• Korean Journal of Environment and Ecology
• /
• v.29 no.2
• /
• pp.162-173
• /
• 2015

The current study analyzed the home range size of the Common Kestrel (Falco tinnunculus) by observation using the natural markings in the suburban area of Busan from December 2009 to May 2010. In all, 26 individual kestrels were observed in the study area, of which 14 individual kestrels were frequently observed (more than five times) and 12 were observed less frequently (less than five times). Mean home range sizes of the 14 frequently observed kestrels were estimated using two different methods (minimum convex polygon (MCP) method and fixed kernel (FK) method): 34.49 ha(2.74-163.96 ha) by 100% MCP, 41.35 ha(3.54-99.02 ha) by the 95% FK, 14.33 ha(0.20-31.57 ha) by the 75% FK and 5.75 ha(0.52-14.74 ha) by the 50% FK. The mean home range size was larger for males than for females estimated at 51.50 ha(15.04-163.96 ha) and 11.80ha(2.74-24.48 ha), respectively, by the 100 % MCP method and 8.02 ha(2.38-14.74 ha) and 2.72 ha (0.52-4.28 ha), respectively, by the 50 % Fixed Kernel method. The home range sizes by sexes in breeding pairs differed during the wintering and breeding seasons; the sizes decreased among females but increased among males during the breeding season. Such differences of home range sizes between sexes could be attributed to parental role division during the breeding season. Females stay in the nest or next to it during laying, incubating, and feeding activities, and males go far away from their nest sites to forage areas for their fledglings for most of the breeding period.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.4
• /
• pp.577-581
• /
• 2007

The effects of intramuscular fat on the sensory characteristics of M. longissimus dorsi in Japanese Black steers were investigated by a trained analytical panel (average 13.4 panelists). Five sets (10 head) of artificial identical twins were divided into 2 groups, high level of intramuscular fat group (HG) which utilized the fattening method of increasing intramuscular fat and low level of intramuscular fat group (LG) which did not use the above method. Regarding M. longissmus dorsi which was produced for use in the sensory evaluation, crude fat contents of HG and LG was 25.8% and 23.2% respectively (p<0.05). Warner-Bratzler shear force, water holding capacity, cooking loss and fatty acid composition of HG and LG were similar between the two groups. M. Longissimus dorsi taken from HG and LG were tested for their sensory characteristics by a trained panel. HG was given higher points for juiciness than LG (p<0.05). There were no significant differences for tenderness and flavor between the two groups. Overall acceptability which synthesized each of the sensory characteristics of HG and LG were 5.04 and 4.69 points respectively (p = 0.05). These results suggested that juiciness increased with the increase of intramuscular fat, and this raised the overall acceptability of M. longissimus dorsi.

• Asian-Australasian Journal of Animal Sciences
• /
• v.20 no.8
• /
• pp.1169-1173
• /
• 2007

The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) ($A{\rightarrow}G$) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 $F_2$ reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.

• Asian-Australasian Journal of Animal Sciences
• /
• v.30 no.2
• /
• pp.149-153
• /
• 2017

Objective: This study was to determine the relationship between estimated breeding value and phenotype information after farrowing when juvenile selection was made in candidate pigs without phenotype information. Methods: After collecting phenotypic and genomic information for the total number of piglets born by Landrace pigs, selection accuracy between genomic breeding value estimates using genomic information and breeding value estimates of best linear unbiased prediction (BLUP) using conventional pedigree information were compared. Results: Genetic standard deviation (${\sigma}_a$) for the total number of piglets born was 0.91. Since the total number of piglets born for candidate pigs was unknown, the accuracy of the breeding value estimated from pedigree information was 0.080. When genomic information was used, the accuracy of the breeding value was 0.216. Assuming that the replacement rate of sows per year is 100% and generation interval is 1 year, genetic gain per year is 0.346 head when genomic information is used. It is 0.128 when BLUP is used. Conclusion: Genetic gain estimated from single step best linear unbiased prediction (ssBLUP) method is by 2.7 times higher than that the one estimated from BLUP method, i.e., 270% more improvement in efficiency.

• Journal of Microbiology and Biotechnology
• /
• v.24 no.9
• /
• pp.1162-1169
• /
• 2014

Glyphosate is the active component of the top-selling herbicide, the phytotoxicity of which is due to its inhibition of the shikimic acid pathway. 5-Enolpyruvylshikimate-3-phosphate synthase (EPSPS) is a key enzyme in the shikimic acid pathway. Glyphosate tolerance in plants can be achieved by the expression of a glyphosate-insensitive aroA gene (EPSPS). In this study, we used a PCR-based two-step DNA synthesis method to synthesize a new aroA gene ($aroA_{R.\;leguminosarum}$) from Rhizobium leguminosarum. In vitro glyphosate sensitivity assays showed that $aroA_{R.\;leguminosarum}$ is glyphosate tolerant. The new gene was then expressed in E. coli and key kinetic values of the purified enzyme were determined. Furthermore, we transformed the aroA gene into Arabidopsis thaliana by the floral dip method. Transgenic Arabidopsis with the $aroA_{R.\;leguminosarum}$ gene was obtained to prove its potential use in developing glyphosate-resistant crops.

