• 식물병과 농업
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• 제1권1호
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• pp.30-36
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• 1995

Infection rate of witches'-broom disease (WB) was observed yearly in transplanted jujube seedlings for 7 years. Seedlings of‘Hongan’grafted either on the rootstock from seed (RS) or on the rootstock from rooting (RR) were planted in separate farms. Occurrence of the disease was determined 4 years after transplanting with the witches'-broom symptom. Only 1 seedling of RS was infected with WB, while 56 seedlings of RR showed WB. Yearly infection rates of WB were observed in several varieties of jujube including‘Boeun’,‘Keumsung’,‘Moodeung’,‘Bokjo’,‘Koori’(10 trees for each in Cheongjoo), and‘Hongan’(165 trees in Boeun). In most varieties, WB first appeared in the third year from transplanting, and in the seventh year, more than 80% of the total tree showed WB. The yearly infection rate of newly infected trees was almost. stable during the first years. However, the ratio sharply increased from the sixth year. The accumulated infection rate of WB increased double, year by year from the third to seventh year. Between Cheongjoo and Boeun, no difference in infection rates was found.

• 한국식물병리학회지
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• 제10권3호
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• pp.211-214
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• 1994

Paulowina witches'-broom mycoplasma-like organism (PWB-MLO) was transmitted experimentally to periwinkle (Catharanthus roseus L.) plant by tobacco leaf bug (Cyrtopeltis tenuis Reuter). Adults of the leaf bugs were allowed to feed on the witch's-broom infected paulownia (Paulownia tomentoas Steud.) trees for three weeks to insure the acquisition of PWB-MLO and then transferred to healthy seedlings of periwinkle and paulownia plants. In 25∼35 days after transfer of the viruliferous leaf bugs, six out of the ten periwinkle plants showed‘little-leaf’symptoms, while the paulowina seedlings remained symptomless. Presence of MLO in the infected periwinkle tissue was diagnosed by fluorescence microscopy and MLO particles were observed under electron microscope, confirming the transmission of PWB-MLO to periwinkle.

• The Plant Pathology Journal
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• 제18권6호
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• pp.308-312
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• 2002

Heteroduplex mobility assay (HMA) and single-strand conformation polymorphism (SSCP) analyses combined with PCR were developed for genetic differentiation of various phytoplasma isolates. In the HMA and SSCP analyses, differences in the mobility shifts and the SSCP band patterns identified three distinct types of phyto-plasmas: Type Ⅰ, jujube witches'-broom (JWB) and ligustrum witches'-broom (LiWB); Type Ⅱ, mulberry dwarf（MD) and sumac witches'-broom (SuWB); and Type Ⅲ, paulownia witches'-broom (PaWB). Results of the sequence analyses revealed that phytoplasmas of JWB and MD had 100％ homology with LiWB and SuWB, respectively. On the other hand, PaWB phyto-plasma had 97.8% homology with MD phytoplasma. The PCR-HMA and SSCP techniques were very useful in determining variations in sequence among several isolates of phytoplasmas. Furthermore, the methods were rapid, economical, highly sensitive, and easy to handle with the gels.

• 식물병연구
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• 제17권3호
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• pp.265-271
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• 2011

