth series by morphological comparisons of brown frogs. Also, there were significant differences in tympanum diameters and foot lengths of the four species of brown frogs. Therefore, a comparison between morphological and morphometric characters is an effective way to clarify the identification of brown frogs.

• Journal of Biosystems Engineering
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• v.22 no.3
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• pp.295-302
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• 1997

An ultimate purpose of this study was to develop an automatic system for brown rice quality inspection using image processing technique. In this study emphasis was put on developing an algorithm for discriminating the brown rice kernels depending on their external quality with a color image processing system equipped with an adaptor magnifying the input image and optical fiber for oblique lightening. Primarily, geometical and optical features of images were analyzed with paddy and the various brown rice kernel samples such as a sound, cracked, peen-transparent, green-opaque, colored, white-opaque and brokens. Secondary, geometrical and optical parameters significant for identifying each rice kernels were screened by a statistical analysis(STEPWISE and DISCRIM procedure, SAS wer. 6) and an algorithm fur on- line discrimination of the rice kernels in static state were developed, and finally its performance was evaluated. The results are summarized as follows. 1) It was ascertained that the cracked kernels can be detected when e incident angle of the oblique light is less than 2$0^{\circ}C$ but detectivity was significantly affected by the angle between the direction of the oblique light and the longitudinal axis of the rice kernel and also by the location of the embryo with respect to the oblique light. 2) The most significant Parameters which can discriminate brown rice kernels are area, length and R, B and r values among the several geometrical and optical parameters. 3) Discrimination accuracies of the algorithm were ranged from 90% to 96% for a sound, cracked, colored, broken and unhulled, about 81 % for green-transparent and white-opaque and 75 % for green-opaque, respectively.

• Asian-Australasian Journal of Animal Sciences
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• v.31 no.10
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• pp.1654-1659
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• 2018

Objective: This study was conducted to investigate the effects of dietary supplementation with fermented and non-fermented brown algae by-products on the laying performance, egg quality, relative organ weight, and blood profile of laying hens. Methods: Hy-Line Brown chickens (n = 180; 70-week-old) were randomly divided into 5 groups with 4 replicates per group (3 hens per cage, 4 cages per replicate), and fed with 5 experimental diets, namely the basal control diet (CON) or the control diet supplemented with 0.5% brown seaweed (BS), 0.5% seaweed fusiforme (SF), 0.5% fermented brown seaweed (FBS), or 0.5% fermented seaweed fusiforme (FSF), for 4 weeks. Results: Egg production rate and egg mass were greater in the BS group than in the other groups (p<0.05), and the SF and FSF groups had greater egg production than the control group (p<0.05). Egg weight was higher in the BS group than in the other groups (p<0.05). There were no differences in eggshell color, egg yolk color, eggshell strength, or eggshell thickness among the groups. There was no difference in Haugh units among the treatment groups, except for the FSF group, which had a significantly lower value (p<0.05). The non-fermented groups had greater relative organ weights, particularly the liver and cecum, than the other groups (p<0.05). Regarding blood profile, the supplemented-diet groups had higher albumin levels than the control group (p<0.05). The FBS group had higher total cholesterol and triglyceride levels than the other groups (p<0.05). The BS and FBS groups had higher glutamic pyruvic transaminase levels than the other groups (p<0.05). Conclusion: This study demonstrated that dietary brown algae supplementation can improve egg-laying performance; however, supplementation with fermented seaweeds had no positive effect on the egg-laying performance of hens.

• Korean Journal of Agricultural Science
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• v.46 no.3
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• pp.569-577
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• 2019

The current experiment was conducted to compare nutrient utilization in the aged laying hens with different eggshell strengths or different intensities of brown eggshell color. Two metabolic trials with 96-week-old Hy-Line Brown laying hens were conducted. Twelve hens with a strong eggshell strength (SES) and 12 hens with a weak eggshell strength (WES) were selected and used in the first experiment, whereas 12 hens with dark brown eggshells (DBE) and 12 hens with light brown eggshells (LBE) were selected and used in the second experiment. All hens were fed a common commercial layer diet. Diets included chromic oxide as an indigestible marker to determine the apparent total tract retention (ATTR) of the gross energy (GE), dry matter, acid-hydrolyzed ether extract, nitrogen, neutral detergent fiber, acid detergent fiber, ash, and minerals. The results indicate that the ATTR of Ca in the aged laying hens with SES (59.7%) was greater (p < 0.05) than in the aged laying hens with WES (48.6%). However, the ATTR of the GE and other nutrients was not different between the hens with SES or WES. There were no differences in the ATTR of GE and nutrients between the hens with DBE or LBE. In conclusion, aged laying hens with SES have a greater ability to utilize dietary Ca than those with WES, indicating that the extent of dietary Ca utilization determines the eggshell strength of aged laying hens. However, nutrient utilization in diets may have little impact on eggshell brown coloration in aged laying hens.

• Communications of the Korean Mathematical Society
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• v.19 no.3
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• pp.519-529
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• 2004

M. Brown [2] posed an open question on the class of free homeomorphisms as follows: if f is a free homeomorphism of two manifold and k is a positive integer then is $f^{k}$ free\ulcorner In this paper we show that the answer of the open question is true.

