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Simple and Quantitative Analysis Method for Lactic Acid by TLC  

최미화 (전남대학교 물질·생물화학공학과)
조갑수 (전남대학교 분자생물공학과)
강희경 (공업기술연구소)
윤종선 (전남대학교 응용화학공학부)
서은성 (전남대학교 물질·생물화학공학과)
류화원 (전남대학교 응용화학공학부)
장세효 ((주)진성 SMR)
윤승헌 (미국 아이오와주립대학교 생화학과)
김도만 (전남대학교 응용화학공학부)
Publication Information
KSBB Journal / v.18, no.1, 2003 , pp. 70-73 More about this Journal
Abstract
TLC condition was developed for its simple separation and quantitative analysis of lactic acid. Rapid and clear separation of lactic acid by silica gel TLC plate was obtained by using nitromethane : 1-propanol : $H_2O$ (2 : 5 : 1.5, v/v/v) and a suitable dipping solution of 40 mg bromocresol purple in 100 mL 5% ethanol (pH 10.0). The lactic acid was shown as a bright yellow spot on a light cinnabar background. The quantitatively detectable concentration range of lactic acid was between 0.5 and 4% with 99.4%, confidence. Quantitative TLC analysis result was confirmed with HPLC and with enzymatic Quantitative analysis methods (by using lactate dehydrogenase).
Keywords
TLC (Thin layer chromatography); lactic acid; bromocresol purple; Tuantitative analysis method; separation;
Citations & Related Records
Times Cited By KSCI : 4  (Citation Analysis)
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[ Choi, H. J.;C. I. Cheigh;S. B. Kim;J. C. Lee;D. W. Lee;S. W. Choi;J. M. Park;Y. R. Pyun ] / Int. J. Syst. Evol. Microbiol.
11 /
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12 Isolation and characterization of lactic acid bacteria from feces of newborn baby and from dongchimi /
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[ Park, C. I.;C. H. Lee ] / The theory and practice of HPLC
15 Lactic acid production and carbon catabolite repression from single and mixed sugars using Enterococcus faecalis RKY1 /
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