TLC condition was developed for its simple separation and quantitative analysis of lactic acid. Rapid and clear separation of lactic acid by silica gel TLC plate was obtained by using nitromethane : 1-propanol : $H_2O$ (2 : 5 : 1.5, v/v/v) and a suitable dipping solution of 40 mg bromocresol purple in 100 mL 5% ethanol (pH 10.0). The lactic acid was shown as a bright yellow spot on a light cinnabar background. The quantitatively detectable concentration range of lactic acid was between 0.5 and 4% with 99.4%, confidence. Quantitative TLC analysis result was confirmed with HPLC and with enzymatic Quantitative analysis methods (by using lactate dehydrogenase).
Background and objective: This study investigated plant type and color preference and conducted a psychological assessment for gardening activities of firefighters in a high-risk occupational group. Methods: A survey was conducted on 117 firefighters aged 39.01±9.17 in Sunchang in March 2020 to examine their preferences in plant type and color and conduct a psychological assessment of firefighters on gardening activities. Results: For plant experience and awareness, plant-related event showed the highest score, followed by plant preference tendency and plant growing experience. The level of plant gardening activities was higher among older age and higher job position. As a result of examining the quality of life according to the demographic characteristic with WHOQOL-BREF (World Health Organization Quality of Life Assessment Instrument), it was found that the quality of life was higher among the 20s-30s than 50s, firefighter position than fire lieutenant or higher, those with higher average monthly income, and the singles group than married (p < .05). The administration group showed lower scores than the fire suppression group working in the field, showing statistical significance. Most firefighters showed high preference for herbal plants among the 12 types of flowering plants, in the order of Rosmarinus officinalis>Lavandula spp.>Mentha piperita, Calendula officinalis. Pelargonium spp. was preferred the least out of the flowering plants, Lycopersicon esculentum was preferred the most out of 12 types of vegetable plants, while Angelica acutiloba was preferred the least statistical significance. For the preference of plant colors, yellow was the most preferred color, followed by white, blue, orange, red, and green, showing statistical significance. For the psychological survey responses of emotion words toward 6 plants colors, it was found that 'bright' was the emotion felt most strongly by the white, yellow, and orange groups, 'calm' and 'comfortable' by the green and blue groups, and 'fancy' by the red group. Conclusion: The effect of agro-healing programs can be increased by using mostly yellow plants when creating indoor healing gardens at the workplaces of fire officials in a high-risk occupational group, and using herbal plants and Lycopersicon esculentum in the gardens for agro-healing activities.
This study examines the rotor characteristics of Guybang culture (women's living in Joseon) relics which the life images and mental world of women in Joseon if expressed to prepare rotor data to be utilized in modern design and information for color identity establishment of Korean women culture. The result of this study is as following: 1. From 176 pieces of relic photos, total of 1380 color chips were extracted. For color analysis, 1368 units (99.1%) of color data excluding achromatic 12 units (0.9%) among 1380 units of colors were used. With the standard of 10 color wheel of Munsell Color System, the result of studying distributions according to frequency, red was the highest by 25.9%, yellow was 21.4% in women's goods in Joseon period which the ratio of red and yellow was 473%. Other than that, GY 14.6%, YR 11.3% and RP 11.1% followed next which is studied that the warm colors that conveys enjoyable and glamorous feelings composed 84.3%; 2. As a result of tone analysis, high value low chroma ltg color showed highest distribution of 20% and d was 12.2%, b was 11.7% and sf was 8.8%. Generally, it showed high frequency in high value cocors such as ltg, sf and b of 40.5%t and in low chroma (ltg) and medium chroma (d, sf) of 41% and colors in bright and calm image was used the most. 3. As a result of analyzing relationship between hue & tone, the ltg tone of Y close to white in traditional color sensibility showed the highest frequency of 9.6% and ltg tone of GY(6.4%), b tone of RP(6.1%), and v(5.4%), b(5.4%), dp(5.3%) of R followed next. 4. As a result of analyzing the characteristics of coloring, it showed that in case of coloring contrasting hues, it is adjusted similar tones and in case of coloring less contrasting hue, the difference in tone is adjusted to be rather large to make harmonized coloring. Moreover, in case of coloring similar hues, the ratio of using same tone and similar tone was large which the coloring of calm image and dynamic image is all used.