• Proceedings of the Korean Society of Crop Science Conference
• /
• 2017.06a
• /
• pp.67-67
• /
• 2017

We developed the breeding materials with diverse grain size and shape in japonica rice. Grain size and shape are important factors affecting consumer preference and choice. However, most of Korean japonica rice cultivars have small, short, and round grain. To diversify the grain size and shape of japonica rice, we conducted the breeding program using donor parents, Jizi1560 and Jizi1581. Jizi1560 and Jizi1581 are japonica germplasm with extremely large grain. Four crosses between the each donor parents and high yielding japonica rice cultivars, Deuraechan and Boramchan, were constructed and then anther culture method was applied. We obtained 290 doubled-haploid (DH) lines with appropriated morphological traits and selected 91 DH lines with diverse grain size and shape. The grain related-traits of the selected DH lines showed a higher diversity when compared with 319 cultivars developed by NICS (264 japonica, 13 black, and 32 Tongil type cultivars). We designated the selected DH lines, four parents, and Daeripbyeo 1, large grain japonica cultivar, as the breeding materials for further analysis. The breeding materials were classified into five groups, A to E, based on the grain-related traits. Group A (including Jizi1581) and Group B (including Daeripbyeo 1) showed similar grain width, whereas Group A exhibited longer grain length and heavier grain weight. Group C (including Deuraechan and Boramchan) showed shorter and rounder grain shape and smaller grain size than any other groups. Group D including solely Jizi1560 had extremely large grain, such as the longest grain length, width, and thickness and heaviest grain weight. Group E including only two DH lines had long and slender grain shape, so that showed the highest ratio of length to width. The grain size and shape of the breeding materials exhibited beyond the characteristics of previously developed Korean japonica cultivars. The breeding materials will be applied in the breeding programs to diversify the grain size and shape of japonica rice.

• Asian-Australasian Journal of Animal Sciences
• /
• v.33 no.9
• /
• pp.1507-1519
• /
• 2020

Objective: The current research was aimed to profile the transcriptomic picture of the peripheral blood lymphocytes (PBLs) associated with immunity in Chinese Holsteins supplemented orally with coated folic acid during the periparturient period. Methods: The total of 123 perinatal cows were selected for this study and divided into three groups; group A (n = 41, 240 mg/500 kg cow/d), group B (n = 40, 120 mg/500 kg cow/d) and group C (n = 42, 0 mg/cow/d) based on the quantity of folic acid fed. Three samples of PBLs were selected from each folic acid treated group (high, low, and control) and RNA sequencing method was carried out for transcriptomic analysis. Results: The analysis revealed that a higher number of genes and pathways were regulated in response to high and low folic acid supplementation compared to the controls. We reported the novel pathways tumor necrosis factor (TNF) signaling, antigen processing and presentation, Staphylococcus aureus infection and nuclear factor (NF)-kappa B signaling pathways) and the key genes (e.g. C-X-C motif chemokine ligand 10, TNF receptor superfamily member 1A, cluster difference 4, major histocompatibility complex, class II, DQ beta, NF-kappa-B inhibitor alpha, and TNF superfamily 13) having great importance in immunity and anti-inflammation in the periparturient cows in response to coated folic acid treatment. Conclusion: Collectively, our study profiled first-time transcriptomic analysis of bovine lymphocytes and compared the involved cytokines, genes, and pathways between high vs control and low vs control. Our data suggest that the low folic acid supplementation (120 mg/500 kg) could be a good choice to boost appropriate immunity and anti-inflammation as well as might being applied to the health improvement of perinatal dairy cows.

• Korean Journal of Fisheries and Aquatic Sciences
• /
• v.55 no.5
• /
• pp.549-556
• /
• 2022

The aim of this study was to evaluate the genetic parameters of growth traits for improvement breeding in olive flounder Paralichthys olivaceus at the 8^th generation of selective breeding in April 2021. Growth traits such as total length, body weight and condition factor at 11 months of age were measured for 7,508 individuals with confirmed paternity. Data were analyzed using the restricted maximum likelihood method applied to a multiple traits animal model. The effects of sex and family were significantly different across traits (P<0.05). The heritability values of total length, body weight and condition factor were estimated to be high as 0.479, 0.457, and 0.466, respectively. Correlation analysis between phenotypic and breeding values, indicated that the selection accuracy was 75.9-85.2% for all traits. To increase the selection accuracy for parent fish selection, the sex and pedigree characteristics that affect each trait should be considered. Moreover, further improvement of multiple traits can be achieved if the correlation between each trait is appropriately considered.

• Proceedings of the KSAR Conference
• /
• 2002.06a
• /
• pp.8-8
• /
• 2002

Goats have long history in Korea. But because of small holding, is there no systematic development for breeding and reproduction. The most of goats in Korea have some typical problems, which gradually appeared through inbreeding. The purpose of this study was to evaluate effects of hormonal treatment on estrus synchronisation in non-breeding seasons and to estimate economical aspects of caprine embryo transfer. Otherwise we would like to develop a suitable practical method of field embryo transfer, in order to obtain the more basical breeding materials for high-performance goat. (omitted)

• Journal of Ginseng Research
• /
• v.14 no.1
• /
• pp.50-56
• /
• 1990

Root-rot of ginseng caused by Fusarium solani is one of the most obstacles to ginseng cultivation. We evaluated some inoculating techniques of ginseng with Fusarium solani, for selection of disease resistant breeding lines. The most effective inoculating techniques evaluated were inserting toothpicks colonized by F. solani into the seedling roots in laboratory test and dusting seedlings with vermiculite after dipping in conidial sllspension and then replanting method in field test. The resistance to diseased by F. solani was lines of 82022 and 82066 in laboratory test. 82920-1 and 78093 in field test.