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Clinical Results of Distal Femoral Osteotomy for Treatment of Grade 4 Medial Patella Luxation with Concurrent Distal Femoral Varus in Small Breeds Dogs: 13 Cases

  • Roh, Yoon-Ho;Jung, Jin-Ho;Lee, Je-Hun;Jeong, Jae-Min;Jeong, Seong Mok;Lee, HaeBeom
    • Journal of Veterinary Clinics
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    • v.37 no.3
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    • pp.135-140
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    • 2020
  • The purpose of this study was to determine the outcome of distal femoral osteotomy for distal femoral varus and medial patellar luxation (MPL) grade 4 in small-breed dogs. Radiographs and medical records were reviewed to collect data and plan the surgery in small-breed dogs with MPL grade 4. Computed tomography (CT) imaging was also performed in cases of severe bone deformities. Signalment, weight, medial patellar luxation and lameness grade, radiographic bone union, complications, pre- and postoperative femoral varus angle, passive range of motion, static weight bearing distribution and visual analogue scale scores were recorded. Thirteen corrective distal femoral osteotomies were performed with ancillary and additional procedures in 9 dogs; 4 dogs had staged bilateral procedures; and four stifles were suspected to have partial or complete rupture of the cranial cruciate ligament. One stifle underwent patellar groove replacement. The mean ± SD pre- and postoperative femoral varus angles were 109.15°± 3.71° and 96.30°± 2.97°, respectively. Significant improvements in passive range of motion, thigh circumference and visual analogue scale (VAS) scores were observed. There was no reluxation of the patella. This study suggests that distal femoral osteotomy with traditional and additional procedures provided satisfactory outcomes in patient healing and functional recovery in small-breed dogs with excessive femoral varus angles.

Comparison of growth performance of Berkshire purebreds and crossbreds sired by Hereford and Tamworth breeds raised in alternative production system

  • Park, Hyeon-Suk;Spann, Kristal;Whitley, Niki;Oh, Sang-Hyon
    • Asian-Australasian Journal of Animal Sciences
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    • v.30 no.9
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    • pp.1358-1362
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    • 2017
  • Objective: The objective of the present study was to compare growth performance of Berkshire purebred pigs (BB), Hereford (HB) and/or Tamworth (TB) sired Berkshire crossbred pigs reared in a hoop structure in two experiments. Methods: In the first experiment, BB was compared to TB while HB and TB were compared in the second. Body weights (BW) were recorded at 3 days of age and every 28 days from birth until 140 days of age. There was no significant difference between the BW of BB and TB, but HB was heavier than TB by 84 days of age. Least square means of average daily gain (ADG) were evaluated using one-way analysis of variance. Results: The mean parity (${\pm}$standard deviation) of the sows was $3.42{\pm}2.14$ and a total of 45 farrowing occurred from year 2012 to 2014. The mean number of total born, number born alive, number of mummies, and number weaned were $9.23{\pm}2.52$, $7.87{\pm}2.53$, $0.04{\pm}0.21$, and $5.94{\pm}2.74$, respectively. Parity did not have a significant effect on the growth performance of the pigs. For BB and TB, there was only one time frame in which there was a significant difference in the ADG: between 28 and 56 days of age. For HB and TB, the overall ADG of HB was significantly greater than the total ADG of TB. Conclusion: The breed of the sire did not affect the growth performance of the progeny between Berkshire purebreds and Tamworth${\times}$Berkshire crossbreds. The breed of the sire did have an effect between Hereford and Tamworth sired Berkshire crossbreds (p<0.05). The Hereford sired pigs were found to have increased growth performance compared to Tamworth sired.

Grazing Behaviour of Saanen and Toggenburg Goats in Sub-Humid Tropical Conditions of Kenya

