• Korean Journal of Poultry Science
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• v.33 no.2
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• pp.127-131
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• 2006

This study was conducted to investigate the effects of expanding feed processing technologies on the egg characteristics and nutrient digestibility in laying hens. A total of two hundred seventy laying hens were randomly allocated into three treatments with fifteen replications for six weeks. Dietary treatments include 1) MS(mash), 2) EC(expanded crumble) and 3) EP(expanded pellet). In the egg production, the EC and EP treatments were significantly higher than MS treatment(p<0.05). However, no significant difference was founded in egg weight(p>0.05). Egg shell breaking was increased in EC and EP treatments compared with MS treatment(p<0.05), however, egg shall thickness showed no significant difference(p>0.05). Egg production and egg shell breaking were significantly higher in EC and EP treatments than MS treatment(p<0.05), but egg weight and egg shell thickness were no significantly difference among treatment(p<0.05). Yolk color was increased in EC treatment compared with other treatments(p<0.05). Also, yolk index was significantly improved in EC and EP treatments compared with MS treatment(p<0.05). Haugh unit was increased in EC treatment compared with MS treatment(p<0.05). EC treatment had improved DM, Ca and P digestibility. In conclusion, Expanded crumble and pellet processing could improve the egg characteristic and nutrient digestibility in laying hens.

• Proceedings of the Korea Society of Poultry Science Conference
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• 2003.11a
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• pp.9-27
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• 2003

This study was performed to measure the effect of carotenoid polarity on absorption and Pigmentation in blood, muscle, and skin of laying hens. Carotenoids used in this study and Polarity were ${\beta}$-8-Apo-carotenoic acid ethyl ester(ACAEE) ＞ astaxanthin ＞ canthaxanthin ＞ ${\beta}$-carotene. The chickens used in this study were 61∼78 weeks old ISA brown laying hens. Experiment #1 was designed to measure the effect of carotenoid level on the accumulation of carotenoids in carcass of laying hens after feeding for 6 weeks. D-carotene was accumulated in skin only at a detectable level when it was fed at 300 mg/kg feed. The skin was pigmented as yellow when it was measured by colorimeter. The concentration of ${\beta}$-carotene in blood was proportional to that in the feed. Pigmentation of muscle by 9-carotene was not effective. Canthaxanthin significantly increased redness of the skin(p＜0.05). However, canthaxanthin did not pigment muscle. The level of canthaxanthin in the blood and skin increased as the concentration in feed increased. ACAEE at 200 and 300 mg/kg feed significantly increased yellowness of the skin(p＜0.05). At all levels of ACAEE used($\geq$50 mg/kg feed) the b values of colorimeter increased. With increases in the contents of ACAEE, the concentration of ACAEE in the blood and skin increased. Compared to ${\beta}$-carotene, ACAEE and canthaxanthin were absorbed 9- and 3-fold more into the blood, respectively. The concentration of ACAEE and canthaxanthin in the skin was 1/10 of those in the blood. The lower were the concentrations of carotenoids in the feed, the higher were the absorption rates(from feed to blood and from blood to skin) The results indicated that the higher was the polarity of carotenoids, the more effective were the absorption and pigmentation. In experiment #2, the effect of carotenoid levels of feed on the accumulation of carotenoids in each body part of laying hens was determined. The colorimeter values for redness and yellowness significantly increased when canthaxanthin was fed at $\geq$50 mg/kg feed(p＜0.05). Breast and thigh were not affected by feeding of canthaxanthin at the levels used. The L values of muscle but not the a and b values were significantly affected by feeding at $\geq$200 mg/kg feed for wings and breasts, respectively. The yellowness of skin and muscle significantly increased when ACAEE was fed at $\geq$ 100 and $\geq$ 200 mg/kg feed, respectively(p＜0.05).

• Korean Journal of Poultry Science
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• v.32 no.4
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• pp.269-273
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• 2005

The wild ginseng culture by-products(WGCB) was added to the laying hens diets. A total of 420 Lohmann Brown laying hens were alloted to five levels of drinking water containing WGCB 0, 0.4, 0.8 1.6, $3.2\%$with seven replications and fed com-soybean meal based diets contained ME 2,800 kcal/kg, CP $16\%$. Laying Performance, egg quality, egg yolk fatty acid and cholesterol concentration were measured. Egg Production and daily e99 mass were significantly lower(p<0.05) in birds fed the WGCB $3.2\%$ compared with no WGCB group, but showed no significant effect in other treatments. Feed efficiency of birds fed $0.4\%$ WGCB showed the lowest of all treatments(p<0.05). Eggshell thickness was increased(p<0.05) by WGCB supplementation, whereas eggshell breaking strength, haugh unit and yolk color index were not affected. No significant change in yolk fatty acids and cholesterol were observed by the addition of WGCB.