Antifibrotic drugs could be delayed or inhibited massive collagen deposition in liver tissue or inhibited collagen synthesis. we investigated antifibrotic effects by several herbs(Schisnadra chinensis, Ganoderma japonicum, Sedum sarmentosum, Alisma canaliculatum, Plantago asiatica) extract with observations of collagen accumulation in liver tissue and collagen synthesis in sera. Rats were used for experimental animal that were devided 3 groups(sham control, BDL/S, BDL/S-ER). Rats were operated for inducing liver fibrosis(cirrhosis) by bile duct obstruction. Several herbs were prepared by water extraction and were applicated p. o. $3ml/day$ during 4 weeks. After observation period, rats were sacrificed and liver tissue and sera were collected. In result, the mortality of rats was 35% in BDL/S group and 20% in BDL/S-ER The color of bile juice in BDL/S-ER was bright yellow and murky yellow in BDL/S group. The significantly lower weight of liver($16.21g{\pm}5.3,\;20.58{\pm}2.4$) and spleen($1.96g{\pm}0.96,\;3.93{\pm}0.21$) were shown in BDL/S-ER than that of BDL/S(p<0.05) group. The value of collagen in liver tissue(25.7%) in BDL/S-ER was observed significantly lower than that of BDL/S group (Tab. 2). AST. ALT, ALP, t-bilirubin, BUN levels were low in BDL/S-ER as compared with those of BDL/S group, but the significance was not proven. The trichrome stained liver tissue in BDL/S-ER group was observed mild bile duct proliferation and fibrosis compared with BDL/S group. In conclusion, natural products inhibited new collagen synthesis and delayed massive collagen deposition in liver tissue, so that they have noticeable antifibrotic effects in experimental liver fibrosis(cirrhosis).
The gesneriaceous perennial plant Titanotrichum oldhamii has beautiful foliage and attractive bright yellow flowers. However, breeding of T. oldhamii by conventional sexual hybridization may be difficult because sexual reproduction of this species is very rare. In the present study, plant regeneration systems via both direct and indirect formation of adventitious shoots from leaf explants were established as the first step toward breeding T. oldhamii by using biotechnological techniques. Adventitious shoots were formed efficiently on medium containing $0.1mg\;l^{-1}$ benzyladenine. Histological observation showed that shoot formation on this medium occurred directly from leaf epidermal cells without callus formation. On the other hand, leaf explants formed calluses on medium containing $0.1mg\;l^{-1}$ 2,4-dichlorophenoxyacetic acid. The calluses could be maintained by monthly subculturing to fresh medium of the same composition. When the calluses were transferred to plant growth regulator-free medium, they formed adventitious shoots. Directly and indirectly formed shoots rooted well on medium containing $0.1mg\;l^{-1}$ indole-3-butyric acid. Plantlets thus obtained were successfully acclimatized and grew vigorously in the greenhouse. Flow cytometry analysis indicated that no variation in the ploidy level was observed in plants regenerated via direct shoot formation, whereas chromosome doubling occurred in several plants regenerated via indirect shoot formation. Regenerated plants with the same ploidy level as the mother plants showed almost the same phenotype as the mother plants, whereas chromosome-doubled plants showed apparent morphological alterations: they had small and crispate flowers, and round and deep green leaves.
Depending on the launch of the emotional generation, many researches developing the automobile preference color which reflects the preference color of the consumers are advanced. This study investigate preferred colors and emotional image of the car color reflecting the product design and consuming psychology. According to surveyed ages and gender, there are eight categories (women/20 's, 30 "s, 40 's, and 50 's and men/20 's, 30 "s, 40 's, and 50 's) based on the 30 persons. Preferred color is black, white, sky blue, purple, pink, and orange order. If black, white and gray are excluded, remained in the order of blue, yellow, and purple. There are big deferences on preference color when emotional preference color of cars is showed or not. Practically, the preference color is following in sensitivity. However the car image is recognized actually with conservativeness, they hesitates selection of the bright chromatic color car.
The egg capsule of Hongeo koreana is newly described based on two egg capsules extracted from the uterus of a female collected near the type locality, the southwestern coast of the Korean Peninsula. The egg capsule of H. koreana has the following characters: medium sized, measuring 124.5~133.7 mm in total length including horns, 102.8~109.4 mm in length excluding horns, and 41.0~42.2 mm in maximum width; asymmetrical, with the egg capsule more convex ventrally in lateral view; the surface relatively smooth, with longitudinal striations distinct, and covered with masses of sticky silky fibers; anterior and posterior aprons distinct; all four horns short, tube-like in shape, elliptical in cross section, anterior horns recurved inwardly; respiratory fissure present at tip of each of the four horns. The color of the fresh capsule is bright brown.
