• 농업생명과학연구
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• 제43권3호
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• pp.1-6
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• 2009

빗자루병에 의하여 심각하게 이병된 제주도 5.16도로의 왕벚나무 가로수들에 대하여 피해 상황과 이병 발병 원인을 생태적으로 밝히고 그에 따른 방제 대책을 마련키 위하여 본 연구를 수행하였다. 왕벚나무 가로수 중 45년생 이상의 노거수들은 거의 모두가 심각한 정도의 빗자루병에 감염되었으며, 30년생 역시 모든 나무들이 감염되었으나 중간 정도의 발병 반응을 보이고 있었다. 그러나 수령이 23년생 미만의 왕벚나무들은 병징을 찾아볼 수 없는 건전주로 생장하고 있었다. 3년 주기로 전정 작업을 실시한 제주대학교 입구에 식재된 왕벚나무에서는 도로변 남쪽면의 나무들이 북쪽면에 비해 병 발생이 심하고 생육 또한 저조하였다. 병 발생이 심각한 산천단으로부터 견월악까지의 횡단로 좌우 인근 30m내에 자생하는 벚나무들은 수령에 따라 차이는 있지만 병 발생이 경미하였다.

• Journal of Photoscience
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• 제9권2호
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• pp.338-340
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• 2002

In broom sorghum, Sorghum bicolor Moench, UV causes anthocyanin synthesis having action peaks in UVA and UVB regions. We previously reported that UV induces anthocyanin synthesis through UVB photoreceptor and phytochrome activated by UV. Furthermore, UVA and UVB amplify phytochrome-induced anthocyanin synthesis (PIAS). Our action- spectroscopic research indicated that a UV -receptor for amplification of PIAS is likely to be the same or same type of UVB photoreceptor for induction of anthocyanin synthesis. UVA-amplification of PIAS can be explained by the action of a cryptic red light signal (CRS), an amplification factor for PIAS produced by a distinct phytochrome-species activated by UVA. We suggest that UVA photoreceptors are not involved in anthocyanin synthesis in the broom sorghum.

• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.73.2-73
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• 2003

Typical whiches broom symptoms caused by phytoplasma were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms were showing abnormally small leaves, short internodes, and proliferation of shoots. Fluorescence and electron microscopy of leaf midribs revealed phytoplasma positive DAPI fluorescence and numerous phytoplasma bodies localized in the phloem sieve tubes. Phytoplasma DNA of 1.8 Kb was detected consistently from all symptomatic samples by the amplification of phytoplasma DNA with the phytoplasma specific primer pair Pl/P7. But no phytoplasma DNA was detected in healthy ash seedlings. Based on sequence analyses of an amplified region, this phytoplasma is closely related to Eqilodium phyllody, Mulberry dwarf, and Aster yellows phytoplasmas with the homology of 99.95 ％, 99.79 ％ and 99.78 ％, respectively, This phylogenetic analyses indicate that ash witches broom phytoplasma but is evidently distinct from the ash yellows group 16SrⅦ and should be classified into the Aster yellows group 16SrⅥ.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2003년도 Proceedings of ACRS 2003 ISRS
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• pp.694-696
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• 2003

The KOMPSAT/OSMI remote sending data of 800 km swath are collected by whisk broom method employing 96 charge coupled devices (CCDs). The stripping noise in the OSMI imageries, which arise mainly due to the non-uniform sensitivities of 96 CCDs, are the major hindrance for oceanographic applications of the OSMI data. The OSMI images are corrected by 'Ensemble Smoothness' method which is based on an assumption that the series of the averages and variances of digital numbers in each line should vary smoothly. The data of each line are corrected by linear regression model of which coefficients are obtained by Ensemble Smoothness method. Our algorithm can be applied not only to OSMI data but also for other remote sensing date collected by whisk broom or push broom.

• 한국산림과학회지
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• 제94권2호통권159호
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• pp.103-107
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• 2005

Typical phytoplasma whiches' broom symptoms were observed in Ash (Fraxinus rhynchophylla Hence) in Korea. The symptoms of the disease were showing abnormally small leaves, shorted internodes and proliferation of shoots. Examination of fluorescent and electron microscopy of leaf midribs revealed numerous phytoplasma bodies localized in the phloem tube cells. The phytoplasmas were detected in all the symptomatic samples by the amplification with phytoplasma specific primer pair P1/P7 consistently, and the expected size was 1.8 kb. However, the phytoplasma DNA was not detected in healthy seedlings. Based on sequence analysis of amplified region, this phytoplasma has close homologies with eqilodium phyllody, mulberry dwarf, and aster yellow phytoplasmas, 99.95%, 99.79% and 99.78%, respectively, This phylogetic analysis indicates that ash witches' broom phytoplasma should be classified in the aster yellow group 16SrVI and clearly distinct from the ash yellow group 16SrVII.