• Journal of Animal Environmental Science
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• v.15 no.3
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• pp.199-208
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• 2009

This study was conducted to investigate the effects of dietary silicate minerals on ammonia emission from the excreta and performance in laying hens. A total of one thousand and twenty 49 week-old Lohmann brown layers were divided into three treatment groups consisting two replicates of 170 birds each. The experimental diets were containing three kinds of silicate minerals (Sand, Na-bentonite and SY feldspar) added at the level of 2% by top-dressing. Ammonia emission from the excreta was significantly decreased (p<0.05) by the supplementation of Na-bentonite or SY feldspar. N concentration of the excreta was significantly higher (p<0.05) for the hens fed Na-bentonite or SY feldspar compared to those fed sand. However, no significant differences were observed in feed intake and laying performance with over 80% laying rate observed for all the treatments. Egg shell strength was diminished when SY feldspar was added to the diet. As a whole, the results obtained from the experiment indicated that supplementation of Na-bentonite or SY feldspar added at 2% to the laying hen diet should not affect laying performance but help to reduce ammonia from the excreta at the same time.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.8
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• pp.1043-1048
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• 2010

This study investigated the effects of Fe-soy proteinate (Fe-SP) and magnesium oxide (MgO) dietary supplements on eggshell quality in laying hens. A total of 800 26-wk-old Hy-Line Brown hens were assigned to four dietary treatments: control (C), Fe-SP 100 (100 ppm Fe-soy proteinate), MgO (3 g MgO/kg diet), and Fe-SP 100+MgO. Each treatment had five replicates of 40 hens. The FT-IR (Fourier transform infrared) and XRD (X-ray diffraction) spectra of Fe-soy proteinate were different from those of $FeSO_4$ and soy digest. There were no significant differences among treatments in hen-day egg production, hen-house egg production, broken and soft egg rate, feed intake, or feed conversion. The MgO and Fe-SP 100+MgO treatments showed significantly (p<0.05) higher egg weights than the control group. Eggshell strength and thickness were significantly (p<0.05) higher in the MgO supplemented groups. The lightness and yellowness of the eggshells decreased, and the redness increased significantly in the Fe-SP treated groups. There were no significant differences among treatments in leukocyte level, but hemoglobin (Hb) concentrations were higher with Fe-SP treatments. In conclusion, supplementation with Fe-SP significantly affects eggshell color and hemoglobin concentration, whereas MgO supplementation increases eggshell strength and thickness. Egg weight and egg shell quality can be improved by supplementation of 100 ppm Fe in the form of Fe-SP and 3 g MgO/kg diet.

• Korean Journal of Poultry Science
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• v.27 no.3
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• pp.221-226
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• 2000

This experiment was conducted to determine the effect of replacing limestone by sea shell on digestibility, egg strength and thickness, Ca and P of serum and Ca and P of tibia in layer hens. A total 54 brown layers was assigned to the treatments. There were six layers per replicate with three replicate per treatment. Treatments were layer diet with 7.5% limestone as control, 7.0% limestone and 0.5% sea shell in diet(SS0.5) and 6.5% limestone and 1.0% sea shell in diet(SS1.0). Digestibility of DM was significantly difference between SS0.5 and SS1.0(P〈0.05). Digestibilities of Ca and P were not affected by treatments(P〉0.05), however, SS1.0 tended to increase Ca and P digestibility compared to control. Egg thickness and egg strength were no differences among the treatments(P〉0.05). Differences of initial and finial period on Ca of plasma were significantly affected in the a.m. between SS0.5 and SS1.0(P〈0.05). However, there was no difference in Ca of plasma in the p.m. among the treatments and Ca of plasma in control had a tendency to decrease in the p.m. compared to that of treatments(P〉0.05). Ca of tibia was significantly increased by SS1.0 compared to control(P〈0.05). However, there was no difference in P of tibia among the treatments(P〉0.05). Based on these results, dietary supplemental sea shell mat have a role to improve the egg quality of layer hens.

• Food Science of Animal Resources
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• v.30 no.3
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• pp.449-457
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• 2010

This study investigated the effects of three kinds of water extract mixtures each with different ratios of A. capillaris, C. sinensis, S. chinensis, and V. coloratum on laying performance, egg quality, blood characteristics, and egg storage stability in laying hens. One hundred and sixty 35-wk-old laying hens (Hy-line brown) were divided into four groups of 10 birds each replicated times four. The treatments involved a basal diet (C, control) or diets containing three kinds of water extract mixtures at 200 ppm (T1, water extract mixture A; T2, water extract mixture B; T3, water extract mixture C). The egg production rate, egg mass, and feed conversion ratio were significantly improved in all treatments compared to those of control (p<0.05). Haugh unit and egg yolk color in all treated groups were significantly improved in comparison with control (p<0.05), but no significant differences were observed for egg shell quality. The content of total cholesterol in blood serum was significantly decreased in all treatments compared to that of control (p<0.05). The blood biochemical parameters of liver and kidney damage were significantly decreased in all treatments compared to those of control (p<0.05). Total antioxidant activity in the blood serum of all treated groups was increased as well. The Haugh unit and malondialdehyde content in the egg yolk during storage were significantly decreased in all treated groups compared to control (p<0.05). Consequently, the dietary supplementation of water extract mixtures of A. capillaris, C. sinensis, S. chinensis, and V. coloratum improved laying performance and egg quality but decreased egg quality deterioration with increased storage. These results suggest that medicinal plant extracts could be used as a feed additive in feed for laying hens.