Journal of the Korea Fashion and Costume Design Association
/
v.18
no.2
/
pp.115-125
/
2016
This study analyzes the characteristics of the motifs and colors in flower and bird paintings which contain and present Korea's unique aesthetics based on its richest data among all other types of Korean folk paintings. With a theoretical exploration of folk paintings and an analysis of the motifs and colors, textile design with an aim to present highly valued korean modern fashion design was developed. Two hundred and seventy flower and bird paintings were selected from relevant materials, resulting in an extraction of 5,068 colors. A palette of representative colors was made based on densely distributed hues and tones. The research can be summarized as follows: Flower and bird paintings contain flowers, birds, trees and animals and the motifs in the paintings stand for spirituality, philosophies from different times and other symbols of our nation. Each of the motifs in these paintings is a prayer for good luck, especially conjugal harmony and fecundity to bring about happiness and richness. Colors in flower and bird paintings are characteristic of the order: YR(25.5%)>R(19.2%)>Y(10.6%)>PB(9.2%)>G(7.9%), with low-chromatic YR and highly bright R taking up a larger percent. The order of tones is: d(13.5%)>dk (10.5%)>s(10.4%)>dkg(10.0%)>sf(9.1%)and the tone is dark in general. YR, taking up the largest percent, was dull, light, and soft. As for the achromatic colors, the order is: W(5.2%)>Gy(1.9%)>Bk(0.7%). Based on the above analyzed color data, those colors which were high in their hue and tone were extracted to present representative colors. In this way, representative colors like yellow, yellowish red, red, green, and purplish blue were extracted. This was the basis to present motifs and colors originating from subjects of folk paintings in various patterns using Illustrator CS6, to create modern fashion design.
The Proceedings of the Korean Institute of Illuminating and Electrical Installation Engineers
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v.11
no.3
/
pp.63-70
/
1997
The objective of this study was to investigate the color preference and the recognition of color names among college students. The study revealed the following: 1) The most favorite colors included blue, purple, white and green in that order; and the least favorite colors included achromatic colors, ocher and red in that order. 2) Brown was the favorite color in furniture while red was the disliked color; In colthing, white was preferred while red was disliked; Red was appropriate for accent colors, and bright colors were for product packages. 3) In regards with seasonal image, yellow, blue, brown and white were associated with spring, summer, fall and winter, respectively; Blue represented self-image most. 4) Black, grey, red and green in that order showed the higest recognitions in color names while indigo, crimson, deep carmine and havy blue showed the lowesR ; Color name recognitions showed the order of achomatic, neutial, warm and cool colors.
Aflatoxins is a chemically diverse group of toxic secondary metabolites that are produced by fungi and often occur in agricultural commodities. Because of their wide range of toxic effects, Aflatoxins cause severe economic losses to farmers and livestock producers and pose a health to human consuming contaminated foods. Long term prospects for biotechnological control of Aflatoxins require elucidation of the specific steps and regulation of their biosynthetic pathways . Aflatoxin determinations can be approached many ways. It is essential to safely handle all experimental materials associated with aflatoxin analysis or aflatoxigenic fungi Visual screening of suspect samples, base on the presence of conidial head of the aspergillus flavus group, and screening samples for the presence of bright greenish yellow flourescence are not chemical tests and such screening techniques may allow aflactoxin contaminated lots into commerce. Microcolumn screening procedures should always be used in conjunction with a quantitative method. Several thin layer chromatography(TLC) and high performance liquid chromatography(HPLC) methods are suitable for quantitation and are in general use. Immunochemical Methods such as the ELISA or affinity column chromatography methods are being rapidly developed. The chemical and immunochemical methods can be reliable if care is taken, using suitable controls and personnel that are well trained . All analytical laboratories should stress safety and include suitable analytical validation procedure. Especially a worldwide enquiry was undertaken in recent to obtain up-to-date information about aflatoxin legislation in as many countries of the world as possible. The information concerns aflatoxin in foodstuffs. aflatoxin MI in dairy products, aflatoxins in animal feedstuffs. Limits and regulations for aflatoxin have been expended in recent with more countries having legislation on subject, more products, and more aflatoxins covered by this legislation.
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