  • Njoka-Njiru, E.N.;Ojango, J.M.;Ambula, M.K.;Ndirangu, C.M.
    • Asian-Australasian Journal of Animal Sciences
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    • v.14 no.7
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    • pp.951-955
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    • 2001
  • The behaviour of 6 Toggenburg and 6 Saanen female goats, grazing on natural vegatation in a modified sub-humid tropical environment of Kenya, was studied during the dry (January and February) and wet (April and May) seasons in 1997. The two exotic breeds grazed for 12 h daily. Observation on feeding, standing, lying and ruminating was done chronometrically at five minute intervals between 0600 and 1800 h. These grazing times were based on the current practice of small holder farmers. During the dry period, the average feeding, standing, lying and ruminating time per 12 h period for Saanens was 5.63, 4.57, 1.80 and 1.50 h respectively while that for Toggenburgs was 7.26, 3.21, 1.53 and 1.96 h, respectively. In the wet(Green) season, the mean feeding, standing, lying and ruminating time for Saanens was found to be 5.08, 4.63, 2.29 and 0.72, respectively.Toggenburgs spent more time feeding (p<0.05) than Saanens in both seasons. It was also observed that standing occured more often in Saanens than the Toggenburgs in the dry season. During the wet period, Saanens ruminated significantly longer than the Toggenburgs. The health status of the animals was determined by analyzing the respiratory frequency, heart rate, rectal temperature, hemoglobin, erythrocytes and leucocytes of the experimental animals. All the clinical and physiological parameters were within the normal physiological range of healthy goats. It was concluded that differences in the grazing behaviour of Saanens and Toggenburgs in the modified tropical environment of Kenya, do exist.

Calf Rearing Systems in Smallholder Dairy Farming Areas of Zimbabwe : A Diadnostic Study of the Nharira-Lancashire Area

  • Mandibaya, W.;Mutisi, C.;Hamudikuwanda, H.
    • Asian-Australasian Journal of Animal Sciences
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    • v.12 no.1
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    • pp.68-76
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    • 1999
  • A formal survey was carried out in Nharira-Lancashire areas located in Chivhu to assess the calf rearing systems practiced in smallholder dairy farming areas of Zimbabwe. A total of 47 farmers, collectively owning 305 cows and 194 calves of various breeds, participated in the survey. All the farmers allowed their calves to suckle their dams all day to obtain colostrum. The colostrums intake period was significantly (p < 0.05) shorter (5.2 vs 4.1 days) in the small scale commercial area (SSCA) compared to communal area (CA). Milk was first sold to the Nharira-Lancashire Milk Centre a day after the colostrum intake period ended. Most of the CA (91.3%) and SSCA (77.8%) farmers penned their cows and calves together at night during the colostrum intake period. Thereafter the calves were penned separate from their dams. After colostrum intake, two types of calf suckling systems were practised; twice a day suckling and twice a day then changed to once a day suckling. In both systems, suckling was allowed for 30 minutes after the cows had been hand milked. There was no significant (p < 0.05) difference in the mean weaning age of calves between the CA and SSCA (5.8 vs 5.4 months). The most common weaning method was through separation of the calves from the dams. The limitaitions to calf production in Chivhu were the prohibitively high costs of calf meals, poor feed resources during the dry season, a general lack of knowledge on calf rearing diseases and inappropriate calf housing.

Identification of a Novel Single Nucleotide Polymorphism in Porcine Beta-Defensin-1 Gene

  • Pruthviraj, D.R.;Usha, A.P.;Venkatachalapathy, R.T.
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.3
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    • pp.315-320
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    • 2016
  • Porcine beta-defensin-1 (PBD-1) gene plays an important role in the innate immunity of pigs. The peptide encoded by this gene is an antimicrobial peptide that has direct activity against a wide range of microbes. This peptide is involved in the co-creation of an antimicrobial barrier in the oral cavity of pigs. The objective of the present study was to detect polymorphisms, if any, in exon-1 and exon-2 regions of PBD-1 gene in Large White Yorkshire (LWY) and native Ankamali pigs of Kerala, India. Blood samples were collected from 100 pigs and genomic DNA was isolated using phenol chloroform method. The quantity of DNA was assessed in a spectrophotometer and quality by gel electrophoresis. Exon-1 and exon-2 regions of PBD-1 gene were amplified by polymerase chain reaction (PCR) and the products were subjected to single strand conformation polymorphism (SSCP) analysis. Subsequent silver staining of the polyacrylamide gels revealed three unique SSCP banding patterns in each of the two exons. The presence of single nucleotide polymorphisms (SNPs) was confirmed by nucleotide sequencing of the PCR products. A novel SNP was found in the 5'-UTR region of exon-1 and a SNP was detected in the mature peptide coding region of exon-2. In exon-1, the pooled population frequencies of GG, GT, and TT genotypes were 0.67, 0.30, and 0.03, respectively. GG genotype was predominant in both the breeds whereas TT genotype was not detected in LWY breed. Similarly, in exon-2, the pooled population frequencies of AA, AG, and GG genotypes were 0.50, 0.27, and 0.23, respectively. AA genotype was predominant in LWY pigs whereas GG genotype was predominant in native pigs. These results suggest that there exists a considerable genetic variation at PBD-1 locus and further association studies may help in development of a PCR based genotyping test to select pigs with better immunity.