• Korean Journal of Poultry Science
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• v.22 no.4
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• pp.193-202
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• 1995

This study was carried out to investigate the effects of diverse dietary Ca levels and to determine whether bone mineral metabolism is influenced by the arnount of dietary Ca when given a choice of diets containing various levels of Ca. Forty Hy-line brown laying hens housed in separate cages were fed self-selected Ca diets. Birds were allowed a 7-day adaptation period followed by an 8-day collection period. Feed and water were available for ad libitum consumption. Eggs and excreta were collected at 6-h intervals during the day for mineral analysis. The Ca contents in excreta and retained Ca in the body on egg forming day were proportional to the amount of daily Ca intake. The retained Ca in the body were 0.97 g in control and 1.24~1.74 g in self-selected groups, respectively. Daily Ca contents (%) in tibial cortex were not consistent with feeding time intervals. The Ca content in tibial medulla in control group was lower than those of self-selected feeding groups(P<.05). The medullary Ca content in all treatment groups increased from 10:00 to 16:00 in a day. Ca content in plasma was low between 10:00 and 16:00 and was high between 22:00 and 04:00 in the following day.

• Korean Journal of Poultry Science
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• v.31 no.1
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• pp.9-15
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• 2004

This study was conducted to evaluate the effects of dietary herbal plant mixture on the performance in laying hens under heat stress. One hundred ninety two 54-weeks-old ISA Brown commercial layers, were used in 56 d experimental assay. Dietary treatments included CON (control; basal diet), HPM0.05 (basal diet ＋ 0.05% herbal plant mixture), HPM0.1 (basal diet ＋ 0.1% herbal plant mixture), and HPM0.2 (basal diet ＋ 0.2% herbal plant mixture). For overall period, the hens fed with HPM0.1 and HPM0.2 diets showed lower in the hen day egg production than the hens fed with CON diet(P＜0.05). At the end of the experimental period, egg weight was heavier in HPM 0.1 treatment than in CON (P＜0.05). There were no significant differences among the treatments in egg shell breaking strength, egg shell thickness, Haugh unit, and yolk color unit. Total cholesterol concentration of yolk tended to decrease as the level of herbal plant mixture in the diet increased. Total protein of blood was higher in the hens fed with herbal plant mixture than in the hens fed with CON diet (P＜0.05). Albumin concentration of blood was increased in HPM0.05 and HPM0.1 treatments compared with CON(P＜0.05). Red blood cell (RBC) and white blood cell (WBC) concentrations in serum were increased in HPM0.1 and HPM0.2 treatments compared with CON treatment (P＜0.05). In conclusion, dietary herbal plant mixture in laying hens under heat stress adversely affected egg production but increased total protein, albumin, RBC and WBC in blood.

• Korean Journal of Poultry Science
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• v.29 no.2
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• pp.81-88
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• 2002

The objective of this study was to investigate the line-by-feed additive interaction on egg traits and yolk cholesterol level in laying hens. Hy-line W-98 (white shell line) and Hy-line Brown (brown shell line) were used as experimental stocks and three groups were assigned in each line. The age of hens used in this study were from 40- to 46-wk-old. Two different levels of chitosan were also used as additive in chicken diet. The first (CH$_{30}$) and second (CH$_{60}$) experimental groups were fed basal diet added 30mg and 60mg chitosan/kg feed, respectively. And the control group (CON) hens were fed with basal diet only. The eggs were collected and analyzed at the end of 40, 42, 44, and 46-wk-old. In the mean egg weight, the CH$_{30}$ in Hy-line Brown was significantly heavier (P<0.05) than CON, but the CH$_{30}$ in Hy-line W-98 was significantly lighter (P<0.05) than CON. Whereas, the mean egg weight did not significantly differ between CH$_{60}$ and CON in both line. The mean albumen percentage in Hy-line Brown did not show significant between experimental groups and control group. On the other hand, the mean albumen percentage of CH$_{60}$ in Hy-line W-98 was significantly lower (P < 0.05) than that of CON, but there was no significantly different between CH$_{30}$ and CON. In the mean yolk percentage, it showed a reverse trend as shown in the mean albumen percentage; there were significantly higher (P<0.05) in CH$_{60}$ than in CON fur the Hy-line W-98, and higher in CH$_{30}$ than in CON for the Hy-line Brown. However, the mean shell percentages did not significantly differ between experimental groups and control group in both lines. In the mean yolk cholesterol levels, the CH$_{30}$ and CH$_{60}$ in both lines were lower by 0.46 mg ∼2.80 mg per gram yolk than CON. Although the interaction effect of line-by-supplementing level of chitosan in hens diet on the pooled mean of yolk cholesterol level was no significant, it showed a highly significant interaction (P<0.01) at three of four experimental ages.