우리나라에서 화훼류에 7종류의 파이토플라스마병이 발생하였다. 국화의 Ph-ch1과 Ph-ch2, 나리의 Ph-lily, 페튜니아의 petunia flat stem(PFS-K), 포인세티아의 poinsettia branch inducing(PoiBI-K), 스타티스의 statis witches' broom (SWB-K)과 아잘레아의 azalea witches broom(AWB) 등이다. 16S rRNA 유전자 염기서열을 기본으로 화훼류 파이토플라스마를 분류한 결과 우리나라에는 aster yellow(AY), stolbur와 X-disease 순으로 많이 발생하였다. 파이토플라스마의 특징적인 병징 가운데 하나인 대화증상은 단자엽 식물인 나리와 페튜니아, 포인세티아와 같은 쌍자엽식물에서 모두 발생하였다. 또한 대화증상은 stolbur 그룹의 Ph-lily, AY 그룹의 petunia PFS-K와 X-disease의 포인세티아 PoiBI-K에서 모두 나타났다. 이 결과는 16S rRNA 유전자 염기서열에 기초를 둔 파이토플라스마 분류와 증상과는 일관성있게 일치하지 않는다는 것을 알 수 있다. 우리나라 화훼류에서 발생한 7종의 파이토플라스마를 대추나무빗자루, 오동나무빗자루, 묏대추나무빗자루, 뽕나무 오갈 및 모감주나무파이토플라스마 등 5종의 수목 파이 토플라스마와 16S rRNA 유전자의 염기서열을 비교한 결과 88.5-99.9%의 매우 높은 상동성을 나타내었다. 특히 뽕나무오갈병 파이토플라스마는 PoiBI-K를 제외한 6종의 화훼류 파이토플라스마와 96.3-99.9% 가장 높은 상동성을 나타내었다. 이 결과로 우리나라 화훼류에 발생한 파이토플라스마병은 매개충을 통하여 수목으로부터 전염되었을 것으로 추정되었다.

• 한국응용곤충학회지
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• 제5_6권
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• pp.1-7
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• 1968

본 연구는 오동나무천구소병 바이러스의 매개충을 구명하기 위하여 착수했으며, 결과는 다음과 같다. 1) 오동나무천구소병의 이병수에 모여드는 흡수성곤충 중에서 가장 발생이 많았던 곤충은 담배장님노린제며 난, 약충, 성충이 8월 중순부터 10월 하순까지 발생하였다. 2) 담배장님노린재가 오동나무천구소병 바이러스의 매개곤충임이 구명되었다. 3) 담배장님노린재에 의하여 오동나무천구소병 바이러스를 접종한 결과 오동나무유식물에서는 접종 후 20일만에 발병을 보았고 접종 40일만에 오통나무천구소병 특유의 병징이 발현되었다. 4) 접종실험을 통해서 확인된 오동나무천구소병 바이러스의 기주식물은 오동나무 이외에 금잔화 및 나팔꽃이며, 일년감$\cdot$당근$\cdot$과꽃$\cdot$ 백일흥$\cdot$코스므스$\cdot$대두$\cdot$질경이 등에서는 충접종 후 2개월까지도 뚜렷한 발병을 확인할 수 없었다. 5) 금잔화와 나팔꽃은 발병까지의 잠복기간이 짧고 병징발현이 뚜렷한 점으로 미뤄 오동나무천구소병 바이러스의 유망한 검정식물로 생각된다.

• 대한원격탐사학회:학술대회논문집
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• 대한원격탐사학회 2002년도 Proceedings of International Symposium on Remote Sensing
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• pp.400-406
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• 2002

Recently, the enormous increase in the volume of remotely sensed data is being acquired by an ever-growing number of earth observation satellites. The combining of diversely sourced imagery together is an important requirement in many applications such as data fusion, city modeling and object recognition. Aerial triangulation is a procedure to reconstruct object space from imagery. However, since the different kinds of imagery have their own sensor model, characteristics, and resolution, the previous approach in aerial triangulation (or georeferencing) is performed on a sensor model separately. This study evaluated the advantages of aerial triangulation of large number of images from multi-sensors simultaneously. The incorporated multi-sensors are frame, push broom, and whisky broom cameras. The limits and problems of push-broom or whisky broom sensor models can be compensated by combined triangulation with frame imagery and vise versa. The reconstructed object space from multi-sensor triangulation is more accurate than that from a single model. Experiments conducted in this study show the more accurately reconstructed object space from multi-sensor triangulation.