• Proceedings of the Korean Society of Life Science Conference
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• 2005.04a
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• pp.199-199
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• v.32 no.1
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• pp.49-57
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• 2019

Objective: The first stage in both breeding and programs for the conservation of genetic resources are the identification of genetic diversity in the relevant population. The aim of the present study is to identify genetic diversity of six brown layer pure chicken lines (Rhode Island Red [RIRI, RIRII], Barred Rock [BARI, BARII], Columbian Rock [COL], and line 54 [L-54]) with microsatellite markers. Furthermore, the study aims to employ its findings to discuss the possibilities for the conservation and sustainable use of these lines that have been bred as closed populations for a long time. Methods: In the present study, a total number of 180 samples belonging to RIRI (n = 30), RIRII (n = 30), BARI (n = 30), BARII (n = 30), L-54 (n = 30), and COL (n = 30) lines were genotyped using 22 microsatellite loci. Microsatellite markers are extremely useful tools in the identification of genetic diversity since they are distributed throughout the eukaryotic genome in multitudes, demonstrate co-dominant inheritance and they feature a high rate of polymorphism and repeatability. Results: In this study, we found all loci to be polymorphic and identified the average number of alleles per locus to be in the range between 4.41 (BARI) and 5.45 (RIRI); the observed heterozygosity to be in the range between 0.31 (RIRII) and 0.50 (BARII); and $F_{IS}$ (inbreeding coefficient) values in the range between 0.16 (L-54) and 0.46 (RIRII). The $F_{IS}$ values obtained in this context points out to a deviation from Hardy-Weinberg equilibrium due to heterozygote deficiency in six different populations. The Neighbour-Joining tree, Factorial Correspondence Analysis and STRUCTURE clustering analyzes showed that six brown layer lines were separated according to their genetic origins. Conclusion: The results obtained from the study indicate a medium level of genetic diversity, high level inbreeding in chicken lines and high level genetic differentiation between chicken lines.

• Korean Journal of Ichthyology
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• v.36 no.3
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• pp.263-272
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• 2024

The genus Rhinogobius fishes, collected from a stream in the Geumodo Island of Yeosusi, Jeollanam-do, Korea, were identified as Rhinogobius nagoyae Jordan and Seale, 1906. The specimen from Geumodo Island exhibited the following morphological characteristics, which were consistent with the original description of R. nagoyae and other literatures: I, 7~8 second dorsal fin rays; I, 7~8 anal fin rays; 18~19 pectoral fin rays; 31~36 longitudinal scales; 8~9 transverse scales; first dorsal fin elongate in male, its distal tip reaching to base of second or third branched ray of second dorsal fin in males when adpressed; pectoral fin base with 1~3 crescent-like reddish brown line; abdomen blue when mature; when alive or freshly-collected, cheek with vermiculate reddish brown line; caudal fin 6~7 distinct rows of dark dots; a bow-shaped dark brown blotches at caudal-fin base. Detailed specimen-based descriptions were reported here.

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.8
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• pp.1069-1074
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• 2014

In China and South East Asia, the duck (common duck) is important in egg production for human consumption. Plumage color is a breed characteristic and of economic importance, together with egg production. Our aim in this study was to investigate the inheritance of plumage color in three Chinese indigenous egg-type duck breeds, Shan Ma (S), Putian White (F) and Putian black (P), and some of their crossbreds. These three breeds have different plumage color and are used in crossbreeding. The crossbred laying ducks $F{\times}(P{\times}S)$ and $F{\times}(S{\times}P)$ showed highly improved laying ability but heterogeneous plumage color. Genotypes at four relevant loci were investigated by studying down color and pattern in ducklings after crossbreeding. $F_1$ ducklings from the matings $F{\times}S$ and $S{\times}F$, $P{\times}S$, and $S{\times}P$ were classified into four classes of plumage color (the Shan Ma plumage color, black, white, or multicolored) over three generations. Parents were selected for the Shan Ma plumage color of their progeny. In the fourth generation, P male and P female ducks were selected according to the frequency of the desired class of plumage color (Shan Ma) of their $F_1$ progeny to obtain the so-called "Brown Putian Ma duck". The Shan Ma duck genotype was identified as having the restricted mallard color pattern ($M^RM^R$), full expression of any of the patterns or colors (CC), no extended black (ee) and no brown dilution D (D). The Putian White genotype was recessive white (cc), no extended black (ee) and no brown dilution D (D). The Putian Black genotype exhibited full expression of extended black (E gene) and no brown dilution (CCEE D [D]). It was shown that $F{\times}S$ and $S{\times}F$ tests should be implemented to eliminate the recessive white c allele in the S line and the dominant extended black E allele in the F line. It was also shown that the Brown Putian Ma obtained from Putian Black, with no extended black genotype (ee), could be used to get rid of the black plumage (E gene) in the crossbred ducks. This could provide a solution for producing 3-way crossbred ducks Putian $White{\times}$(Putian-$Ma{\times}Shan$ Ma) and Putian $White{\times}$(Shan $Ma{\times}Putian$-Ma), with the desired Shan Ma feather color.