Line${\times}$Tester Analysis of Certain Quantitative Traits in Silkworm Bombyx mori L. under Optimum and Stress Rearing Conditions

  • Rao, P.Sudhakara;Datta, R.K.;Kumari, K.M.Vijaya;Palit, A.K.;Bhat, S.A.
    • International Journal of Industrial Entomology and Biomaterials
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    • v.8 no.1
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    • pp.107-112
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    • 2004
  • The combining abilities in the 5 newly evolved thermo tolerant breeds viz., SR6, SR7, SR8 SR9 and SR10 of silkworm Bombyx mori L. and their 15 hybrids were made in a line${\times}$tester crossing programme. Data were analysed for seven quantitative traits i.e., pupation rate, cocoon yield, cocoon weight, cocoon shell weight, cocoon shell ratio, filament length and raw silk percentage under optimum room temperature $(25{\pm}{1^{\circ}C})$ conditions (In case of high temperature $(36{\pm}{1^{\circ}C})$ stress conditions five economic traits except filament length and raw silk percentage) with 3 widely adapted testers i.e., KA, CSR2 and CC1 as lines (females) and testers (males) respectively. The performance at high temperature and low humidity conditions aye only taken into consideration for selecting the best lines/hybrids. Among the lines SR6 exhibited positive General combining ability (GCA) effects for pupation rate, cocoon yield, cocoon weight and cocoon shell ratio traits, followed by SR7 for pupation rate, cocoon yield and cocoon shell weight and cocoon shell ratio. Among testers, KA exhibited positive GCA effects for two quantitative traits cocoon yield, cocoon weight and CSR2 for cocoon shell weight and cocoon shell ratio under adverse temperature conditions. The hybrid SR6${\times}$CC1 and SR7${\times}$CSR2 exhibited significant positive Specific combining ability (SCA) effects for majority of the traits in high temperature stress conditions of rearing. The better parent value of heterosis(Heterobeltiosis) was exhibited by the hybrid SR6${\times}$CC1 for pupation rate, cocoon yield, cocoon weight and cocoon shell weight and SR7${\times}$CSR2 for all the trails evaluated under high temperature conditions. Based on the results, the lines SR6 and SR7 was judged as best combiners and the hybrids SR6${\times}$CC1 and SR7${\times}$CSR2 can be selected for commercial exploitation in tropical climate.

Single Nucleotide Polymorphism Analysis of the COI Gene in Korean Native Chicken (한국재래닭 COI 유전자의 단일염기다형 분석)

  • Jin, S.D.;Seo, D.W.;Sim, J.M.;Baek, W.K.;Jung, K.C.;Jang, B.K.;Choi, K.D.;Lee, J.H.
    • Korean Journal of Poultry Science
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    • v.36 no.1
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    • pp.85-88
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    • 2009
  • One of the mitochondrial genes, called cytochrome c oxidase I (COI), has been widely used for the species identification (called bio-barcode) in birds. In this study, the bio-barcode has been applied to chicken breeds in Korea whether it also can be used as a molecular marker for breed identification. Data indicated that Korean native chicken has the mixed SNP (single nucleotide polymorphism) patterns between White Leghorn (Layer) and Cornish (Broiler) and ultimately, it can not be used as the marker for breed identification. However, this result indicates the mixed use of the Korean native chicken, since it has been used for dual purpose for producing meat and egg for a long time. In order to use as a marker for species identification, more reliable mitochondrial and/or nuclear DNA markers need to be developed.

Corporate Vertical Coordination, and Farmer's Land Use in Contract Farming Relations between Discount Store and Contract Farmers: A Case Study of the Watermelon Farming District in Buyeo-gun, Chungcheongnam-do (대형마트와 농가 간 계약생산에서 계약기업의 수직적 조정과 계약농가의 토지이용: 충청남도 부여군 일대 수박 주산지를 사례로)

  • Jang, YoungJin
    • Journal of the Economic Geographical Society of Korea
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    • v.19 no.4
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    • pp.712-728
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    • 2016
  • This study examines the foundations of contract farming, the extent of corporate vertical coordination, and farmer's land use in regards to the contract farming between discount store and watermelon farmers in Buyeo-gun, Chungcheongnam-do. In the above case, contract farming is led by the group of producers. As the corporate's role is limited due to the specification of watermelon breed, contract farming takes the form of procurement contract with restrained levels of corporate vertical coordination. Thus, it has become common for contract farmers to freely enter and leave contracts and sign contracts with multiple clients. Furthermore, farmers participate in contract farming by using contract breeds in existing crop rotation systems. Through this process, watermelon farming is undergoing a process of diversification via the spread of contract varieties in this region.