• 미생물과산업
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• 제25권2호
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• pp.11-18
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• 1999

전북지방에 만연되고 있는 고구마 위축병에 관한 병원성 검정에 따른 결과는 다음과 같다. 1. 본고구마 위축병의 병원은 mycoplasma-like organism(또는 PPLO)으로서 그 형태는 타원형이며 크기는 200~2500범위이다. 2.항생물질 효과는 oxytetracycline이 가장 효과적이다. 3.전자현미경 관찰 결과 mycoplasma-like bodies 는 엽맥 관세포 속에 밀집되여 있고, 항생물질에 의해 모구조의 파괴현상이 나타난다. 4. 감염경로는 접목전염만이 되고, 즙액전염, 곤충전염에서는 감염이 되지 않았다.

• 한국산림과학회지
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• 제67권1호
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• pp.28-30
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• 1984

형광색소(蛍光色素) DAPI(4'-6-diamidino-2-phenylindole, 2HCI)와 형광원미경(蛍光願微鏡)을 이용(利用)한 조직화학적(組織化學的) 기법(技法)으로 빗자루병(病)에 걸린 대추나무 조직내(組織內)의 마이코플라스마 분포(分布)를 조사(調査)한 결과(結果), 빗자루병징(病徵)이 나타나 있는 가지에서는 외관상(外觀上) 건전엽(健全葉)을 포함(包含)하여 모든 잎과 줄기에서 마이코 플라스마가 검출(檢出)되었으나 병징(病徵)이 나타나 있지 않은 가지의 잎과 줄기에서는 마이코플라스마가 검출(檢出)되지 않았다. 또한 감염(感染)된 나무의 뿌리조직(組織)에서도 뚜렷한 형광반응(蛍光反應)이 나타남으로써 마이코플라스마가 뿌리조직(組織)에도 많이 존재하고 있음을 알 수 있다.

• The Plant Pathology Journal
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• 제21권4호
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• pp.383-386
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• 2005

In 2003 typical phytoplasma symptoms of witches' broom and flower malformation were observed on statice (Limonium sinuatum) plants grown at commercial greenhouses in Busan, South Korea. The DNA extracted from the infected leaves was amplified using universal primer pair of Pl/P6 derived from conserved 16S rRNA gene of Mollicutes giving the expected Polymerase chain reaction (PCR) product of 1.5 kb. In the nested PCR assays, the expected DNA fragment of 1.1 kb was amplified with the specific primer pair 16Fl/Rl that was designed on the basis of aster yellows (AY) phytoplasma 16S rDNA sequences. The 1.1 kb PCR products were cloned and nucleotide sequences were determined. The sequences were identical to that of Onion yellows OY phytoplasma (GenBank accession no. D12569) isolated from Onion in Japan. Electron microscopy of thin sections of leaf veins showed phytoplasma bodies in the phloem. Statice witches' broom symptom occurred on statice in commercial greenhouses in Korea was confirmed as infection of AY phytoplasma by transmission electron microscopy observation, and by determination of 16S rRNA gene sequences of phytoplasma.

• 대한원격탐사학회지
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• 제19권3호
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• pp.255-261
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• 2003

Recently, the enormous increase in the volume of remotely sensed data is being acquired by an ever-growing number of earth observation satellites. The combining of diversely sourced imagery together is an important requirement in many applications such as data fusion, city modeling and object recognition. Aerial triangulation is a procedure to reconstruct object space from imagery. However, since the different kinds of imagery have their own sensor model, characteristics, and resolution, the previous approach in aerial triangulation (or georeferencing) is purformed on a sensor model separately. This study evaluated the advantages of aerial triangulation of large number of images from multi-sensors simultaneously. The incorporated multi-sensors are frame, push broom, and whisky broom cameras. The limits and problems of push-broom or whisky broom sensor models can be compensated by combined triangulation with other sensors The reconstructed object space from multi-sensor triangulation is more accurate than that from a single model. Experiments conducted in this study show the more accurately reconstructed object space from multi-sensor triangulation.