• 한국식물병리학회지
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• 제14권5호
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• pp.382-385
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• 1998

Molecular identification and genetic relationships between a phytoplasma associated with chestnut little leaf (CLL) and phytoplasma isolates of other trees in Korea were amplified by polymerase chain reaction (PCR). These 16S rDNA sequences amplified from the various phytoplasmas were used in DNA heteroduplex mobility assays (HMA). In DNA HMA combined with PCR, the mobility shift was observed for a heteroduoplex formed in combined with CLL and jujube witches broom, but not for those formed in combined with CLL and each of sumac witches broom, paulownia witches broom, and mulberry dwarf. HMA combined with PCR has been shown to be a very useful method for detection and differentiation of phytoplasmas.

• 한국군사과학기술학회지
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• 제22권4호
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• pp.484-491
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• 2019

General Hyperspectral Image Sensor acquires an image of line form such as a thin rectangle shape because of using 1D array Push Broom or Whisk Broom scanning method. A special mechanism is required for a Hyperspectral Image Sensor to operate for nadir and slant range. To design the mechanism, the characteristics of the flight motion and the overlap rate between consecutive frames were analyzed. Also, system requirements were proposed through modeling and simulation.

• Korean Journal of Geomatics
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• 제4권1호
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• pp.31-37
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• 2004

The aim of this research is comparing the existing approximation models (e.g. Affine Transformation and Direct Linear Transformation) with Rational Function Model as a substitute of rigorous sensor model of linear array scanner, especially push-broom sensor. To do so, this research investigates the mathematical model of each approximation method. This is followed by the assessments of accuracy of transformation from object space to image space by using simulated data generated by collinearity equations which incorporate or depict the physical aspects of linear array sensor.

• Journal of Microbiology and Biotechnology
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• 제23권8호
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• pp.1047-1054
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• 2013

Witches' broom of lime is a disease caused by Candidatus Phytoplasma aurantifolia, which represents the most significant global threat to the production of lime trees (Citrus aurantifolia). Conventional disease management strategies have shown little success, and new approaches based on genetic engineering need to be considered. The expression of recombinant antibodies and fragments thereof in plant cells is a powerful approach that can be used to suppress plant pathogens. We have developed a single-chain variable fragment antibody (scFvIMP6) against the immunodominant membrane protein (IMP) of witches' broom phytoplasma and expressed it in different plant cell compartments. We isolated scFvIMP6 from a naïve scFv phage display library and expressed it in bacteria to demonstrate its binding activity against both recombinant IMP and intact phytoplasma cells. The expression of scFvIMP6 in plants was evaluated by transferring the scFvIMP6 cDNA to plant expression vectors featuring constitutive or phloem specific promoters in cassettes with or without secretion signals, therefore causing the protein to accumulate either in the cytosol or apoplast. All constructs were transiently expressed in Nicotiana benthamiana by agroinfiltration, and antibodies of the anticipated size were detected by immunoblotting. Plant-derived scFvIMP6 was purified by affinity chromatography, and specific binding to recombinant IMP was demonstrated by enzyme-linked immunosorbent assay. Our results indicate that scFvIMP6 binds with high activity and can be used for the detection of Ca. Phytoplasma aurantifolia and is also a suitable candidate for stable expression in lime trees to suppress witches' broom of lime.

• 한국산림과학회지
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• 제86권1호
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• pp.1-8
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• 1997

뉴질랜드 북섬의 다우지역에서 라디아타소나무 유묘생장이 일련의 주요 잡초종과의 경쟁으로 인하여 감소함을 계량화(計量化)하기 위하여 계획된 실험의 2차년도 결과이다. 라디아타소나무 유묘(1/0)들을 각각 잡초제거구와 활엽성초본, 목초, broom, pampas, buddleia, gorse와 함께 생육시켰다. 양료와 수분공급은 각 요인에 관수와 시비, 무관수와 무시비로 다양화하였다. 식재 21개월 후 라디아타소나무 유묘의 재적생장량은 잡초제거구와 gorse와 함께 생육된 구에서 최대였고 buddleia나 pampas와 함께 생육된 곳에서는 최소였다. 유묘생장에 대한 잡초의 영향이 수분이나 양료 경쟁에 의해 조정된다는 증거가 없었다. 유묘를 능가하여 치솟는 키크고 빨리 자라는 종(broom, buddleia, pampas)들이 유묘 생장에 가장 큰 영향력을 갖고 있고 그 영향력의 크기는 유묘 위에 뒤덮는 정도와 상관관계가 있었다. 이것은 피음 혹은 광경쟁이 아마도 중요한 인자임을 암시해 주고 있다.