Carcass Traits and Meat Quality of Prestice Black-Pied Pig Breed

  • Matousek, Vaclav;Kernerova, Nadezda;Hysplerova, Klara;Jirotkova, Dana;Brzakova, Michaela
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.8
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    • pp.1181-1187
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    • 2016
  • The objective of the study was to evaluate fattening performance, carcass value and meat quality in pigs of Prestice Black-Pied breed in relation to slaughter weight (SW) and gender (barrows and gilts, resp.). Pigs were divided into weight categories: SW1 (75 to 99.9 kg), SW2 (100 to 109.9 kg) and SW3 (110 to 130 kg) and all individual traits were analyzed by the general linear model procedure (SAS 9.3). Average SW of each weight group was as follows: SW1 94.2 kg, SW2 105.8 kg, and SW3 115.2 kg. Differences among average backfat thickness of 36.07 mm in SW1, 40.16 mm in SW2, and 43.21 mm in SW3 were significant (p<0.01). Lean meat content was 48.94% (SW1), 48.78% (SW2), and 48.76% (SW3). Pigs were slaughtered at average weight of 105.7 kg for barrows and 104.4 kg for gilts. Average backfat thickness for barrows was 40.90 mm and 38.72 mm for gilts (significant difference p<0.05). Lean meat content was 48.75% in barrows and 48.91% in gilts. The values of pH45, characterizing the meat of very good quality. The loin in SW3 was darker than the muscles of SW1 and SW2. Drip loss was the lowest in SW1 (1.96%), compared to the highest drip loss in SW3 (2.59%). Content of intramuscular fat was 2.68% in SW3, 2.79% in SW2, and SW1 had the lowest content 2.47%. The values of pH45, colour lightness and drip loss were similar in both genders. However barrows had higher intramuscular fat content by 0.31% than gilts (p<0.05).

Association of Chicken Growth Hormones and Insulin-like Growth Factor Gene Polymorphisms with Growth Performance and Carcass Traits in Thai Broilers

  • Nguyen, Thi Lan Anh;Kunhareang, Sajee;Duangjinda, Monchai
    • Asian-Australasian Journal of Animal Sciences
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    • v.28 no.12
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    • pp.1686-1695
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    • 2015
  • Molecular marker selection has been an acceptable tool in the acceleration of the genetic response of desired traits to improve production performance in chickens. The crossbreds from commercial parent stock (PS) broilers with four Thai synthetic breeds; Kaen Thong (KT), Khai Mook Esarn (KM), Soi Nin (SN), and Soi Pet (SP) were used to study the association among chicken growth hormones (cGH) and the insulin-like growth factor (IGF-I) genes for growth and carcass traits; for the purpose of developing a suitable terminal breeding program for Thai broilers. A total of 408 chickens of four Thai broiler lines were genotyped, using polymerase chain reaction-restriction fragment length polymorphism methods. The cGH gene was significantly associated with body weight at hatching; at 4, 6, 8, 10 weeks of age and with average daily gain (ADG); during 2 to 4, 4 to 6, 0 to 6, 0 to 8, and 0 to 10 weeks of age in $PS{\times}KM$ chickens. For $PS{\times}KT$ populations, cGH gene showed significant association with body weight at hatching, and ADG; during 8 to 10 weeks of age. The single nucleotide polymorphism variant confirmed that allele G has positive effects for body weight and ADG. Within carcass traits, cGH revealed a tentative association within the dressing percentage. For the IGF-I gene polymorphism, there were significant associations with body weight at hatching; at 2, 4, and 6 weeks of age and ADG; during 0 to 2, 4 to 6, and 0 to 6 weeks of age; in all of four Thai broiler populations. There were tentative associations of the IGF-I gene within the percentages of breast muscles and wings. Thus, cGH gene may be used as a candidate gene, to improve growth traits of Thai broilers.